LOCUS       sequence-443631-       11040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG-TurboID-dPB1-SQSTM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11040)
  AUTHORS   Ortiz ANR, Zhang L, Ash PEA, Basu A, Puri S, van der Spek SJF, Wang 
            Z, Dorrian L, Emili A, Wolozin B
  TITLE     Proximity labeling reveals dynamic changes in the SQSTM1 protein 
            network.
  JOURNAL   bioRxiv [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324.
  PUBMED    38168279
REFERENCE   2  (bases 1 to 11040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324."
COMMENT     Sequence Label: pHR-SFFV-FLAG-TurboID-dPB1-SQSTM1
FEATURES             Location/Qualifiers
     source          1..11040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        90..108
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     90..107
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             133..366
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     456..472
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     457..473
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    476..1064
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             582..593
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     991..1011
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2211..3167)
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             complement(3168..3191)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      3191..3200
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3241..3648)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    3801..3918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4203..4247)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4432..4665
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5162..5287
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5334..5967
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6135..6269
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6159..6178)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6213..6232
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6694..6714)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6844..6909
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(8043..8372)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8057..8192
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8357..8377
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(8447..8464)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8618..9206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8698..8717)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9377..10168,10169..10237))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10238..10342)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10410..10428
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10466..10488)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10588..10607
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10821..10840
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aagccagatg ctacacaatt aggcttgtac atattgtcgt tagaacgcgg ctacaattaa
       61 tacataacct tatgtatcat acacatacga tttaggtgac actatagaat acacggaatt
      121 aattctagat gctgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
      181 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
      241 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
      301 accagagaga cccagtacaa gcaaaaagca gatcttgtct tcgttgggag tgaattagcc
      361 cttccagtcc ccccttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca
      421 ttggtcttaa aggtaccgag ctcgaattaa ttccctcgag gtcgacggta tcgatgcggg
      481 gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc
      541 cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg
      601 tagcagaagg acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccaaggaaag
      661 gacgatgatt tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc
      721 cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg
      781 aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca
      841 gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga
      901 gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca
      961 tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga
     1021 ataccagtca atctttcaca aattttgtaa tccagaggtt gattatcgat aagcttgata
     1081 tcaagcttgc atgcctgcag gtcgactcta gagtcgcggc ctcagtcagt cacgcatgtt
     1141 gcaggtggga gttgcggccg caagtcaagg atcttaatca gttatctaga tccggtggat
     1201 ccttacaacg gcgggggatg ctttgaatac tggatggtgt ccagagccgc tccgatgtca
     1261 tagttcttgg tctgcaggag cctggtgagc cagccgcctt catcagagaa gcccatggac
     1321 agcatctggg agagggactc aatcagccgc gggtcagcct ctggcgggag atgtgggtac
     1381 aaggcagctt ccttcagccc tgtgggtccc tcctgggagg ggtccagaga gcttggccct
     1441 tcggattctg gcatctgtag ggactggagt tcacctgtag acgggtccac ttcttttgaa
     1501 gacagatggg tccagtcatc atctcctcct gaacagttat ccgactccat ctgttcctca
     1561 gggcgcccct cggactccaa ggcgatcttt ctcatctgct ccgccagaga ctgcgtggcg
     1621 ccctcaacat tcccacccgg cttgctggga tcagagcagc agctgcttgg ctgtgagctg
     1681 ctcttctcct ctgtgctgga actctctgga gagacggggg tcaggcggct tcttttccct
     1741 ccgtgctcca catcgatatc aacttcaatg cccagagggc taagggcagc tgccacactc
     1801 tccccaacgt tcttcaggaa attcacactc ggatcctccg atggaccaga agctgattct
