LOCUS       sequence-443585-       24910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Second generation Rosa26 gene targeting vector to induce a 
            Cre-dependent mosaic of cells expressing different membrane 
            localized fluorescent proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 24910)
  AUTHORS   Pontes-Quero S, Heredia L, Casquero-Garcia V, Fernandez-Chacon M, 
            Luo W, Hermoso A, Bansal M, Garcia-Gonzalez I, Sanchez-Munoz MS, 
            Perea JR, Galiana-Simal A, Rodriguez-Arabaolaza I, Del Olmo-Cabrera 
            S, Rocha SF, Criado-Rodriguez LM, Giovinazzo G, Benedito R
  TITLE     Dual ifgMosaic: A Versatile Method for Multispectral and 
            Combinatorial Mosaic Gene-Function Analysis.
  JOURNAL   Cell. 2017 Aug 10;170(4):800-814.e18. doi: 
            10.1016/j.cell.2017.07.031.
  PUBMED    28802047
REFERENCE   2  (bases 1 to 24910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07"; journalName: "Cell"; date: "2017-08-10- 
            10"; volume: "170"; issue: "4"; pages: "800-814.e18"
COMMENT     Sequence Label: iMb2-Control-Mosaic (SO273)
FEATURES             Location/Qualifiers
     source          1..24910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1060..1076
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(4612..4677,4678..4715)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     enhancer        4744..5123
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5125..5403
                     /label=chicken beta-actin promoter
     intron          5404..6420
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6344..6366
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6428..6447
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(6481..6514)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(6522..6555)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(6564..6597)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6611..6620
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6656..6676
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6677..7381
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTAGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTYL"
     polyA_signal    7564..7685
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7697..7730)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      7737..7746
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7872..7874,7875..8585)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7914..7935)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8175..8194)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8522..8543
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8595..8648
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    8705..9293
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(9176..9187)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    9427..9548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(9563..9596)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      9609..9618
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9744..11168
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVL
                     KGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYER
                     SEGRHHLFLYGMDELYK"
     CDS             11178..11231
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    11288..11876
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(11759..11770)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    12010..12131
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(12146..12179)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      12186..12195
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12321..12347
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             12354..12380
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             12387..12413
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             12423..13130
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     misc_feature    13262..13850
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(13733..13744)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    13984..14105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    14143..14176
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        14240..14739
