LOCUS       sequence-44373-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbB0k-RFP
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             173..184
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(193..227)
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     primer_bind     complement(433..452)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 acggcagcgg accacctttg gtaactttca gtttagcggt ctgggtacct tcgtacggac
       61 gaccttcacc ttcaccttcg atttcgaact cgtgaccgtt aacggaacct tccatacgaa
      121 ctttgaaacg catgaactct ttgataacgt cttcggagga agccatctag tatttctcct
      181 ctttcactag tagctagcac agtacctagg actgagctag ctgtcaaaga tctcatgaat
      241 tcggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg
      301 atggctttct tgccgccaag gatctgatgg cgcaggggat catgatctga tcaagagaca
      361 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
      421 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
      481 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
      541 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
      601 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
      661 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
      721 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
      781 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
      841 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
      901 aaggcgcgca tgcccgacgg cgagacctct gccagcagcg tgcaaacagt
//
