LOCUS       sequence-443633-       10099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Flag-tagged TurboID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10099)
  AUTHORS   Ortiz ANR, Zhang L, Ash PEA, Basu A, Puri S, van der Spek SJF, Wang 
            Z, Dorrian L, Emili A, Wolozin B
  TITLE     Proximity labeling reveals dynamic changes in the SQSTM1 protein 
            network.
  JOURNAL   bioRxiv [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324.
  PUBMED    38168279
REFERENCE   2  (bases 1 to 10099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324."
COMMENT     Sequence Label: pHR-SFFV-FLAG-TurboID
FEATURES             Location/Qualifiers
     source          1..10099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     82..104
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(142..160)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        228..332
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(333..401,402..1193)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1364..1952
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1853..1872
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2106..2123
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2193..2213)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2198..2527
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2378..2513
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          3661..3726
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3856..3876
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4301..4435
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4338..4357)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4392..4411
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4603..5236
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5283..5408
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5905..6138
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6323..6367
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6652..6769
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6922..7329
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      7377..7386
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7386..7409
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7410..8366
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    8367..8432
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    8565..9153
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8618..8638)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9036..9047)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9156..9172)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9157..9173)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9263..9496
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(9521..9539)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9522..9539)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9829..9848)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10062..10081)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
       61 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
      121 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
      181 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      241 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      301 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      361 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
      421 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
      481 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
      541 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
      601 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
      661 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
      721 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
      781 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
      841 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
      901 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
      961 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     1021 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     1081 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     1141 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     1201 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1261 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1321 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1381 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1441 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1501 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1561 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1621 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1681 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1741 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1801 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1861 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1921 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1981 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     2041 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     2101 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     2161 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa
     2221 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     2281 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     2341 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     2401 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     2461 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     2521 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
     2581 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
     2641 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
     2701 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
     2761 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
     2821 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
     2881 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
     2941 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
     3001 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
     3061 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
     3121 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
     3181 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
     3241 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
     3301 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
     3361 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
     3421 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
     3481 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
     3541 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
     3601 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     3661 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     3721 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
     3781 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
     3841 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
     3901 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
     3961 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
     4021 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
     4081 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
     4141 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
     4201 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     4261 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     4321 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4381 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     4441 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     4501 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     4561 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact
     4621 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     4681 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     4741 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     4801 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     4861 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     4921 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     4981 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     5041 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5101 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     5161 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5221 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     5281 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     5341 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     5401 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     5461 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     5521 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     5581 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     5641 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     5701 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
     5761 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     5821 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     5881 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     5941 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     6001 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     6061 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     6121 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     6181 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     6241 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     6301 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     6361 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     6421 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     6481 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     6541 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     6601 attagtgaac ggatctcgac ggtatcgcca aatggcagta ttcatccaca attttaaaag
     6661 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     6721 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     6781 cagggacagc agagatccag tttggatcga taagcttgat atcgaattcc tgcagccccg
     6841 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt
     6901 aggtttggca agctagctgc agtaacgcca ttttgcaagg catggaaaaa taccaaacca
     6961 agaatagaga agttcagatc aagggcgggt acatgaaaat agctaacgtt gggccaaaca
     7021 ggatatctgc ggtgagcagt ttcggccccg gcccggggcc aagaacagat ggtcaccgca
     7081 gtttcggccc cggcccgagg ccaagaacag atggtcccca gatatggccc aaccctcagc
     7141 agtttcttaa gacccatcag atgtttccag gctcccccaa ggacctgaaa tgaccctgcg
     7201 ccttatttga attaaccaat cagcctgctt ctcgcttctg ttcgcgcgct tctgcttccc
     7261 gagctctata aaagagctca caacccctca ctcggcgcgc cagtcctccg acagactgag
     7321 tcgcccgggg gggatctgga gctctcgaga attctcacgc gtgctagcta ccggtagcca
     7381 ccatggacta caaggatgac gacgataaga aagacaatac tgtgcctctg aagctgatcg
     7441 ctctcctggc taatggcgag ttccatagtg gcgaacagct gggagaaacc ctgggcatgt
     7501 ccagggccgc tatcaacaag cacattcaga ctctgcgcga ctggggcgtg gacgtgttca
     7561 ccgtgcccgg aaagggctac tctctgcccg agcctatccc gctgctgaac gctaaacaga
     7621 ttctgggaca gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc
     7681 agtacctgct ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc
     7741 agcaggcagg gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt
     7801 acctgagtat gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca
     7861 tcggaattgt catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat
     7921 ggcccaatga cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg
     7981 gaataacagg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc
     8041 gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc
     8101 tggacaggaa tactctggcc gctacgctga tccgagagct gcgggcagcc ctggaactgt
     8161 tcgagcagga aggcctggct ccatatctgc cacggtggga gaagctggat aacttcatca
     8221 atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg
     8281 ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag
     8341 aaatcagtct gcggtctgcc gaaaagtccg gactcagatc tcgagctcaa gcttcgaatt
     8401 ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg attaagatcc
     8461 ttgacttgcg gccgcaactc ccacctgcaa catgcgtgac tgactgaggc cgcgactcta
     8521 gagtcgacct gcaggcatgc aagcttgata tcaagcttat cgataatcaa cctctggatt
     8581 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     8641 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     8701 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     8761 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     8821 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     8881 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     8941 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     9001 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     9061 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     9121 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagggaatt
     9181 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     9241 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     9301 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     9361 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     9421 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     9481 tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc
     9541 gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt
     9601 acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa
     9661 ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc
     9721 acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg
     9781 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
     9841 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
     9901 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
     9961 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
    10021 aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc
    10081 gcatagttaa gccagcccc
//