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(14275..14298)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     14672..14691
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        14751..14798
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             14817..15620
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(14880..14899)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     15490..15509
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(15652..15669)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    15658..15882
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    15940..15973
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(15982..16015)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    16021..16054
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    18668..23008
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(23059..23075)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(23059..23075)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(23107..23126)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(23108..23126)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(23136..23153)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(23136..23152)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(23145..23167)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(23293..23748)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(23430..23451)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     23642..23661
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        23775..23879
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(23880..23948,23949..24740)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(24098..24117)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tgggtaccgg gccccccctc gaggtcgacg gtatcgataa
     1081 gcttcacgtg ttcgaaacgc ggtccgccct gcagcaaccg gagggggagg gagaagggag
     1141 cggaaaagtc tccaccggac gcggccatgg ctcggggggg ggggggggca gcggaggagc
     1201 gcttccggcc gacgtctcgt cgctgattgg cttcttttcc tcccgccgtg tgtgaaaaca
     1261 caaatggcgt gttttggttg gcgtaaggcg cctgtcagtt aacggcagcc ggagtgcgca
     1321 gccgccggca gcctcgctct gcccactggg tggggcggga ggtaggtggg gtgaggcgag
     1381 ctggacgtgc gggcgcggtc ggcctctggc ggggcggggg aggggaggga gggtcagcga
     1441 aagtagctcg cgcgcgagcg gccgcccacc ctccccttcc tctgggggag tcgttttacc
     1501 cgccgccggc cgggcctcgt cgtctgattg gctctcgggg cccagaaaac tggcccttgc
     1561 cattggctcg tgttcgtgca agttgagtcc atccgccggc cagcgggggc ggcgaggagg
     1621 cgctcccagg ttccggccct cccctcggcc ccgcgccgca gagtctggcc gcgcgcccct
     1681 gcgcaacgtg gcaggaagcg cgcgctgggg gcggggacgg gcagtagggc tgagcggctg
     1741 cggggcgggt gcaagcacgt ttccgacttg agttgcctca agaggggcgt gctgagccag
     1801 acctccatcg cgcactccgg ggagtggagg gaaggagcga gggctcagtt gggctgtttt
     1861 ggaggcagga agcacttgct ctcccaaagt cgctctgagt tgttatcagt aagggagctg
     1921 cagtggagta ggcggggaga aggccgcacc cttctccgga ggggggaggg gagtgttgca
     1981 atacctttct gggagttctc tgctgcctcc tggcttctga ggaccgccct gggcacatga
     2041 tggacctgtc gacaattcga gctcgctaga gccccatcct cactgactcc gtcctggagt
     2101 tggatgagag ataatggcct tacgttgtgc caggggaggg tcgggctgga tttagcaaga
     2161 tttaccttct ccaaagagcg gtgctgcagt ggcacagctg cccacggagg tgggggggtc
     2221 accgtccctg gaggtgatga agaactgtgg ggatgtggca ctgagggaca tggccagtgg
     2281 gcacggtggg tgggttgggg ttggtcttgg ggatcttgga gggcttttcc agccttcatg
     2341 atttgacgat tgtatgaaca tctacatggc aattctccag ctgcctgtcc cagtcctact
     2401 gacccagctg tatctctcca ggcaagctct tccacccctt ctgcttgcat ccagacacca
     2461 tcaaacatgc aggctcagac acagggacca gcagtgtctg tggccttttt gtgctcctct
     2521 ccatgctggg ttttaacttg ctctttgtcc ttctatccta tcttcttatc cttaaggctg
     2581 ttctgaacgc tgtgacttgg agagtgtccc agagccctca acacctgcat gtcccacgtc
     2641 catgctgtcc tgcacttcct tatccccaag atctgcctct ccgtgatgca ctgaattggc
     2701 aaacatgtgt caccccagac caacaatgtc acagcaaact cccccttgat aggacaaggg
     2761 ggaatggctt tacactgaga caggggaggt ttgggttgga tatgaggagg cagtttttcc
     2821 cccagagggt ggtgacgcac tgaacaggtt gcccaaggag gctgtggatg ccccatccct
     2881 gcaggcattc aaggccaggc tggatgtggc tctgggcagc ctgggctgct ggttgatgac
     2941 cctgcacata gcagggggtt ggatctggat gagcactgtg ctcctttgca acccaggccg
