LOCUS       sequence-44368-e       15613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15613)
  AUTHORS   Madisen L, Mao T, Koch H, Zhuo JM, Berenyi A, Fujisawa S, Hsu YW, 
            Garcia AJ 3rd, Gu X, Zanella S, Kidney J, Gu H, Mao Y, Hooks BM, 
            Boyden ES, Buzsaki G, Ramirez JM, Jones AR, Svoboda K, Han X, Turner
            EE, Zeng H
  TITLE     A toolbox of Cre-dependent optogenetic transgenic mice for 
            light-induced activation and silencing.
  JOURNAL   Nat Neurosci. 2012 Mar 25;15(5):793-802. doi: 10.1038/nn.3078.
  PUBMED    22446880
REFERENCE   2  (bases 1 to 15613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn"; 
            journalName: "Nat Neurosci"; date: "2012-03-25- 25"; volume: "15"; 
            issue: "5"; pages: "793-802"
COMMENT     Sequence Label: Ai35
FEATURES             Location/Qualifiers
     source          1..15613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..1087
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     enhancer        1181..1484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1486..1763
                     /label=chicken beta-actin promoter
     intron          1765..2782
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2706..2728
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2790..2809
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2858..2891
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    2915..2948
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3116..3250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3370..3504
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3624..3758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3787..3820
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3949..3958
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4024..4794
                     /codon_start=1
                     /product="light-driven proton pump archaerhodopsin-3 from 
                     Halorubrum sodomense (Chow et al., 2010)"
                     /label=Arch
                     /translation="DPIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVTD
                     KDAREYYAVTILVPGIASAAYLSMFFGIGLTEVTVGGEMLDIYYARYADWLFTTPLLLL
                     DLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYFLAT
                     SLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLDV
                     TAKVGFGFILLRSRAILGDTEAPEPSAGADVSAAD"
     CDS             join(4810..4812,4813..5523)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4810..4812,4813..5523)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4852..4873)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5113..5132)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5460..5481
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5675..6263
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5728..5748)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6146..6157)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6266..6282)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6267..6283)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6278..6295)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6284..6491
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6499..6532
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        6554..7053
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6589..6612)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6986..7005
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     protein_bind    7073..7106
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      7107..7116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7113..7916
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7176..7195)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7786..7805
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7926..8387
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     misc_feature    8451..12720
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(12765..12785)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12765..12783)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12804..12820)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12804..12820)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12817..12839)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12828..12844
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12852..12858,12859..12876,12877..12882))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12897..12918
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13035..13052)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13206..13794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13286..13305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13965..14756,14757..14825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14588..14607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14826..14930)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(14956..15411)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15093..15114)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15305..15324
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15538..15560
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15552..15569
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15553..15569
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15579..15598
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15579..15597
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 ggggcagcgg aggascgctt ccggccgacg tctcgtcgct gattggctty ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctgn acgtgcgggc gcggtcggcc tctggcgggg cgggggaggg
      421 gagggagggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
