LOCUS       sequence-44360-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Munger K, 
            Harper JW, Howley PM
  TITLE     Systematic identification of interactions between host cell proteins
            and E7 oncoproteins from diverse human papillomaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 9.
  PUBMED    22232672
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Jan 9."
COMMENT     Sequence Label: p6656 MSCV-P C-FlagHA 25E7-Kozak
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     73..95
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     111..127
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     155..174
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        155..173
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    203..227
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             238..246
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(534..558)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             590..613
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             629..655
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             629..655
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(703..726)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cgttgccctc tgggtcagcc ctttgtcacc ctaagcctcc gcctcctctt cctccatccg
       61 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
      121 ccctcactcc ttctctaggc gccggaatta gatctaatac gactcactat agggagaccc
      181 aagctggcta gttaagctat caacaagttt gtacaaaaaa gttggcacca tgattggtaa
      241 ggaggtcaca ttgcaagatt ttacattaga gttaagtgaa ttgcagcctg aggtacaacc
      301 agttgacctg ttttgtgaag aggagttgcc ggctgagcat caggaaacag aggaggagcc
      361 tgctattgac agaactccat acaaagttgt tgcgccttgt ggttgctgcg aggtcaagct
      421 tcgcatcttt gtgaaagcca ctgattttgg tattagaaca ctacaaaacc ttctaattga
      481 agaactgcag ctgttgtgtc cggagtgtcg cgggaactgc aaacatggcg gatccccaac
      541 tttcttgtac aaagtggttg atctagaggg cccgcggttc gaactcgagg actacaagga
      601 cgacgatgac aagtcggccg ctggaggata cccctacgac gtgcccgact acgcctagaa
      661 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
      721 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
      781 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
      841 ctagtcagga agttcccccc gccccgcagc tcgcgtcgt
//
