LOCUS       sequence-443742-       10496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of full-length TNRC6A with S1332D/S1346D mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Munakata F, Suzawa M, Ui-Tei K
  TITLE     Identification of Phosphorylated Amino Acids in Human TNRC6A 
            C-Terminal Region and Their Effects on the Interaction with the 
            CCR4-NOT Complex.
  JOURNAL   Genes (Basel). 2021 Feb 13;12(2):271. doi: 10.3390/genes12020271.
  PUBMED    33668648
REFERENCE   2  (bases 1 to 10496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2021-02-13"; volume: "12(2):271. doi"; pages: " 
            10.3390/genes12020271"
COMMENT     Sequence Label: pcDNA.5.1-FHS-TNRC6A-FL-S1332D/S1346D
FEATURES             Location/Qualifiers
     source          1..10496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..62)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        614..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     768..788
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    819..837
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    840..858
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     868..892
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      988..997
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             997..1020
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1021..1047
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1051..1164
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     protein_bind    5394..5405
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(6329..6345)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6330..6346)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6401..6420)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6402..6420)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6447..6464)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6453..6677
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6810..6829)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6961..7008
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7016..8038
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7016..8038
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8168..8289
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8205..8224)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8259..8278
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8338..8354)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8338..8354)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8351..8373)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8362..8378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8386..8392,8393..8410,8411..8416))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8431..8452
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8569..8586)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8740..9328)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8820..8839)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9499..10290,10291..10359))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10122..10141
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10360..10464)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg atagagatct
      841 ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag
      901 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccggac
      961 tctagcgttt ccaccgcggt ggcggccgcc accatggatt acaaggatga cgacgataag
     1021 tacccatacg atgttccaga ttacgcttct atggacgaga agaccaccgg ctggcggggc
     1081 ggccacgtgg tggagggcct ggccggcgag ctggagcagc tgcgggccag gctggagcac
     1141 caccctcagg gccagcggga gccctgcccg ggcggatcca tggatgctga ttctgcctcc
     1201 agttctgaat cagagagaaa catcactatc atggcttcag ggaacacagg tggtgaaaaa
     1261 gatggccttc ggaatagcac tggacttggt tcccaaaaca agtttgtagt tggtagcagc
     1321 agcaataatg tgggccatgg aagtagtact gggccatggg gtttttccca tggagccata
     1381 ataagcacat gtcaggtctc tgtggatgct cctgaaagca aatctgaaag tagcaacaat
     1441 agaatgaatg cttggggcac tgtaagttct tcatcaaatg gagggttaaa tccaagcact
     1501 ttgaattcag ctagcaacca tggtgcctgg ccagtattag agaacaatgg acttgcccta
     1561 aaagggcctg tagggagtgg tagttctggc attaatattc agtgcagtac tataggccag
     1621 atgcctaaca atcagagtat taactctaaa gtgagtggtg gttctaccca tggtacctgg
     1681 ggaagccttc aggaaacttg tgaatctgaa gtaagtggta cacagaaggt ttcattcagt
     1741 ggtcaacctc aaaatattac cactgaaatg actggaccaa ataacactac taactttatg
     1801 acctctagtt taccaaactc cggttcagtg cagaataatg agctgcctag tagtaacaca