      481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
      541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
      601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
      661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
      721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
      781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
      841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
      901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
      961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
     1021 cgccctgggc ctgggagaat cccttgcccc ctcttcccct cgtgatctgc aactccagtc
     1081 tttctagtta attaaccgtt taaacaattc tgcaggaatc tagttattaa tagtaatcaa
     1141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1441 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     1501 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     1561 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
     1621 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
     1681 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
     1741 aaaagcgaag cgcgcggcgg gcggggagtc gctgcgacgc tgccttcgcc ccgtgccccg
     1801 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
     1861 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct
     1921 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg
     1981 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc
     2041 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag
     2101 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg
     2161 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc
     2221 ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc
     2281 gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg
     2341 gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg
     2401 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct
     2461 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc
     2521 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc
     2581 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct
     2641 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag
     2701 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     2761 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
     2821 ttttggcaaa gaattgattt gataccgcgg gccctaagaa gttcctattc tctagaaagt
     2881 ataggaactt cgtcgacatt taaatcattt aaatataact tcgtataatg tatgctatac
     2941 gaagttattc gcgatgaata aatgaaagct tgcagatctg cgactctaga ggatctgcga
     3001 ctctagagga tcataatcag ccntaccaca ttttgtagag gttttactng ctttaaaaaa
     3061 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     3121 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3181 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     3241 tgtctggatc tgcgactcta gaggatcata atcagccata ccacatttgt agaggtttta
     3301 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     3361 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3421 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3481 aatgtatctt atcatgtctg gatctgcgac tctagaggat cataatcagc cataccacat
     3541 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     3601 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     3661 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3721 tgtccaaact catcaatgta tcttatcatg tctggatccc catcaagctg atccggaacc
     3781 cttaatataa cttcgtataa tgtatgctat acgaagttat taggtccctc gacctgcagc
     3841 ccaagctaga tcgaattcgg ccggccttgt acgcgtgtca atagcccaag atggccgcct
     3901 ttaaataggc cggccctttc tgcagcttcc actaaattgt cgagatccgc caccatggtc
     3961 ccgtgcacgc tgctcctgct gttggcagcc gccctggctc cgactcagac gcgggccagc
     4021 gctgacccca tcgctctgca ggctggttac gacctgctgg gtgacggcag acctgaaact
     4081 ctgtggctgg gcatcggcac tctgctgatg ctgattggaa ccttctactt tctggtccgc
     4141 ggatggggag tcaccgataa ggatgcccgg gaatattacg ctgtgactat cctggtgccc
     4201 ggaatcgcat ccgccgcata tctgtctatg ttctttggta tcgggcttac tgaggtgacc
     4261 gtcgggggcg aaatgttgga tatctattat gccaggtacg ccgactggct gtttaccacc
     4321 ccacttctgc tgctggatct ggcccttctc gctaaggtgg atcgggtgac catcggcacc
     4381 ctggtgggtg tggacgccct gatgatcgtc actggcctca tcggagcctt gagccacacg
     4441 gccatagcca gatacagttg gtggttgttc tctacaattt gcatgatagt ggtgctctat
     4501 tttctggcta catccctgcg atctgctgca aaggagcggg gccccgaggt ggcatctacc
     4561 tttaacaccc tgacagctct ggtcttggtg ctgtggaccg cttaccctat cctgtggatc
     4621 ataggcactg agggcgctgg cgtggtgggc ctgggcatcg aaactctgct gtttatggtg
     4681 ttggacgtga ctgccaaggt cggctttggc tttatcctgt tgagatcccg ggctattctg
     4741 ggcgacaccg aggcaccaga acccagtgcc ggtgccgatg tcagtgccgc cgaccgaccg
     4801 gtagtagcag tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     4861 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     4921 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     4981 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     5041 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     5101 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     5161 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     5221 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     5281 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     5341 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     5401 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     5461 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     5521 aagttctgct acgagaatga agtgtaagaa ttcctaacta agccggtact gtaactagtg
     5581 ttatcctgtt tagggatatc ttgggctatt gacacgcgtt aagtgcaaca cgtgaaggcc
