LOCUS       sequence-443622-        9252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral CROP-seq vector with lineage barcode cassette and T7 
            promoter for ID amplification from cDNA, engineered for 
            high-efficiency ID assignment in Multiome Perturb-seq experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9252)
  AUTHORS   Metzner E, Southard KM, Norman TM
  TITLE     Multiome Perturb-seq unlocks scalable discovery of integrated 
            perturbation effects on the transcriptome and epigenome.
  JOURNAL   Cell Syst. 2025 Jan 15;16(1):101161. doi: 
            10.1016/j.cels.2024.12.002. Epub 2024 Dec 16.
  PUBMED    39689711
REFERENCE   2  (bases 1 to 9252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-01-15"; volume: "16(1):101161. doi"; pages: " 
            10.1016/j.cels.2024.12.002. Epub 2024 Dec 16"
COMMENT     Sequence Label: pEM040 [Multiome Perturb-seq sgRNA expression
            ...
FEATURES             Location/Qualifiers
     source          1..9252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    10..31
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(148..165)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(319..907)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(399..418)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1078..1869,1870..1938))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1701..1720
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1939..2043)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2069..2524)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2206..2227)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2418..2437
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2666..2682
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2692..2710
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2731..2866
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2885..3006
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2896..2915)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2950..2969
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3084..3264
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     4608..4624
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4705..4725)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    4729..4762
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(4969..4987)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(5173..5886,5887..5889,5890..5892))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5176..5886,5887..5889,5890..5892))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5218..5239)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5826..5847
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5889..5898
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5953..7131)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5962..6900
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5985..6005)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7270..7387
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7568..7612)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7797..8030
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8523..8648
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8695..8875
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8876..9102)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(9130..9150)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9130..9148)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9169..9185)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9169..9185)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9182..9204)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9193..9209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9217..9223,9224..9241,9242..9247))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
       61 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
      121 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
      181 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      241 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      301 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      361 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      421 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      481 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      541 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      601 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      661 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      721 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
      781 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      841 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      901 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      961 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1021 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     1081 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     1141 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     1201 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     1261 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     1321 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     1381 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     1441 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     1501 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     1561 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     1621 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     1681 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     1741 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     1801 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     1861 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     1921 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     1981 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     2041 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     2101 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     2161 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     2221 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     2281 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     2341 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     2401 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     2461 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     2521 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     2581 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     2641 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
     2701 tcactatagg gcgaattggg tacgtcccta ggcctccaaa aaagcctcct cactacttct
     2761 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     2821 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatagct agagccagac
     2881 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     2941 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     3001 caagttcctc tcactctctg atattcattt ctttgcaagt tataaatact gaataataag
     3061 atgacatgaa ctactatacg tactgctaga gattttccac actgactaaa agggtctgag
     3121 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     3181 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     3241 gatctggtct aaccagagag acccagtaca agcaaaaagc agtgtctgtc agctatgcac
