LOCUS       sequence-443589-        9822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expresses full-length human FHF (FTS, StrepII-HOOK2 and FHIP2A) 
            in a pBIG1a vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Abid Ali F, Zwetsloot AJ, Stone CE, Morgan TE, Wademan RF, Carter 
            AP, Straube A
  TITLE     KIF1C activates and extends dynein movement through the FHF cargo 
            adapter.
  JOURNAL   Nat Struct Mol Biol. 2025 Apr;32(4):756-766. doi: 
            10.1038/s41594-024-01418-z. Epub 2025 Jan 2.
  PUBMED    39747486
REFERENCE   2  (bases 1 to 9822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-024-01418-z"; journalName: "Nat Struct Mol Biol"; 
            date: "2025-04"; volume: "32"; issue: "4"; pages: "756-766"
COMMENT     Sequence Label: pBIG1a_StrepII-Hook2_FTS_FHIP2A
FEATURES             Location/Qualifiers
     source          1..9822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     679..698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  913..1136
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1203..1736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1925..1930,1931..1947,1948..1953))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2024..2057)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2103..2122)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2147..2195
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        2290..2381
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             2434..2457
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2494..2517
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2518..2541
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    4858..4992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5148..5239
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    6324..6458
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6614..6705
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    9209..9343
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  9657..9822
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
     1741 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     1801 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     1861 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     1921 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     1981 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat
     2041 acattatacg aagttatctg ggtttaaacg ctctatggtc taaagttttt atcgaggcta
     2101 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     2161 cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     2221 ctggcactct gtcaagtgaa cgctctatgg tctaaagatt taaatcgacc tactccggaa
     2281 tattaataga tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt
     2341 actgttttcg taacagtttt gtaataaaaa aacctataaa tattccggat tattcatacc
     2401 gtcccaccat cgggcgcgga tccctcgagc atgtggtcac atccgcagtt tgaaaaaggt
     2461 agcgcaggta gtgcagcagg tagcggtgca ggttggagcc atcctcagtt tgagaaactg
     2521 gaagttctgt ttcagggtcc gatgagcgtg gacaaagcag aactgtgcgg atcccttttg
     2581 acttggttgc aaacgttcca cgtcccttca ccatgcgctt ctccccagga tttgtcttca
     2641 ggccttgcag tagcttacgt actgaatcag attgatccaa gctggtttaa cgaggcctgg
     2701 ttgcaaggca tatctgaaga tcctggcccg aattggaaac tgaaggttag taatttgaaa
     2761 atggtgctta ggtcgctggt tgaatattct caggacgtgc tcgcgcatcc cgtatctgag
     2821 gagcatttgc cagacgtctc cttgatcgga gagttctcag atccggccga actcggaaag
     2881 ctcctgcagc tggtattggg ttgcgctatc agctgtgaga agaagcagga tcacattcaa
     2941 aggataatga cgctggaaga aagcgttcag cacgtagtga tggaagctat ccaggaattg
     3001 atgacgaagg atacacccga ttccctctct ccggaaacat acggcaattt tgattcgcaa
     3061 tcgaggagat attacttctt gagcgaagag gctgaggagg gagatgagct ccaacagagg
     3121 tgtttggatc tggaacgtca gctgatgctg ctctcagagg agaaacagtc ccttgcacag
     3181 gaaaatgccg gtctgagaga aaggatgggc cgtccggagg gtgaaggcac cccaggtctg
     3241 accgccaaaa aacttttgct tcttcagtct caattggagc aacttcagga ggaaaatttc
