LOCUS       sequence-44359-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Munger K, 
            Harper JW, Howley PM
  TITLE     Systematic identification of interactions between host cell proteins
            and E7 oncoproteins from diverse human papillomaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 9.
  PUBMED    22232672
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Jan 9."
COMMENT     Sequence Label: p6643 MSCV-P C-FlagHA 31E7-Kozak
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     70..92
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     108..124
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     152..171
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        152..170
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    200..224
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(519..543)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             575..598
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             614..640
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             614..640
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(688..711)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cgttgccctc tgggtagcct tgtcacccta agcctccgcc tcctcttcct ccatccgccc
       61 cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc
      121 tcactccttc tctaggcgcc ggaattagat ctaatacgac tcactatagg gagacccaag
      181 ctggctagtt aagctatcaa caagtttgta caaaaaagtt ggcaccatgc gtggagaaac
      241 acctacgttg caagactatg tgttagattt gcaacctgag gcaactgacc tccactgtta
      301 tgagcaatta cccgacagct cagatgagga ggatgtcata gacagtccag ctggacaagc
      361 agaaccggac acatccaatt acaatatcgt taccttttgt tgtcagtgta agtctacact
      421 tcgtttgtgt gtacagagca cacaagtaga tattcgcata ttgcaagagc tgttaatggg
      481 ctcatttgga atcgtgtgcc ccaactgttc tactagactg ccaactttct tgtacaaagt
      541 ggttgatcta gagggcccgc ggttcgaact cgaggactac aaggacgacg atgacaagtc
      601 ggccgctgga ggatacccct acgacgtgcc cgactacgcc tagaattcta ccgggtaggg
      661 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
      721 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
      781 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
      841 ccccccgccc cgcagctcgc gtcgtgcagg acgtga
//
