LOCUS       sequence-443588-        8730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expresses truncated human FHF (FTS, StrepII-HOOK3[571-718] and 
            FHIP1B) in a pBIG1a vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   Abid Ali F, Zwetsloot AJ, Stone CE, Morgan TE, Wademan RF, Carter 
            AP, Straube A
  TITLE     KIF1C activates and extends dynein movement through the FHF cargo 
            adapter.
  JOURNAL   Nat Struct Mol Biol. 2025 Apr;32(4):756-766. doi: 
            10.1038/s41594-024-01418-z. Epub 2025 Jan 2.
  PUBMED    39747486
REFERENCE   2  (bases 1 to 8730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-024-01418-z"; journalName: "Nat Struct Mol Biol"; 
            date: "2025-04"; volume: "32"; issue: "4"; pages: "756-766"
COMMENT     Sequence Label: pBIG1a_StrepII-Hook3[571-718]_FTS_FHIP1B
FEATURES             Location/Qualifiers
     source          1..8730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     679..698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  913..1136
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1203..1736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1925..1930,1931..1947,1948..1953))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2024..2057)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2103..2122)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2147..2195
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        2290..2381
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             2434..2457
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2494..2517
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2518..2541
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    3145..3279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3435..3526
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    6651..6785
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6941..7032
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    8117..8251
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  8565..8730
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
     1741 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     1801 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     1861 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     1921 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     1981 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat
     2041 acattatacg aagttatctg ggtttaaacg ctctatggtc taaagttttt atcgaggcta
     2101 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     2161 cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     2221 ctggcactct gtcaagtgaa cgctctatgg tctaaagatt taaatcgacc tactccggaa
     2281 tattaataga tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt
     2341 actgttttcg taacagtttt gtaataaaaa aacctataaa tattccggat tattcatacc
     2401 gtcccaccat cgggcgcgga tccctcgagc atgtggtcac atccgcagtt tgaaaaaggt
     2461 agcgcaggta gtgcagcagg tagcggtgca ggttggagcc atcctcagtt tgagaaactg
     2521 gaagttctgt ttcagggtcc ggatctcgag ccaagattta acaacagctc cttaaaaatt
     2581 gaagaattac aagaagcttt acgaaagaaa gaggaagaaa tgaagcaaat ggaagaacga
     2641 tacaaaaaat acttagagaa agccaaaagt gtcatccgta ctttagatcc taaacagaat
     2701 caaggagcag caccagaaat acaagctctt aaaaatcagc tccaggaacg agaccgactg
     2761 ttccactcat tagagaaaga atatgagaaa acaaagagtc agagagagat ggaagagaaa
     2821 tatattgtta gtgcctggta caatatggga atgaccctgc ataaaaaggc agctgaagat
     2881 agactggcaa gcacaggctc agggcagtca tttctggcga ggcagaggca agcgaccagc
