LOCUS       sequence-443630-       11220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG-TurboID-dUBA-SQSTM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11220)
  AUTHORS   Ortiz ANR, Zhang L, Ash PEA, Basu A, Puri S, van der Spek SJF, Wang 
            Z, Dorrian L, Emili A, Wolozin B
  TITLE     Proximity labeling reveals dynamic changes in the SQSTM1 protein 
            network.
  JOURNAL   bioRxiv [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324.
  PUBMED    38168279
REFERENCE   2  (bases 1 to 11220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324."
COMMENT     Sequence Label: pHR-SFFV-FLAG-TurboID-dUBA-SQSTM1
FEATURES             Location/Qualifiers
     source          1..11220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(291..310)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(524..543)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     643..665
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(703..721)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        789..893
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(894..962,963..1754)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1925..2513
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2414..2433
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2667..2684
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2754..2774)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2759..3088
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2939..3074
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4222..4287
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4417..4437
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4862..4996
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4899..4918)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4953..4972
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5164..5797
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5844..5969
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6466..6699
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6884..6928
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7213..7330
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7483..7890
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      7931..7940
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7940..7963
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7964..8920
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    10247..10835
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10300..10320)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10718..10729)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10838..10854)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10839..10855)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10945..11178
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(join(11203..11220,1))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(join(11204..11220,1))
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat
       61 gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta
      121 aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc
      181 gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc
      241 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
      301 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
      361 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
      421 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
      481 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
      541 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
      601 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
      661 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
      721 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
      781 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      841 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      901 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      961 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1021 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1081 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1141 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1201 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1261 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1321 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1381 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1441 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1501 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1561 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1621 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1681 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1741 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1801 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1861 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1921 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1981 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2041 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2101 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2161 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2221 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2281 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2341 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2401 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2461 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2521 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2581 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2641 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2701 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     2761 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     2821 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     2881 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     2941 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3001 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3061 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     3121 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     3181 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     3241 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     3301 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     3361 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     3421 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     3481 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
     3541 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     3601 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
     3661 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
     3721 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     3781 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
     3841 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
     3901 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
     3961 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
     4021 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
     4081 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
     4141 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
     4201 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     4261 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
     4321 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     4381 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     4441 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     4501 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     4561 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
