LOCUS       sequence-443634-       11391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Flag-tagged TurboID SQSTM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11391)
  AUTHORS   Ortiz ANR, Zhang L, Ash PEA, Basu A, Puri S, van der Spek SJF, Wang 
            Z, Dorrian L, Emili A, Wolozin B
  TITLE     Proximity labeling reveals dynamic changes in the SQSTM1 protein 
            network.
  JOURNAL   bioRxiv [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324.
  PUBMED    38168279
REFERENCE   2  (bases 1 to 11391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324."
COMMENT     Sequence Label: pHR-SFFV-FLAG-TurboID-SQSTM1
FEATURES             Location/Qualifiers
     source          1..11391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    190..315
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    812..1045
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1230..1274
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1559..1676
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1829..2236
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2277..2286
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2286..2309
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2310..3266
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    4764..5352
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4817..4837)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5235..5246)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5355..5371)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5356..5372)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5462..5695
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5720..5738)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5721..5738)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6028..6047)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6261..6280)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6380..6402
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6440..6458)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6526..6630
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6631..6699,6700..7491)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7662..8250
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8151..8170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8404..8421
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8491..8511)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8496..8825
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8676..8811
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9959..10024
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10154..10174
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10599..10733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10636..10655)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10690..10709
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10901..11391,1..143)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
       61 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      121 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
      181 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
      241 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
      301 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
      361 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
      421 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
      481 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
      541 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
      601 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc
      661 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
      721 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
      781 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
      841 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
      901 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
      961 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1021 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1081 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1141 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1201 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1261 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1321 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1381 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     1441 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     1501 ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc atccacaatt
     1561 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     1621 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     1681 tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc gaattcctgc
     1741 agccccgata aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc
     1801 cacctgtagg tttggcaagc tagctgcagt aacgccattt tgcaaggcat ggaaaaatac
     1861 caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc taacgttggg
     1921 ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag aacagatggt
     1981 caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat atggcccaac
     2041 cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga cctgaaatga
     2101 ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc gcgcgcttct
     2161 gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag tcctccgaca
     2221 gactgagtcg cccggggggg atctggagct ctcgagaatt ctcacgcgta ccggtagcca
     2281 ccatggacta caaggatgac gacgataaga aagacaatac tgtgcctctg aagctgatcg
     2341 ctctcctggc taatggcgag ttccatagtg gcgaacagct gggagaaacc ctgggcatgt
     2401 ccagggccgc tatcaacaag cacattcaga ctctgcgcga ctggggcgtg gacgtgttca
     2461 ccgtgcccgg aaagggctac tctctgcccg agcctatccc gctgctgaac gctaaacaga
     2521 ttctgggaca gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc
     2581 agtacctgct ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc
     2641 agcaggcagg gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt
     2701 acctgagtat gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca
     2761 tcggaattgt catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat
     2821 ggcccaatga cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg
     2881 gaataacagg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc
     2941 gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc
     3001 tggacaggaa tactctggcc gctacgctga tccgagagct gcgggcagcc ctggaactgt
     3061 tcgagcagga aggcctggct ccatatctgc cacggtggga gaagctggat aacttcatca
     3121 atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg
     3181 ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag
     3241 aaatcagtct gcggtctgcc gaaaagtccg gactcagatc tggtggcagt ggatctcgag
     3301 tcatggcgtc gctcaccgtg aaggcctacc ttctgggcaa ggaggacgcg gcgcgcgaga
     3361 ttcgccgctt cagcttctgc tgcagccccg agcctgaggc ggaagccgag gctgcggcgg
     3421 gtccgggacc ctgcgagcgg ctgctgagcc gggtggccgc cctgttcccc gcgctgcggc
     3481 ctggcggctt ccaggcgcac taccgcgatg aggacgggga cttggttgcc ttttccagtg
     3541 acgaggaatt gacaatggcc atgtcctacg tgaaggatga catcttccga atctacatta
     3601 aagagaaaaa agagtgccgg cgggaccacc gcccaccgtg tgctcaggag gcgccccgca
     3661 acatggtgca ccccaatgtg atctgcgatg gctgcaatgg gcctgtggta ggaacccgct
     3721 acaagtgcag cgtctgccca gactacgact tgtgtagcgt ctgcgaggga aagggcttgc
     3781 accgggggca caccaagctc gcattcccca gccccttcgg gcacctgtct gagggcttct
     3841 cgcacagccg ctggctccgg aaggtgaaac acggacactt cgggtggcca ggatgggaaa
     3901 tgggtccacc aggaaactgg agcccacgtc ctcctcgtgc aggggaggcc cgccctggcc
     3961 ccacggcaga atcagcttct ggtccatcgg aggatccgag tgtgaatttc ctgaagaacg
     4021 ttggggagag tgtggcagct gcccttagcc ctctgggcat tgaagttgat atcgatgtgg
     4081 agcacggagg gaaaagaagc cgcctgaccc ccgtctctcc agagagttcc agcacagagg