     2941 agcagaagat catacccagg ccacgtgcag ccggccacag caaggtaagg tacctctaga
     3001 gcctgcagtc tcgacaagct tgtcgagaag tactagagga tcataatcag ccataccaca
     3061 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     3121 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     3181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3241 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct tgagcctaga
     3301 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     3361 ataactatca taaccattta aataaactgg atactattgc acgtttaaat cgacctactc
     3421 cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt
     3481 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc
     3541 ataccgtccc accatcgggc gcggatccct cgagcatgga aagaatgaat tggctctccc
     3601 gtctcgcctc taggggccca gggcacagga ttccccaagg ggcgaatctc caaacccctg
     3661 ttatggccga cccagaaacc tgcctcatgg tattcaagaa ccattggtcc caagtggtga
     3721 gaatactgga acgtcaaggg ccccgtgccg ccccaggtgg ggcggacgac ctcagtgccg
     3781 tcagaaatca tacatatcaa atgctgaccc ttctggctga agatagggcc gtgccatccg
     3841 cccccacagg tccaggcccg ctgttggaat tcgctctcca cgaggatctc ctgactcggg
     3901 tcctgacttg gcaattgcag tgggacgagc ttggggacgg cgttgaagag cggagagccg
     3961 agcagctgaa gttgttcgag atgcttgtca gtgaggcacg acaacccctt ctcagacatg
     4021 gcccggtccg cgaagcgctt ctgaccctgc ttgacgcttg cggccgcccc gttccttcaa
     4081 gtcctgcact tgatgagggg ctcgtgctct tgctctccca actttgcgtg tgtgtggcac
     4141 aagaacccag tctgttggag ttctttcttc agccaccacc cgaaccaggc gcggctcccc
     4201 gattgcttct gttcagccgg ttggtgccgt ttgttcatct agagggcaca ttgggccaac
     4261 aagcccgaga tgcgctgctc ttgctgatgg cgcttagtgc tggctcacca actgttggaa
     4321 ggtatattgc agatcacagc tacttttgcc ccgtactggc tactgggttg agcgcgctct
     4381 atagctcatt gccgcgtaaa attgaggtcc ccggtgatga ttggcattgt ctgcgtagag
     4441 aagattggtt gggtgtaccc gccctggcct tgttcatgag ctcactggaa ttctgcaacg
     4501 cggttataca agtggcgcac ccattggttc agaagcaact ggttgattac atccataatg
     4561 gttttctcgt acctgtcatg ggcccagcct tgcataagac cagtgtggaa gaaatgatcg
     4621 caagtacagc ttatttggaa ttgttcttgc gctccatctc agagccagcc ctgctcagaa
     4681 cctttctccg ctttctcctg ctgcaccgac atgacactca tacaatcctc gatacgctgg
     4741 tggctagaat tggatccaat agccgcttgt gcatggtgag cttgtctctc tttagaacac
     4801 tgcttaattt gtcctgcgag gatgtcctgt tgcaactggt gctgaggtat ttggttccat
     4861 gtaaccacgt tatgttgtcc cagaaacccg ctgttcgtga tgtggacctt tatggcagag
     4921 ccgcagataa gttcctcagt ctgattcccc gttgctgccg gcatcatgcc ccctcacccc
     4981 cgcgacccga acatgcgtca tgggcccgag gccctgggag ccccagcgtt gactctagca
     5041 gcgtgactac cgtccctcgg cccagcactc caagccgtct ggcccttttc cttcgacaac
     5101 aaagcctggg cggatctgaa agtcccgggc ccgccccatg ctcacccggt ctctccgcat
     5161 ctccggcatc tagtccggga cgtcgaccca cgccagctga ggagcctggg gagctcgagg
     5221 ataattatct cgagtacttg agggaagcta gacggggcgt cgatcgatgt gttcgcgcct
     5281 gccgcacttg gtctgcgcct tatgacggtg aacggccgtc cccagaacct tctcccttcg
     5341 ggagcaggac caagaaacgc tccctgctcc cagaagaaga ccggaataac gtgggagagg
     5401 gcgaagaaga ggaacttggg cgcagaggtc gcgcaggcgg cgcgggtgag ggacccggtc
     5461 atctgccacc accacaactt aacggggtgc caggcagttg gcccgaaggc gctaagaagg
     5521 taagactggt gccaaaagaa ggagccggag agcttcttga aggaatctcc gagggaatgg
     5581 caggattgga aggcttcggt caagaactta gagagctcga ggttgcactg tctaatggtg
     5641 gaacggggag tgaaagcccc ctggaacccc cgctgccttt ggaagaagag gaagcctatg
     5701 agagcttcac atgtccccct gagccgccag ggcccttcct gtcctcacca ctgcgcacct
     5761 tgaatcaact tccttctcag ccatttactg ggccctttat ggctgtattg tttgctaagc
