LOCUS       sequence-443593-       12843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of SB100X Transposon with 10 kb cargo gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12843)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 12843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pMV-SB100X-10 kb
FEATURES             Location/Qualifiers
     source          1..12843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    797..878
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     822..841
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    5353..5747
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(6188..6204)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6188..6204)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6216..6233)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6387..6975)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6467..6486)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7146..7937,7938..8006))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     7769..7788
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8007..8111)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8179..8197
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     8206..8223
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8207..8223
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8673..9172
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8708..8731)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9105..9124
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      9186..9195
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9192..9791
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     9691..9711
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             9798..9854
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(9855..9857,9858..9860,9861..10574)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9855..9857,9858..9860,9861..10571)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9900..9921)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10161..10180)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10508..10529
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(10596..10613)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10602..10826
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        10872..12050
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          11103..12041
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     11998..12018
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      12065..12074
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12074..12097
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 caggtccgag gacgaggccg attattactg tatgatttgg catagttcag ccgctgtttt
       61 cggtggaggc acgcaactga cagtgctgac cacgacgcca gcgccgcgac caccaacacc
      121 ggcgcccacc atcgcgtcgc agcccctgtc cctgcgccca gaggcgtgcc ggccagcggc
      181 ggggggcgca gtgcacacga gggggctgga cttcgcctgt gatttttggg tgctggtggt
      241 ggttggggga gtcctggctt gctatagctt gctagtaaca gtggccttta ttattttctg
      301 ggtgaggagt aagaggagca ggctcctgca cagtgactac atgaacatga ctccccgccg
      361 ccccgggccc acccgcaagc attaccagcc ctatgcccca ccacgcgact tcgcagccta
      421 tcgctccaga gtgaagttca gcaggagcgc agacgccccc gcgtaccagc agggccagaa
      481 ccagctctat aacgagctca atctaggacg aagagaggag tacgatgttt tggacaagag
      541 acgtggccgg gaccctgaga tggggggaaa gccgagaagg aagaaccctc aggaaggcct
      601 gtacaatgaa ctgcagaaag ataagatggc ggaggcctac agtgagattg ggatgaaagg
      661 cgagcgccgg aggggcaagg ggcacgatgg cctttaccag ggtctcagta cagccaccaa
      721 ggacacctac gacgcccttc acatgcaggc cctgccccct cgcatcgatt gagtttggac
      781 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      841 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      901 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      961 aatgtggtac ttaagagggg gagaccaaag ggaagcttcc ttttgccacc tagctgtcca
     1021 ggggtgcctt aaaatggcaa acaaggtttg ttttcttttc ctgttttcat gccttcctct
     1081 tccatatcct tgtttcatat taatacatgt gtatagatcc taaaaatcta tacacatgta
     1141 ttaataaagc ctgattctgc cgcttctagg tatagaggcc acctgcaaga taaatatttg
     1201 attcacaata actaatcatt ctatggcaat tgataacaac aaatatatat atatatatat
     1261 atatacgtat atgtatatat atatatatat atattcagga aataatatat tctagaatat
     1321 gtcacattct gtctcaggca tccattttct ttatgatgcc gtttgaggtg gagttttagt
     1381 caggtggtca gcttctcctt ttttttgcca tctgccctgt aagcatcctg ctggggaccc