     5641 ggccctgcag gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     5701 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     5761 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     5821 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     5881 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     5941 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     6001 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     6061 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     6121 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     6181 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     6241 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgactgtgcc ttctagttgc
     6301 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     6361 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     6421 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     6481 catgctgggg aactagtggt gccagggcgt gcccttgggc tccccgggcg cggcggccgc
     6541 atcgaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     6601 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     6661 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     6721 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     6781 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     6841 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     6901 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     6961 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     7021 ctgttctcct cttcctcatc tccgggcctt tcgacctgca atcgccgcta gcgaagttcc
     7081 tattctctag aaagtatagg aacttcgcca ccatgggatc ggccattgaa caagatggat
     7141 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     7201 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     7261 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     7321 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     7381 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     7441 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     7501 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     7561 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     7621 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga
     7681 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     7741 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     7801 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     7861 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagggg
     7921 atccgctgta agtctgcaga aattgatgat ctattaaaca ataaagatgt ccactaaaat
     7981 ggaagttttt cctgtcatac tttgttaaga agggtgagaa cagagtacct acattttgaa
     8041 tggaaggatt ggagctacgg gggtgggggt ggggtgggat tagataaatg cctgctcttt
     8101 actgaaggct ctttactatt gctttatgat aatgtttcat agttggatat cataatttaa
     8161 acaagcaaaa ccaaattaag ggccagctca ttcctcccac tcatgatcta tagatctata
     8221 gatctctcgt gggatcattg tttttctctt gattcccact ttgtggttct aagtactgtg
     8281 gtttccaaat gtgtcagttt catagcctga agaacgagat cagcagcctc tgttccacat
     8341 acacttcatt ctcagtattg ttttgccaag ttctaattcc atcagaaagc ttgcagaaga
     8401 tctccccaac tggggtaacc tttgagttct ctcagttggg ggggcgcgcc ctagaagatg
     8461 ggcgggagtc ttctgggcag gcttaaaggc taacctggtg tgtgggcgtt gtcctgcagg
     8521 ggaattgaac aggtgtaaaa ttggagggac aagacttccc acagattttc ggttttgtcg
     8581 ggaagttttt taataggggc aaataggaaa atggaggata ggagtcatct ggggtttatg
     8641 cagcaaaact acaggtatat tgcttgtatc cgcctcggag atttccatga ggagataaag
     8701 acatgtcacc cgagtttata ctctcctgct tagatcctac tacagtatga aatacagtgt
     8761 ygcgaggtag actatgtaag cagatttaat cattttaaag agcccagtac ttcatatcca
     8821 tttctcccgc tccttctgca gccttatcaa aaggtattta gaacactcat tttagcccca
     8881 ttttcattta ttatactggc ttatccaacc cctagacaga gcattggcat tttccctttc
     8941 ctgatcttag aagtctgatg actcatgaaa ccagacagat tagttacata caccacaaat
     9001 cgaggctgta gctggggcct caacactgca gttcttttat aactccttag tacacttttt
     9061 gttgatcctt tgccttgatc cttaattttc agtgtctatc acctctcccg tcaggtggtg
     9121 ttccacattt gggcctattc tcagtccagg gagttttaca acaatagatg tattgagaat
     9181 ccaacctaaa gcttaacttt ccactcccat gaatgcctct ctcctttttc tccattataa
     9241 ctgagctatw accattaatg gtttcaggtg gatgtctcct cccccaatat acctgatgta
     9301 tctacatatt gccaggctga tattttaaga catwaaaggt atatttcatt attgagccac
     9361 atggtattga ttactgctac taaaattttg tcattgtaca catctgtaaa aggtggttcc
     9421 ttttggaatg caaagttcag gtgtttgttg tctttcctga cctaaggtct tgtgagcttg
     9481 tattttttct atttaagcag tgctttctct tggactggct tgactcatgg cattctacac
     9541 gttattgctg gtctaaatgt gattttgcca agcttcttca ggacctataa ttttgcttga
     9601 cttgtagcca aacacaagta aaatgattaa gcaacaaatg tatttgtgaa gcttggtttt
     9661 taggttgttg tgttgtgtgt gcttgtgctc tataataata ctatccaggg gctggagagg
     9721 tggctcggag ttcaagagca cagactgctc ttccagaagt cctgagttca attcccagca
     9781 accacatggt ggctcacaac catctgtaat gggatctgat gccctcttct ggtgtgtctg
     9841 aagaccacaa gtgtattcac attaaataaa taatcctcct tcttcttctt tttttttttt
     9901 taaagagaat wctgtctcca gtagaattac tgaagtaatg aaatactttg tgtttgttcc
     9961 aatatggwag ccaataatca aatactcttw agcactggaa atgtaccaag gaactatttt
    10021 atttaagtgw actgtggaca gaggagccat aactgcagac ttgtgggata cagaagacca
    10081 atgcagactt aatgtctttt ctcttacact aagcaataaa gaaataaaaa ttgaacttct
    10141 agtatcctat ttgttaaact gctagcttta ctaacttttg tgcttcatct atacaaagct
    10201 gaaagctaag tctgcagcca ttactaaaca tgaaagcaag taatgataat tttggatttc
    10261 aaaaatgtag ggccagagtt tagccagcca gtggtggtgc ttgcctttat gccttaatcc
    10321 cagcactctg gaggcagaga caggcagatc tctgagtttg agcccagcct ggtctacaca
    10381 tcaagttcta tctaggatag ccaggaatac acacagaaac cctgttgggg aggggggctc
    10441 tgagatttca taaaattata attgaagcat tccctaatga gccactatgg atgtggctaa
    10501 atccgtctac ctttctgatg agatttgggt attatttttt ctgtctctgc tgttggttgg
    10561 gtcttttgac actgtgggct ttcttaaagc ctccttccct gccatgtggt ctcttgtttg
