LOCUS       sequence-443582-        8477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid encodes luciferase which has siRNA binding sites in 
            3'UTR for detecting the siRNA silencing activity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8477)
  AUTHORS   Song J, Kobayashi Y, Asano Y, Sato A, Taniguchi H, Ui-Tei K
  TITLE     Knockdown of 15-bp Deletion-Type v-raf Murine Sarcoma Viral Oncogene
            Homolog B1 mRNA in Pancreatic Ductal Adenocarcinoma Cells Repressed 
            Cell Growth In Vitro and Tumor Volume In Vivo.
  JOURNAL   Cancers (Basel). 2022 Jun 28;14(13):3162. doi: 
            10.3390/cancers14133162.
  PUBMED    35804932
REFERENCE   2  (bases 1 to 8477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2022-06-28"; volume: "14(13):3162. doi"; pages: " 
            10.3390/cancers14133162"
COMMENT     Sequence Label: psiCHECK-1-BRAF_Mut1-SM
FEATURES             Location/Qualifiers
     source          1..8477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        188..545
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        188..379
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(211..231)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      396..531
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          615..747
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     730..749
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        792..810
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      814..823
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             820..1755
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    1878..1926
                     /note="synthetic polyadenylation signal"
     promoter        1959..2063
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2064..2132,2133..2924)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2282..2301)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3095..3683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3584..3603
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3837..3854
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3971..3992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4007..4012,4013..4030,4031..4037)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4045..4061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4050..4072
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4069..4085
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4069..4085
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    4134..4255
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4145..4164)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4199..4218
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4385..5407)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYSISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNSVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(5415..5462)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     5594..5613
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(5897..6148)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(7295..7360)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(7373..7450)
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MASVLTPLLLRGLTGSARRLPVPRAK"
     promoter        complement(7473..7491)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(7536..7739)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7565..7585)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7740..8119
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8291..8310
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
       61 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgg
      121 ctcgacagat ctgcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag
      181 gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
      241 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
      301 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
      361 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
      421 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
      481 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
      541 gcaaaaagct tgattcttct gacacaacag tctcgaactt aagctgcaga agttggtcgt
      601 gaggcactgg gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac
      661 tgggcttgtc gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg
      721 acatccactt tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag
      781 gctagagtac ttaatacgac tcactatagg ctagccacca tggcttccaa ggtgtacgac
      841 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
      901 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
      961 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
     1021 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
     1081 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
     1141 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
     1201 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
     1261 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
     1321 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
     1381 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
     1441 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
     1501 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
     1561 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
     1621 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
     1681 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
     1741 aagaacgagc agtaattcta ggcgatcgct cgagtccctt ttacaacaat gctcagctcc
     1801 cttttacaac aatgctcagc tcccttttac aacaatgctc agcgaattcg tttaaaccta
     1861 gagcggccgc tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt
     1921 tgtgtgagga tccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1981 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2041 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     2101 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     2161 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     2221 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     2281 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     2341 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2401 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2461 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     2521 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2581 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     2641 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2701 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     2761 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     2821 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     2881 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     2941 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     3001 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3061 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3121 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3181 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3241 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3301 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3361 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3421 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3481 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3541 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3601 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3661 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3721 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3781 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3841 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     3901 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     3961 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     4021 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     4081 atgaccatga ttacgccaag ctctagctag aggtcgacgg tatacagaca tgataagata
     4141 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     4201 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttggggt
     4261 gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga
     4321 aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtagta
     4381 cgtgctattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
     4441 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
     4501 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
     4561 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
     4621 cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
     4681 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
     4741 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
     4801 cactgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc
     4861 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
     4921 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
     4981 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
     5041 cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag
     5101 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gctataggtc aggctctcgc
     5161 tgaattcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat
     5221 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac
     5281 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt
     5341 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag
     5401 gctttttggc caaggaagtt cctatacttt ctagagaata ggaacttcgg aataggaact
     5461 tctaggtacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     5521 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     5581 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     5641 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     5701 ggggataccc cctagagccc cagctggttc tttccgcctc agaagttctg aggggtgtac
     5761 attttattgg aaaccttaaa tactgttcag aaagaatata tcttcaatca aggctcttgt
     5821 gcagcctaca cagaaaaatg aagctttttg ggttaggggc aaggagagag acagtacaga
     5881 ggacaaagac cccttatcac agcatcttga tcttattctc gccgttagaa tcctggatga
     5941 ccagggccca aggcttatac tcgggggcgt cagaggtcag cagcatcaca ttctcatctg
     6001 tggactcgtc gtaggcggtg tgcaccagga tgtcagactc tggcttgttg ccgatcactt
     6061 cctccacctc ctcaggcagc atcaggatgg actcctgaat gaccagctgt ttcccagtct
     6121 ccttctcaat gatgtcgctc agattagttg agccgccaga accttccggc ggcaccacgg
     6181 tcattttcgc gttttccggc agcagggtct cggtcatgtt cacgcaaaaa ccgcaggtgc
     6241 cttccgggtt gttgtggaac accagaccct cgctgatgct gttatcacgc atgaacaggg
     6301 cgctctgacc ctccacatga ccggcattgg catagtttgg gtacggggtt ggaccgccgc
     6361 tgctaaacac cttgctctcc agaccgcccg cgtcgttcac atagtagaag gtacccacgg
     6421 tctgaccatt gtaagctggc agctgcgggg cgctaatctg atacggaccc agggcgtagc
     6481 tgccggatcc acccaggcct ttcttcaccg catccagggc cggacgaccg cccaggcagg
     6541 ccagggccac caggtgatcg ttggtcaacg cggccaacgc cggatcaggc cggctcagct
     6601 gggccacaat gctttcgagc gcttgcttgc cgccatcgtg gctggcgata gccaccactt
     6661 ggtccggggt caggccatgg tcctggcaca gcaccggcaa cacccgctgc accgtttcga
     6721 gcgcttgctt gccgccatcg tggctggcga tagccaccac ttggtccggg gtcaggccat
     6781 ggtcctggca cagcaccggc aacagccgct gcaccgtttc gagcgcttgc ttgccgccat
     6841 cgtggctggc gatagccacc acttggtccg gggtcaggtt caggggggca ccggtcagag
     6901 cattacgcca ggcatgcaca gcttccacag cggtcacacc gccacgtttc gcgattttca
     6961 gcagctggcc ggtatccagc tgcagcggcg ggccacgcag ttcaccggcc acggtcagca
     7021 gggcctccag cgcacgagca ccgctccact gcttgcccac acccacaatc gcttcatggg
     7081 tagcctctgg cagggcagca atcatgtcct gatatttcac ggccacggta cccagcgcgg
     7141 ctgggtgctg gctcagagcc acgatgtgcg cgtgggtgaa gccgtgaccc accagagctt
     7201 catggtgctg ggccacggtg ctgcgcacct ttggcttgat cttctcctgc tgctgctggc
     7261 tgtaacccag ggtacgcaga tccgcgatgt ccatcttgtc atcgtcatcc ttgtaatcga
     7321 tgtcatgatc tttataatca ccgtcatggt ctttgtagtc cagggaatgg attttcgcac
     7381 gcggaactgg cagacgacga gcggagccgg tcaggccacg cagcagcagc ggggtcagaa
     7441 cggaggccat ggtgctagcc agcttgggtc tccctatagt gagtcgtatt aatttcgata
     7501 agccagtaag cagtgggttc tctagttagc cagagagctc tgcttatata gacctcccac
     7561 cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag
     7621 tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga
     7681 aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc
     7741 atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc
     7801 atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta
     7861 cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc
     7921 cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac
     7981 gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta
     8041 acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa
     8101 ctagtcaata atcaatgtca acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa
     8161 cgcctaaccc taagcagatt cttcatgcaa ttgtcggtca agccttgcct tgttgtagct
     8221 taaattttgc tcgcgcacta ctcagcgacc tccaacacac aagcagggag cagatactgg
     8281 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatagggg atcgggagat
     8341 ctcccgatcc gtcgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     8401 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     8461 tgcttcaata atattga
//