     3001 ttctatgatt ctgtcattct aaatctctct ttcagcctaa agctttttcc ccgtatcccc
     3061 ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc
     3121 accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc
     3181 cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat
     3241 tacatccctg ggggctttgg gggggggctg tccctctagc gagctcggta cccagagccc
     3301 catcctcact gactccgtcc tggagttgga tgagagataa tggccttacg ttgtgccagg
     3361 ggagggtcgg gctggattta gcaagattta ccttctccaa agagcggtgc tgcagtggca
     3421 cagctgccca cggaggtggg ggggtcaccg tccctggagg tgatgaagaa ctgtggggat
     3481 gtggcactga gggacatggc cagtgggcac ggtgggtggg ttggggttgg tcttggggat
     3541 cttggagggc ttttccagcc ttcatgattt gacgattgta tgaacatcta catggcaatt
     3601 ctccagctgc ctgtcccagt cctactgacc cagctgtatc tctccaggca agctcttcca
     3661 ccccttctgc ttgcatccag acaccatcaa acatgcaggc tcagacacag ggaccagcag
     3721 tgtctgtggc ctttttgtgc tcctctccat gctgggtttt aacttgctct ttgtccttct
     3781 atcctatctt cttatcctta aggctgttct gaacgctgtg acttggagag tgtcccagag
     3841 ccctcaacac ctgcatgtcc cacgtccatg ctgtcctgca cttccttatc cccaagatct
     3901 gcctctccgt gatgcactga attggcaaac atgtgtcacc ccagaccaac aatgtcacag
     3961 caaactcccc cttgatagga caagggggaa tggctttaca ctgagacagg ggaggtttgg
     4021 gttggatatg aggaggcagt ttttccccca gagggtggtg acgcactgaa caggttgccc
     4081 aaggaggctg tggatgcccc atccctgcag gcattcaagg ccaggctgga tgtggctctg
     4141 ggcagcctgg gctgctggtt gatgaccctg cacatagcag ggggttggat ctggatgagc
     4201 actgtgctcc tttgcaaccc aggccgttct atgattctgt cattctaaat ctctctttca
     4261 gcctaaagct ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag
     4321 cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg
     4381 ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct
     4441 gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc
     4501 tctaggggta cccggggatc ttaattggcc gtggctagtc gacccagggc ctgggagaat
     4561 cccttccccc tcttccctcg tgatctgcaa ctccagtctt tctagcatct gtagggcgca
     4621 gtagtccagg gtttccttga tgatgtcata cttatcctgt cccttttttt tccacagctc
     4681 gcggttgagg acaaactctt cgcggtcttt ccagtggtta atgcaccttc cttccttcga
     4741 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     4801 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     4861 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     4921 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     4981 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     5041 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     5101 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
     5161 tctccccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     5221 gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg
     5281 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     5341 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     5401 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
     5461 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     5521 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
     5581 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
     5641 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
     5701 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     5761 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     5821 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     5881 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     5941 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     6001 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc
     6061 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     6121 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     6181 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     6241 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     6301 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     6361 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     6421 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcctcgag
     6481 ataacttcgt atagtatacc ttatacgaag ttatcgagct cataacttcg tataggatac
     6541 tttatacgaa gttataactc gagataactt cgtatagcat acattatacg aagttatacc
     6601 ggcagatctc gccaccatgg tgagtgtgat tacagaggct agcttcacca tggctcctaa
     6661 gaagaagagg aaggtgatga gcagcggcgc cctgctgttc cacggcaaga tcccctacgt
