LOCUS       sequence-443702-        6410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Komori C, Takahashi T, Nakano Y, Ui-Tei K
  TITLE     TRBP-Dicer interaction may enhance HIV-1 TAR RNA translation via TAR
            RNA processing, repressing host-cell apoptosis.
  JOURNAL   Biol Open. 2020 Feb 25;9(2):bio050435. doi: 10.1242/bio.050435.
  PUBMED    32051109
REFERENCE   2  (bases 1 to 6410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Open."; date: "2020-02-25"; volume: "9(2):bio050435. doi"; pages: " 
            10.1242/bio.050435"
COMMENT     Sequence Label: pGL2-deltaTAR-luciferase
FEATURES             Location/Qualifiers
     source          1..6410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             751..2403
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(817..835)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2644..2709
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2839..2859
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3284..3418
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3560..3577)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3731..4319)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3811..3830)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4490..5281,5282..5350))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5113..5132
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5351..5455)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5482..5937
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5569..5588)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5779..5800
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    6127..6248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aaacacaaaa attagctggg catggtggtg ggcacctgta atcccagcta ttcaggaggc
       61 tgaggcacaa gaattgcttg aacccaggag gcggagattg cagtgagcca agattgtgcc
      121 actgcactcc agcctgggca acaagagcaa aactctatct caaaaaaaaa aaaaaaaaaa
      181 aaaatttaat tttaagagcc tgaaaagtcg aagccaaggc aggcagaaga gatagtcaca
      241 tcacatatgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
      301 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc aggggtcaga
      361 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
      421 gaagccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tggaatggat
      481 gaccctgaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
      541 gcccgagagc tgcatccgga gtacttcaag aactgctgac atcgagcttg ctacaaggga
      601 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
      661 ctcagatgct gcatataagc agctgctttt tgcctgtact agtactgctt aagcctcaat
      721 aaagcttggc attccggtac tgttggtaaa atggaagacg ccaaaaacat aaagaaaggc
      781 ccggcgccat tctatcctct agaggatgga accgctggag agcaactgca taaggctatg
      841 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtgaac
      901 atcacgtacg cggaatactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat
      961 gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg
     1021 ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat
     1081 gaacgtgaat tgctcaacag tatgaacatt tcgcagccta ccgtagtgtt tgtttccaaa
     1141 aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac caataatcca gaaaattatt
     1201 atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct
     1261 catctacctc ccggttttaa tgaatacgat tttgtaccag agtcctttga tcgtgacaaa
     1321 acaattgcac tgataatgaa ttcctctgga tctactgggt tacctaaggg tgtggccctt
     1381 ccgcatagaa ctgcctgcgt cagattctcg catgccagag atcctatttt tggcaatcaa
     1441 atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt
     1501 actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa
     1561 gagctgtttt tacgatccct tcaggattac aaaattcaaa gtgcgttgct agtaccaacc
     1621 ctattttcat tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac
     1681 gaaattgctt ctgggggcgc acctctttcg aaagaagtcg gggaagcggt tgcaaaacgc
     1741 ttccatcttc cagggatacg acaaggatat gggctcactg agactacatc agctattctg
     1801 attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa
     1861 gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcagag aggcgaatta
     1921 tgtgtcagag gacctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc
     1981 ttgattgaca aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa
     2041 cacttcttca tagttgaccg cttgaagtct ttaattaaat acaaaggata tcaggtggcc
     2101 cccgctgaat tggaatcgat attgttacaa caccccaaca tcttcgacgc gggcgtggca
     2161 ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga
     2221 aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa
     2281 aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc
     2341 gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gtccaaattg
     2401 taaaatgtaa ctgtattcag cgatgacgaa attcttagct attgtaatac tgcgatgagt
     2461 ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttaaacg cctggttgct
     2521 acgcctgaat aagtgataat aagcggatga atggcagaaa ttcgccggat ctttgtgaag
     2581 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
     2641 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     2701 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
     2761 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
     2821 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
     2881 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
     2941 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
     3001 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
     3061 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
     3121 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
     3181 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     3241 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     3301 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     3361 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
     3421 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
     3481 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
     3541 ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3601 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3661 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3721 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3781 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3841 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3901 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3961 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4021 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4081 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4141 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     4201 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4261 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4321 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4381 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4441 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4501 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4561 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4621 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4681 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4741 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4801 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4861 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4921 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4981 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5041 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5101 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5161 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     5221 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5281 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5341 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5401 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5461 ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     5521 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5581 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     5641 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5701 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     5761 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     5821 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     5881 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttgcc
     5941 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     6001 tacgccagcc caagctacca tgataagtaa gtaatattaa ggtacgtgga ggttttactt
     6061 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     6121 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6181 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6241 gtatcttatg gtactgtaac tgagctaaca taacccaatt caattttggg ccaggtgagg
     6301 tggctcacgt ctgtaatctc agcactttgg gaggctgagg ggggtggatc acctgaggtc
     6361 aggagttcaa gaccagcctg gccaacatgg taaaaccctg tctctactaa
//