     3301 tggcgattgt ggatccttgt gtcagttgtc gggtcgacct ccgcggttga atcttgggaa
     3361 ctctcgggag gctgaggcag gagaatcgct tgaacccggg aggcgctcgt gagtctggac
     3421 ctttatcgca aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc
     3481 ttatttaaac ttgctatgct gtttccagct tagctcttaa accaccatcg tctgctcatc
     3541 catgacctga ccatgcagag gatgatgctc gtgacggtta acgcctcgaa tcagcaacgg
     3601 cttgccgttc agcagcagca gaccattttc aatccgcacc tcgcggaaac cgacatcgca
     3661 ggcttctgct tcaatcagcg tgccgtcggc ggtgtgcagt tcaaccaccg cacgatagag
     3721 attcgggatt tcggcgctcc acagtttcgg gttttcgacg ttcagacgta gtgtgacgcg
     3781 atcggcataa ccaccacgct catcgataat ttcaccgccg aaaggcgcgg tgccgctggc
     3841 gacgtgcgtt tcaccctgcc ataaagaaac tgttacccgt aggtagtcac gcaactcgcc
     3901 gcacatctga acttcagcct ccagtacagc gcggctgaaa tcatcattaa agcgagtggc
     3961 aacatggaaa tcgctgattt gtgtagtcgg tttatgcagc aacgtgatgt cacggaaaat
     4021 gccgctcatc cgccacatat cctgatcttc cagataactg ccgtcactcc agcgcagcac
     4081 catcaccgcg aggcggtttt ctccggcgcg taaaaatgcg ctcaggtcaa attcagacgg
     4141 caaacgactg tcctggccgt aaccgaccca gcgcccgttg caccacagat gaaacgccga
     4201 tttaatgcca tcaaaaataa ttcgcgtctg gccttcctgt agccagcttt catcaacatt
     4261 aaatgtgagc gagtaacaac ccgtcggatt ctccgtggga acaaacggcg gattgaccgt
     4321 aatgggatag gtcacgttgg tgtagatggg cgcatcgtaa ccgtgcatct gccagtttga
     4381 ggggacgacg gcattatcgg cctcaggaag atcgcactcc agccagcttt ccggcaccgc
     4441 ttctggtgcc ggaaaccagg caaagcgcca ttcgccattc aggctgcgca actgttggga
     4501 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     4561 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     4621 cagtgaatcc gtaatcatgg tccaacaagg tggttctcca agggatactt atagtctcaa
     4681 aacacacaat tactttacag ttagggtgag tttccttttg tgctgtttat aacttcgtat
     4741 aatgtatgct atacgaagtt atagaaatcc aagcctatca tgtaaaatgt agctagtatt
     4801 aaaaagaaca gattatctgt cttttatcgc acattaagcc tctatagtta ctaggaaata
     4861 ttatatgcaa attaaccggg gcaggggagt agccgagctt ctcccacaag tccgtgcgag
     4921 ggggccggcg cgggcctaga gatggcgcgt cggatctgat catatttccc tatagtgagt
     4981 cgtattatct gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaaatct
     5041 tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa aaagtggcta
     5101 agatctacag ctgccttgta agtcattggt cttaaaacgc gcccatgtac tctgcgttga
     5161 taccactgct ttttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
     5221 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
     5281 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
     5341 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
     5401 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
     5461 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
     5521 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
     5581 gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
     5641 gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag
     5701 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag
     5761 ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc
     5821 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc
     5881 cttgctcacc atggtggccg taccgtacgt cgggaggctg aggcaggaga atcgcttgaa
     5941 cccgggaggc ggtcacgaca cctgaaatgg aagaaaaaaa ctttgaacca ctgtctgagg
     6001 cttgagaatg aaccaagatc caaactcaaa aagggcaaat tccaaggaga attacatcaa
     6061 gtgccaagct ggcctaactt cagtctccac ccactcagtg tggggaaact ccatcgcata
     6121 aaacccctcc ccccaaccta aagacgacgt actccaaaag ctcgagaact aatcgaggtg
     6181 cctggacggc gcccggtact ccgtggagtc acatgaagcg acggctgagg acggaaaggc
     6241 ccttttcctt tgtgtgggtg actcacccgc ccgctctccc gagcgccgcg tcctccattt
     6301 tgagctccct gcagcagggc cgggaagcgg ccatctttcc gctcacgcaa ctggtgccga
     6361 ccgggccagc cttgccgccc agggcggggc gatacacggc ggcgcgaggc caggcaccag
     6421 agcaggccgg ccagcttgag actacccccg tccgattctc ggtggccgcg ctcgcaggcc
     6481 ccgcctcgcc gaacatgtgc gctgggacgc acgggccccg tcgccgcccg cggccccaaa
     6541 aaccgaaata ccagtgtgca gatcttggcc cgcatttaca agactatctt gccagaaaaa
     6601 aagcgtcgca gcaggtcatc aaaaatttta aatggctaga gacttatcga aagcagcgag
     6661 acaggcgcga aggtgccacc agattcgcac gcggcggccc cagcgcccag gccaggcctc
     6721 aactcaagca cgaggcgaag gggctcctta agcgcaaggc ctcgaactct cccacccact
     6781 tccaacccga agctcgggat caagaatcac gtactgcagc caggtggaag taattcaagg
     6841 cacgcaaggg ccataacccg taaagaggcc aggcccgcgg gaaccacaca cggcacttac
     6901 ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat
     6961 atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca
     7021 gtttaccccg cgccaccttc tctaggcacc ggatcaattg ccgacccctc cccccaactt
     7081 ctcggggact gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc caattcccac
     7141 tcctttcaag acctagaagg tccattagct gcaaagattc ctctctgttt aaaactttat
     7201 ccatctttgc acccgggccc cctcgagccg gcgccaaagt ggatctctgc tgtccctgta
     7261 ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc
     7321 tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct
     7381 tttaaaattg tggatgaata ctgccatttg tctcaagatt ctgaaacgat aatggtgaat
     7441 atccctgcct aactctattc actatagaaa gtacagcaaa aactattctt aaacctacca
     7501 agcctcctac tatcattatg aataatttta tataccacag ccaatttgtt atgttaaacc
     7561 aattccacaa acttgcccat ttatctaatt ccaataattc ttgttcattc ttttcttgct
     7621 ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta atttctctgt
     7681 cccactccat ccaggtcgtg tgattccaaa tctgttccag agatttatta ctccaactag
     7741 cattccaagg cacagcagtg gtgcaaatga gttttccaga gcaaccccaa atccccagga
     7801 gctgttgatc ctttaggtat ctttccacag ccaggattct tgcctggagc tgcttgatgc
     7861 cccagactgt gagttgcaac agatgctgtt gcgcctcaat agccctcagc aaattgttct
     7921 gctgctgcac tataccagac aataattgtc tggcctgtac cgtcagcgtc attgacgctg
     7981 cgcccatagt gcttcctgct gctcccaaga acccaaggaa caaagctcct attcccactg
     8041 ctcttttttc tctctgcacc actcttctct ttgccttggt gggtgctact cctaatggtt
     8101 caatttttac tactttatat ttatataatt cacttctcca attgtccctc atatctcctc
     8161 ctccaggtct gaagatcagc ggccgcttgc tgtgcggtgg tcttactttt gttttgctct
     8221 tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa
     8281 tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat
     8341 ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag gccaggatta
     8401 actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt atattttttc
     8461 tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata
     8521 ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta
     8581 ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt
     8641 cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag
     8701 agattttcca cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca
     8761 gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt
     8821 tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga gacccgttta
     8881 tgtatcgagc taggcactta aatacaatat ctctgcaatg cggcaattca gtggttcgtc
     8941 caatccatgt cagacccgtc tgttgccttc ctaataaggc acgatcgtac caccttactt
     9001 ccaccaatcg gcatgcacgg tgctttttct ctccttgtaa ggcatgttgc taactcatcg
     9061 ttaccatgtt gcaagactac aagagtattg cataagacta cattaagctt gcagctccag
     9121 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     9181 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     9241 gtgtaaagcc tg
//