     3301 cgcctcgaaa gcggtcgcga ggacgagcgc ctgagatgtg ccgagctcga gagggaggtt
     3361 gcggaattgc aacacagaaa tcaagccttg acttctttgg ctcaagaagc acaagcactc
     3421 aaagatgaaa tggacgagct taggcaatct agcgagcgcg ccggtcagct tgaagcgaca
     3481 cttacttcgt gtaggagacg tcttggagaa cttagagagt tgaggcgtca ggttcgccag
     3541 ttggaagaaa ggaacgcggg acacgcggag agaacaagac aattggagga tgaacttagg
     3601 cgtgctggtt cgttgagagc gcaactcgag gctcagcgca gacaagtaca ggagctccag
     3661 ggccaaaggc aggaagaggc gatgaaggcg gagaaatggc tctttgaatg taggaacttg
     3721 gaagagaaat atgagtctgt gaccaaggaa aaagaaagac ttctcgctga gagggactct
     3781 cttagagaag caaatgagga acttaggtgc gcccaacttc aacctcgcgg cctcacccaa
     3841 gcggatcctt ctctggatcc gacgagcaca ccagtagata atcttgccgc agaaatcctt
     3901 ccggccgagc ttcgcgagac gcttcttagg cttcaattgg agaataagcg cctctgtagg
     3961 caagaagcgg ctgatcgcga acgtcaagaa gaactccagc gccatttgga agacgcgaat
     4021 agggcgaggc acggattgga gactcagcat aggctgaacc agcagcaact ctcggagctt
     4081 agagcacagg tggaggactt gcagaaagcg ctccaggagc aaggtggcaa aacagaggac
     4141 gccatctcca tccttttgaa gcgtaagctt gaggaacacc tgcagaagct tcatgaggcc
     4201 gacttggaac tgcagaggaa acgcgaatat atcgaagaac tggagccccc caccgactct
     4261 tccactgcca ggaggataga agagctccaa cataacctcc agaaaaaaga cgctgatctt
     4321 agggcgatgg aggaacgtta ccgcagatat gtggataagg cgcgcatggt catgcagact
     4381 atggaaccga agcagagacc agccgctgga gctccccctg agctccactc cctgcgtaca
     4441 caattgagag aaagagatgt gcgcataagg cacctcgaaa tggatttcga aaaaagccgt
     4501 tcacaacgtg aacaagaaga aaaactcctg ataagcgcgt ggtataacat gggtatggct
     4561 ttgcagcaac gcgcaggaga agaaagagcc cctgctcatg cgcaatcgtt ccttgcgcaa
     4621 cagcgcctgg cgaccaattc taggaggggc cctttgggtc gtctcgcctc ccttaacctt
     4681 aggcctaccg acaaacatta aggtacctct agagcctgca gtctcgacaa gcttgtcgag
     4741 aagtactaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     4801 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     4861 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4921 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     4981 catgtctgga tctgatcact gcttgagcct agaagatccg gctgctaaca aagcccgaaa
     5041 ggaagctgag ttggctgctg ccaccgctga gcaataacta tcataaccat ttaaataaac
     5101 tggatactat tgcacgttta aatcgaccta ctccggaata ttaatagatc atggagataa
     5161 ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt
     5221 aataaaaaaa cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc
     5281 cctcgagcat gaaccctttc tggagtatga gcactagtag tgtccgtaag cgaagtgagg
     5341 gcgaagaaaa gacgctgact ggggacgtga aaacgtcccc accccgcaca gccccaaaga
     5401 agcagctccc aagcatacca aagaatgccc tgccaattac caaacccaca tcccctgcac
     5461 ctgccgcaca gtctactaac ggtacacacg cctcttacgg accattttat cttgagtaca
     5521 gccttctggc tgaatttacc ctcgtggtaa agcaaaagct gcctggcgtc tacgtgcagc
     5581 cctcatacag gagcgccttg atgtggttcg gagttatctt tatccgacat ggtctctatc
     5641 aagatggcgt tttcaaattc actgtgtata ttcctgataa ttatcccgac ggagactgcc
     5701 ctcggctcgt tttcgatatt cctgtattcc atccccttgt cgaccccaca tctggtgaac
     5761 tggacgtgaa aagggccttc gctaagtgga gacggaacca taatcacatc tggcaagtct
     5821 tgatgtatgc tcgtagagtc ttttacaaaa tcgataccgc ctctccgttg aacccggaag
     5881 ccgccgtact ttacgaaaag gacattcaac tgttcaagag taaggtagtg gatagtgtca
     5941 aagtgtgtac tgcccgcctt ttcgaccagc ccaaaattga ggacccgtac gccatctctt
     6001 tcagtccctg gaaccctagt gtccacgacg aagctcgcga aaagatgctc acccagaaga
     6061 agccagagga acagcacaat aagtctgtcc acgtagcagg gctgtcttgg gtcaagcctg
     6121 gatctgtgca gccattttcc aaggaagaaa agacggtggc tacttaaggt acctctagag
     6181 cctgcagtct cgacaagctt gtcgagaagt actagaggat cataatcagc cataccacat
     6241 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     6301 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     6361 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6421 tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt gagcctagaa
     6481 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     6541 taactatcat aaccatttaa ataaacctaa tgatgcctga tgtttaaatc gacctactcc
