LOCUS       sequence-443649-        9252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is expressed FLT3 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9252)
  AUTHORS   Shin JE, Kim SH, Kong M, Kim HR, Yoon S, Kee KM, Kim JA, Kim DH, 
            Park SY, Park JH, Kim H, No KT, Lee HW, Gee HY, Hong S, Guan KL, Roe
            JS, Lee H, Kim DW, Park HW
  TITLE     Targeting FLT3-TAZ signaling to suppress drug resistance in blast 
            phase chronic myeloid leukemia.
  JOURNAL   Mol Cancer. 2023 Nov 6;22(1):177. doi: 10.1186/s12943-023-01837-4.
  PUBMED    37932786
REFERENCE   2  (bases 1 to 9252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Cancer."; date: "2023-11-6"; volume: "22(1):177. doi"; pages: " 
            10.1186/s12943-023-01837-4"
COMMENT     Sequence Label: pMSCV_FLT3
FEATURES             Location/Qualifiers
     source          1..9252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             177..693
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    757..1098
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1165..1581
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1511..1533
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1549..1565
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        4604..5103
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4639..4662)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5036..5055
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5124..5723
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5124..5143)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5620..5640
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5823..6337
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6499..6515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6523..6529,6530..6547,6548..6553))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6568..6589
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6706..6723)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6877..7465)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6957..6976)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7636..8427,8428..8496))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8259..8278
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8497..8601)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8669..8687
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8725..8747)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8847..8866
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9060..9082
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9210..9229
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ccggtgatgc cggccacgat gcgtccggcg tagaggcgat tagtccaatt tgttaaagac
       61 aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata
      121 gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
      181 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
      241 aaaatacata actgagaata gagaagttca gatcaaggtt aggaacagag agacagcaga
      301 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
      361 agatggtccc cagatgcggt cccgccctca gcagtttcta gagaaccatc agatgtttcc
      421 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc
      481 ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct
      541 cactcggcgc gccagtcctc cgatagactg cgtcgcccgg gtacccgtat tcccaataaa
      601 gcctcttgct gtttgcatcc gaatcgtgga ctcgctgatc cttgggaggg tctcctcaga
      661 ttgattgact gcccacctcg ggggtctttc atttggaggt tccaccgaga tttggagacc
      721 cctgcccagg gaccaccgac ccccccgccg ggaggtaagc tggccagcgg tcgtttcgtg
      781 tctgtctctg tctttgtgcg tgtttgtgcc ggcatctaat gtttgcgcct gcgtctgtac
      841 tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga gttctgaaca
      901 cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg tggcccgacc
      961 tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct ggtaggagac
     1021 gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg gaaccgaagc
     1081 cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg tctgactgtg
     1141 tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag tttgacctta
     1201 ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt caagaagaga
     1261 cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg gccgcgagac
     1321 ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc acctggcccg
     1381 catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc ttttgacccc
     1441 cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc tccatccgcc
     1501 ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct ttatccagcc
     1561 ctcactcctt ctctaggcgc cggaattcgt taaccatgcc ggcgttggcg cgcgacggcg
     1621 gccagctgcc gctgctcgtt gttttttctg caatgatatt tgggactatt acaaatcaag
     1681 atctgcctgt gatcaagtgt gttttaatca atcataagaa caatgattca tcagtgggga
     1741 agtcatcatc atatcccatg gtatcagaat ccccggaaga cctcgggtgt gcgttgagac
     1801 cccagagctc agggacagtg tacgaagctg ccgctgtgga agtggatgta tctgcttcca
     1861 tcacactgca agtgctggtc gatgccccag ggaacatttc ctgtctctgg gtctttaagc
     1921 acagctccct gaattgccag ccacattttg atttacaaaa cagaggagtt gtttccatgg
     1981 tcattttgaa aatgacagaa acccaagctg gagaatacct actttttatt cagagtgaag
     2041 ctaccaatta cacaatattg tttacagtga gtataagaaa taccctgctt tacacattaa
     2101 gaagacctta ctttagaaaa atggaaaacc aggacgccct ggtctgcata tctgagagcg
     2161 ttccagagcc gatcgtggaa tgggtgcttt gcgattcaca gggggaaagc tgtaaagaag
     2221 aaagtccagc tgttgttaaa aaggaggaaa aagtgcttca tgaattattt gggacggaca
     2281 taaggtgctg tgccagaaat gaactgggca gggaatgcac caggctgttc acaatagatc
     2341 taaatcaaac tcctcagacc acattgccac aattatttct taaagtaggg gaacccttat
     2401 ggataaggtg caaagctgtt catgtgaacc atggattcgg gctcacctgg gaattagaaa
     2461 acaaagcact cgaggagggc aactactttg agatgagtac ctattcaaca aacagaacta
     2521 tgatacggat tctgtttgct tttgtatcat cagtggcaag aaacgacacc ggatactaca
     2581 cttgttcctc ttcaaagcat cccagtcaat cagctttggt taccatcgta gaaaagggat
     2641 ttataaatgc taccaattca agtgaagatt atgaaattga ccaatatgaa gagttttgtt
     2701 tttctgtcag gtttaaagcc tacccacaaa tcagatgtac gtggaccttc tctcgaaaat
     2761 catttccttg tgagcaaaag ggtcttgata acggatacag catatccaag ttttgcaatc
     2821 ataagcacca gccaggagaa tatatattcc atgcagaaaa tgatgatgcc caatttacca
     2881 aaatgttcac gctgaatata agaaggaaac ctcaagtgct cgcagaagca tcggcaagtc
     2941 aggcgtcctg tttctcggat ggatacccat taccatcttg gacctggaag aagtgttcag
     3001 acaagtctcc caactgcaca gaagagatca cagaaggagt ctggaataga aaggctaaca
     3061 gaaaagtgtt tggacagtgg gtgtcgagca gtactctaaa catgagtgaa gccataaaag
     3121 ggttcctggt caagtgctgt gcatacaatt cccttggcac atcttgtgag acgatccttt
