LOCUS       sequence-443603-        3217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of MAG transposase 
            mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pICOZ-MAG-H181V
FEATURES             Location/Qualifiers
     source          1..3217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      279..288
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(353..372)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(354..372)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(417..620)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(446..466)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        621..1000
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(1023..1040)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1023..1039)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1077..1139
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1140..1514
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      1539..2127
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2028..2047
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2173..2189
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2173..2189
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2192..2416
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2405..2422
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        2442..2460
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2442..2459
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 aacggtgcga tcgctaacgc tcagctgcag ttcatcacga atggcgcgcg cgcttttaaa
       61 cgggtcggct ttacacagac gggtcatacg tgcatcatca cgcgcgctag ttttacgagg
      121 gcggccagta ggaattttgg tgccggtgct atgcagggcg ttatacacaa aggtcttgct
      181 gcggcccagg atttccgcaa ttttgcgttg gctataaccg gcggcgctta agcgtttaat
      241 gtccaggcgc tgttgttggg tgctatgcgc tgaacgaccc atggtggcgg cgaattccag
      301 cacactggcg gccgttacta gtggatccga gctcggtacc aagcttgggt ctccctatag
      361 tgagtcgtat taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct
      421 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
      481 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
      541 tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg
      601 tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa
      661 gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca
      721 ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca
      781 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg
      841 ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg
      901 ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc
      961 gtaattgatt actattaata actagtcaat aatcaatgtc aacgcgtata tctggcccgt
     1021 cgactggccg tcgttttaca aggccatggg ttaacccata tggcctcgcg acgcgttatg
     1081 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aggaagagta
     1141 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     1201 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     1261 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     1321 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     1381 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     1441 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     1501 aggagcagga ctgacgtaga aaagatcaaa ggatcttctt gagatccttt tttcctgcgc
     1561 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttt tttgccggat
     1621 caagagctac caactctttt tccgaaggta actggcttca gcagagcgcg gataccaaat
     1681 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1741 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1801 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1861 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1921 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1981 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2041 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2101 tcgtcagggg ggcggagcct atggaaaaac acatgtgttt aaacgggccc actgtctcga
     2161 ggatatccca cacaggaaac agctatgacc gccatagagc ccaccgcatc cccagcatgc
     2221 ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata
     2281 gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg
     2341 ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg
     2401 caactagaag gcacagtcga ggctgatcag cgagctctag catttaggtg acactataga
     2461 atagggccct ctagatgcat gctcgagcgg ccgctcatca atatttggtc gcatggccgt
     2521 tattgcgaat caccgcgcgc atacgtttcg gcatgctttc caccaggctt ttacaggtgc
     2581 tcggaggtat gctgtaccac gcgttttcaa tatgcttcca cagtttctct ttggtgcgtg
     2641 aacctgcagg aggcagcttg gccttcacaa tcttccacag gttttcgatc gggttcagat
     2701 cagggctctg cgcaggccag ttcatcacat caatcttctg gtccacgaac catttcttca
     2761 ccgctttggc ggtatgcttc gggtcgttat catgcatgaa ctgccacttc agaggcattt
     2821 cccattcggc gtgaggcagc atcacggtct gaataatgtt caggtagcga tgctgatcca
     2881 tgatgtcttt gatccagaag atagggccca cgccatacca tgagaagcac gcccacacca
     2941 taatgttgcc gccgccaact ttcacggttt tcagggtata ctgagggcga tacgcggtat
     3001 tcttagggcg gcgcacaaaa cgtttgccat cgctgcccac caggttcact ttgctctcat
     3061 cgctccacag cacgttgcgc catttgttta agttctcgcc cgcccaatca tagtgctcgc
     3121 gcgcaaattg taagcgtgcc tgcacatgac aacgacgcag cagaggcact ttacgaggat
     3181 tgcggcccac cagattattc tcgaacaggc gacgttg
//