    10621 ctactaactt cccatggctt aaatggcatg gctttttgcc ttctaagggc agctgctgag
    10681 wtttgcagcc tgatttccag ggtggggttg ggaaatcttt caaacactaa aattgtcctt
    10741 taattttttt ttaaaaaatg ggttatataa taaacctcat aaaatagtta tgaggagtga
    10801 ggtggactaa tattaatgag tccctcccct ataaaagagc tattaaggct ttttgtctta
    10861 tactaacttt ttttttaaat gtggtatctt tagaaccaag ggtcttagag ttttagtata
    10921 cagaaactgt tgcatcgctt aatcagattt tctagtttca aatccagaga atccaaattc
    10981 ttcacagcca aagtcaaatt aagaatttct gactttaatg ttatttgcta ctgtgaatat
    11041 aaaatgatag cttttcctga ggcagggtct cactatgtat ctctgcctga tctgcaacaa
    11101 gatatgtaga ctaaagttct gcctgctttt gtctcctgaa tactaaggtt aaaatgtagt
    11161 aatacttttg gaacttgcag gtcagattct tttatagggg acacactaag ggagcttggg
    11221 tgatagttgg taaatgtgtt taagtgatga aaacttgaat tattatcacc gcaacctact
    11281 ttttaaaaaa aaaagccagg cctgttagag catgctaagg gatccctagg acttgctgag
    11341 cacacaagag tagtacttgg caggctcctg gtgagagcat atttcaaaaa acaaggcaga
    11401 caaccaagaa actacagtaa ggttacctgt ctttaaccat ctgcatatac acagggatat
    11461 taaaatattc caaataatat ttcattcaag ttttccccca tcaaattggg acatggattt
    11521 ctccggtgaa taggcagagt tggaaactaa acaaatgttg gttttgtgat ttgtgaaatt
    11581 gttttcaagt gatagttaaa gcccatgaga tacagaacaa agctgctatt tcgaggtctc
    11641 ttggttatac tcagaagcac ttctttgggt ttccctgcac tatcctgatc atgtgctagg
    11701 cctwccttag gctgattgtt gttcaaataa cttaagtttc ctgtcaggtg atgtcatatg
    11761 atttcatata tcaaggcaaa acatgttata tatgttaaac atttgkactt aatgtgaaag
    11821 ttaggtcttt gtgggttttg attttaattt caaaacctga gctaaataag tcattttaca
    11881 tgtcttacat ttggtgaatt gtatattgtg gtttgcaggc aagactctct gacctagtaa
    11941 ccctcctata gagcactttg ctgggtcaca agtctaggag tcaagcattt caccttgaag
    12001 ttgagacgtt ttgttagtgt atactagtta tatgttggag gacatgttta tccagaagat
    12061 attcaggact atttttgact gggctaagga attgattctg attagcactg ttagtgagca
    12121 ttgagtggcc tttaggcttg aattggagtc acttgtatat ctcaaataat gctggccttt
    12181 tttwaaaagc ccttgttctt tatcaccctg ttttctacat aatttttgtt caaagaaata
    12241 cttgtttgga tctccttttg acaacaatag catgttttca agccatattt tttttccttt
    12301 tttttttttt ttttggtttt tcgagacagg gtttctctgt atagccctgg ctgtcctgga
    12361 actcactttg tagaccaggc tggcctcgaa ctcagaaatc cgcctgcctc tgcctcctga
    12421 gtgccgggat taaaggcgtg caccaccacg cctggctaag ttggatattt tgtatataac
    12481 tataaccaat actaactcca ctgggtggat ttttaattca gtcagtagtc ttaagtggtc
    12541 tttattggcc cttattaaaa tctactgttc actctaacag aggctgttgg actagtggsa
    12601 ctaagcaact tcctacggat atactagcag ataagggtca gggatagaaa ctagtctagc
    12661 gttttgtata cctaccagct tatactacct tgttctgata gaaatattta ggacatctag
    12721 cttatcgatc cgtcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta
    12781 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    12841 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    12901 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    12961 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    13021 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    13081 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    13141 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    13201 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    13261 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    13321 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    13381 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    13441 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    13501 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    13561 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    13621 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
    13681 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    13741 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    13801 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    13861 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    13921 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    13981 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    14041 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    14101 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    14161 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    14221 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    14281 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    14341 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    14401 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    14461 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    14521 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    14581 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    14641 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    14701 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    14761 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    14821 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    14881 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    14941 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
    15001 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
    15061 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
    15121 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
    15181 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
    15241 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
    15301 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
    15361 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
    15421 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
    15481 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
    15541 agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg
    15601 aattggagct cca
//