     6721 ggtggagatg gagggcaatg tggatggcca caccttcagc atccgcggca agggctacgg
     6781 cgatgccagc gtgggcaagg tggatgccca gttcatctgc accacaggcg atgtgcccgt
     6841 gccctggagc accctggtga ccaccctgac cgcaggcgcc cagtgcttcg ccaagtacgg
     6901 ccccgagctg aaggatttct acaagagctg catgcccgat ggctacgtgc aggagcgcac
     6961 catcaccttc gagggtgatg gcaatttcaa gacccgcgcc gaggtgacct tcgagaatgg
     7021 cagcgtgtac aatcgcgtga agctgaatgg ccagggcttc aagaaggatg gccacgtgct
     7081 gggcaagaat ctggagttca atttcacccc ccactgcctg tacatctggg gcgatcaggc
     7141 caatcacggc ctgaagagcg ccttcaagat ctgccacgag atcaccggca gcaagggcga
     7201 tttcatcgtg gccgatcaca cccagatgaa tacccccatc ggcggcggcc ccgtgcacgt
     7261 gcccgagtac caccacatga gctaccacgt gaagctgagc aaggatgtga ccgatcaccg
     7321 cgataatatg agcctgaagg agaccgtgcg cgccgtggat tgccgcaaga cctacctgtg
     7381 acgcttaatt agctgagctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
     7441 atctggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
     7501 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     7561 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7621 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7681 tcttaaggcg aagcttataa cttcgtatag tataccttat acgaagttat cggcgcgcca
     7741 ccatgggttg ctgtttctcc aagaccgcag cgaagggaga agccgccgcg gagaggcctg
     7801 gggaggcggc tgtggcctcg tcgccttcca aagcgaacgg acaggagaat ggccacgtga
     7861 aggtaggatc cgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     7921 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     7981 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     8041 ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc taccccgacc
     8101 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     8161 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     8221 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     8281 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     8341 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     8401 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     8461 acaaccacta cctgagctac cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc
     8521 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     8581 acaagggcag tggagagggc agaggaagtc tgctaacatg cggtgacgtc gaggagaatc
     8641 ctggcccaga attctgcaga tatccagcac agtggcggcc gctcgagtct aggcgcgccc
     8701 cgctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     8761 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     8821 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     8881 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     8941 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     9001 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     9061 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     9121 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     9181 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     9241 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     9301 ccgtcgagac tgctgcacca gatcataatc agccatacca catttgtaga ggttttactt
     9361 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     9421 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     9481 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9541 gtatcttaag gcgtggagat ctataacttc gtataggata ctttatacga agttatcggt
     9601 accggcgcgc caccatgggt tgctgtttct ccaagaccgc agcgaaggga gaagccgccg
     9661 cggagaggcc tggggaggcg gctgtggcct cgtcgccttc caaagcgaac ggacaggaga
     9721 atggccacgt gaaggtagga tccgtgagca agggagagga ggtcatcaaa gagttcatgc
     9781 gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg
     9841 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc
     9901 ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg
     9961 tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt
    10021 gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc
    10081 tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg
    10141 gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc
    10201 gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact
    10261 acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact
    10321 actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac
    10381 agtacgagcg ctccgagggc cgccaccacc tgttcctggg gcatggcacc ggcagcaccg