     5821 tcgagaatat gctccaaaac agcgtctacg tcaattttct cctcacaggt ctggtggcac
     5881 aactggcctg ccacccacag cctctgctgc gaagcttctt gctcaatact aacatggtgt
     5941 tccagcctag cgtcaagagt ctgttgcaag tgctgggctc agtgaagaat aagatagaaa
     6001 actttgctgc cagccaggaa gatttcccag cccttctgag taaagccaag aaatatctta
     6061 ttgcacgtgg caaactggac tgggctgaag gccccgcggc cgggccggct ccgaggcgtt
     6121 ccgacccact cgtgaagtct aggcggccgt cactcggaga gctgctcctg cgacacgctc
     6181 attctcccac cagggctaga caagctgcgc agctcgtgct gcaaccggga agggatggtg
     6241 caggcctcgg cctcagcggc gggtcccccg gagcctcaac ccctgtcctt ctcacccgcg
     6301 gcggagcgcc cgaacggcaa ggggaagccc tcagagtaaa gaatgctgtc tactgcgcag
     6361 tgatatttcc agagttcttg aaagagctcg ccgctatttc acaagcacac gcagtgactt
     6421 ccccatttct ccttgagacc tctgaagaag ggtccggccc gcttatatcc ggttgtggac
     6481 cactcaaccc ttaaggtacc tctagagcct gcagtctcga caagcttgtc gagaagtact
     6541 agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     6601 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     6661 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6721 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6781 ggatctgatc actgcttgag cctagaagat ccggctgcta acaaagcccg aaaggaagct
     6841 gagttggctg ctgccaccgc tgagcaataa ctatcataac catttaaata aacctaatga
     6901 tgcctgatgt ttaaatcgac ctactccgga atattaatag atcatggaga taattaaaat
     6961 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     7021 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atccctcgag
     7081 catgaaccct ttctggagta tgagcactag tagtgtccgt aagcgaagtg agggcgaaga
     7141 aaagacgctg actggggacg tgaaaacgtc cccaccccgc acagccccaa agaagcagct
     7201 cccaagcata ccaaagaatg ccctgccaat taccaaaccc acatcccctg cacctgccgc
     7261 acagtctact aacggtacac acgcctctta cggaccattt tatcttgagt acagccttct
     7321 ggctgaattt accctcgtgg taaagcaaaa gctgcctggc gtctacgtgc agccctcata
     7381 caggagcgcc ttgatgtggt tcggagttat ctttatccga catggtctct atcaagatgg
     7441 cgttttcaaa ttcactgtgt atattcctga taattatccc gacggagact gccctcggct
     7501 cgttttcgat attcctgtat tccatcccct tgtcgacccc acatctggtg aactggacgt
     7561 gaaaagggcc ttcgctaagt ggagacggaa ccataatcac atctggcaag tcttgatgta
     7621 tgctcgtaga gtcttttaca aaatcgatac cgcctctccg ttgaacccgg aagccgccgt
     7681 actttacgaa aaggacattc aactgttcaa gagtaaggta gtggatagtg tcaaagtgtg
     7741 tactgcccgc cttttcgacc agcccaaaat tgaggacccg tacgccatct ctttcagtcc
     7801 ctggaaccct agtgtccacg acgaagctcg cgaaaagatg ctcacccaga agaagccaga
     7861 ggaacagcac aataagtctg tccacgtagc agggctgtct tgggtcaagc ctggatctgt
     7921 gcagccattt tccaaggaag aaaagacggt ggctacttaa ggtacctcta gagcctgcag
     7981 tctcgacaag cttgtcgaga agtactagag gatcataatc agccatacca catttgtaga
     8041 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     8101 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     8161 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8221 actcatcaat gtatcttatc atgtctggat ctgatcactg cttgagccta gaagatccgg
     8281 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactat
     8341 cataaccatt taaataaatc tatatctcaa tcggggttag tcgatacccc accgagaccc
     8401 cattgggacc aatacgcccg cgtttcttcc ttttccccac cccaaccccc aagttcgggt
     8461 gaaggcccag ggctcgcagc caacgtcggg gcggcaagcc ctgccatagc cactacgggt
     8521 taattaaaaa ctatatctca atcggggttt aaacaggaga tccgaaccag ataagtgaaa
     8581 tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca
     8641 gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct
     8701 cccagttccc aactattttg tccgcccaca
//
