LOCUS       sequence-443639-       10009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Alias: G-alpha-i bONE-GO. Lentiviral vector expressing GINIP-Nluc 
            after a low efficiency IRES downstream of YFP-CAAX placed after the 
            promotor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10009)
  AUTHORS   Luebbers A, Janicot R, Zhao J, Philibert CE, Garcia-Marcos M
  TITLE     A sensitive biosensor of endogenous Galpha(i) activity enables the 
            accurate characterization of endogenous GPCR agonist responses.
  JOURNAL   Sci Signal. 2025 Mar 25;18(879):eadp6457. doi: 
            10.1126/scisignal.adp6457. Epub 2025 Mar 25.
  PUBMED    40132053
REFERENCE   2  (bases 1 to 10009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2025-03-25"; volume: "18(879):eadp6457. doi"; 
            pages: " 10.1126/scisignal.adp6457. Epub 2025 Mar 25"
COMMENT     Sequence Label: pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc
FEATURES             Location/Qualifiers
     source          1..10009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    135..250
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(513..557)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    742..975
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1472..1597
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1644..2277
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2445..2579
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2469..2488)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2523..2542
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2627..2731
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2732..2800,2801..3592)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2950..2969)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3763..4351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4252..4271
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4505..4522
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4639..4660
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4675..4680,4681..4698,4699..4705)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4713..4729
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4718..4740
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4737..4753
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4737..4753
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             4881..5514
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5721..6309
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5827..5838
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6236..6256
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6323..6838)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(8232..8251)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     8461..8478
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(8730..9440,9441..9443,9444..9446))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8772..8793)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9121..9140
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9380..9401
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9558..9582)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(9579..9782)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9608..9628)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        join(9783..10009,1..77)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
       61 ccgtaagtta tgtaacgcgg aactcccaag cttatcgata aactggatct ctgctgtccc
      121 tgtaataaac ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta
      181 tgtctgttgc tattatgtct actattcttt cccctgcact gtacccccca atcccccctt
      241 ttcttttaaa ggcgataccg tcgagatccg ttcactaatc gaatggatct gtctctgtct
      301 ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg
      361 tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca
      421 gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac
      481 cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat
      541 aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag
      601 cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt
      661 tccagagatt tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt
      721 ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg
      781 attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc
      841 tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc
      901 tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca
      961 aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc
     1021 ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata taattcactt
     1081 ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg gccgcttgct
     1141 gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt
     1201 cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc
     1261 taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc
     1321 cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc
     1381 atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc
     1441 atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct
     1501 agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg
     1561 tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt
     1621 tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag
     1681 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     1741 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     1801 gatctggtct aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc
     1861 tgagggctcg ccactcccca gtcccgccca ggccacgcct ccctggaaag tccccagcgg
     1921 aaagtccctt gtagcaagct cgatatcagc agttcttgaa gtactccgga tgcagctctc
     1981 gggccacgtg atgaaatgct aggcggctgt caaacctcca ctctaacact tctctctccg
     2041 ggtcatccat cccatgcagg ctcacagggt gtaacaagct ggtgttctct cctttattgg
     2101 cctcttctac cttatctggc tcaactggta ctagcttgta gcaccatcca aaggtcagtg
     2161 gatatctgac ccctggccct ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt
     2221 ggtagatcca cagatcaagg atatcttgtc ttctttggga gtgaattagc ccttccaact
     2281 actaagtttg tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag
     2341 gaatcacagg tttagagtaa atgaaaccac aggtaattgg cagtggtaat agggtatggg
     2401 gtgggaagtt tgggatgatt ttggttagct tgagttatcc agttgatcca gacatgataa
     2461 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     2521 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttg
     2581 atctcccgat ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     2641 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2701 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     2761 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     2821 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     2881 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     2941 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     3001 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3061 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3121 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3181 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3241 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     3301 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     3361 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     3421 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     3481 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     3541 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     3601 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     3661 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     3721 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3781 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3841 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3901 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3961 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4021 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4081 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4141 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4201 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4261 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4321 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4381 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4441 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4501 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     4561 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     4621 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     4681 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     4741 aaacagctat gaccatgatt acgccaagct ctagctagag gtcgacggta aacgctagca
     4801 atgtcaaggc ctctcactct ctgatattca tttctttgca agttataaat actgaataat
     4861 aagatgacat gaactactac tgctagagat tttccacact gactaaaagg gtctgaggga
     4921 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
     4981 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
     5041 ctggtctaac cagagagacc cagtacaggc aaaaagcagc tgcttatatg caggatctga
     5101 gggctcgcca ctccccagtc ccgcccaggc cacgcctccc tggaaagtcc ccagcggaaa
     5161 gtcccttgta gcaagctcga tatcagcagt tcttgaagta ctccggatgc agctctcggg
     5221 ccacgtgatg aaatgctagg cggctgtcaa acctccactc taacacttct ctctccgggt
     5281 catccatccc atgcaggctc acagggtgta acaagctggt gttctctcct ttattggcct
     5341 cttctacctt atctggctca actggtacta gcttgtagca ccatccaaag gtcagtggat
     5401 atctgacccc tggccctggt gtgtagttct gctaatcagg gaagtagcct tgtgtgtggt
     5461 agatccacag atcaaggata tcttgtcttc gttgggagtg aattagccct tccagtcccc
     5521 tcttttcttt taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag
     5581 gtacctgagg tgtgactgga aaacccacct cctcctcctc ttgtgcttct agccaggcac
     5641 aatcagcatt ggtagctgct gtattgctac ttgtgattgc tccatgtttt tctaggtctc
     5701 gactgcagaa ttaattccag gcggggaggc ggcccaaagg gagatccgac tcgtctgagg
     5761 gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc
     5821 cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc
     5881 aacacaggcg agcagccatg gaaaggacgt cagcttcccc gacaacacca cggaattgtc