    10441 gcagcggcag ctccggcacc gcctcctccg aggacaacaa catggccgtc atcaaagagt
    10501 tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg
    10561 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
    10621 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg
    10681 cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct
    10741 tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact
    10801 cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc
    10861 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
    10921 acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg
    10981 gccgctacct ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg
    11041 gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg
    11101 tggaacagta cgagcgctcc gagggccgcc accacctgtt cctgtacggc atggacgagc
    11161 tgtacaaggg cagtggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga
    11221 atcctggccc agaattctgc agatatccag cacagtggcg gccgctcgag tctaggcgcg
    11281 ccccgctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
    11341 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
    11401 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
    11461 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
    11521 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
    11581 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
    11641 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
    11701 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
    11761 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
    11821 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
    11881 ataccgtcga gactgctgca ccagatcata atcagccata ccacatttgt agaggtttta
    11941 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
    12001 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    12061 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    12121 aatgtatctt aaggcgtgga ctagtataac ttcgtatagc atacattata cgaagttatc
    12181 ggcgcgccac catgggttgc tgtttctcca agaccgcagc gaagggagaa gccgccgcgg
    12241 agaggcctgg ggaggcggct gtggcctcgt cgccttccaa agcgaacgga caggagaatg
    12301 gccacgtgaa ggtaggatcc tacccatacg atgttcctga ctatgcggcc gaatatccct
    12361 atgacgtccc ggactatgca gccgaatatc catacgacgt tccagattac gctgccaaac
    12421 ttatggtgag caagggcgag gagaccacaa tgggcgtaat caagcccgac atgaagatca
    12481 agctgaagat ggagggcaac gtgaatggcc acgccttcgt gatcgagggc gagggcgagg
    12541 gcaagcccta cgacggcacc aacaccatca acctggaggt gaaggaggga gcccccctgc
    12601 ccttctccta cgacattctg accaccgcgt tcgcctacgg caacagggcc ttcaccaagt
    12661 accccgacga catccccaac tacttcaagc agtccttccc cgagggctac tcttgggagc
    12721 gcaccatgac cttcgaggac aagggcatcg tgaaggtgaa gtccgacatc tccatggagg
    12781 aggactcctt catctacgag atacacctca agggcgagaa cttccccccc aacggccccg
    12841 tgatgcagaa gaagaccacc ggctgggacg cctccaccga gaggatgtac gtgcgcgacg
    12901 gcgtgctgaa gggcgacgtc aagcacaagc tgctgctgga gggcggcggc caccaccgcg
    12961 ttgacttcaa gaccatctac agggccaaga aggcggtgaa gctgcccgac tatcactttg
    13021 tggaccaccg catcgagatc ctgaaccacg acaaggacta caacaaggtg accgtttacg
    13081 agagcgccgt ggcccgcaac tccaccgacg gcatggacga gctgtataag agtgcacgcg
    13141 tgagtaagct gaaccctcct gatgagagtg gccccggctg catgagctgc aagtgtgtgc
    13201 tctccctgta caagtgagaa ttctgcagat atccagcaca gtctcgaggg cgcgccccgc
    13261 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
    13321 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
    13381 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
    13441 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
    13501 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
    13561 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
    13621 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
    13681 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
    13741 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
    13801 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
    13861 tcgagactgc tgcaccagat cataatcagc cataccacat ttgtagaggt tttacttgct
    13921 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
    13981 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    14041 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    14101 tcttaaggcg tggcggccgc agcataggga tccccgaatt ccgaagttcc tattctctag
