LOCUS       sequence-443636-       11391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG-TurboID-mutKIR-SQSTM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11391)
  AUTHORS   Ortiz ANR, Zhang L, Ash PEA, Basu A, Puri S, van der Spek SJF, Wang 
            Z, Dorrian L, Emili A, Wolozin B
  TITLE     Proximity labeling reveals dynamic changes in the SQSTM1 protein 
            network.
  JOURNAL   bioRxiv [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324.
  PUBMED    38168279
REFERENCE   2  (bases 1 to 11391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324."
COMMENT     Sequence Label: pHR-SFFV-FLAG-TurboID-mutKIR-SQSTM1
FEATURES             Location/Qualifiers
     source          1..11391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(68..86)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(69..86)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(376..395)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(609..628)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     728..750
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(788..806)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        874..978
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(979..1047,1048..1839)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2010..2598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2499..2518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2752..2769
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2839..2859)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2844..3173
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3024..3159
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4307..4372
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4502..4522
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4947..5081
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4984..5003)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5038..5057
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5249..5882
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5929..6054
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6551..6784
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6969..7013
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7298..7415
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7568..7975
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      8016..8025
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8025..8048
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             8049..9005
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    10503..11091
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10556..10576)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10974..10985)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11094..11110)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11095..11111)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             join(11201..11391,1..43)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gaattaattc
       61 cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat
      121 tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga
      181 agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct
      241 tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg
      301 tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
      361 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
      421 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
      481 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca
      541 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc
      601 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
      661 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
      721 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
      781 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      841 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      901 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      961 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     1021 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     1081 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     1141 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     1201 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     1261 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     1321 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     1381 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     1441 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     1501 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     1561 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     1621 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     1681 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     1741 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     1801 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     1861 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1921 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1981 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2041 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2101 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     2161 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2221 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2281 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2341 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2401 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2461 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2521 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2581 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2641 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2701 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2761 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     2821 attcattaat gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     2881 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     2941 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3001 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     3061 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     3121 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
     3181 gacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga
     3241 ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc
     3301 tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct
     3361 gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca
     3421 cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc
     3481 cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata
     3541 ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg
     3601 cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg
     3661 cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg
     3721 ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac
     3781 agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac
     3841 gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta
     3901 agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc
     3961 cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg
     4021 cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt
     4081 ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt
     4141 gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt
     4201 ggcggcaact ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg
     4261 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
     4321 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
     4381 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
     4441 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
     4501 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
     4561 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