     1441 agataggagt catcactcta ggctgagaac atctgggcac acaccctaag cctcagcatg
     1501 actcatcatg actcagcatt gctgtgcttg agccagaagg tttgcttaga aggttacaca
     1561 gaaccagaag gcgggggtgg ggcactgacc ccgacagggg cctggccaga actgctcatg
     1621 cttggactat gggaggtcac taatggagac acacagaaat gtaacaggaa ctaaggaaaa
     1681 actgaagctt atttaatcag agatgaggat gctggaaggg atagagggag ctgagcttgt
     1741 aaaaagtata gtaatcattc agcaaatggt tttgaagcac ctgctggatg ctaaacacta
     1801 ttttcagtgc ttgaatcata aataagaata aaacatgtat cttattcccc acaagagtcc
     1861 aagtaaaaaa taacagttaa ttataatgtg ctctgtcccc caggctggag tgcagtggca
     1921 cgatctcagc tcactgcaac ctccgcctcc cgggttcaag caattctcct gcctcagcca
     1981 ccctaatagc tgggattaca ggtgcacacc accatgccag gctaattttt gtactttttg
     2041 tagaggcagg gtatcaccat gttgtccaag atggtcttga actcctgagc tccaagcagt
     2101 ccacccacct cagcctccca aagtgctggg attacaggtg tgagacacca tgcccagatt
     2161 ttccatattt aatagaggta tttatgggat gggggaaaag aatgtttctc tcactgtgga
     2221 ttattttaga gagtggagaa tggtcaagat ttttttaaaa attaagaaaa cataagttgg
     2281 accttgagaa atgaaaattt atttttttgt tggaggatac ccattctcta tctcccatca
     2341 gggcaagctg taaggaactg gctaagacac ggtgagacag agtgacttag tcttagaggc
     2401 cccactggta caagctttca ttaaaaaaag tctaaccagc tgcattcgac tttgactgca
     2461 gcagctggtt agaaggttct actggaggag ggtcccagcc cattgctaaa ttaacatcag
     2521 gctctgagac tggcagtata tctctaacag tggttgatgc tatcttctgg aacttgcctg
     2581 ctacattgag accactgacc catacatagg aagcccatag ctctgtcctg aactgttagg
     2641 ccactggtcc agagagtgtg catctccttt gatcctcata ataaccctat gagatagaca
     2701 caattattac tcttacttta tagatgatga tcctgaaaac ataggagtca aggcacttgc
     2761 ccctagctgg gggtataggg gagcagtccc atgtagtagt agaatgaaaa atgctgctat
     2821 gctgtgcctc ccccaccttt cccatgtctg ccctctactc atggtctatc tctcctggct
     2881 cctgggagtc atggactcca cccagcacca ccaacctgac ctaaccacct atctgagcct
     2941 gccagcctat aacccatctg ggccctgata gctggtggcc agccctgacc ccaccccacc
     3001 ctccctggaa cctctgatag acacatctgg cacaccagct cgcaaagtca ccgtgagggt
     3061 cttgtgtttg ctgagtcaaa attccttgaa atccaagtcc ttagagactc ctgctcccaa
     3121 atttacagtc atagacttct tcatggctgt ctcctttatc cacagaatga ttcctttgct
     3181 tcattgcccc atccatctga tcctcctcat cagtgcagca cagggcccat gagcagtagc
     3241 tgcagagtct cacataggtc tggcactgcc tctgacatgt ccgaccttag gcaaatgctt
     3301 gactcttctg agctcagtct tgtcatggca aaataaagat aataatagtg tttttttatg
     3361 gagttagcgt gaggatggaa aacaatagca aaattgatta gactataaaa ggtctcaaca
     3421 aatagtagta gattttatca tccattaatc cttccctctc ctctcttact catcccatca
     3481 cgtatgcctc ttaattttcc cttacctata ataagagtta ttcctcttat tatattcttc
     3541 ttatagtgat tctggatatt aaagtgggaa tgaggggcag gccactaacg aagaagatgt
     3601 ttctcaaaga agcgtaaata cacttgcaaa ggaggatgtt tttagtagca atttgtactg
     3661 atggtatggg gccaagagat atatcttaga gggagggctg agggtttgaa gtccaactcc
     3721 taagccagtg ccagaagagc caaggacagg tacggctgtc atcacttaga cctcaccctg
     3781 tggagccaca ccctagggtt ggccaatcta ctcccaggag cagggagggc aggagccagg
     3841 gctgggcata aaagtcaggg cagagccatc tattgcttac atttgcttct gacacaactg
     3901 tgttcactag caacctcaaa cagacaccat ggtgcacctg actcctgagg agaagtctgc
     3961 cgttactgcc ctgtggggca aggtgaacgt ggatgaagtt ggtggtgagg ccctgggcag
     4021 gttggtatca aggttacaag acaggtttaa ggagaccaat agaaactggg catgtggaga
     4081 cagagaagac tcttgggttt ctgataggca ctgactctct ctgcctattg gtctattttc
     4141 ccacccttag gctgctggtg gtctaccctt ggacccagag gttctttgag tcctttgggg
     4201 atctgtccac tcctgatgct gttatgggca accctaaggt gaaggctcat ggcaagaaag
     4261 tgctcggtgc ctttagtgat ggcctggctc acctggacaa cctcaagggc acctttgcca
     4321 cactgagtga gctgcactgt gacaagctgc acgtggatcc tgagaacttc agggtgagtc
     4381 tatgggaccc ttgatgtttt ctttcccctt cttttctatg gttaagttca tgtcatagga
     4441 aggggagaag taacagggta cagtttagaa tgggaaacag acgaatgatt gcatcagtgt
     4501 ggaagtctca ggatcgtttt agtttctttt atttgctgtt cataacaatt gttttctttt
     4561 gtttaattct tgctttcttt ttttttcttc tccgcaattt ttactattat acttaatgcc
     4621 ttaacattgt gtataacaaa aggaaatatc tctgagatac attaagtaac ttaaaaaaaa
     4681 actttacaca gtctgcctag tacattacta tttggaatat atgtgtgctt atttgcatat
     4741 tcataatctc cctactttat tttcttttat ttttaattga tacataatca ttatacatat
     4801 ttatgggtta aagtgtaatg ttttaatatg tgtacacata ttgaccaaat cagggtaatt
     4861 ttgcatttgt aattttaaaa aatgctttct tcttttaata tacttttttg tttatcttat
     4921 ttctaatact ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct
     4981 ctttgcacca ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt
     5041 ctgcatataa atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa
     5101 tagcagctac aatccagcta ccattctgct tttattttat ggttgggata aggctggatt
     5161 attctgagtc caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca
     5221 cagctcctgg gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa agaattcacc
     5281 ccaccagtgc aggctgccta tcagaaagtg gtggctggtg tggctaatgc cctggcccac
     5341 aagtatcact aagctcgctt tcttgctgtc caatttctat taaaggttcc tttgttccct
     5401 aagtccaact actaaactgg gggatattat gaagggcctt gagcatctgg attctgccta
     5461 ataaaaaaca tttattttca ttgcaatgat gtatttaaat tatttctgaa tattttacta
     5521 aaaagggaat gtgggaggtc agtgcattta aaacataaag aaatgaagag ctagttcaaa
     5581 ccttgggaaa atacactata tcttaaactc catgaaagaa ggtgaggctg caaacagcta
     5641 atgcacattg gcaacagccc ctgatgccta tgccttattc atccctcaga aaaggattca
     5701 agtagaggct tgatttggag gttaaagttt tgctatgctg tattttacat tacttattgt
     5761 tttagctgtc ctcatgggcc ctcgagcctt gaaatacatc cacaggtaca cctccaattg
     5821 actcaaatga tgtcaattag tctatcagaa gcttgtggaa ggctactcga aatgtttgac
     5881 ccaagttaaa caatttaaag gcaatgctac caaatactaa ttgagtgtat gtaaacttct
     5941 gacccactgg gaatgtgatg aaagaaataa aagctgaaat gaatcattct ctctactatt
     6001 attctgatat ttcacattct taaaataaag tggtgatcct aactgaccta agacagggaa
     6061 tttttactag gattaaatgt caggaattgt gaaaaagtga gtttaaatgt atttggctaa
     6121 ggtgtatgta aacttccgac ttcaactgta taggggccca gagacggagt cactgccaac
     6181 cgagacggtc atagctgttt cctgtgtgcc gcttcctcgc tcactgactc gctgcgctcg
     6241 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttacccaca
     6301 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6361 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6421 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6481 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6541 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6601 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6661 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6721 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6781 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6841 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6901 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6961 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7021 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7081 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7141 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct ctttcgttca
     7201 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7261 ggccccagtg ctgcaataat accgcgggac ccacgctcac cggctccaga tttatcagca
     7321 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7381 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7441 cgcaacgttg ttgccatcgc tacaggcatc gtggtatcac gctcgtcgtt tggtatggct
     7501 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgcgcaaa
     7561 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgccgtgtta
     7621 tcactcatgg ttatggcagc actacataat tctcttactg tcatgccatc cgtaagatgc
     7681 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7741 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7801 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7861 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7921 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7981 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8041 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8101 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     8161 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgttgtaa aacgacggcc
     8221 agtcgaacca cgcaatgcgt ctcgatccgc agtgtcttgc gtctctacta gtctatacag
     8281 ttgaagtcgg aagtttacat acacttaagt tggagtcatt aaaactcgtt tttcaactac
     8341 tccacaaatt tcttgttaac aaacaatagt tttggcaagt cagttaggac atctactttg
     8401 tgcatgacac aagtcatttt tccaacaatt gtttacagac agattatttc acttataatt
     8461 cactgtatca caattccagt gggtcagaag tttacataca ctaagttgac tgtgccttta
     8521 aacagcttgg aaaattccag aaaatgatgt catggcttta gaagcttccc acaataagtt
     8581 gggtgaattt tggctcattc ctcctttcta taggattgag gtcagagctt tgtgatggaa
     8641 ttctgtggaa taccggtgct agcaattcta ccgggtaggg gaggcgcttt tcccaaggca
