LOCUS       sequence-443629-        9636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses autophagy flux LC3B fluorescence reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9636)
  AUTHORS   Ortiz ANR, Zhang L, Ash PEA, Basu A, Puri S, van der Spek SJF, Wang 
            Z, Dorrian L, Emili A, Wolozin B
  TITLE     Proximity labeling reveals dynamic changes in the SQSTM1 protein 
            network.
  JOURNAL   bioRxiv [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324.
  PUBMED    38168279
REFERENCE   2  (bases 1 to 9636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324."
COMMENT     Sequence Label: pRRL-SV40-Puro-CMV-mCherry-EGFP-LC3B
FEATURES             Location/Qualifiers
     source          1..9636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    84..317
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    810..935
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             982..1162
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1163..1389)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1417..1437)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1417..1435)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1456..1472)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1456..1472)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1469..1491)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1480..1496
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1504..1510,1511..1528,1529..1534))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1549..1570
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1687..1704)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1858..2446)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1938..1957)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2617..3408,3409..3477))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3240..3259
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3478..3582)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3608..4063)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3745..3766)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3957..3976
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4190..4212
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4204..4221
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4205..4221
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4231..4250
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4231..4249
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4270..4405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4445..4566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4456..4475)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4510..4529
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4638..4871
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4962..5550
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5068..5079
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5477..5497
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5577..5951)
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
     CDS             complement(join(5979..6689,6690..6692,6693..6695))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5979..6689,6690..6692,6693..6695))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6021..6042)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6370..6389
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6629..6650
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6692..6701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6710..6734)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(6762..7469)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7037..7056)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7306..7324
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      7466..7475
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7549..7573)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(7570..7773)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7599..7619)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7774..8077
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(8153..8752)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8236..8256)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8733..8752
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8762..9091)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8776..8911
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9076..9096
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    9108..9224
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(9491..9535)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gttccagaga tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt
       61 ttccagagca accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca
      121 ggattcttgc ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg
      181 cctcaatagc cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg
      241 cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc
      301 caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg
      361 ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta tataattcac
      421 ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc cgcttgctgt
      481 gcggtggtct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct
      541 tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta
      601 agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct
      661 tctgatgttt ctaacaggcc aggattaact gcgaatcgtt ctagctccct gcttgcccat
      721 actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat tttttcccat
      781 cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct ctccttctag
      841 cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg
      901 cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc tttcgctttc
      961 aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     1021 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     1081 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     1141 tctggtctaa ccagagagac ccgtttatgt atcgagctag gcacttaaat acaatatctc
     1201 tgcaatgcgg caattcagtg gttcgtccaa tccatgtcag acccgtctgt tgccttccta
     1261 ataaggcacg atcgtaccac cttacttcca ccaatcggca tgcacggtgc tttttctctc
     1321 cttgtaaggc atgttgctaa ctcatcgtta ccatgttgca agactacaag agtattgcat
     1381 aagactacat taagcttgca gctccagctt ttgttccctt tagtgagggt taattgcgcg
     1441 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     1501 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     1561 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     1621 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     1681 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     1741 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1801 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1861 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1921 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1981 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2041 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2101 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2161 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2221 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2281 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2341 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2401 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2461 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2521 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     2581 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     2641 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     2701 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     2761 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     2821 