     4621 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
     4681 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
     4741 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
     4801 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
     4861 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     4921 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     4981 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5041 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
     5101 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
     5161 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     5221 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
     5281 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     5341 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     5401 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     5461 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     5521 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     5581 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     5641 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     5701 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5761 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5821 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5881 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     5941 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     6001 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     6061 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     6121 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     6181 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     6241 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     6301 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     6361 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag
     6421 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     6481 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     6541 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     6601 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     6661 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     6721 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     6781 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     6841 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     6901 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     6961 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     7021 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     7081 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     7141 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     7201 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     7261 ctcgacggta tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     7321 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     7381 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     7441 atccagtttg gatcgataag cttgatatcg aattcctgca gccccgataa aataaaagat
     7501 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
     7561 agctgcagta acgccatttt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt
     7621 cagatcaagg gcgggtacat gaaaatagct aacgttgggc caaacaggat atctgcggtg
     7681 agcagtttcg gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc
     7741 ccgaggccaa gaacagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc
     7801 catcagatgt ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta
     7861 accaatcagc ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag
     7921 agctcacaac ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccggggggga
     7981 tctggagctc tcgagaattc tcacgcgtac cggtagccac catggactac aaggatgacg
     8041 acgataagaa agacaatact gtgcctctga agctgatcgc tctcctggct aatggcgagt
     8101 tccatagtgg cgaacagctg ggagaaaccc tgggcatgtc cagggccgct atcaacaagc
     8161 acattcagac tctgcgcgac tggggcgtgg acgtgttcac cgtgcccgga aagggctact
     8221 ctctgcccga gcctatcccg ctgctgaacg ctaaacagat tctgggacag ctggacggcg
     8281 ggagcgtggc agtcctgcct gtggtcgact ccaccaatca gtacctgctg gatcgaatcg
     8341 gcgagctgaa gagtggggat gcttgcattg cagaatatca gcaggcaggg agaggaagca
     8401 gagggaggaa atggttctct ccttttggag ctaacctgta cctgagtatg ttttggcgcc
     8461 tgaagcgggg accagcagca atcggcctgg gcccggtcat cggaattgtc atggcagaag
     8521 cgctgcgaaa gctgggagca gacaaggtgc gagtcaaatg gcccaatgac ctgtatctgc
     8581 aggatagaaa gctggcaggc atcctggtgg agctggccgg aataacaggc gatgctgcac
     8641 agatcgtcat tggcgccggg attaacgtgg ctatgaggcg cgtggaggaa agcgtggtca
     8701 atcagggctg gatcacactg caggaagcag ggattaacct ggacaggaat actctggccg
     8761 ctacgctgat ccgagagctg cgggcagccc tggaactgtt cgagcaggaa ggcctggctc
     8821 catatctgcc acggtgggag aagctggata acttcatcaa tagacccgtg aagctgatca
     8881 ttggggacaa agagattttc gggattagcc gggggattga taaacaggga gccctgctgc
     8941 tggaacagga cggagttatc aaaccctgga tgggcggaga aatcagtctg cggtctgccg
     9001 aaaagtccgg actcagatct ggtggcagtg gatctcgagt catggcgtcg ctcaccgtga
     9061 aggcctacct tctgggcaag gaggacgcgg cgcgcgagat tcgccgcttc agcttctgct
     9121 gcagccccga gcctgaggcg gaagccgagg ctgcggcggg tccgggaccc tgcgagcggc
     9181 tgctgagccg ggtggccgcc ctgttccccg cgctgcggcc tggcggcttc caggcgcact
     9241 accgcgatga ggacggggac ttggttgcct tttccagtga cgaggaattg acaatggcca
     9301 tgtcctacgt gaaggatgac atcttccgaa tctacattaa agagaaaaaa gagtgccggc
     9361 gggaccaccg cccaccgtgt gctcaggagg cgccccgcaa catggtgcac cccaatgtga
     9421 tctgcgatgg ctgcaatggg cctgtggtag gaacccgcta caagtgcagc gtctgcccag
     9481 actacgactt gtgtagcgtc tgcgagggaa agggcttgca ccgggggcac accaagctcg
     9541 cattccccag ccccttcggg cacctgtctg agggcttctc gcacagccgc tggctccgga
     9601 aggtgaaaca cggacacttc gggtggccag gatgggaaat gggtccacca ggaaactgga
     9661 gcccacgtcc tcctcgtgca ggggaggccc gccctggccc cacggcagaa tcagcttctg
     9721 gtccatcgga ggatccgagt gtgaatttcc tgaagaacgt tggggagagt gtggcagctg
     9781 cccttagccc tctgggcatt gaagttgata tcgatgtgga gcacggaggg aaaagaagcc
     9841 gcctgacccc cgtctctcca gagagttcca gcacagagga gaagagcagc tcacagccaa
     9901 gcagctgctg ctctgatccc agcaagccgg gtgggaatgt tgagggcgcc acgcagtctc
     9961 tggcggagca gatgagaaag atcgccttgg agtccgaggg gcgccctgag gaacagatgg
    10021 agtcggataa ctgttcagga ggagatgatg actggaccca tctgtcttca aaagaagtgg
    10081 ccccgtctgc tgctgcgctc cagtccctac agatgccaga atccgaaggg ccaagctctc
    10141 tggacccctc ccaggaggga cccacagggc tgaaggaagc tgccttgtac ccacatctcc
    10201 cgccagaggc tgacccgcgg ctgattgagt ccctctccca gatgctgtcc atgggcttct
    10261 ctgatgaagg cggctggctc accaggctcc tgcagaccaa gaactatgac atcggagcgg
    10321 ctctggacac catccagtat tcaaagcatc ccccgccgtt gtaaggatcc accggatcta
    10381 gataactgat taagatcctt gacttgcggc cgcaactccc acctgcaaca tgcgtgactg
    10441 actgaggccg cgactctaga gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg
    10501 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
    10561 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
    10621 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
    10681 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
    10741 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
    10801 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
    10861 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
    10921 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
    10981 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
    11041 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
    11101 gtcgacctcg agggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca
    11161 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
    11221 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
    11281 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    11341 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta g
//
