LOCUS       sequence-443736-       11655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express YEATS4 gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 11655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:YEATS4_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..11655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    5828..5962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    5983..6117
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(6419..7132,7133..7135,7136..7138))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6422..7132,7133..7135,7136..7138))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6464..6485)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6813..6832
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7072..7093
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8319..8335)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8319..8335)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(8390..8408)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8418..8435)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8418..8434)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8427..8449)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(8575..9030)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8712..8733)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8924..8943
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9057..9161
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9162..9230,9231..10022)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9380..9399)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10193..10781
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10682..10701
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10935..10952
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11069..11090
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11105..11110,11111..11128,11129..11135)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11143..11159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11148..11170
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11167..11183
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11167..11183
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11202..11222
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11204..11222
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcactcgtgg ttacgggttt ttgttttgca ctcttgcaca cacatctctc ggcctccaaa
       61 acaaacacac aactgcacga gggctcgaag cccccaccga aaaacaaaaa cattatgcta
      121 atgaggaagg cacagctgtt cccttcttca ctctctctct cgttctttca acaacagtcg
      181 cctaatttct ctcgttcctt taaagagccg ccaagcagaa acagttaatg caaatataag
      241 cctgcgggaa ataaggagaa tttttacaaa atccgagctt cccctcctgc caaaatgtga
      301 tgtgtgactc acgcattcga tatttggtgt aatgagagtg aatcattctg ctccacaccg
      361 aacccgatgc cacagaccac tcagatgcac accgaggaga ctattttaac agcacattac
      421 atccagactg tcacagatgc attaccaaga gtgtgatttc agtgtcgaat gcaggtctta
      481 aaggaaaatc tgttgcgatt gcatgtgcgc taactagaca cgaccaacga atgggaacaa
      541 tctgtgaaat attatacaat cacaggcaat taaaagatgt ttaaagttca atttagtttt
      601 aattaatatg ttatataaaa ctaatctgtt ttaaatgtat atttatgatt ttagctttag
      661 ctagcctctt gaccagcagc tgaagtgtcc ggcaagtacc taaaagggag accacatggg
      721 aaagctaggt tgctcccgga agaggtgtta gtaaaccagc aggtctacct gtggtctgtg
      781 tgggtcctaa tgccccagta taaaccagca ggtcaacctg tggtctgtgt gggtcctaat
      841 gccccagtat aaaccagcag gtcaacctgt ggtctgtgtg ggtcctaatg ccccagtata
      901 ttgatggaga ctctatactg ctcggtgagc tccatctttc ggataagatg ttaaaacaag
      961 gtcccgactc tctgtggtgg taaaaaattc ccaggatgtc cttcctaccc tggcatcctg
     1021 gacaaatttg ctcactgtcc tctgtccatc atggcctcct aaccatcccc atatcataat
     1081 tggctttatc actctagctg gggccgaaat cgcatacttc cattttatga ggtacgctaa
     1141 aatcagtatg cgagccaagt aatatgtccg aatacttccg gcgagattct agagtgtgca
     1201 ttcgatgcac gctacgctat tccatgatgc cccgtgagag aattcttgaa tcggagagaa
     1261 gcaacgcaac tcatgcgggt aggttacgtg acgatgacat attggcggat gtagtacgtc
