LOCUS       sequence-443637-       10394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Flag-tagged SQSTM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10394)
  AUTHORS   Ortiz ANR, Zhang L, Ash PEA, Basu A, Puri S, van der Spek SJF, Wang 
            Z, Dorrian L, Emili A, Wolozin B
  TITLE     Proximity labeling reveals dynamic changes in the SQSTM1 protein 
            network.
  JOURNAL   bioRxiv [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324.
  PUBMED    38168279
REFERENCE   2  (bases 1 to 10394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324."
COMMENT     Sequence Label: pHR-SFFV-FLAG-SQSTM1
FEATURES             Location/Qualifiers
     source          1..10394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..641
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    688..813
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1310..1543
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1728..1772
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2057..2174
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2327..2734
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2771..2794
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4265..4853
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4318..4338)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4736..4747)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4856..4872)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4857..4873)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4963..5196
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5221..5239)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5222..5239)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5529..5548)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5762..5781)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5881..5903
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5941..5959)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6027..6131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6132..6200,6201..6992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6350..6369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7163..7751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7652..7671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7905..7922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7992..8012)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7997..8326
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8177..8312
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9460..9525
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9655..9675
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10100..10234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10137..10156)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10191..10210
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
       61 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
      121 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
      181 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
      241 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
      301 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
      361 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
      421 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
      481 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      541 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      601 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
      661 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
      721 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
      781 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
      841 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
      901 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
      961 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     1021 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     1081 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     1141 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1201 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1261 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1321 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1381 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1441 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1501 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1561 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1621 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1681 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1741 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1801 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1861 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1921 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     1981 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgccaaatgg
     2041 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     2101 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     2161 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg atcgataagc
     2221 ttgatatcga attcctgcag ccccgataaa ataaaagatt ttatttagtc tccagaaaaa
     2281 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gctgcagtaa cgccattttg
     2341 caaggcatgg aaaaatacca aaccaagaat agagaagttc agatcaaggg cgggtacatg
     2401 aaaatagcta acgttgggcc aaacaggata tctgcggtga gcagtttcgg ccccggcccg
     2461 gggccaagaa cagatggtca ccgcagtttc ggccccggcc cgaggccaag aacagatggt
     2521 ccccagatat ggcccaaccc tcagcagttt cttaagaccc atcagatgtt tccaggctcc
     2581 cccaaggacc tgaaatgacc ctgcgcctta tttgaattaa ccaatcagcc tgcttctcgc
     2641 ttctgttcgc gcgcttctgc ttcccgagct ctataaaaga gctcacaacc cctcactcgg
     2701 cgcgccagtc ctccgacaga ctgagtcgcc cgggggggat ctggagctct cgagaattct
     2761 cacgcgtatg gattacaagg atgacgacga taagggagct ggtatggcgt cgctcaccgt
     2821 gaaggcctac cttctgggca aggaggacgc ggcgcgcgag attcgccgct tcagcttctg
     2881 ctgcagcccc gagcctgagg cggaagccga ggctgcggcg ggtccgggac cctgcgagcg
     2941 gctgctgagc cgggtggccg ccctgttccc cgcgctgcgg cctggcggct tccaggcgca
     3001 ctaccgcgat gaggacgggg acttggttgc cttttccagt gacgaggaat tgacaatggc
     3061 catgtcctac gtgaaggatg acatcttccg aatctacatt aaagagaaaa aagagtgccg
     3121 gcgggaccac cgcccaccgt gtgctcagga ggcgccccgc aacatggtgc accccaatgt
     3181 gatctgcgat ggctgcaatg ggcctgtggt aggaacccgc tacaagtgca gcgtctgccc
     3241 agactacgac ttgtgtagcg tctgcgaggg aaagggcttg caccgggggc acaccaagct
     3301 cgcattcccc agccccttcg ggcacctgtc tgagggcttc tcgcacagcc gctggctccg
     3361 gaaggtgaaa cacggacact tcgggtggcc aggatgggaa atgggtccac caggaaactg
     3421 gagcccacgt cctcctcgtg caggggaggc ccgccctggc cccacggcag aatcagcttc
     3481 tggtccatcg gaggatccga gtgtgaattt cctgaagaac gttggggaga gtgtggcagc
     3541 tgcccttagc cctctgggca ttgaagttga tatcgatgtg gagcacggag ggaaaagaag
     3601 ccgcctgacc cccgtctctc cagagagttc cagcacagag gagaagagca gctcacagcc
     3661 aagcagctgc tgctctgatc ccagcaagcc gggtgggaat gttgagggcg ccacgcagtc
     3721 tctggcggag cagatgagaa agatcgcctt ggagtccgag gggcgccctg aggaacagat
     3781 ggagtcggat aactgttcag gaggagatga tgactggacc catctgtctt caaaagaagt
     3841 ggacccgtct acaggtgaac tccagtccct acagatgcca gaatccgaag ggccaagctc
     3901 tctggacccc tcccaggagg gacccacagg gctgaaggaa gctgccttgt acccacatct
     3961 cccgccagag gctgacccgc ggctgattga gtccctctcc cagatgctgt ccatgggctt
     4021 ctctgatgaa ggcggctggc tcaccaggct cctgcagacc aagaactatg acatcggagc
     4081 ggctctggac accatccagt attcaaagca tcccccgccg ttgtaaggat ccaccggatc
     4141 tagataactg attaagatcc ttgacttgcg gccgcaactc ccacctgcaa catgcgtgac
     4201 tgactgaggc cgcgactcta gagtcgacct gcaggcatgc aagcttgata tcaagcttat
     4261 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     4321 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     4381 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4441 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4501 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4561 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4621 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     4681 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4741 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4801 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     4861 ccgtcgacct cgagggaatt aattcgagct cggtaccttt aagaccaatg acttacaagg
     4921 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     4981 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     5041 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5101 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5161 tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat
     5221 tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc
     5281 gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac
     5341 cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt
     5401 ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc
     5461 tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
     5521 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
     5581 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
     5641 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
     5701 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca
     5761 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     5821 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     5881 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     5941 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     6001 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6061 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     7321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7981 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     8041 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     8101 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     8161 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8221 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8281 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
     8341 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
     8401 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
     8461 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
     8521 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
     8581 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
     8641 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
     8701 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
     8761 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
     8821 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
     8881 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
     8941 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
     9001 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
     9061 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
     9121 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
     9181 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
     9241 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
     9301 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
     9361 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
     9421 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
     9481 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
     9541 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
     9601 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
     9661 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
     9721 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
     9781 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
     9841 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
     9901 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
     9961 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
    10021 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    10081 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    10141 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    10201 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
    10261 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
    10321 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
    10381 atgtactaca aact
//