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2881 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     2941 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3001 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3061 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3121 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3181 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3241 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3301 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3361 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3421 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3481 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3541 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3601 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     3661 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     3721 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     3781 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     3841 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     3901 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     3961 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     4021 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     4081 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     4141 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     4201 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt
     4261 acgtccctag gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg
     4321 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg
     4381 aactgggcgg agttaggggc gggatgggcg gagttagggg cgggatagct agagccagac
     4441 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     4501 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     4561 caagttcctc tcactctctg atattcattt ctttgcaagt tataaatact gaataataag
     4621 atgacatgaa ctactactgc tagagatttt ccacactgac taaaagggtc tgagggatct
     4681 ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct
     4741 ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg
     4801 gtctaaccag agagacccag tacaagcaaa aagcagatct tgtcttcgtt gggagtgaat
     4861 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
     4921 agtcattggt cttaaaggta ccgagctcga attaattcca ggcggggagg cggcccaaag
     4981 ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag
     5041 caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt
     5101 cccgcgcaga atccaggtgg caacacaggc gagcagccat ggaaaggacg tcagcttccc
     5161 cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag
     5221 gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag
     5281 gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca
     5341 caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga
     5401 tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat
     5461 taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct
     5521 ttcacaaatt ttgtaatcca gaggttgatt gtcgacgaat tctggtgctc gagttacact
     5581 gacaatttca tcccgaacgt ctcctgggag gcatagacca tgtacaggaa tccatcttca
     5641 tctttctcac tctcatacac ctctgagatt ggtgtggaga cgctgaccat gctgtgtccg
     5701 ttcaccaaca ggaagaaggc ctgattagca ttgagctgta agcgccttct aattatcttg
     5761 atgagctcac tcatgttgac atggtcaggt acaaggaact ttgttttatc cagaacagga
     5821 agctgcttct cacccttgta tcgttctatt atcaccggga ttttggttgg atgctgctct
     5881 cgaataagtc ggacatcttc tactctttgt tcgaaggtgc ggcgctgctt gaaggtcttc
     5941 tccgacggca tgaattcggt accggatctg agtccggact tgtacagctc gtccatgccg
     6001 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
     6061 gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg
     6121 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg
     6181 atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata
     6241 tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
     6301 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
     6361 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
     6421 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
     6481 aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
     6541 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
     6601 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
     6661 accccggtga acagctcctc gcccttgctc accatggtgg cgaccggaaa gcctgctttt
     6721 ttgtacaaac ttgttcctgg gccagcggat ctgagtccgg acttgtacag ctcgtccatg
     6781 ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg
     6841 ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg cgccgggcag ctgcacgggc
     6901 ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc
     6961 agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag
     7021 gcctcccagc ccatggtctt cttctgcatt acggggccgt cggaggggaa gttggtgccg
     7081 cgcagcttca ccttgtagat gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc
     7141 accacgccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac
     7201 agcttcaagt agtcggggat gtcggcgggg tgcttcacgt aggccttgga gccgtacatg
     7261 aactgagggg acaggatgtc ccaggcgaag ggcagggggc cacccttggt caccttcagc
     7321 ttggcggtct gggtgccctc gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg
     7381 tggccgttca cggagccctc catgtgcacc ttgaagcgca tgaactcctt gatgatggcc
     7441 atgttatcct cctcgccctt gctcaccatg gtggcgaccg gtagcgctag cggtctagag
     7501 tcggtgtctt ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt
     7561 cactaaacga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     7621 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     7681 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     7741 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
     7801 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     7861 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     7921 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     7981 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     8041 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactcccaag cttatcgatg
     8101 catggggtcg tgcgctcctt tcggtcgggc gctgcgggtc gtggggcggg cgtcaggcac
     8161 cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg tcggcggtga
     8221 cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc tccaggaagg
     8281 cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg ctgcccagac
     8341 ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg ggctccttgg
     8401 gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg gaaccgctca
     8461 actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg ctctccggcg
     8521 tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc
     8581 cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg cggtccggat
     8641 cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc
     8701 tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc atggtaagct
     8761 ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg
     8821 ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat
     8881 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg
     8941 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     9001 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     9061 ctggggactt tccacaccct aactgacaca cattccacag ggtcgataaa attttgaatt
     9121 ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc
     9181 tttcccctgc actgtacccc ccaatccccc cttttctttt aaaagttaac cgataccgtc
     9241 gagatccgtt cactaatcga atggatctgt ctctgtctct ctctccacct tcttcttcta
     9301 ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg
     9361 aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta
     9421 ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa
     9481 accaattcca caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt
     9541 gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc
     9601 tgtcccactc catccaggtc gtgtgattcc aaatct
//