     1321 caagttccat tcatactgct cacattcata ttttatagaa tgttcttttc ccacagccaa
     1381 gtagtaagtt taaattcaaa tacagtacct actaagtagt agtagacggt ttcggacgca
     1441 gcccctgtct cctctccacc aatatactgg tgtgtagtgt gcggtctggt gcaatatggc
     1501 tgccatcgcg tcatccaggt ggatgctgca cactggtggt tgatgaggag attttgccca
     1561 aaaaagtgtt ttgagtttcc agaaaagcgc gatataaatg taaggaatta tcattagctt
     1621 attataatgg aactttctgg ataacttgtg ccagagctag gggccattaa aactttgccc
     1681 tggggtacct cagggagctg caaggaagtg ctatagggct aatgaaatgt ttgggggaaa
     1741 aaaaaaaatt ttataagtaa aaataaaact aaaataatta ataatttcat tgttgaaaat
     1801 tatagtaaaa ttgataaaca agataagcag cattagtata aaaaactaaa aacaaacgta
     1861 atttaaaata attaaattgt tttaaataaa ttatacaaaa taatgtttac aacgacacgg
     1921 tggtgcagta ggtagtgctg tcgccagcaa agcaagaagg tcgctggttc gagcctcagc
     1981 tggatcactt gatgtttctg tgtggagcat tcgcgtgcgt ttaaccccag attaataaag
     2041 agactaagcc gaaaagaaaa taaatgtatg aaaatgttta cgtaaaatgt aaataaatgc
     2101 atgattaata aaatatatga attttaaaga ttgaggtaga attgttttat tattcacaca
     2161 tttggttatt tttaatcagt gacaacttgt gatgctcttt atattgttta ttacgctact
     2221 ttgaatattc agtctcaaat tgcatgtaaa tacgacttca aagcagaatt agtttaattt
     2281 aattgcctgt aaacagtgtt gtactttgat ggtgattgtc tgataataat atgatttcac
     2341 tatttaaaat ttgaaaaaaa aaaatctgaa aaatactcac cggccacttt attaggtaca
     2401 cctgtctaac tgcttgttaa cgtaaatttc taatcagcca atcaccaggc agcaactcag
     2461 tgcatttagg catgtagaca tggtcaagac gatctgctgc agttcaaagc gagcatcaga
     2521 atggagaagc aaggtgattt aagtgacttt aaacgtgacg tgtttgttgg tgccagacgg
     2581 gctggtctga gtatttcaga aactgctgat ttactggggt tttcacacac aaacatctct
     2641 ggggtttaca gagaatggtc caaaaaaaga taatatccag tgagcggcag ttctgtgggg
     2701 gcaaatgtct tgttgatgcc agaggtcaga ggagaatggc ctgactggtt ccagctgata
     2761 gaaaggcaac agtaattcaa ataaccactc gttagaactg aggcatgcag aagagcacct
     2821 ttgaacacaa catgtcgaac cttgaggcgg gtgggctaca gcagctcaag accacatcgg
     2881 gtgccactcc tgtcagctaa gaacaggaaa ctgaggctac aattcgcaca ggctcaacat
     2941 aagtagacaa tagaagattg aacattatgt tgcctggtca gatggataca ttctaccttg
     3001 tatcaacagt tcaggctggt gatggtggtg taatggtgtg ggggatatat tcttggcaca
     3061 ctttgggccc attagtacca attaaccatc gtgtcaacgc cacagcctaa ctgagtattg
     3121 ttgctgacca tgttcatttc tttttgacca cagtgtaccc atcttctgat ggctacttcc
     3181 tgcaggataa tgcaccatgt cataaagcgt gaatgatctc agactggttt cttgaacatg
     3241 acaatgagtt cactgtgctc gaatgacctc cacagtcacc agatctcaat ccaatagagc
     3301 gcctttgggg tgtggtagaa tggaagattc gcatcctgga tgtgcagccg acaaatctgc
     3361 atgatgctag catgtcaata tggaccaaaa tctctgagga attttccagt acctggttga
     3421 aatgcaaaag gaagggaatc tatgctaagg caaaaagagg tccaacccag tactagtggg
     3481 gtgtacataa taaagtggcc ggtgagtgta tattgttaag gatatctaga taatccaaat
     3541 aatttacagc actatttaaa aaaaaaactt tatgtcacca agtctgcatt tatttgatca
     3601 caattacagt cgaaaaagca atatcgggat acattgtttc catttaaaat aactatatta
     3661 tattttaaat atttttgaca atgttatttc tttaatgcca aagctaaatt gtattcaatc
     3721 tgaattttag tgtcacatga tccttcagaa aatattaaca cattgctaaa aatgtatatt
     3781 attatcaatt tggatttttt tttttttttt gtatattaac ctatgctatt ggttactata
     3841 caaaagtatt actgtgaata tatgcatata cagaaccgta tcaagcgcgg ggggtggttg
     3901 ggttatgtcc ccatttggaa agacctggaa acattcataa tgactaaaat tgcacggaaa
     3961 aatataatat ctatgagtat tgctgatcag cagcacaaaa ccagaactcc atattaataa
     4021 acagccaatg atgcagtgag tttgtgcatg ttttcattca aactcgtgtg attgtgctca
     4081 aacacctgtt gtgagcagca gttgcaaaca gtaaacctca agaggagcca gtcaaccttc
     4141 acaaacactc aacctctggc tcactcacta aacaattcgc gttaatatct aaaataaaaa
     4201 tttccagcac caaacagctc tcagatttcg gagtacactg accaagaggt aagaagtaac
     4261 gttacattac taggacgact attgtaagac tagatatgta atatttggaa gcgtacgtgt
     4321 tttaagtgac atatagagta gaggaactga tggaaatacg atttgcataa tgtaaaatga
     4381 tatatattaa aatataggct acagtaggta acgtggttta catataacgt tactgtgtta
     4441 atgtagtgct cagttactcg tgttgacagt tattattagt gcttgctaat ttgtttgtta
     4501 atgcttctct tgcgtatttg attaccttca ggcgaatcta aaacatatca gatgtagaaa
