LOCUS       sequence-443608-       10151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of Tc1-8B_DR Transposon with 10 kb cargo gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 10151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pQLL-Tc1-8B_DR-10 kb
FEATURES             Location/Qualifiers
     source          1..10151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        185..684
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(220..243)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     617..636
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      698..707
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             704..1303
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     1203..1223
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1310..1366
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1367..1369,1370..1372,1373..2086)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1367..1369,1370..1372,1373..2083)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1412..1433)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1673..1692)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2020..2041
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2108..2125)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2114..2338
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        2384..3562
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2615..3553
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3510..3530
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3577..3586
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3586..3609
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    5152..5233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     5177..5196
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    9708..10102
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
ORIGIN
        1 tgaagataac aaggtgcaaa atggcataga aagtcaggag atctgtcagt attgagagaa
       61 aaaccctgct ccctatcagt actaattgat atcagctgct ttagtcctaa ttgatggcct
      121 ataaaggctt ctcattactc aggaggcaca caggaaagac ttcaccggtg ctagcaattc
      181 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
      241 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
      301 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
      361 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
      421 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
      481 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
      541 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
      601 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
      661 tcttcctcat ctccgggcct ttcgacctgc agccaacgcc accatgggga ccgagtacaa
      721 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
      781 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg
      841 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
      901 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
      961 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     1021 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     1081 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     1141 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     1201 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     1261 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccatcgatg ccactaactt
     1321 ctccctgttg aaacaagcag gggatgtcga agagaatccc gggccaatgg tgagcaaggg
     1381 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1441 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     1501 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     1561 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     1621 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1681 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1741 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1801 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     1861 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     1921 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     1981 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     2041 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaattaa ttaagctcgc
     2101 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     2161 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     2221 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     2281 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     2341 tctgagggcg gccgccaatg ggtaccttgg ttaattaacg tgaggctccg gtgcccgtca