     4621 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
     4681 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
     4741 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     4801 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     4861 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4921 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4981 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     5041 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
     5101 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     5161 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
     5221 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     5281 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     5341 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     5401 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     5461 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     5521 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     5581 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     5641 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     5701 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     5761 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     5821 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     5881 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     5941 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     6001 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     6061 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     6121 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     6181 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     6241 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     6301 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     6361 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     6421 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     6481 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     6541 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     6601 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     6661 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     6721 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     6781 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     6841 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     6901 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     6961 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     7021 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     7081 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     7141 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc aaatggcagt
     7201 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     7261 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     7321 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggatcg ataagcttga
     7381 tatcgaattc ctgcagcccc gataaaataa aagattttat ttagtctcca gaaaaagggg
     7441 ggaatgaaag accccacctg taggtttggc aagctagctg cagtaacgcc attttgcaag
     7501 gcatggaaaa ataccaaacc aagaatagag aagttcagat caagggcggg tacatgaaaa
     7561 tagctaacgt tgggccaaac aggatatctg cggtgagcag tttcggcccc ggcccggggc
     7621 caagaacaga tggtcaccgc agtttcggcc ccggcccgag gccaagaaca gatggtcccc
     7681 agatatggcc caaccctcag cagtttctta agacccatca gatgtttcca ggctccccca
     7741 aggacctgaa atgaccctgc gccttatttg aattaaccaa tcagcctgct tctcgcttct
     7801 gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc acaacccctc actcggcgcg
     7861 ccagtcctcc gacagactga gtcgcccggg ggggatctgg agctctcgag aattctcacg
     7921 cgtaccggta gccaccatgg actacaagga tgacgacgat aagaaagaca atactgtgcc
     7981 tctgaagctg atcgctctcc tggctaatgg cgagttccat agtggcgaac agctgggaga
     8041 aaccctgggc atgtccaggg ccgctatcaa caagcacatt cagactctgc gcgactgggg
     8101 cgtggacgtg ttcaccgtgc ccggaaaggg ctactctctg cccgagccta tcccgctgct
     8161 gaacgctaaa cagattctgg gacagctgga cggcgggagc gtggcagtcc tgcctgtggt
     8221 cgactccacc aatcagtacc tgctggatcg aatcggcgag ctgaagagtg gggatgcttg
     8281 cattgcagaa tatcagcagg cagggagagg aagcagaggg aggaaatggt tctctccttt
     8341 tggagctaac ctgtacctga gtatgttttg gcgcctgaag cggggaccag cagcaatcgg
     8401 cctgggcccg gtcatcggaa ttgtcatggc agaagcgctg cgaaagctgg gagcagacaa
     8461 ggtgcgagtc aaatggccca atgacctgta tctgcaggat agaaagctgg caggcatcct
     8521 ggtggagctg gccggaataa caggcgatgc tgcacagatc gtcattggcg ccgggattaa
     8581 cgtggctatg aggcgcgtgg aggaaagcgt ggtcaatcag ggctggatca cactgcagga
     8641 agcagggatt aacctggaca ggaatactct ggccgctacg ctgatccgag agctgcgggc
     8701 agccctggaa ctgttcgagc aggaaggcct ggctccatat ctgccacggt gggagaagct
     8761 ggataacttc atcaatagac ccgtgaagct gatcattggg gacaaagaga ttttcgggat
     8821 tagccggggg attgataaac agggagccct gctgctggaa caggacggag ttatcaaacc
     8881 ctggatgggc ggagaaatca gtctgcggtc tgccgaaaag tccggactca gatctggtgg
     8941 cagtggatct cgagtcatgg cgtcgctcac cgtgaaggcc taccttctgg gcaaggagga
     9001 cgcggcgcgc gagattcgcc gcttcagctt ctgctgcagc cccgagcctg aggcggaagc
     9061 cgaggctgcg gcgggtccgg gaccctgcga gcggctgctg agccgggtgg ccgccctgtt
     9121 ccccgcgctg cggcctggcg gcttccaggc gcactaccgc gatgaggacg gggacttggt
     9181 tgccttttcc agtgacgagg aattgacaat ggccatgtcc tacgtgaagg atgacatctt
     9241 ccgaatctac attaaagaga aaaaagagtg ccggcgggac caccgcccac cgtgtgctca
     9301 ggaggcgccc cgcaacatgg tgcaccccaa tgtgatctgc gatggctgca atgggcctgt
     9361 ggtaggaacc cgctacaagt gcagcgtctg cccagactac gacttgtgta gcgtctgcga
     9421 gggaaagggc ttgcaccggg ggcacaccaa gctcgcattc cccagcccct tcgggcacct
     9481 gtctgagggc ttctcgcaca gccgctggct ccggaaggtg aaacacggac acttcgggtg
     9541 gccaggatgg gaaatgggtc caccaggaaa ctggagccca cgtcctcctc gtgcagggga
     9601 ggcccgccct ggccccacgg cagaatcagc ttctggtcca tcggaggatc cgagtgtgaa
     9661 tttcctgaag aacgttgggg agagtgtggc agctgccctt agccctctgg gcattgaagt
     9721 tgatatcgat gtggagcacg gagggaaaag aagccgcctg acccccgtct ctccagagag
     9781 ttccagcaca gaggagaaga gcagctcaca gccaagcagc tgctgctctg atcccagcaa
     9841 gccgggtggg aatgttgagg gcgccacgca gtctctggcg gagcagatga gaaagatcgc
     9901 cttggagtcc gaggggcgcc ctgaggaaca gatggagtcg gataactgtt caggaggaga
     9961 tgatgactgg acccatctgt cttcaaaaga agtggacccg tctacaggtg aactccagtc
    10021 cctacagatg ccagaatccg aagggccaag ctctctggac ccctcccagg agggacccac
    10081 agggctgaag gaagctgcct tgtactaagg atccaccgga tctagataac tgattaagat
    10141 ccttgacttg cggccgcaac tcccacctgc aacatgcgtg actgactgag gccgcgactc
    10201 tagagtcgac ctgcaggcat gcaagcttga tatcaagctt atcgataatc aacctctgga
    10261 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
    10321 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
    10381 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
    10441 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
    10501 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
    10561 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
    10621 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
    10681 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
    10741 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
    10801 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagggaa
    10861 ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
    10921 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
    10981 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
    11041 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
    11101 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
    11161 tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa
//