    14161 aaagtatagg aacttcaggt ctgaagagga gtttacgtcc agccaagcta gcttggctgc
    14221 aggtcgtcga aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
    14281 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
    14341 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
    14401 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
    14461 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
    14521 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
    14581 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
    14641 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
    14701 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagcct gttgacaatt
    14761 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
    14821 gatcggccat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
    14881 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
    14941 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
    15001 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
    15061 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
    15121 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
    15181 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
    15241 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
    15301 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
    15361 ccgacggcga tgatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
    15421 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
    15481 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
    15541 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
    15601 ttcttgacga gttcttctga ggggatcaat tctctagagc tcgctgatca gcctcgactg
    15661 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
    15721 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
    15781 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
    15841 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
    15901 ccagctgggg ctcgactaga gcttgcggaa cccttcgttg aagttcctat tctctagaaa
    15961 gtataggaac ttcagtcgac tgaagttcct atactttcta gagaatagga acttcaacgt
    16021 gaagttccta ttctctagaa agtataggaa cttcagtcga cgtgctccaa ggagagtttg
    16081 ctggcaagat cttcggggtc gatgctaaca cagggaatgt gcttgcctat gagaggctag
    16141 accgggagaa agtctctgag tatcgtcgac aattcgagct cgctagagcc ccatcctcac
    16201 tgactccgtc ctggagttgg atgagagata atggccttac gttgtgccag gggagggtcg
    16261 ggctggattt agcaagattt accttctcca aagagcggtg ctgcagtggc acagctgccc
    16321 acggaggtgg gggggtcacc gtccctggag gtgatgaaga actgtgggga tgtggcactg
    16381 agggacatgg ccagtgggca cggtgggtgg gttggggttg gtcttgggga tcttggaggg
    16441 cttttccagc cttcatgatt tgacgattgt atgaacatct acatggcaat tctccagctg
    16501 cctgtcccag tcctactgac ccagctgtat ctctccaggc aagctcttcc accccttctg
    16561 cttgcatcca gacaccatca aacatgcagg ctcagacaca gggaccagca gtgtctgtgg
    16621 cctttttgtg ctcctctcca tgctgggttt taacttgctc tttgtccttc tatcctatct
    16681 tcttatcctt aaggctgttc tgaacgctgt gacttggaga gtgtcccaga gccctcaaca
    16741 cctgcatgtc ccacgtccat gctgtcctgc acttccttat ccccaagatc tgcctctccg
    16801 tgatgcactg aattggcaaa catgtgtcac cccagaccaa caatgtcaca gcaaactccc
    16861 ccttgatagg acaaggggga atggctttac actgagacag gggaggtttg ggttggatat
    16921 gaggaggcag tttttccccc agagggtggt gacgcactga acaggttgcc caaggaggct
    16981 gtggatgccc catccctgca ggcattcaag gccaggctgg atgtggctct gggcagcctg
    17041 ggctgctggt tgatgaccct gcacatagca gggggttgga tctggatgag cactgtgctc
    17101 ctttgcaacc caggccgttc tatgattctg tcattctaaa tctctctttc agcctaaagc
    17161 tttttccccg tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag
    17221 gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg
    17281 ggatgcgggg ggagcgccgg accggagcgg agccccgggc ggctcgctgc tgccccctag
    17341 cgggggaggg acgtaattac atccctgggg gctttggggg ggggctgtcc ctctagcgag
    17401 ctcggtaccc agagccccat cctcactgac tccgtcctgg agttggatga gagataatgg
    17461 ccttacgttg tgccagggga gggtcgggct ggatttagca agatttacct tctccaaaga
    17521 gcggtgctgc agtggcacag ctgcccacgg aggtgggggg gtcaccgtcc ctggaggtga
    17581 tgaagaactg tggggatgtg gcactgaggg acatggccag tgggcacggt gggtgggttg
    17641 gggttggtct tggggatctt ggagggcttt tccagccttc atgatttgac gattgtatga
    17701 acatctacat ggcaattctc cagctgcctg tcccagtcct actgacccag ctgtatctct
    17761 ccaggcaagc tcttccaccc cttctgcttg catccagaca ccatcaaaca tgcaggctca