     3181 taaactctcc aggccccttc cctttcatcc aagacaacat ctcattctat gcaacaattg
     3241 gtgtttgtct cctcttcatt gtcgttttaa ccctgctaat ttgtcacaag tacaaaaagc
     3301 aatttaggta tgaaagccag ctacagatgg tacaggtgac cggctcctca gataatgagt
     3361 acttctacgt tgatttcaga gaatatgaat atgatctcaa atgggagttt ccaagagaaa
     3421 atttagagtt tgggaaggta ctaggatcag gtgcttttgg aaaagtgatg aacgcaacag
     3481 cttatggaat tagcaaaaca ggagtctcaa tccaggttgc cgtcaaaatg ctgaaagaaa
     3541 aagcagacag ctctgaaaga gaggcactca tgtcagaact caagatgatg acccagctgg
     3601 gaagccacga gaatattgtg aacctgctgg gggcgtgcac actgtcagga ccaatttact
     3661 tgatttttga atactgttgc tatggtgatc ttctcaacta tctaagaagt aaaagagaaa
     3721 aatttcacag gacttggaca gagattttca aggaacacaa tttcagtttt taccccactt
     3781 tccaatcaca tccaaattcc agcatgcctg gttcaagaga agttcagata cacccggact
     3841 cggatcaaat ctcagggctt catgggaatt catttcactc tgaagatgaa attgaatatg
     3901 aaaaccaaaa aaggctggaa gaagaggagg acttgaatgt gcttacattt gaagatcttc
     3961 tttgctttgc atatcaagtt gccaaaggaa tggaatttct ggaatttaag tcgtgtgttc
     4021 acagagacct ggccgccagg aacgtgcttg tcacccacgg gaaagtggtg aagatatgtg
     4081 actttggatt ggctcgagat atcatgagtg attccaacta tgttgtcagg ggcaatgccc
     4141 gtctgcctgt aaaatggatg gcccccgaaa gcctgtttga aggcatctac accattaaga
     4201 gtgatgtctg gtcatatgga atattactgt gggaaatctt ctcacttggt gtgaatcctt
     4261 accctggcat tccggttgat gctaacttct acaaactgat tcaaaatgga tttaaaatgg
     4321 atcagccatt ttatgctaca gaagaaatat acattataat gcaatcctgc tgggcttttg
     4381 actcaaggaa acggccatcc ttccctaatt tgacttcgtt tttaggatgt cagctggcag
     4441 atgcagaaga agcgatgtat cagaatgtgg atggccgtgt ttcggaatgt cctcacacct
     4501 accaaaacag gcgacctttc agcagagaga tggatttggg gctactctct ccgcaggctc
     4561 aggtcgaaga ttcgtagtta acctcgagag atctaattct accgggtagg ggaggcgctt
     4621 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     4681 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     4741 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     4801 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     4861 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     4921 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     4981 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     5041 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     5101 tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     5161 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     5221 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     5281 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     5341 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     5401 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     5461 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     5521 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     5581 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     5641 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     5701 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
     5761 gcgcacgacc ccatgcatcg ataaaataaa agattttatt tagtctccag aaaaaggggg
     5821 gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg
     5881 catggaaaat acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca
     5941 gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     6001 agaacagatg gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg
     6061 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     6121 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     6181 cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtgtatcca
     6241 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
     6301 ctgagtgatt gactacccgt cagcgggggt ctttcatggg taacagtttc ttgaagttgg
     6361 agaacaacat tctgagggta ggagtcgaat attaagtaat cctgactcaa ttagccactg
     6421 ttttgaatcc acatactcca atactcctga aatagttcat tatggacagc gcagaaagag
     6481 ctggggagaa ttgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6541 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6601 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6661 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6721 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6781 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6841 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6901 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6961 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7021 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7081 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7141 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7201 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7261 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7321 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7381 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7441 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7501 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7561 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7621 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7681 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7741 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7801 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7861 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7921 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7981 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8041 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8101 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8161 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8221 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8281 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8341 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8401 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8461 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8521 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8581 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     8641 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     8701 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     8761 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     8821 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     8881 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     8941 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     9001 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     9061 ccagtcacga cgttgtaaaa cgacggcgca aggaatggtg catgcaagga gatggcgccc
     9121 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
     9181 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
     9241 gcacctgtgg cg
//
