LOCUS       sequence-443652-        5016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancer-driven expression of the iCre(R297T) recombinase in 
            thalamic PVT neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5016)
  TITLE     Enhancer driven expression in thalamic populations
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AiP1999 - pAAV-AiE2255m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..5016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    16..127
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   245..385
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   245..374
                     /label=AAV2 ITR
     rep_origin      460..915
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(547..566)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     757..778
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(932..951)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1051..1073
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1111..1129)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1197..1301
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1302..1370,1371..2162)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1520..1539)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2333..2921
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2822..2841
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   2983..3112
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      3934..3943
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3946..3966
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcgagagatc ttcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
       61 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
      121 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
      181 gcaaggggga ggattgggaa gacaatagca ggcatgagat ctcacgtgcg gaccgagcgg
      241 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
      301 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
      361 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
      421 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
      481 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag
      541 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      601 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      661 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
      721 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
      781 caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
      841 tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
      901 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
      961 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     1021 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     1081 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     1141 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     1201 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1261 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1321 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1381 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1441 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1501 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     1561 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     1621 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     1681 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     1741 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     1801 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     1861 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     1921 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     1981 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2041 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2101 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2161 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2221 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2281 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2341 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2401 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2461 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2521 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2581 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2641 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2701 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2761 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2821 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2881 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     2941 ttttacggtt cctggccttt tgctggcctt ttgctcacat gtcctgcagg cagctgcgcg
     3001 ctcgctcgct cactgaggcc gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
     3061 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc ctgcggccgc
     3121 acgcgttgta agagtaataa tcgccggcgg gctgtgtttt attaaggcca tatcaactag
     3181 tttactgagt cacaccctgt atccgcttgc tagctcccct ccttcagtca aaggtgccac
     3241 ggtttagatc tttggatgga tgatgttatc tctggctccc ttgcctcggc agcaggtttt
     3301 ctgggaccca gtcctcagag caaatgagca gatcagaggt tctcacattg ttagagcttg
     3361 gctccacgtt gcccaattat ccccccagaa cgaaggcatt ggtagagcag gatctgcagg
     3421 tggctgagtt tctcatttgc tgccctgcag gtgtcttagc gcttgagctt ctggggaagt
     3481 ggcctctccc ctctctgtaa acagctgacc cagagaggaa gatgctgaga ccaggcattt
     3541 ctgagaccat gcagggtggg gacatgcacc cgtcctcatc tctgcaggac cctccacagg
     3601 gcggcacaag ccacgtgctc cgcaggggct cagctgggca gcattcctga gctgctggga
     3661 agaggggcca tccccagcta tctttcccat gtggtcctga gtcatccagt tccagtcttc
     3721 cctgtaggat tccttggggt ctcagagagc aaagctcggc aagcacaggg ccttggcagc
     3781 ttctctttgt ctttacagaa gggtggagct gatgggaaca gatgtgtgat ctgcttctct
     3841 gaggcgccgg cggagctcgg gctgggcata aaagtcaggg cagagccatc tattgcttac
     3901 atttgcttct gggatccaga tctttcgaag ctagccacca tggtgcccaa gaagaagagg
     3961 aaagtctcca acctgctgac tgtgcaccaa aacctgcctg ccctccctgt ggatgccacc
     4021 tctgatgaag tcaggaagaa cctgatggac atgttcaggg acaggcaggc cttctctgaa
     4081 cacacctgga agatgctcct gtctgtgtgc agatcctggg ctgcctggtg caagctgaac
     4141 aacaggaaat ggttccctgc tgaacctgag gatgtgaggg actacctcct gtacctgcaa
     4201 gccagaggcc tggctgtgaa gaccatccaa cagcacctgg gccagctcaa catgctgcac
     4261 aggagatctg gcctgcctcg cccttctgac tccaatgctg tgtccctggt gatgaggaga
     4321 atcagaaagg agaatgtgga tgctggggag agagccaagc aggccctggc ctttgaacgc
     4381 actgactttg accaagtcag atccctgatg gagaactctg acagatgcca ggacatcagg
     4441 aacctggcct tcctgggcat tgcctacaac accctgctgc gcattgccga aattgccaga
     4501 atcagagtga aggacatctc ccgcaccgat ggtgggagaa tgctgatcca cattggcagg
     4561 accaagaccc tggtgtccac agctggtgtg gagaaggccc tgtccctggg ggttaccaag
     4621 ctggtggaga gatggatctc tgtgtctggt gtggctgatg accccaacaa ctacctgttc
     4681 tgccgggtca gaaagaatgg tgtggctgcc ccttctgcca cctcccaact gtccacccgg
     4741 gccctggaag ggatctttga ggccacccac cgcctgatct atggtgccaa ggatgactct
     4801 gggcagagat acctggcctg gtctggccac tctgccagag tgggtgctgc caccgacatg
     4861 gccagggctg gtgtgtccat ccctgaaatc atgcaggctg gtggctggac caatgtgaac
     4921 attgtgatga actacatcag aaacctggac tctgagactg gggccatggt gaggctgctc
     4981 gaagatgggg actgaggcgc gccgaattca agcttc
//