    17821 gacacaggga ccagcagtgt ctgtggcctt tttgtgctcc tctccatgct gggttttaac
    17881 ttgctctttg tccttctatc ctatcttctt atccttaagg ctgttctgaa cgctgtgact
    17941 tggagagtgt cccagagccc tcaacacctg catgtcccac gtccatgctg tcctgcactt
    18001 ccttatcccc aagatctgcc tctccgtgat gcactgaatt ggcaaacatg tgtcacccca
    18061 gaccaacaat gtcacagcaa actccccctt gataggacaa gggggaatgg ctttacactg
    18121 agacagggga ggtttgggtt ggatatgagg aggcagtttt tcccccagag ggtggtgacg
    18181 cactgaacag gttgcccaag gaggctgtgg atgccccatc cctgcaggca ttcaaggcca
    18241 ggctggatgt ggctctgggc agcctgggct gctggttgat gaccctgcac atagcagggg
    18301 gttggatctg gatgagcact gtgctccttt gcaacccagg ccgttctatg attctgtcat
    18361 tctaaatctc tctttcagcc taaagctttt tccccgtatc cccccaggtg tctgcaggct
    18421 caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg
    18481 gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc
    18541 ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt
    18601 tggggggggg ctgtccctct aggggtaccc ggggatctta attaacggcg gcggagtatc
    18661 gaattcgcta gaagatgggc gggagtcttc tgggcaggct taaaggctaa cctggtgtgt
    18721 gggcgttgtc ctgcagggga attgaacagg tgtaaaattg gagggacaag acttcccaca
    18781 gattttcggt tttgtcggga agttttttaa taggggcaaa taaggaaaat gggaggatag
    18841 gtagtcatct ggggttttat gcagcaaaac tacaggttat tattgcttgt gatccgcctc
    18901 ggagtatttt ccatcgaggt agattaaaga catgctcacc cgagttttat actctcctgc
    18961 ttgagatcct tactacagta tgaaattaca gtgtcgcgag ttagactatg taagcagaat
    19021 tttaatcatt tttaaagagc ccagtacttc atatccattt ctcccgctcc ttctgcagcc
    19081 ttatcaaaag gtattttaga acactcattt tagccccatt ttcatttatt atactggctt
    19141 atccaacccc tagacagagc attggcattt tccctttcct gatcttagaa gtctgatgac
    19201 tcatgaaacc agacagatta gttacataca ccacaaatcg aggctgtagc tggggcctca
    19261 acactgcagt tcttttataa ctccttagta cactttttgt tgatcctttg ccttgatcct
    19321 taattttcag tgtctatcac ctctcccgtc aggtggtgtt ccacatttgg gcctattctc
    19381 agtccaggga gttttacaac aatagatgta ttgagaatcc aacctaaagc ttaactttcc
    19441 actcccatga atgcctctct cctttttctc catttataaa ctgagctatt aaccattaat
    19501 ggtttccagg tggatgtctc ctcccccaat attacctgat gtatcttaca tattgccagg
    19561 ctgatatttt aagacattaa aaggtatatt tcattattga gccacatggt attgattact
    19621 gcttactaaa attttgtcat tgtacacatc tgtaaaaggt ggttcctttt ggaatgcaaa
    19681 gttcaggtgt ttgttgtctt tcctgaccta aggtcttgtg agcttgtatt ttttctattt
    19741 aagcagtgct ttctcttgga ctggcttgac tcatggcatt ctacacgtta ttgctggtct
    19801 aaatgtgatt ttgccaagct tcttcaggac ctataatttt gcttgacttg tagccaaaca
    19861 caagtaaaat gattaagcaa caaatgtatt tgtgaagctt ggtttttagg ttgttgtgtt
    19921 gtgtgtgctt gtgctctata ataatactat ccaggggctg gagaggtggc tcggagttca
    19981 agagcacaga ctgctcttcc agaagtcctg agttcaattc ccagcaacca catggtggct
    20041 cacaaccatc tgtaatggga tctgatgccc tcttctggtg tgtctgaaga ccacaagtgt
    20101 attcacatta aataaataaa tcctccttct tcttcttttt ttttttttaa agagaatact
    20161 gtctccagta gaatttactg aagtaatgaa atactttgtg tttgttccaa tatggtagcc
    20221 aataatcaaa ttactcttta agcactggaa atgttaccaa ggaactaatt tttatttgaa
    20281 gtgtaactgt ggacagagga gccataactg cagacttgtg ggatacagaa gaccaatgca
    20341 gactttaatg tcttttctct tacactaagc aataaagaaa taaaaattga acttctagta
    20401 tcctatttgt ttaaactgct agctttactt aacttttgtg cttcatctat acaaagctga
    20461 aagctaagtc tgcagccatt actaaacatg aaagcaagta atgataattt tggatttcaa
    20521 aaatgtaggg ccagagttta gccagccagt ggtggtgctt gcctttatgc ctttaatccc
    20581 agcactctgg aggcagagac aggcagatct ctgagtttga gcccagcctg gtctacacat
    20641 caagttctat ctaggatagc caggaataca cacagaaacc ctgttgggga ggggggctct
    20701 gagatttcat aaaattataa ttgaagcatt ccctaatgag ccactatgga tgtggctaaa
    20761 tccgtctacc tttctgatga gatttgggta ttattttttc tgtctctgct gttggttggg
    20821 tcttttgaca ctgtgggctt tctttaaagc ctccttcctg ccatgtggtc tcttgtttgc
    20881 tactaacttc ccatggctta aatggcatgg ctttttgcct tctaagggca gctgctgaga
    20941 tttgcagcct gatttccagg gtggggttgg gaaatctttc aaacactaaa attgtccttt
    21001 aatttttttt ttaaaaaatg ggttatataa taaacctcat aaaatagtta tgaggagtga
    21061 ggtggactaa tattaaatga gtccctcccc tataaaagag ctattaaggc tttttgtctt
    21121 atacttaact ttttttttaa atgtggtatc tttagaacca agggtcttag agttttagta
    21181 tacagaaact gttgcatcgc ttaatcagat tttctagttt caaatccaga gaatccaaat