     4561 cggcagaaaa atgttgaaga aaatacgaga atagtagctt ttggagcaca tagaaaagta
     4621 catcagataa agcctaccgt ttaaacgcag acgccgctct cgcactgaat gctgggtact
     4681 gtagtcctta tattgtatgc ttaatgtgcg tctcaacggg aaactagagt taggaaaact
     4741 acaatttcct gtggaaaatg tttctgtttt cggggaaaca gcgaaagaaa gatatggttc
     4801 cgcgtttttt tcttactttg aataataaaa taagacctaa ctacacactt cgcttactct
     4861 tatgctaatt tgaagcacta gcattctttg atacaatgat aatcatttaa acatcacagt
     4921 tatactacaa atactatggt taaactatgt ctagtgtagt gtaacactat ggttgccatt
     4981 gttatcttta agtttaagta ttatctgtgg taaagccatg gttggttttt ataagaggta
     5041 ataaaattaa cattgtacaa ttaaatgact ataaattaat gttttttttt atttttttta
     5101 cacagctacc ttcaaaaagg gatccgccgc caccatgttc aagagaatgg ccgaatttgg
     5161 gcctgactcc ggcgggagag taaagggtgt tactatcgtt aaaccaatag tttacggtaa
     5221 tgttgctcgg tattttggaa agaaaagaga agaagatggg cacactcatc agtggacagt
     5281 atatgtgaaa ccatatagaa atgaggatat gtcagcatat gtgaagaaaa tccagtttaa
     5341 attacatgaa agctatggca atcctttaag agttgttact aaacctccat atgaaattac
     5401 tgaaacagga tggggtgaat tcgaaataat catcaaaata tttttcattg accctaatga
     5461 aagacctgta accctgtatc atttgctaaa gctgtttcaa tcagacacca atgcaatgct
     5521 ggggaaaaag acagtggttt cagagttcta tgatgaaatg atatttcaag acccaacagc
     5581 aatgatgcaa caattattga caacatctcg tcagctaaca ttaggagcct ataagcatga
     5641 aacagaattt gcagagcttg aagtgaaaac cagagaaaaa ttagaagctg ctaagaaaaa
     5701 aacaagcttt gagattgcag agcttaagga gagattaaaa gcaagtcgtg aaactataaa
     5761 ttgtttaaaa aatgaaatca gaaaacttga agaagatgac caagcaaaag acatataaat
     5821 cgatgatgat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     5881 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     5941 tataagctgc aataaacaag ttaacaacaa caattcgatg atgatccaga catgataaga
     6001 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     6061 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     6121 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     6181 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga tcagatctct
     6241 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6301 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6361 atgtctggat ctacgtaata cgactcacta tagttctaga ggctcgagag gggccgcttt
     6421 acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
     6481 ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt
     6541 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
     6601 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt
     6661 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
     6721 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
     6781 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
     6841 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
     6901 tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg
     6961 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
     7021 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
     7081 ccagctcgac caggatgggc accaccccgg tgaacagctc ttcgcccttg ctcaccatgg
     7141 tcactgtctg ctttgctgtt ggtctgggct cctgggtcac tggcttacta atggagtctt
     7201 tatgtatgag gactcttatc aattgttctt ctataaaggt ctgcagtgtt tctgttcgtc
     7261 ccctacatgg acacccagag cctcctaaat acaggagccc tgataactgc acaagtgctc
     7321 agattccagc agggtggaaa tgagataaag tgtgcagatg gggaggggga cgtgaatgag
     7381 agatttgagg gatgaaaagg atggatgaac gcattgaaaa tagccccttt cacaaaataa
     7441 taccagtaaa ttgtcatata attaatgacc agacctttac ctgtaaatga tgtgatgttc
     7501 atatacagaa gaatgccaac aatttacaga tgattttaca aattcttaat tttccttttc
     7561 taaatggatt tgttcacaca ttatctcttc aaagcaattg aattttctgg aaaagattgg
     7621 ctgtgtgtgt aaaaggtgct actgaggaat ttaatgtgac atggaatgaa gcaaacagca
     7681 gcctaagaca ggatggcagg aaaaatgtca catgtttaaa ataagagtga aaccaacgac
     7741 ctggacttat agagactgtg tgtccttagg caaagctctt gtacaaacag tgcttgtgac
     7801 tgtgaaattc ggggtttgcc tggattgtgt taaatgtgtg cttggatgta tcataaaaaa
     7861 gaatcggttc tctgtatttg acattttctg taatttatct aaagttacaa ctggctatgc