     5941 agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc
     6001 cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat
     6061 gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac
     6121 aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat
     6181 gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag
     6241 cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag
     6301 aggttgattg ttccagacgc gtttacgcca gaatgcgttc gcacagccgc cagccggtca
     6361 ctccgttgat ggttactcgg aacagcaggg agccgtcggg gttgatcagg cgctcgtcga
     6421 taattttgtt gccgttccac agggtccctg ttacagtgat ctttttgccg tcgaacacgg
     6481 cgatgccttc atacggccgt ccgaaatagt cgatcatgtt cggcgtaacc ccgtcgatta
     6541 ccagtgtgcc atagtgcagg atcaccttaa agtgatgatc atccacaggg tacaccacct
     6601 taaaaatttt ttcgatctgg cccatttggt cgccgctcag accttcatac gggatgatga
     6661 catggatgtc gatcttcagc ccattttcac cgctcaggac aatcctttgg atcggagtta
     6721 cggacacccc gagattctga aacaaactgg acacacctcc ctgttcaagg acttggtcca
     6781 ggttgtagcc ggctgtctgt cgccagtccc caacgaaatc ttcgagtgtg aagaccatag
     6841 aaccaccacc tcctgggggc ttcaggtgaa tggctgcgct gttggggcga gcagccagga
     6901 tgtagaggac attctcttgc aggaaggtag gccagtccac aaatctggac tcctgctctg
     6961 tgggcagcag ggtcttgtcc tgactcttgc tgctgctgct ggctgggctg ctatctgcga
     7021 tgggacccac atggctgatg ctgacactgc aggaaggagc ctctgggaac cgtttgcaaa
     7081 accaagagtg ctgggcccgg cggcactctg cctcactgac ggtgctccca ctgccccgag
     7141 ccaggaaggc ggctcgggct cgctcccgct ccttcactgt cagaagcctc aacagagagt
     7201 tctggggacg caactgctgc agaagcagga gggactcatg ggacaagaag tcaggccact
     7261 ctatgtggcc tcgatgttca gggtccaggg cagcaaactg gcgggccgcc tgctcttctt
     7321 gctcctcact cagggccctg tcacggtccc cccgcttggc tgcttggtgg cggtaacgca
     7381 gaaagtcctc cagtgtcagg gagcaatcag ggatgacttt acaccgctga aaggtctccg
     7441 tgaggctata catttcctcc tcagtaagca gcaagttgat gttgtcacag tagtggcagc
     7501 tccagcctgt ttctgtgtgg gccatctccg tcacctccgc tgcactgtct ccttggatgt
     7561 agcccatgcg gcgcaggcag ccatcatgga aaaccctggt gcagaccctg cacgggaaga
     7621 ggctctcagc tgtccagacc tcacaaacat cacacatctc atcgttgaca acaggctctc
     7681 tgggctccag gcagatttct ggaggtttgt catcatccag cttccgggtg gggcggatga
     7741 acgcaggggg tgtgaatcga cttgtcctgt caaactcctc aggctccacg ccgcgcccat
     7801 ctcggagccg ctcccaggct gcgcggccgg cactactctc ttcctggacc accgaggttc
     7861 ctgcttctgc ttccttctcc tcctgtacct cctggacgga gccctctaca gtgccacggc
     7921 gggaccctgg cagctcacct gtgcgtcgga tggaaggcct gtcccgcagc ccatccttga
     7981 aagcagacac agccagactc accttctgca cctgctccac tgtctgccgc ttggacatca
     8041 acacccccat ctttggtacg ggttgtggca agctatcatc gttttttcaa aggaaaacca
     8101 cgtccccgtg gttcgggggg cctagacgtt tttttaacct cgactaaaca catgtaaagc
     8161 atgtgcaccg aggccccaga tcagatccca tacaatgggg taccttctgg gcatccttca
     8221 gccccttgtt gaatacgctt gaggagagcc atttgactct ttccacaact atccaactca
     8281 caacgtggca ctggggttgt gccgcctttg caggtgtatc ttatacacgt ggcttttggc
     8341 cgcagaggca cctgtcgcca ggtggggggt tccgctgcct gcaaagggtc gctacagacg
     8401 ttgtttgtct tcaagaagct tccagaggaa ctgcttcctt cacgacattc aacagacctt
     8461 gcattccttt ggcgagaggg gaaagacccc taggaatgct cgtcaagaag acagggccag
     8521 gtttccgggc cctcacattg ccaaaagacg gcaatatggt ggaaaataac atatagacaa
     8581 acgcacaccg gccttattcc aagcggcttc ggccagtaac gttagggggg ggggagggag
     8641 gcggcggccg cttacataat tacacacttt gtctttgact tctttttctt ctttttacca
     8701 tctttgctca tcttttcttt atgttttcgc ttgtacagct cgtccatgcc gagagtgatc
     8761 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
     8821 ctcagcttgg actggtagct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
     8881 atgggggtgt tctgctggta gtggtcggcg agctgcacgc cgccgtcctc gatgttgtgg
     8941 cggatcttga agttggcctt gatgccgttc ttctgcttgt cggcggtgat atagacgttg
     9001 tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
     9061 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
     9121 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc
     9181 atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc gaagcactgc
     9241 aggccgtagc cgagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg
     9301 cagatgagct tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg
     9361 ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg
     9421 aacagctcct cgcccttgct caccatggat ccgcggccgc tctagaacta gtctcgagga
     9481 attcaccgga aatagatcct ctagtagagt cggtgtcttc tatggaggtc aaaacagcgt
     9541 ggatggcgtc tccaggcgat ctgacggttc actaaacgag ctctgcttat atagacctcc
     9601 caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga
     9661 aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt
     9721 ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc
     9781 accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag
     9841 gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc
     9901 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc
     9961 acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacat
//
