LOCUS       sequence-443624-        9106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral CROP-seq vector with lineage barcode cassette and T7 
            promoter for ID amplification from cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9106)
  AUTHORS   Metzner E, Southard KM, Norman TM
  TITLE     Multiome Perturb-seq unlocks scalable discovery of integrated 
            perturbation effects on the transcriptome and epigenome.
  JOURNAL   Cell Syst. 2025 Jan 15;16(1):101161. doi: 
            10.1016/j.cels.2024.12.002. Epub 2024 Dec 16.
  PUBMED    39689711
REFERENCE   2  (bases 1 to 9106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-01-15"; volume: "16(1):101161. doi"; pages: " 
            10.1016/j.cels.2024.12.002. Epub 2024 Dec 16"
COMMENT     Sequence Label: pEM047 [T7-assisted CROPseq sgRNA expression v...
FEATURES             Location/Qualifiers
     source          1..9106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    5..26
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(143..160)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(314..902)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(394..413)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1073..1864,1865..1933))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1696..1715
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1934..2038)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2064..2519)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2201..2222)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2413..2432
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2661..2677
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2687..2705
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2726..2861
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2880..3001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2891..2910)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2945..2964
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3079..3259
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     4535..4551
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4632..4652)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    4656..4689
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(4896..4914)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(5070..5783,5784..5786,5787..5789))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5073..5783,5784..5786,5787..5789))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5115..5136)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5723..5744
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5786..5795
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5802..6980)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5811..6749
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5834..5854)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7119..7236
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7417..7461)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7646..7879
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8372..8497
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8544..8724
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8725..8951)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(8979..8999)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8979..8997)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9018..9034)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9018..9034)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9031..9053)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9042..9058
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9066..9072,9073..9090,9091..9096))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
       61 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      121 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      181 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      241 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      301 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      361 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      421 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      481 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      541 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      601 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      661 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      721 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      781 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      841 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      901 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      961 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1021 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1081 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1141 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1201 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1261 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1321 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1381 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1441 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1501 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1561 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1621 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1681 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     1741 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1801 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     1861 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     1921 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     1981 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2041 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     2101 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     2161 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     2221 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     2281 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     2341 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     2401 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     2461 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     2521 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2581 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     2641 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
     2701 atagggcgaa ttgggtacgt ccctaggcct ccaaaaaagc ctcctcacta cttctggaat
     2761 agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg
     2821 ggcggagaat gggcggaact gggcggagtt aggggcggga tagctagagc cagacatgat
     2881 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     2941 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     3001 tcctctcact ctctgatatt catttctttg caagttataa atactgaata ataagatgac
     3061 atgaactact atacgtactg ctagagattt tccacactga ctaaaagggt ctgagggatc
     3121 tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc
     3181 tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct
     3241 ggtctaacca gagagaccca gtacaagcaa aaagcagtgt ctgtcagcta tgcactggcg
     3301 attgtggatc ctgtcagttg tcgggttgtc gactcgtgag tctggacctt tatcgcaaaa
     3361 aaagcaccga ctcggtgcca ctttttcaag ttgataacgg actagcctta tttaaacttg
     3421 ctatgctgtt tccagcttag ctcttaaacc accatcgtct gctcatccat gacctgacca
     3481 tgcagaggat gatgctcgtg acggttaacg cctcgaatca gcaacggctt gccgttcagc
     3541 agcagcagac cattttcaat ccgcacctcg cggaaaccga catcgcaggc ttctgcttca
     3601 atcagcgtgc cgtcggcggt gtgcagttca accaccgcac gatagagatt cgggatttcg
     3661 gcgctccaca gtttcgggtt ttcgacgttc agacgtagtg tgacgcgatc ggcataacca
     3721 ccacgctcat cgataatttc accgccgaaa ggcgcggtgc cgctggcgac gtgcgtttca
     3781 ccctgccata aagaaactgt tacccgtagg tagtcacgca actcgccgca catctgaact
     3841 tcagcctcca gtacagcgcg gctgaaatca tcattaaagc gagtggcaac atggaaatcg
     3901 ctgatttgtg tagtcggttt atgcagcaac gtgatgtcac ggaaaatgcc gctcatccgc
     3961 cacatatcct gatcttccag ataactgccg tcactccagc gcagcaccat caccgcgagg
     4021 cggttttctc cggcgcgtaa aaatgcgctc aggtcaaatt cagacggcaa acgactgtcc
     4081 tggccgtaac cgacccagcg cccgttgcac cacagatgaa acgccgattt aatgccatca
     4141 aaaataattc gcgtctggcc ttcctgtagc cagctttcat caacattaaa tgtgagcgag
     4201 taacaacccg tcggattctc cgtgggaaca aacggcggat tgaccgtaat gggataggtc
     4261 acgttggtgt agatgggcgc atcgtaaccg tgcatctgcc agtttgaggg gacgacggca
     4321 ttatcggcct caggaagatc gcactccagc cagctttccg gcaccgcttc tggtgccgga
     4381 aaccaggcaa agcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     4441 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     4501 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaatccgta
     4561 atcatggtcc aacaaggtgg ttctccaagg gatacttata gtctcaaaac acacaattac
     4621 tttacagtta gggtgagttt ccttttgtgc tgtttataac ttcgtataat gtatgctata
     4681 cgaagttata gaaatccaag cctatcatgt aaaatgtagc tagtattaaa aagaacagat
     4741 tatctgtctt ttatcgcaca ttaagcctct atagttacta ggaaatatta tatgcaaatt
     4801 aaccggggca ggggagtagc cgagcttctc ccacaagtcc gtgcgagggg gccggcgcgg
     4861 gcctagagat ggcgcgtcgg atctgatcat atttccctat agtgagtcgt attatctggg
     4921 tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaaatcttgt cttcgttggg
     4981 agtgaattag cccttccagt cccccctttt cttttaaaaa gtggctaaga tctacagctg
     5041 ccttgtaagt cattggtctt aaaacgcgtt tacttgtaca gctcgtccat gccgagagtg
     5101 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
     5161 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
     5221 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
     5281 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
     5341 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
     5401 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
     5461 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
     5521 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac
     5581 tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
     5641 gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac
     5701 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
     5761 gtgaacagct cctcgccctt gctcaccatg gtggccgtac gtcacgacac ctgaaatgga
     5821 agaaaaaaac tttgaaccac tgtctgaggc ttgagaatga accaagatcc aaactcaaaa
     5881 agggcaaatt ccaaggagaa ttacatcaag tgccaagctg gcctaacttc agtctccacc
     5941 cactcagtgt ggggaaactc catcgcataa aacccctccc cccaacctaa agacgacgta
     6001 ctccaaaagc tcgagaacta atcgaggtgc ctggacggcg cccggtactc cgtggagtca
     6061 catgaagcga cggctgagga cggaaaggcc cttttccttt gtgtgggtga ctcacccgcc
     6121 cgctctcccg agcgccgcgt cctccatttt gagctccctg cagcagggcc gggaagcggc
     6181 catctttccg ctcacgcaac tggtgccgac cgggccagcc ttgccgccca gggcggggcg
     6241 atacacggcg gcgcgaggcc aggcaccaga gcaggccggc cagcttgaga ctacccccgt
     6301 ccgattctcg gtggccgcgc tcgcaggccc cgcctcgccg aacatgtgcg ctgggacgca
     6361 cgggccccgt cgccgcccgc ggccccaaaa accgaaatac cagtgtgcag atcttggccc
     6421 gcatttacaa gactatcttg ccagaaaaaa agcgtcgcag caggtcatca aaaattttaa
     6481 atggctagag acttatcgaa agcagcgaga caggcgcgaa ggtgccacca gattcgcacg
     6541 cggcggcccc agcgcccagg ccaggcctca actcaagcac gaggcgaagg ggctccttaa
     6601 gcgcaaggcc tcgaactctc ccacccactt ccaacccgaa gctcgggatc aagaatcacg
     6661 tactgcagcc aggtggaagt aattcaaggc acgcaagggc cataacccgt aaagaggcca
     6721 ggcccgcggg aaccacacac ggcacttacc tgtgttctgg cggcaaaccc gttgcgaaaa
     6781 agaacgttca cggcgactac tgcacttata tacggttctc ccccaccctc gggaaaaagg
     6841 cggagccagt acacgacatc actttcccag tttaccccgc gccaccttct ctaggcaccg
     6901 gatcaattgc cgacccctcc ccccaacttc tcggggactg tgggcgatgt gcgctctgcc
     6961 cactgacggg caccggagcc aattcccact cctttcaaga cctagaaggt ccattagctg
     7021 caaagattcc tctctgttta aaactttatc catctttgca cccgggcccc ctcgagccgg
     7081 cgccaaagtg gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt
     7141 tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct
     7201 gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt
     7261 ctcaagattc tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag
     7321 tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga ataattttat
     7381 ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc
     7441 caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat
     7501 taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat
     7561 ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag
     7621 ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc
     7681 caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg
     7741 cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct
     7801 ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa
     7861 cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt
     7921 tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt tatataattc
     7981 acttctccaa ttgtccctca tatctcctcc tccaggtctg aagatcagcg gccgcttgct
     8041 gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt
     8101 cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc
     8161 taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc
     8221 cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc
     8281 atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc
     8341 atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct
     8401 agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg
     8461 tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt
     8521 tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag
     8581 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     8641 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     8701 gatctggtct aaccagagag acccgtttat gtatcgagct aggcacttaa atacaatatc
     8761 tctgcaatgc ggcaattcag tggttcgtcc aatccatgtc agacccgtct gttgccttcc
     8821 taataaggca cgatcgtacc accttacttc caccaatcgg catgcacggt gctttttctc
     8881 tccttgtaag gcatgttgct aactcatcgt taccatgttg caagactaca agagtattgc
     8941 ataagactac attaagcttg cagctccagc ttttgttccc tttagtgagg gttaattgcg
     9001 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     9061 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtg
//