     4141 agaagagcag ctcacagcca agcagctgct gctctgatcc cagcaagccg ggtgggaatg
     4201 ttgagggcgc cacgcagtct ctggcggagc agatgagaaa gatcgccttg gagtccgagg
     4261 ggcgccctga ggaacagatg gagtcggata actgttcagg aggagatgat gactggaccc
     4321 atctgtcttc aaaagaagtg gacccgtcta caggtgaact ccagtcccta cagatgccag
     4381 aatccgaagg gccaagctct ctggacccct cccaggaggg acccacaggg ctgaaggaag
     4441 ctgccttgta cccacatctc ccgccagagg ctgacccgcg gctgattgag tccctctccc
     4501 agatgctgtc catgggcttc tctgatgaag gcggctggct caccaggctc ctgcagacca
     4561 agaactatga catcggagcg gctctggaca ccatccagta ttcaaagcat cccccgccgt
     4621 tgtaaggatc caccggatct agataactga ttaagatcct tgacttgcgg ccgcaactcc
     4681 cacctgcaac atgcgtgact gactgaggcc gcgactctag agtcgacctg caggcatgca
     4741 agcttgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     4801 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     4861 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     4921 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4981 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     5041 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     5101 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     5161 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     5221 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     5281 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     5341 ccgcctcccc gcatcgatac cgtcgacctc gagggaatta attcgagctc ggtaccttta
     5401 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     5461 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     5521 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5581 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5641 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatc
     5701 tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg atacataagg
     5761 ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat tgtgtagcat
     5821 ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg
     5881 ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac
     5941 caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg gccggcatca
     6001 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
     6061 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
     6121 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc
     6181 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
     6241 gtcgaatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     6301 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     6361 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     6421 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     6481 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6541 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6601 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6661 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6721 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6781 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6841 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6901 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6961 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     7021 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     7081 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     7141 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     7201 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     7261 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7321 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7381 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7441 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7501 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7561 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7621 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7681 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7741 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7801 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7861 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7921 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7981 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     8041 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8101 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8161 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8221 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8281 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8341 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8401 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     8461 gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     8521 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8581 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     8641 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     8701 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8761 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     8821 gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
     8881 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
     8941 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
     9001 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
     9061 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
     9121 attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
     9181 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
     9241 tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata
     9301 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct
     9361 tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata
     9421 cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct
     9481 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
     9541 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
     9601 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac
     9661 ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc
     9721 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc
     9781 caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg
     9841 attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc
     9901 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     9961 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
    10021 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
    10081 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
    10141 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
    10201 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
    10261 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
    10321 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
    10381 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
    10441 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
    10501 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
    10561 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
    10621 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    10681 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    10741 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
    10801 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    10861 gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc
    10921 caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
    10981 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
    11041 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
    11101 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
    11161 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
    11221 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
    11281 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
    11341 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc t
//
