LOCUS       sequence-443638-       10015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Alias: G-alpha-i bONE-GO W139A. Lentiviral vector expressing 
            GINIP-Nluc W139A after a low efficiency IRES downstream of YFP-CAAX 
            placed after the promotor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Luebbers A, Janicot R, Zhao J, Philibert CE, Garcia-Marcos M
  TITLE     A sensitive biosensor of endogenous Galpha(i) activity enables the 
            accurate characterization of endogenous GPCR agonist responses.
  JOURNAL   Sci Signal. 2025 Mar 25;18(879):eadp6457. doi: 
            10.1126/scisignal.adp6457. Epub 2025 Mar 25.
  PUBMED    40132053
REFERENCE   2  (bases 1 to 10015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2025-03-25"; volume: "18(879):eadp6457. doi"; 
            pages: " 10.1126/scisignal.adp6457. Epub 2025 Mar 25"
COMMENT     Sequence Label: pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc W139A
FEATURES             Location/Qualifiers
     source          1..10015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    138..253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(516..560)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    745..978
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1475..1600
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1647..2280
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2448..2582
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2472..2491)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2526..2545
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2630..2734
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2735..2803,2804..3595)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2953..2972)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3766..4354
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4255..4274
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4508..4525
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4642..4663
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4678..4683,4684..4701,4702..4708)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4716..4732
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4721..4743
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4740..4756
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4740..4756
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             4884..5517
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5724..6312
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5830..5841
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6239..6259
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6326..6841)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    join(8064..8072,8073..8075,8076..8650)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8237..8256)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     8466..8483
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(8739..9449,9450..9452,9453..9455))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8781..8802)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9130..9149
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9389..9410
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9567..9591)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(9588..9791)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9617..9637)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        join(9792..10015,1..80)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
       61 ttaccgtaag ttatgtaacg cggaactccc aagcttatcg ataaactgga tctctgctgt
      121 ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt
      181 gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc
      241 cttttctttt aaaggcgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg
      301 tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg
      361 aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt
      421 acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata
      481 taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc
      541 aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt
      601 aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc
      661 tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt
      721 tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc
      781 aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc
      841 gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg
      901 gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac
      961 ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt
     1021 gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt atataattca
     1081 cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg ccggccgctt
     1141 gctgtgcggt ggtcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg
     1201 tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg
     1261 atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac
     1321 agccttctga tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg
     1381 cccatactat atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt
     1441 cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct
     1501 tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg
     1561 ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg
     1621 ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct
     1681 gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca
     1741 aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc
     1801 tcagatctgg tctaaccaga gagacccagt acaggcaaaa agcagctgct tatatgcagg
     1861 atctgagggc tcgccactcc ccagtcccgc ccaggccacg cctccctgga aagtccccag
     1921 cggaaagtcc cttgtagcaa gctcgatatc agcagttctt gaagtactcc ggatgcagct
     1981 ctcgggccac gtgatgaaat gctaggcggc tgtcaaacct ccactctaac acttctctct
     2041 ccgggtcatc catcccatgc aggctcacag ggtgtaacaa gctggtgttc tctcctttat
     2101 tggcctcttc taccttatct ggctcaactg gtactagctt gtagcaccat ccaaaggtca
     2161 gtggatatct gacccctggc cctggtgtgt agttctgcta atcagggaag tagccttgtg
     2221 tgtggtagat ccacagatca aggatatctt gtcttctttg ggagtgaatt agcccttcca
     2281 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaatga aaataattca
     2341 gaggaatcac aggtttagag taaatgaaac cacaggtaat tggcagtggt aatagggtat
     2401 ggggtgggaa gtttgggatg attttggtta gcttgagtta tccagttgat ccagacatga
     2461 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     2521 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     2581 ttgatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     2641 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     2701 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     2761 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     2821 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     2881 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     2941 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     3001 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     3061 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     3121 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     3181 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     3241 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     3301 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     3361 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     3421 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     3481 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     3541 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     3601 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     3661 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3721 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3781 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3841 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3901 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3961 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4021 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4081 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4141 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4201 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4261 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4321 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4381 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4441 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4501 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     4561 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     4621 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     4681 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     4741 aggaaacagc tatgaccatg attacgccaa gctctagcta gaggtcgacg gtaaacgcta
     4801 gcaatgtcaa ggcctctcac tctctgatat tcatttcttt gcaagttata aatactgaat
     4861 aataagatga catgaactac tactgctaga gattttccac actgactaaa agggtctgag
     4921 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     4981 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     5041 gatctggtct aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc
     5101 tgagggctcg ccactcccca gtcccgccca ggccacgcct ccctggaaag tccccagcgg
     5161 aaagtccctt gtagcaagct cgatatcagc agttcttgaa gtactccgga tgcagctctc
     5221 gggccacgtg atgaaatgct aggcggctgt caaacctcca ctctaacact tctctctccg
     5281 ggtcatccat cccatgcagg ctcacagggt gtaacaagct ggtgttctct cctttattgg
     5341 cctcttctac cttatctggc tcaactggta ctagcttgta gcaccatcca aaggtcagtg
     5401 gatatctgac ccctggccct ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt
     5461 ggtagatcca cagatcaagg atatcttgtc ttcgttggga gtgaattagc ccttccagtc
     5521 ccctcttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta
     5581 aaggtacctg aggtgtgact ggaaaaccca cctcctcctc ctcttgtgct tctagccagg
     5641 cacaatcagc attggtagct gctgtattgc tacttgtgat tgctccatgt ttttctaggt
     5701 ctcgactgca gaattaattc caggcgggga ggcggcccaa agggagatcc gactcgtctg
     5761 agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag
     5821 gtccgctgga ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt
     5881 ggcaacacag gcgagcagcc atggaaagga cgtcagcttc cccgacaaca ccacggaatt
     5941 gtcagtgccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc
     6001 cgccgtggca atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc
     6061 aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct
     6121 gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa
     6181 aatgaaagcc atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca
     6241 tagcgtaaaa ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc
     6301 cagaggttga ttgttccaga cgcgtttacg ccagaatgcg ttcgcacagc cgccagccgg
     6361 tcactccgtt gatggttact cggaacagca gggagccgtc ggggttgatc aggcgctcgt
     6421 cgataatttt gttgccgttc cacagggtcc ctgttacagt gatctttttg ccgtcgaaca
     6481 cggcgatgcc ttcatacggc cgtccgaaat agtcgatcat gttcggcgta accccgtcga
     6541 ttaccagtgt gccatagtgc aggatcacct taaagtgatg atcatccaca gggtacacca
     6601 ccttaaaaat tttttcgatc tggcccattt ggtcgccgct cagaccttca tacgggatga
     6661 tgacatggat gtcgatcttc agcccatttt caccgctcag gacaatcctt tggatcggag
     6721 ttacggacac cccgagattc tgaaacaaac tggacacacc tccctgttca aggacttggt
     6781 ccaggttgta gccggctgtc tgtcgccagt ccccaacgaa atcttcgagt gtgaagacca
     6841 tagaaccacc acctcctggg ggcttcaggt gaatggctgc gctgttgggg cgagcagcca
     6901 ggatgtagag gacattctct tgcaggaagg taggccagtc cacaaatctg gactcctgct
     6961 ctgtgggcag cagggtcttg tcctgactct tgctgctgct gctggctggg ctgctatctg
     7021 cgatgggacc cacatggctg atgctgacac tgcaggaagg agcctctggg aaccgtttgc
     7081 aaaaccaaga gtgctgggcc cggcggcact ctgcctcact gacggtgctc ccactgcccc
     7141 gagccaggaa ggcggctcgg gctcgctccc gctccttcac tgtcagaagc ctcaacagag
     7201 agttctgggg acgcaactgc tgcagaagca ggagggactc atgggacaag aagtcaggcc
     7261 actctatgtg gcctcgatgt tcagggtcca gggcagcaaa ctggcgggcc gcctgctctt
     7321 cttgctcctc actcagggcc ctgtcacggt ccccccgctt ggctgcttgg tggcggtaac
     7381 gcagaaagtc ctccagtgtc agggagcaat cagggatgac tttacaccgc tgaaaggtct
     7441 ccgtgaggct atacatttcc tcctcagtaa gcagcaagtt gatgttgtca cagtagtggc
     7501 agctccagcc tgtttctgtg tgggccatct ccgtcacctc cgctgcactg tctccttgga
     7561 tgtagcccat gcggcgcagg cagccatcat ggaaaaccct ggtgcagacc ctgcacggga
     7621 agaggctctc agctgtcgcg acctcacaaa catcacacat ctcatcgttg acaacaggct
     7681 ctctgggctc caggcagatt tctggaggtt tgtcatcatc cagcttccgg gtggggcgga
     7741 tgaacgcagg gggtgtgaat cgacttgtcc tgtcaaactc ctcaggctcc acgccgcgcc
     7801 catctcggag ccgctcccag gctgcgcggc cggcactact ctcttcctgg accaccgagg
     7861 ttcctgcttc tgcttccttc tcctcctgta cctcctggac ggagccctct acagtgccac
     7921 ggcgggaccc tggcagctca cctgtgcgtc ggatggaagg cctgtcccgc agcccatcct
     7981 tgaaagcaga cacagccaga ctcaccttct gcacctgctc cactgtctgc cgcttggaca
     8041 tcaacacccc catctttggt acgggttgtg gcaagcttat catcgtgttt ttcaaaggaa
     8101 aaccacgtcc ccgtggttcg gggggcctag acgttttttt aacctcgact aaacacatgt
     8161 aaagcatgtg caccgaggcc ccagatcaga tcccatacaa tggggtacct tctgggcatc
     8221 cttcagcccc ttgttgaata cgcttgagga gagccatttg actctttcca caactatcca
     8281 actcacaacg tggcactggg gttgtgccgc ctttgcaggt gtatcttata cacgtggctt
     8341 ttggccgcag aggcacctgt cgccaggtgg ggggttccgc tgcctgcaaa gggtcgctac
     8401 agacgttgtt tgtcttcaag aagcttccag aggaactgct tccttcacga cattcaacag
     8461 accttgcatt cctttggcga gaggggaaag acccctagga atgctcgtca agaagacagg
     8521 gccaggtttc cgggccctca cattgccaaa agacggcaat atggtggaaa ataacatata
     8581 gacaaacgca caccggcctt attccaagcg gcttcggcca gtaacgttag ggggggggga
     8641 gggagagggg cggcggccgc ttacataatt acacactttg tctttgactt ctttttcttc
     8701 tttttaccat ctttgctcat cttttcttta tgttttcgct tgtacagctc gtccatgccg
     8761 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
     8821 gggtctttgc tcagcttgga ctggtagctc aggtagtggt tgtcgggcag cagcacgggg
     8881 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgcc gccgtcctcg
     8941 atgttgtggc ggatcttgaa gttggccttg atgccgttct tctgcttgtc ggcggtgata
     9001 tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
     9061 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
     9121 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
     9181 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg
     9241 aagcactgca ggccgtagcc gagggtggtc acgagggtgg gccagggcac gggcagcttg
     9301 ccggtggtgc agatgagctt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
     9361 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
     9421 accccggtga acagctcctc gcccttgctc accatggatc cgcggccgct ctagaactag
     9481 tctcgaggaa ttcaccggaa atagatcctc tagtagagtc ggtgtcttct atggaggtca
     9541 aaacagcgtg gatggcgtct ccaggcgatc tgacggttca ctaaacgagc tctgcttata
     9601 tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga
     9661 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg
     9721 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa
     9781 aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
     9841 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
     9901 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
     9961 aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaa
//