     7921 ctgattttta tcactattga atcaatcatt ttaaagaatg ttaagtctgc tgatttggag
     7981 agctcattta catttatttt gaatgtctct tatttaacac aactgattta agctttagat
     8041 ctgcgaagat acggccacgg gtgctcttga tcctgtggct gattttggac tgtgctgctc
     8101 gcagctgctg atgaatcaca tacttcctcc attttcttcc actgattgac tgttataatt
     8161 tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca tgattgcatt
     8221 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     8281 tacaaatgtg gtatggctga ttatgatcct ctagatcaga tccactagtt ctagagcggc
     8341 cattaccctg ttatccctag gccgccaccg cggtggagct ccaattcgcc ctatagtgag
     8401 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     8461 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     8521 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggaaattg
     8581 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     8641 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     8701 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     8761 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     8821 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     8881 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     8941 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     9001 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     9061 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     9121 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     9181 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     9241 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     9301 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     9361 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     9421 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     9481 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9541 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9601 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9661 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9721 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9781 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9841 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     9901 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9961 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    10021 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    10081 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    10141 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    10201 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    10261 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    10321 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    10381 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    10441 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    10501 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10561 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    10621 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10681 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10741 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10801 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    10861 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    10921 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    10981 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    11041 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    11101 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    11161 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    11221 gggaacaaaa gctgggtacc gggccccccc tcgaattacc ctgttatccc tatcgagcaa
    11281 aatcaatcct aatgtaaaat agtatacgtt catcattata atgcaaacac tgtagaactg
    11341 ttgtgttgtg ccttgcattt cattctcagt ttcttatcgt ctgtctcttt ttacgacaga
    11401 gctagaaaaa actagccttc atttgccatc gccgtatgca aatccactcc agaactcaac
    11461 aggtttgttc tgcggcgggt gtaagtgtga acaccggcat gtaagtgcgc ttgagcagga
    11521 gaataaagcg ctcagagaca ccggaggaat cctttatttt gcgttgcgcc tcaggggatt
    11581 cgctgagcct tctcttcatg gccctgctct tcagacacag gtgctccgct gtttgtgttt
    11641 ttcactgttc ttcag
//