    21241 tcttcacagc caaagtcaaa ttaagaattt ctgactttta atgttaattt gcttactgtg
    21301 aatataaaaa tgatagcttt tcctgaggca gggtctcact atgtatctct gcctgatctg
    21361 caacaagata tgtagactaa agttctgcct gcttttgtct cctgaatact aaggttaaaa
    21421 tgtagtaata cttttggaac ttgcaggtca gattctttta taggggacac actaagggag
    21481 cttgggtgat agttggtaaa atgtgtttca agtgatgaaa acttgaatta ttatcaccgc
    21541 aacctacttt ttaaaaaaaa aagccaggcc tgttagagca tgcttaaggg atccctagga
    21601 cttgctgagc acacaagagt agttacttgg caggctcctg gtgagagcat atttcaaaaa
    21661 acaaggcaga caaccaagaa actacagtta aggttacctg tctttaaacc atctgcatat
    21721 acacagggat attaaaatat tccaaataat atttcattca agttttcccc catcaaattg
    21781 ggacatggat ttctccggtg aataggcaga gttggaaact aaacaaatgt tggttttgtg
    21841 atttgtgaaa ttgttttcaa gtgatagtta aagcccatga gatacagaac aaagctgcta
    21901 tttcgaggtc tcttggttta tactcagaag cacttctttg ggtttccctg cactatcctg
    21961 atcatgtgct aggcctacct taggctgatt gttgttcaaa taaacttaag tttcctgtca
    22021 ggtgatgtca tatgatttca tatatcaagg caaaacatgt tatatatgtt aaacatttgt
    22081 acttaatgtg aaagttaggt ctttgtgggt ttgattttta attttcaaaa cctgagctaa
    22141 ataagtcatt tttacatgtc ttacatttgg tggaattgta taattgtggt ttgcaggcaa
    22201 gactctctga cctagtaacc ctacctatag agcactttgc tgggtcacaa gtctaggagt
    22261 caagcatttc accttgaagt tgagacgttt tgttagtgta tactagttta tatgttggag
    22321 gacatgttta tccagaagat attcaggact atttttgact gggctaagga attgattctg
    22381 attagcactg ttagtgagca ttgagtggcc tttaggcttg aattggagtc acttgtatat
    22441 ctcaaataat gctggccttt tttaaaaagc ccttgttctt tatcaccctg ttttctacat
    22501 aatttttgtt caaagaaata cttgtttgga tctccttttg acaacaatag catgttttca
    22561 agccatattt tttttccttt tttttttttt tttttggttt ttcgagacag ggtttctctg
    22621 tatagccctg gctgtcctgg aactcacttt gtagaccagg ctggcctcga actcagaaat
    22681 ccgcctgcct ctgcctcctg agtgccggga ttaaaggcgt gcaccaccac gcctggctaa
    22741 gttggatatt ttgttatata actataacca atactaactc cactgggtgg atttttaatt
    22801 cagtcagtag tcttaagtgg tctttattgg cccttcatta aaatctactg ttcactctaa
    22861 cagaggctgt tggtactagt ggcacttaag caacttccta cggatatact agcagattaa
    22921 gggtcaggga tagaaactag tctagcgttt tgtataccta ccagctttat actaccttgt
    22981 tctgatagaa atatttcagg acatctagct tatcgatacc gtcgacgttc gaacacgtgg
    23041 aattcctgca gcccggggga tccactagtt ctagagcggc cgccaccgcg gtggagctcc
    23101 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
    23161 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
    23221 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
    23281 aatggcgaat ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
    23341 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
    23401 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
    23461 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
    23521 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
    23581 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
    23641 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
    23701 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
    23761 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    23821 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    23881 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    23941 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    24001 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    24061 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    24121 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    24181 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    24241 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    24301 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    24361 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    24421 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    24481 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    24541 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    24601 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    24661 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    24721 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    24781 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    24841 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    24901 aaggatcttc
//