     1861 gccgtggggc cagggcgggc ctcccctgca cgaggaggac gtgggctcca gtttcctggt
     1921 ggacccattt cccatcctgg ccacccgaag tgtccgtgtt tcaccttccg gagccagcgg
     1981 ctgtgcgaga agccctcaga caggtgcccg aaggggctgg ggaatgcgag cttggtgtgc
     2041 ccccggtgca agccctttcc ctcgcagacg ctacacaagt cgtagtctgg gcagacgctg
     2101 cacttgtagc gggttcctac cacaggccca ttgcagccat cgcagatcac attggggtgc
     2161 accatgttgc gcatgactcg agatccactg ccaccagatc tgagtccgga cttttcggca
     2221 gaccgcagac tgatttctcc gcccatccag ggtttgataa ctccgtcctg ttccagcagc
     2281 agggctccct gtttatcaat cccccggcta atcccgaaaa tctctttgtc cccaatgatc
     2341 agcttcacgg gtctattgat gaagttatcc agcttctccc accgtggcag atatggagcc
     2401 aggccttcct gctcgaacag ttccagggct gcccgcagct ctcggatcag cgtagcggcc
     2461 agagtattcc tgtccaggtt aatccctgct tcctgcagtg tgatccagcc ctgattgacc
     2521 acgctttcct ccacgcgcct catagccacg ttaatcccgg cgccaatgac gatctgtgca
     2581 gcatcgcctg ttattccggc cagctccacc aggatgcctg ccagctttct atcctgcaga
     2641 tacaggtcat tgggccattt gactcgcacc ttgtctgctc ccagctttcg cagcgcttct
     2701 gccatgacaa ttccgatgac cgggcccagg ccgattgctg ctggtccccg cttcaggcgc
     2761 caaaacatac tcaggtacag gttagctcca aaaggagaga accatttcct ccctctgctt
     2821 cctctccctg cctgctgata ttctgcaatg caagcatccc cactcttcag ctcgccgatt
     2881 cgatccagca ggtactgatt ggtggagtcg accacaggca ggactgccac gctcccgccg
     2941 tccagctgtc ccagaatctg tttagcgttc agcagcggga taggctcggg cagagagtag
     3001 ccctttccgg gcacggtgaa cacgtccacg ccccagtcgc gcagagtctg aatgtgcttg
     3061 ttgatagcgg ccctggacat gcccagggtt tctcccagct gttcgccact atggaactcg
     3121 ccattagcca ggagagcgat cagcttcaga ggcacagtat tgtctttctt atcgtcgtca
     3181 tccttgtagt ccatggtggc taccggtacg cgtgagaatt ctcgagagct ccagatcccc
     3241 cccgggcgac tcagtctgtc ggaggactgg cgcgccgagt gaggggttgt gagctctttt
     3301 atagagctcg ggaagcagaa gcgcgcgaac agaagcgaga agcaggctga ttggttaatt
     3361 caaataaggc gcagggtcat ttcaggtcct tgggggagcc tggaaacatc tgatgggtct
     3421 taagaaactg ctgagggttg ggccatatct ggggaccatc tgttcttggc ctcgggccgg
     3481 ggccgaaact gcggtgacca tctgttcttg gccccgggcc ggggccgaaa ctgctcaccg
     3541 cagatatcct gtttggccca acgttagcta ttttcatgta cccgcccttg atctgaactt
     3601 ctctattctt ggtttggtat ttttccatgc cttgcaaaat ggcgttactg cagctagctt
     3661 gccaaaccta caggtggggt ctttcattcc cccctttttc tggagactaa ataaaatctt
     3721 ttattttatc ggggctgcag gaattcgata tcaagcttat cgatccaaac tggatctctg
     3781 ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta
     3841 gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc
     3901 cccccttttc ttttaaaatt gtggatgaat actgccattt ggcgataccg tcgagatccg
     3961 ttcactaatc gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg
     4021 ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc
     4081 tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct
     4141 cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc
     4201 cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt
     4261 tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac
     4321 tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc
     4381 caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt
     4441 tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag
     4501 actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc
     4561 tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc
     4621 atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt
     4681 ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt
     4741 tttactactt tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca
     4801 ggtctgaaga tcagcggccg gccgcttgct gtgcggtggt cttacttttg ttttgctctt
     4861 cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat
     4921 agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg
     4981 gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa
     5041 ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct
     5101 ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac
     5161 tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac
     5221 tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc
     5281 gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga
     5341 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
     5401 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     5461 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca
     5521 ggcaaaaagc agctgcttat atgcaggatc tgagggctcg ccactcccca gtcccgccca
     5581 ggccacgcct ccctggaaag tccccagcgg aaagtccctt gtagcaagct cgatatcagc
     5641 agttcttgaa gtactccgga tgcagctctc gggccacgtg atgaaatgct aggcggctgt
     5701 caaacctcca ctctaacact tctctctccg ggtcatccat cccatgcagg ctcacagggt
     5761 gtaacaagct ggtgttctct cctttattgg cctcttctac cttatctggc tcaactggta
     5821 ctagcttgta gcaccatcca aaggtcagtg gatatctgac ccctggccct ggtgtgtagt
     5881 tctgctaatc agggaagtag ccttgtgtgt ggtagatcca cagatcaagg atatcttgtc
     5941 ttctttggga gtgaattagc ccttccaact actaagtttg tagtacatat ttaacaaata
     6001 caatttcttt aaaatgaaaa taattcagag gaatcacagg tttagagtaa atgaaaccac
     6061 aggtaattgg cagtggtaat agggtatggg gtgggaagtt tgggatgatt ttggttagct
