LOCUS       sequence-443604-        7926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of Tc1-8B_DR Transposon with 5 kb cargo gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 7926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pQLL-Tc1-8B_DR-5 kb
FEATURES             Location/Qualifiers
     source          1..7926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1179..1403
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1392..1409
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(1431..2144,2145..2147,2148..2150))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1434..2144,2145..2147,2148..2150))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1476..1497)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1825..1844
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2084..2105
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2151..2207)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2214..2813)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2294..2314)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     regulatory      2810..2819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2833..3332)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2881..2900)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3274..3297
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(3769..3786)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3769..3785)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3795..3813)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      3848..3877
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      3899..4486
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4387..4406
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4498..4525)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(4549..4740)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(4741..5532,5533..5601))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5364..5383
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5602..5704)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(5705..5736)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     5788..5805
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     5817..5833
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5817..5833
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6210..6291
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6247..6266)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7834..7857)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      7857..7866
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(join(7881..7926,1..1133))
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(7890..7926,1..902)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(join(7913..7926,1..7))
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggcttgagaa tgaaccaaga tccaaactca aaaagggcaa attccaagga gaattacatc
       61 aagtgccaag ctggcctaac ttcagtctcc acccactcag tgtggggaaa ctccatcgca
      121 taaaacccct ccccccaacc taaagacgac gtactccaaa agctcgagaa ctaatcgagg
      181 tgcctggacg gcgcccggta ctcagtggag tcacatgaag cgacggctga ggacggaaag
      241 gcccttttcc tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg cgtcctccat
      301 tttgagctcc ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc aactggtgcc
      361 gaccgggcca gccttgccgc ccagggcggg gcgatacacg gcggcgcgag gccaggcacc
      421 agagcaggcc ggcgagcttg agactacccc cgtccgattc tcggtggccg cgctcgcagg
      481 ccccgcctcg ccgaacatgt gcgctgggac gcacgggccc cgtcgccgcc cgcggcccca
      541 aaaaccgaaa taccagtgtg cagatcttgg cccgcattta caagactatc ttgccagaaa
      601 aaaagcgtcg cagcaggtca tcaaaaattt taaatggcta gagacttatc gaaagcagcg
      661 agacaggcgc gaaggtgcca ccagattcgc acgcggcggc cccagcgccc aggccaggcc
      721 tcaactcaag cacgaggcga aggggctcct taagcgcaag gcctcgaact ctcccaccca
      781 cttccaaccc gaagctcggg atcaagaatc acgtactgca gccaggtgga agtaattcaa
      841 ggcacgcaag ggccataacc cgtaaagagg ccaggcccgc gggaaccaca cacggcactt
      901 acctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac tactgcactt
      961 atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac atcactttcc
     1021 cagtttaccc cgcgccacct tctctaggca ccgggtcaat tgccgacccc tccccccaac
     1081 ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga gcctcacgtt
     1141 aattaaccaa ggtacccatt ggcggccgcc ctcagaagcc atagagccca ccgcatcccc
     1201 agcatgcctg ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc
     1261 cagaatagaa tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag
     1321 gacagtggga gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga
     1381 tggctggcaa ctagaaggca cagtcgaggc tgatcagcga gcttaattaa ttacttgtac
     1441 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
     1501 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
     1561 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
     1621 acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc
     1681 ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg
     1741 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
     1801 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
     1861 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
     1921 tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag
     1981 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
     2041 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
     2101 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat tggcccggga
     2161 ttctcttcga catcccctgc ttgtttcaac agggagaagt tagtggcatc gatggcaccg
     2221 ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg
     2281 gtgaagccga gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg
     2341 ggcaccccgg cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc
     2401 ttgccctggt ggtcgggcga gacgccgacg gtggccagga accacgcggg ctccttgggc
     2461 cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac
     2521 tcggccatgc gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg
     2581 gtccagaccg ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg
     2641 acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccgggtcg
     2701 acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggcccgg
     2761 gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt actcggtccc catggtggcg
     2821 ttggctgcag gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg cagacgtgcg
     2881 cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct
     2941 gagcccgccc ctgagcccgc ccccggaccc accccttccc agcctctgag cccagaaagc
     3001 gaaggagcaa agctgctatt ggccgctgcc ccaaaggcct acccgcttcc attgctcagc
     3061 ggtgctgtcc atctgcacga gactagtgag acgtgctact tccatttgtc acgtcctgca
     3121 cgacgcgagc tgcggggcgg gggggaactt cctgactagg ggaggagtag aaggtggcgc
     3181 gaaggggcca ccaaagaacg gagccggttg gcgcctaccg gtggatgtgg aatgtgtgcg
     3241 aggccagagg ccacttgtgt agcgccaagt gcccagcggg gctgctaaag cgcatgctcc
     3301 agactgcctt gggaaaagcg cctcccctac ccggtagaat tgctagcacc ggtgaagtct
     3361 ttcctgtgtg cctcctgagt aatgagaagc ctttataggc catcaattag gactaaagca
     3421 gctgatatca attagtactg atagggagca gggtttttct ctcaatactg acagatctcc
     3481 tgactttcta tgccattttg caccttgtta tcttcatgtg ttcaatactt attccctgtg
     3541 tcatttcact ttatttatta tgactcaact tgtatactta aatgttctga tttctttgta
     3601 tgaattcaat atttggcttg atggctacat ctggtaggaa ttttgtgtca gtagcccact
     3661 tagaaatccc cttactgata aaaatgctga tgtgtcaaat acttattttc cccgctgtac
     3721 atatgagaga cgcaagacac tgcggatcga gacgcattgc gtggttcgac tggccgtcgt
     3781 tttacaacga aagggcctcg tgatacgcct attgactcca gcgtaactgg actgcaatca
     3841 actcactggc tcaccttcac gggtgggcct ttcttcggta gaaaatcaaa ggatcttctt
     3901 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3961 cggtggtttg tttgccggat caagagctac caactctttt tccgaggtaa ctggcttcag
     4021 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4081 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4141 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4201 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4261 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4321 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4381 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4441 gcatcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4501 agaaaggccc acccgaaggt gagccaggtg attacatttg ggccctcatc agaggttttc
     4561 accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag
     4621 cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt
     4681 taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac
     4741 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4801 agttgcctga ctccccgtcg tgtagataac tacgatgcgg gagggcttac catctggccc
     4861 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
     4921 ccagccagcc gggagggccg agcgcagaag tgatcctgca actttatccg cctccatcca
     4981 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5041 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5101 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5161 ggttagctcc ttcggtcctc cgatcgttgc cagaagtaag ttggccgcag tgttatcact
     5221 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtga gatgcttttc
     5281 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5341 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5401 catcattgga aaacgttctt cggggcgtaa actctcaagg atcttaccgc tgttgagatc
     5461 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5521 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5581 acggaaatgt tgaatactca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     5641 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg
     5701 atcaagtcaa aagcctccgg tcggaggctt ttgactttct gctatggagg tcaggtatga
     5761 tttaaatggt cagtaacgac cgagcgcagc gagtcagtga gcgaggaagc ggcacacagg
     5821 aaacagctat gaccgtctcg gttggcagtg actccgtctc tgggccctac agcggggaaa
     5881 ataagtattt gacacatcag catttttatc agtaagggga tttctaagtg ggctactgac
     5941 acaaaattcc taccagatgt agccatcaag ccaaatattg aattcataca aagaaatcag
     6001 aacatttaag tatacaagtt gagtcataat aaataaagtg aaatgacaca gggaataagt
     6061 attgaacaca ctttacctcg agggccagga agcttccctt tggtctcccc ctcttaagta
     6121 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     6181 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     6241 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6301 gtggtttgtc caaactcaat cgatgcgagg gggcagggcc tgcatgtgaa gggcgtcgta
     6361 ggtgtccttg gtggctgtac tgagaccctg gtaaaggcca tcgtgcccct tgcccctccg
     6421 gcgctcgcct ttcatcccaa tctcactgta ggcctccgcc atcttatctt tctgcagttc
     6481 attgtacagg ccttcctgag ggttcttcct tctcggcttt ccccccatct cagggtcccg
     6541 gccacgtctc ttgtccaaaa catcgtactc ctctcttcgt cctagattga gctcgttata
     6601 gagctggttc tggccctgct ggtacgcggg ggcgtctgcg ctcctgctga acttcactct
     6661 ggagcgatag gctgcgaagt cgcgtggtgg ggcatagggc tggtaatgct tgcgggtggg
     6721 cccggggcgg cggggagtca tgttcatgta gtcactgtgc aggagcctgc tcctcttact
     6781 cctcacccag aaaataataa aggccactgt tactagcaag ctatagcaag ccaggactcc
     6841 cccaaccacc accagcaccc aaaaatcaca ggcgaagtcc agccccctcg tgtgcactgc
     6901 gccccccgcc gctggccggc acgcctctgg gcgcagggac aggggctgcg acgcgatggt
     6961 gggcgccggt gttggtggtc gcggcgctgg cgtcgtggtc agcactgtca gttgcgtgcc
     7021 tccaccgaaa acagcggctg aactatgcca aatcatacag taataatcgg cctcgtcctc
     7081 ggacctgaga cctgagatga gcagcacacc cgcattggcg cttgcgtctt tagatccact
     7141 aaaccttgat ggaactccag atccctgctg cttatctgag tcgctcttgt aattgagaag
     7201 gtattgggga ggagaacctg gtttttgctg ataccagtag atcctgtaag gccctacatt
     7261 gattcctgac ctcagtgtac acgtcaaaga agcggaggca ccggggcttg cactgaggga
     7321 ggatgattgc gtgagaacag gttggctacc cccccctccg gacccgcccc caccggaacc
     7381 tccaccgcct gagcccagga tcccactgga cacggtgacg gttgtacctt gtccccaaac
     7441 gtccatacca tagtagtaag tcatcatacc ccgcgcacaa tagtaaacag ctgtgtcttc
     7501 gggagttaca ctattaagct gaagagagaa ctggtttttg gaagtatctg gatttataga
     7561 catcctgctt ttcacactta cagcataatc attataccat ttactgcggt agtatgttct
     7621 accgagccat tccaaccctc gactggggga ctgcctgatc caattccatg tcgcggagtt
     7681 actactcact gaatcacctg atatggcaca ggtaagacta agagtctggg aaggagtgac
     7741 cagaccgggg ccagattgtt ggagttgcac ttgcggcctg gcggcgtgga gcagcaaggc
     7801 cagcggcagg agcaaggcgg tcactggtaa ggccttatcg tcatcgtctt tgtaatccat
     7861 ggtggcgacc ggtggatccc tcacgacacc tgaaatggaa gaaaaaaact ttgaaccact
     7921 gtctga
//
