LOCUS       sequence-443659-        8705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pEF-HA-3xNES-CloningSite-mCherry-puroR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8705)
  AUTHORS   Liila-Fogarty SM, Boyum GE, Schwabe CL, Hess GT
  TITLE     A High-Throughput Screen for Antiproliferative Peptides in Mammalian
            Cells Identifies Key Transcription Factor Families.
  JOURNAL   ACS Synth Biol. 2024 Oct 19. doi: 10.1021/acssynbio.4c00337.
  PUBMED    39425622
REFERENCE   2  (bases 1 to 8705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Oct 19. doi: 10.1021/acssynbio.4c00337."
COMMENT     Sequence Label: pSL187
FEATURES             Location/Qualifiers
     source          1..8705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        58..256
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     211..231
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             274..454
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    501..626
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1123..1356
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1541..1585
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1883..2000
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2123..2334
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2335..3273
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3230..3250
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3296..3322
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3335..3361
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             3374..3400
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             3413..3439
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             3497..3550
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3551..4258
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3696..3714)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3964..3983
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4268..4324
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4325..4924
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4325..4344)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4821..4841
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(4944..4977)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5033..5621
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5086..5106)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5504..5515)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5624..5640)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5625..5641)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6150..6330
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6392..6980)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6472..6491)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7151..7942,7943..8011))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7774..7793
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8012..8116)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8191..8210)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8382..8705,1..56)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
       61 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      121 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      181 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      241 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
      301 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      361 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      421 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      481 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      541 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      601 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      661 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      721 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      781 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
      841 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
      901 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
      961 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1021 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1081 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1141 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1201 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1261 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1321 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1381 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1441 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1501 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1561 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1621 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1681 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1741 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1801 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     1861 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     1921 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     1981 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2041 taccgggccc gctctagaga tcccatttga ctcaggcggc cgcgtggata accgtattac
     2101 cgccatgcat gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     2161 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     2221 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     2281 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
     2341 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
     2401 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
     2461 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
     2521 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
     2581 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
     2641 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
     2701 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
     2761 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
     2821 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
     2881 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     2941 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     3001 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     3061 gattagttct cgcgcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     3121 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     3181 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     3241 acagtggttc aaagtttttt tcttccattt caggtgtcgt gagctagcca ccatgtatcc
     3301 ctatgacgtg cccgattatg ccggctcact tcaactgcct ccacttgaaa gactgacact
     3361 gggatcatta caattacctc ctttagaacg attaacactc ggttcactac agcttccgcc
     3421 tcttgagaga ttgacattag cggccgctgg atccggatca ggctccggag acgggtcaag
     3481 cgtctctgga tctggagagg gaaggggaag cctcctaact tgcggagatg tcgaggagaa
     3541 tcctggccca atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     3601 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     3661 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     3721 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     3781 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     3841 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     3901 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     3961 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     4021 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     4081 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     4141 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     4201 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggg
     4261 aagcggagct actaacttca gcctgctgaa gcaggctggt gacgtggagg agaaccctgg
     4321 acctatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     4381 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     4441 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     4501 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     4561 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     4621 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     4681 ggagcccgcg tggttcctgg ccaccgtcgg cgtatcgccc gaccaccagg gcaagggtct
     4741 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     4801 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     4861 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     4921 ctaagaattc gtcgagggac ctaataactt cgtatagcat acattatacg aagttataca
     4981 tgtttaaggg ttccggttcc actaggtaca attcgatatc aagcttatcg ataatcaacc
     5041 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     5101 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     5161 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     5221 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     5281 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     5341 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     5401 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     5461 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     5521 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     5581 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     5641 atcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     5701 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     5761 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     5821 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     5881 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
     5941 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
     6001 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
     6061 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
     6121 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
     6181 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6241 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6301 ccttttagtc agtgtggaaa atctctagca gcatgtgagc aaaaggccag caaaaggcca
     6361 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6421 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6481 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6541 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6601 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6661 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6721 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6781 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6841 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6901 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6961 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7021 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7081 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7141 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7201 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7261 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7321 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7381 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7441 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7501 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7561 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7621 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7681 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7741 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7801 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7861 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7921 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     7981 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8041 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8101 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     8161 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     8221 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     8281 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     8341 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     8401 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     8461 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     8521 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     8581 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     8641 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     8701 gacct
//