     6601 ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta
     6661 ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca
     6721 taccgtccca ccatcgggcg cggatccctc gagcatgttc tctaagttca cttctattct
     6781 gcaacacgct gttgaggctc tcgctccaag cctgcctctc caggaggatt tcgtctatca
     6841 ctggaaggcc atcactcact actacatcga gacttccgac gacaaagcac cagtcaccga
     6901 caccaacatc ccatctcacc tggaacaaat gctggatatc ctggttcagg aggagaatga
     6961 gagggagtct ggagagacag gtccctgtat ggagtacctc ctgcaccaca agattctcga
     7021 aacactctat accctgggaa aggctgattg tccaccaggt atgaagcagc aggtcctcgt
     7081 gttctatacc aaactgctcg gcagaatcag gcaaccactg ctgccacaca tcaacgtcca
     7141 tagacccgtt cagaagctca ttcgcctctg tggtgaagtt ctggccactc ctactgagaa
     7201 tgaagagatc cagttcctct gtatcgtttg cgctaagctc aaacaggacc cttacctggt
     7261 gaacttcttc ctggagaaca agatgaagtc cctcgcaagc aagggtgtcc ctaacgttat
     7321 ctccgaggac accctgaagg gtcaagatag cctcagcaca gacacaggtc aatctcgcca
     7381 gcctgaggaa ctcagcggag ctaccggaat ggagcaaaca gaactggagg acgaaccacc
     7441 tcatcaaatg gaccacctgt ccacctctct cgacaatctg agcgtgacct ctctccctga
     7501 agcctccgtg gtgtgtccca atcaggacta caacctcgtc aatagcctgc tgaatctgac
     7561 cagaagccca gatggtagga tcgctgtcaa agcctgtgaa ggactgatgc tgctcgtgag
     7621 cctgcctgag cctgctgctg ccaagtgcct gactcaaagc acctgtctct gcgagctgct
     7681 gactgataga ctcgcatctc tctacaaggc actgccacag agcgttgatc ctctggacat
     7741 cgaaaccgtc gaggccatca actggggcct ggacagctac tctcataagg aggacgcttc
     7801 tgcctttcct ggaaagagag ccctgatttc tttcctgagc tggttcgatt actgcgacca
     7861 gctcatcaag gaagcacaga agacagcagc cgttgctctc gcaaaggctg tgcatgagag
     7921 attcttcatc ggtgtgatgg agcctcaact gatgcagacc tccgagatgg gcattctgac
     7981 cagcaccgca ctgctccatc gcatcgtgag acaagtgaca tccgacgtcc tgctccagga
     8041 gatggtgttc ttcatcctcg gagaacaacg cgagccagaa accctggcag aaatctccag
     8101 acatcctctg aggcacagac tcatcgaaca ctgtgaccac atctccgatg agatttccat
     8161 catgaccctg aggatgttcg agcatctgct ccagaagcca aatgagcata tcctgtacaa
     8221 cctcgtgctg agaaacctgg aggaaaggaa ctacacagag tacaagccac tgtgccctga
     8281 ggacaaggac gtcgtggaga atggcctgat tgctggagcc gtcgatctgg aggaagatcc
     8341 tctcttcaca gacatctctc ccgagaatac cctgccaaac caggagtggc tgtcctcttc
     8401 tcctcctgct acacccgacc accctaagaa cgatggcaag actgaggtgc acaagatcgt
     8461 caactccttc ctctgcctgg tccctgatga tgctaagtct tcctatcacg ttgagggtac
     8521 tggatacgac acatacctca gagacgcaca caggcagttc agggactatt gcgctatctg
     8581 cctcagatgg gagtggcctg gtagccctaa agccctggag aagtgtaacc tggaagcagc
     8641 attcttcgaa ggacactttc tcaaggtgct gttcgacaga atgggtagga ttctggacca
     8701 gccctatgat gtgaatctgc aagtcacatc cgtcctgtcc agactcagcc tgtttccaca
     8761 ccctcatatc catgagtatc tgctggaccc ttacgtgaac ctggcaccag gatgcaggtc
     8821 cctcttcagc gtgatcgtga gggtggtggg tgatctgatg ctcaggattc agagaatcca
     8881 agatttcact cctaaactgc tgctggtccg caagagactg ctgggtctgg agccagaggg
     8941 tccaatcatt gaccacatca ccctcctgga aggcgtgatt gtgctggagg agttctgcaa
     9001 ggaactggca gctatcgctt tcgtgaagta ccatgcctct tccactccct aaggtacctc
     9061 tagagcctgc agtctcgaca agcttgtcga gaagtactag aggatcataa tcagccatac
     9121 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     9181 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     9241 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9301 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac tgcttgagcc
     9361 tagaagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     9421 agcaataact atcataacca tttaaataaa tctatatctc aatcggggtt agtcgatacc
     9481 ccaccgagac cccattggga ccaatacgcc cgcgtttctt ccttttcccc accccaaccc
     9541 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcaag ccctgccata
     9601 gccactacgg gttaattaaa aactatatct caatcggggt ttaaacagga gatccgaacc
     9661 agataagtga aatctagttc caaactattt tgtcattttt aattttcgta ttagcttacg
     9721 acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct taaaaactcc
     9781 atttccaccc ctcccagttc ccaactattt tgtccgccca ca
//