     2401 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     2461 acccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2521 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2581 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     2641 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     2701 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     2761 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     2821 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     2881 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     2941 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     3001 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     3061 cggacggggg tagtctcaag ctcgccggcc tgctctggtg cctggcctcg cgccgccgtg
     3121 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     3181 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     3241 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     3301 atgtgactcc actgagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     3361 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     3421 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     3481 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     3541 tcttccattt caggtgtcgt gagggatcca ccggtcgcca ccatggatta caaagacgat
     3601 gacgataagg ccttaccagt gaccgccttg ctcctgccgc tggccttgct gctccacgcc
     3661 gccaggccgc aagtgcaact ccaacaatct ggccccggtc tggtcactcc ttcccagact
     3721 cttagtctta cctgtgccat atcaggtgat tcagtgagta gtaactccgc gacatggaat
     3781 tggatcaggc agtcccccag tcgagggttg gaatggctcg gtagaacata ctaccgcagt
     3841 aaatggtata atgattatgc tgtaagtgtg aaaagcagga tgtctataaa tccagatact
     3901 tccaaaaacc agttctctct tcagcttaat agtgtaactc ccgaagacac agctgtttac
     3961 tattgtgcgc ggggtatgat gacttactac tatggtatgg acgtttgggg acaaggtaca
     4021 accgtcaccg tgtccagtgg gatcctgggc tcaggcggtg gaggttccgg tgggggcggg
     4081 tccggagggg ggggtagcca acctgttctc acgcaatcat cctccctcag tgcaagcccc
     4141 ggtgcctccg cttctttgac gtgtacactg aggtcaggaa tcaatgtagg gccttacagg
     4201 atctactggt atcagcaaaa accaggttct cctccccaat accttctcaa ttacaagagc
     4261 gactcagata agcagcaggg atctggagtt ccatcaaggt ttagtggatc taaagacgca
     4321 agcgccaatg cgggtgtgct gctcatctca ggtctcaggt ccgaggacga ggccgattat
     4381 tactgtatga tttggcatag ttcagccgct gttttcggtg gaggcacgca actgacagtg
     4441 ctgaccacga cgccagcgcc gcgaccacca acaccggcgc ccaccatcgc gtcgcagccc
     4501 ctgtccctgc gcccagaggc gtgccggcca gcggcggggg gcgcagtgca cacgaggggg
     4561 ctggacttcg cctgtgattt ttgggtgctg gtggtggttg ggggagtcct ggcttgctat
     4621 agcttgctag taacagtggc ctttattatt ttctgggtga ggagtaagag gagcaggctc
     4681 ctgcacagtg actacatgaa catgactccc cgccgccccg ggcccacccg caagcattac
     4741 cagccctatg ccccaccacg cgacttcgca gcctatcgct ccagagtgaa gttcagcagg
     4801 agcgcagacg cccccgcgta ccagcagggc cagaaccagc tctataacga gctcaatcta
     4861 ggacgaagag aggagtacga tgttttggac aagagacgtg gccgggaccc tgagatgggg
     4921 ggaaagccga gaaggaagaa ccctcaggaa ggcctgtaca atgaactgca gaaagataag
     4981 atggcggagg cctacagtga gattgggatg aaaggcgagc gccggagggg caaggggcac
     5041 gatggccttt accagggtct cagtacagcc accaaggaca cctacgacgc ccttcacatg
     5101 caggccctgc cccctcgcat cgattgagtt tggacaaacc acaactagaa tgcagtgaaa
     5161 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     5221 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     5281 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtacttaag agggggagac
     5341 caaagggaag cttccttttg ccacctagct gtccaggggt gccttaaaat ggcaaacaag
     5401 gtttgttttc ttttcctgtt ttcatgcctt cctcttccat atccttgttt catattaata
     5461 catgtgtata gatcctaaaa atctatacac atgtattaat aaagcctgat tctgccgctt
     5521 ctaggtatag aggccacctg caagataaat atttgattca caataactaa tcattctatg
     5581 gcaattgata acaacaaata tatatatata tatatatata cgtatatgta tatatatata
     5641 tatatatatt caggaaataa tatattctag aatatgtcac attctgtctc aggcatccat
     5701 tttctttatg atgccgtttg aggtggagtt ttagtcaggt ggtcagcttc tccttttttt
     5761 tgccatctgc cctgtaagca tcctgctggg gacccagata ggagtcatca ctctaggctg
     5821 agaacatctg ggcacacacc ctaagcctca gcatgactca tcatgactca gcattgctgt
     5881 gcttgagcca gaaggtttgc ttagaaggtt acacagaacc agaaggcggg ggtggggcac
     5941 tgaccccgac aggggcctgg ccagaactgc tcatgcttgg actatgggag gtcactaatg
     6001 gagacacaca gaaatgtaac aggaactaag gaaaaactga agcttattta atcagagatg
     6061 aggatgctgg aagggataga gggagctgag cttgtaaaaa gtatagtaat cattcagcaa
     6121 atggttttga agcacctgct ggatgctaaa cactattttc agtgcttgaa tcataaataa
     6181 gaataaaaca tgtatcttat tccccacaag agtccaagta aaaaataaca gttaattata
     6241 atgtgctctg tcccccaggc tggagtgcag tggcacgatc tcagctcact gcaacctccg
     6301 cctcccgggt tcaagcaatt ctcctgcctc agccacccta atagctggga ttacaggtgc
     6361 acaccaccat gccaggctaa tttttgtact ttttgtagag gcagggtatc accatgttgt