     8701 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     8761 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     8821 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     8881 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     8941 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     9001 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     9061 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     9121 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     9181 ccaacgccac catggggacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     9241 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     9301 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     9361 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     9421 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     9481 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     9541 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     9601 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     9661 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     9721 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     9781 agcccggtgc catcgatgcc actaacttct ccctgttgaa acaagcaggg gatgtcgaag
     9841 agaatcccgg gccaatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     9901 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     9961 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
    10021 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
    10081 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
    10141 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
    10201 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
    10261 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
    10321 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
    10381 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
    10441 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
    10501 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
    10561 agctgtacaa gtaattaatt aagctcgctg atcagcctcg actgtgcctt ctagttgcca
    10621 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
    10681 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
    10741 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
    10801 tgctggggat gcggtgggct ctatggcttc tgagggcggc cgccaatggg taccttggtt
    10861 aattaacgtg aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
    10921 agaagttggg gggaggggtc ggcaattgac ccggtgccta gagaaggtgg cgcggggtaa
    10981 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
    11041 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
    11101 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
    11161 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg
    11221 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
    11281 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
    11341 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
    11401 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
    11461 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
    11521 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct cgccggcctg
    11581 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
    11641 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
    11701 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
    11761 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac tgagtaccgg gcgccgtcca
    11821 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
    11881 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
    11941 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
    12001 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga gggatccacc
    12061 ggtcgccacc atggattaca aagacgatga cgataaggcc ttaccagtga ccgccttgct
    12121 cctgccgctg gccttgctgc tccacgccgc caggccgcaa gtgcaactcc aacaatctgg
    12181 ccccggtctg gtcactcctt cccagactct tagtcttacc tgtgccatat caggtgattc
    12241 agtgagtagt aactccgcga catggaattg gatcaggcag tcccccagtc gagggttgga
    12301 atggctcggt agaacatact accgcagtaa atggtataat gattatgctg taagtgtgaa
    12361 aagcaggatg tctataaatc cagatacttc caaaaaccag ttctctcttc agcttaatag
    12421 tgtaactccc gaagacacag ctgtttacta ttgtgcgcgg ggtatgatga cttactacta
    12481 tggtatggac gtttggggac aaggtacaac cgtcaccgtg tccagtggga tcctgggctc
    12541 aggcggtgga ggttccggtg ggggcgggtc cggagggggg ggtagccaac ctgttctcac
    12601 gcaatcatcc tccctcagtg caagccccgg tgcctccgct tctttgacgt gtacactgag
    12661 gtcaggaatc aatgtagggc cttacaggat ctactggtat cagcaaaaac caggttctcc
    12721 tccccaatac cttctcaatt acaagagcga ctcagataag cagcagggat ctggagttcc
    12781 atcaaggttt agtggatcta aagacgcaag cgccaatgcg ggtgtgctgc tcatctcagg
    12841 tct
//