     6121 tgagttatcc agttgatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     6181 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     6241 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     6301 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     6361 tggctgatta tgatctctag tcaaggcact atacatcaaa tattccttat taaccccttt
     6421 acaaattaaa aagctaaagg tacacaattt ttgagcatag ttattaatag cagacactct
     6481 atgcctgtgt ggagtaagaa aaaacagtat gttatgatta taactgttat gcctacttat
     6541 aaaggttaca gaatattttt ccataatttt cttgtatagc agtgcagctt tttcctttgt
     6601 ggtgtaaata gcaaagcaag caagagttct attactaaac acagcatgac tcaaaaaact
     6661 tagcaattct gaaggaaagt ccttggggtc ttctaccttt ctcttctttt ttggaggagt
     6721 agaatgttga gagtcagcag tagcctcatc atcactagat ggcatttctt ctgagcaaaa
     6781 caggttttcc tcattaaagg cattccacca ctgctcccat tcatcagttc cataggttgg
     6841 aatctaaaat acacaaacaa ttagaatcag tagtttaaca cattatacac ttaaaaattt
     6901 tatatttacc ttagagcttt aaatctctgt aggtagtttg tccaattatg tcacaccaca
     6961 gaagtaaggt tccttcacaa agatccgggg cccactcata aatccagttg ccgccacggt
     7021 agccaatcac cgtatcgtat aaatcatcgt cggtacgttc ggcatcgctc atcacaatac
     7081 gtgcctggac gtcgaggatt tcgcgtgggt caatgccgcg ccagatccac atcagacggt
     7141 taatcatgcg ataccagtga gggatggttt taccatcaag ggccgactgc acaggcggtt
     7201 gtgcgccgtg attaaagcgg cggactagcg tcgaggtttc aggatgttta aagcggggtt
     7261 tgaacagggt ttcgctcagg tttgcctgtg tcatggatgc agcctccaga atacttactg
     7321 gaaactattg taacccgcct gaagttaaaa agaacaacgc ccggcagtgc caggcgttga
     7381 aaagattagc gaccggagat tggcgggacg aatacgacgc ccatatccca cggctgttca
     7441 atccaggtat cttgcgggat atcaacaaca tagtcatcaa ccagcggacg accagccggt
     7501 tttgcgaaga tggtgacaaa gtgcgctttt ggatacattt cacgaatcgc aaccgcagta
     7561 ccaccggtat ccaccaggtc atcaataacg atgaagcctt cgccatcgcc ttctgcgcgt
     7621 ttcagcactt taagctcgcg ctggttgtcg tgatcgtagc tggaaataca aacggtatcg
     7681 acatgacgaa tacccagttc acgcgccagt aacgcacccg gtacagaccg ccacggctta
     7741 cggcaataat gcctttccat tgttcagaag gcatcagtcg gcttgcgagt ttacgtgcat
     7801 ggatctgcaa catgtcccag gtgacgatgt atttttcgct catgtgaagt gtcccagcct
     7861 gtttatctac ggcttaaaaa gtgttcgagg ggaaaatagg ttgcgcgaga ttatagagat
     7921 ctggcgcact aaaaaccagt atttcacatg agtccgcgtc tttttacgca ctgcctctcc
     7981 ctgacgcggg ataaagtggt attctcaaac atatctcgca agcctgtctt gtgtccaagc
     8041 tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     8101 gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     8161 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     8221 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     8281 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     8341 cctggggact ttccacaccc taactgacac acattccaca gctgcattaa tgaatcggcc
     8401 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8461 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8521 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8581 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8641 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8701 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8761 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8821 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8881 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8941 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9001 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     9061 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9121 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9181 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9241 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9301 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9361 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9421 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9481 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9541 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9601 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9661 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9721 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9781 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9841 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9901 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9961 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10021 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10081 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10141 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10201 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10261 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10321 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    10381 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
    10441 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    10501 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    10561 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
    10621 attcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
    10681 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
    10741 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
    10801 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
    10861 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctggctagc gatgaccctg
    10921 ctgattggtt cgctgaccat ttccgggtgc gggacggcgt taccagaaac tcagaaggtt
    10981 cgtccaacca aaccgactct gacggcagtt tacgagagag atgatagggt ctgcttcagt
//