     6421 ccaagatggt cttgaactcc tgagctccaa gcagtccacc cacctcagcc tcccaaagtg
     6481 ctgggattac aggtgtgaga caccatgccc agattttcca tatttaatag aggtatttat
     6541 gggatggggg aaaagaatgt ttctctcact gtggattatt ttagagagtg gagaatggtc
     6601 aagatttttt taaaaattaa gaaaacataa gttggacctt gagaaatgaa aatttatttt
     6661 tttgttggag gatacccatt ctctatctcc catcagggca agctgtaagg aactggctaa
     6721 gacacggtga gacagagtga cttagtctta gaggccccac tggtacaagc tttcattaaa
     6781 aaaagtctaa ccagctgcat tcgactttga ctgcagcagc tggttagaag gttctactgg
     6841 aggagggtcc cagcccattg ctaaattaac atcaggctct gagactggca gtatatctct
     6901 aacagtggtt gatgctatct tctggaactt gcctgctaca ttgagaccac tgacccatac
     6961 ataggaagcc catagctctg tcctgaactg ttaggccact ggtccagaga gtgtgcatct
     7021 cctttgatcc tcataataac cctatgagat agacacaatt attactctta ctttatagat
     7081 gatgatcctg aaaacatagg agtcaaggca cttgccccta gctgggggta taggggagca
     7141 gtcccatgta gtagtagaat gaaaaatgct gctatgctgt gcctccccca cctttcccat
     7201 gtctgccctc tactcatggt ctatctctcc tggctcctgg gagtcatgga ctccacccag
     7261 caccaccaac ctgacctaac cacctatctg agcctgccag cctataaccc atctgggccc
     7321 tgatagctgg tggccagccc tgaccccacc ccaccctccc tggaacctct gatagacaca
     7381 tctggcacac cagctcgcaa agtcaccgtg agggtcttgt gtttgctgag tcaaaattcc
     7441 ttgaaatcca agtccttaga gactcctgct cccaaattta cagtcataga cttcttcatg
     7501 gctgtctcct ttatccacag aatgattcct ttgcttcatt gccccatcca tctgatcctc
     7561 ctcatcagtg cagcacaggg cccatgagca gtagctgcag agtctcacat aggtctggca
     7621 ctgcctctga catgtccgac cttaggcaaa tgcttgactc ttctgagctc agtcttgtca
     7681 tggcaaaata aagataataa tagtgttttt ttatggagtt agcgtgagga tggaaaacaa
     7741 tagcaaaatt gattagacta taaaaggtct caacaaatag tagtagattt tatcatccat
     7801 taatccttcc ctctcctctc ttactcatcc catcacgtat gcctcttaat tttcccttac
     7861 ctataataag agttattcct cttattatat tcttcttata gtgattctgg atattaaagt
     7921 gggaatgagg ggcaggccac taacgaagaa gatgtttctc aaagaagcgt aaatacactt
     7981 gcaaaggagg atgtttttag tagcaatttg tactgatggt atggggccaa gagatatatc
     8041 ttagagggag ggctgagggt ttgaagtcca actcctaagc cagtgccaga agagccaagg
     8101 acaggtacgg ctgtcatcac ttagacctca ccctgtggag ccacacccta gggttggcca
     8161 atctactccc aggagcaggg agggcaggag ccagggctgg gcataaaagt cagggcagag
     8221 ccatctattg cttacatttg cttctgacac aactgtgttc actagcaacc tcaaacagac
     8281 accatggtgc acctgactcc tgaggagaag tctgccgtta ctgccctgtg gggcaaggtg
     8341 aacgtggatg aagttggtgg tgaggccctg ggcaggttgg tatcaaggtt acaagacagg
     8401 tttaaggaga ccaatagaaa ctgggcatgt ggagacagag aagactcttg ggtttctgat
     8461 aggcactgac tctctctgcc tattggtcta ttttcccacc cttaggctgc tggtggtcta
     8521 cccttggacc cagaggttct ttgagtcctt tggggatctg tccactcctg atgctgttat
     8581 gggcaaccct aaggtgaagg ctcatggcaa gaaagtgctc ggtgccttta gtgatggcct
     8641 ggctcacctg gacaacctca agggcacctt tgccacactg agtgagctgc actgtgacaa
     8701 gctgcacgtg gatcctgaga acttcagggt gagtctatgg gacccttgat gttttctttc
     8761 cccttctttt ctatggttaa gttcatgtca taggaagggg agaagtaaca gggtacagtt
     8821 tagaatggga aacagacgaa tgattgcatc agtgtggaag tctcaggatc gttttagttt
     8881 cttttatttg ctgttcataa caattgtttt cttttgttta attcttgctt tctttttttt
     8941 tcttctccgc aatttttact attatactta atgccttaac attgtgtata acaaaaggaa
     9001 atatctctga gatacattaa gtaacttaaa aaaaaacttt acacagtctg cctagtacat
     9061 tactatttgg aatatatgtg tgcttatttg catattcata atctccctac tttattttct
     9121 tttattttta attgatacat aatcattata catatttatg ggttaaagtg taatgtttta
     9181 atatgtgtac acatattgac caaatcaggg taattttgca tttgtaattt taaaaaatgc
     9241 tttcttcttt taatatactt ttttgtttat cttatttcta atactttccc taatctcttt
     9301 ctttcagggc aataatgata caatgtatca tgcctctttg caccattcta aagaataaca
     9361 gtgataattt ctgggttaag gcaatagcaa tatttctgca tataaatatt tctgcatata
     9421 aattgtaact gatgtaagag gtttcatatt gctaatagca gctacaatcc agctaccatt
     9481 ctgcttttat tttatggttg ggataaggct ggattattct gagtccaagc taggcccttt
     9541 tgctaatcat gttcatacct cttatcttcc tcccacagct cctgggcaac gtgctggtct
     9601 gtgtgctggc ccatcacttt ggcaaagaat tcaccccacc agtgcaggct gcctatcaga
     9661 aagtggtggc tggtgtggct aatgccctgg cccacaagta tcactaagct cgctttcttg
     9721 ctgtccaatt tctattaaag gttcctttgt tccctaagtc caactactaa actgggggat
     9781 attatgaagg gccttgagca tctggattct gcctaataaa aaacatttat tttcattgca
     9841 atgatgtatt taaattattt ctgaatattt tactaaaaag ggaatgtggg aggtcagtgc
     9901 atttaaaaca taaagaaatg aagagctagt tcaaaccttg ggaaaataca ctatatctta
     9961 aactccatga aagaaggtga ggctgcaaac agctaatgca cattggcaac agcccctgat
    10021 gcctatgcct tattcatccc tcagaaaagg attcaagtag aggcttgatt tggaggttaa
    10081 agttttgcta tgctgtattt tacattactt attgttttag ctgtcctcat gggcgcgccc
    10141 tcgaggtaaa g
//