     1861 ggggcctggc gtgtgagcac aatgaatcat cctcagatgc aggctccatc aggtatgaat
     1921 ggcacttccc tttctcacct tagcaatgga gagtcaaaaa gtggaggctc ttatggtact
     1981 acatggggtg cctatggttc taattactct ggagacaaat gttcaggccc taatggccaa
     2041 gctaatggtg acactgtgaa tgcaactcta atgcagcctg gcgtaaatgg tcctatgggc
     2101 actaactttc aagttaacac aaacaaagga ggtggtgtgt gggaatctgg tgcagcaaac
     2161 tcccagagta catcatgggg aagtggaaat ggcgcaaatt ctggaggaag tcgaagagga
     2221 tggggaaccc ctgcacaaaa cactggcact aatttaccca gcgttgagtg gaacaaactg
     2281 cctagcaatc agcattccaa tgatagtgca aatggcaatg gtaagacgtt tacaaatgga
     2341 tggaaatcta ctgaggaaga ggatcagggt tctgccacat ctcagacaaa tgagcaaagc
     2401 agtgtgtggg ccaaaacagg aggtacagtg gagagcgatg gtagtacaga aagcactgga
     2461 cgccttgagg aaaaaggaac tggggaaagt cagagtagag acagaagaaa aattgatcag
     2521 cacacattac tccaaagcat tgtaaacaga actgacttag atccacgtgt cctgtccaac
     2581 tctggttggg gacagactcc tattaagcag aatactgcct gggatacaga aacatcacct
     2641 agaggggaac gaaagactga caatgggaca gaggcctggg gaagctctgc aacacagact
     2701 tttaactcag gggcatgtat agataagact agccctaatg gtaatgatac ctcatctgta
     2761 tcagggtggg gcgatcccaa acctgctctg aggtggggag attccaaagg ctcaaactgc
     2821 cagggggggt gggaagatga ttctgctgct acaggaatgg tcaagagcaa tcagtggggg
     2881 aattgcaaag aggagaaggc tgcatggaat gactcgcaaa agaataaaca gggatggggt
     2941 gatggacaaa aatcaagcca agggtggtct gtttctgcca gtgataactg gggagaaact
     3001 tcaaggaata accattgggg tgaggccaat aagaaatcca gctcaggagg tagtgacagt
     3061 gacaggtccg tttccggttg gaacgaactt ggtaaaacta gttctttcac ttggggaaac
     3121 aacataaatc caaataattc atcaggatgg gatgaatctt ctaaacctac tccttcccag
     3181 ggatggggag accctccaaa gtctaatcag tctctaggtt ggggagattc gtcaaagcca
     3241 gtcagctctc cagactggaa caagcaacaa gacattgttg gatcttgggg aatcccacca
     3301 gctacaggca aacctcctgg tacaggctgg ctggggggac ctataccagc cccagcaaaa
     3361 gaagaagaac ccacaggctg ggaggaacca tccccagaat ctatacgtcg caaaatggag
     3421 attgatgatg gaacttcagc ttggggagat ccaagcaaat acaactacaa aaatgtgaac
     3481 atgtggaaca aaaacgtccc aaatggcaac agccgttcag accagcaagc acaggtacat
     3541 cagctgctaa cgcctgcaag tgccatctca aacaaagagg caagcagtgg ctctggctgg
     3601 ggtgagccct ggggggagcc ttctactcca gccacaactg tggataatgg tacttcagca
     3661 tggggtaagc ccatagacag tggtcccagc tggggggaac ccattgctgc ggcatccagc
     3721 acatccacgt ggggctccag ctctgttggt ccacaagcat taagcaaatc tgggccaaaa
     3781 tctatgcaag atggctggtg tggtgatgat atgccattgc ctggaaatcg ccccactggc
     3841 tgggaagagg aagaggatgt ggagattgga atgtggaata gtaattcatc tcaagagctt
     3901 aactcatctt taaattggcc accatataca aagaaaatgt catcgaaggg tctgagtggc
     3961 aaaaaaagga gaagggaaag gggaatgatg aaaggtggaa acaaacaaga agaagcgtgg
     4021 ataaatccat ttgttaaaca gttttcaaac atcagttttt cgagagactc accagaggaa
     4081 aatgtacaaa gcaataagat ggacctttct ggaggaatgt tacaagacaa acgaatggag
     4141 atagataaac atagcctaaa tattggtgat tacaatcgaa cggtcgggaa aggccctggt
     4201 tctcggcctc agatttccaa agagtcttcc atggagcgca atccttattt tgataaggat
     4261 ggcattgtag cagatgaatc ccaaaacatg cagtttatgt ccagtcaaag catgaagctt
     4321 cccccttcaa atagtgcact acctaaccag gcccttggct ccatagcagg gctgggtatg
     4381 caaaacttga attctgttag acagaatggc aatcccagta tgtttggtgt tggaaacaca
     4441 gcagcacaac cccggggcat gcagcagcct ccagcacaac ctcttagttc atctcagcct
     4501 aatctccgtg ctcaagtgcc tcctccatta ctctcccctc aggttccagt ttcattgctg
     4561 aagtatgcac caaacaacgg tggcctgaat ccactctttg gccctcaaca ggtagccatg
     4621 ctgaaccagc tatcccagct aaaccagctt tctcagatct cccagttaca gcgattgtta
     4681 gcgcagcagc aaagggcgca gagtcagaga agcgtgcctt ctgggaaccg gccgcagcaa
     4741 gaccagcagg gtcgacctct tagtgtgcag cagcaaatga tgcaacaatc tcgtcaactt
     4801 gatccaaacc tgttggtgaa gcagcagact ccaccatctc agcagcagcc actccatcag
     4861 ccagccatga agtctttcct tgacaatgtc atgccccaca ctacacctga gctgcaaaaa
     4921 gggccatcac caataaatgc tttcagcaac ttccctatag gcttgaactc aaacttgaat
     4981 gtaaatatgg atatgaacag tattaaagag ccacagtcaa gactaaggaa gtggacgaca
     5041 gtggacagca tttctgtgaa cacatctttg gatcaaaact ccagcaaaca tggtgctatt
     5101 tcaagtggtt tcaggctgga agagtctcca tttgttccct atgactttat gaacagcagt
     5161 acttcaccag ccgatcctcc aggttcaata ggagatggct ggccacgtgc caaagatcct
     5221 aacggctcta gcagtgttaa ttggccacca gaatttcgtc ctggtgagcc atggaaaggt
     5281 tatccaaaca ttgaccctga aactgaccct tacgtcactc ctggcagtgt cataaacaat
     5341 ctttcaatta atactgtgcg ggaagttgac cacctcaggg acaggaacag tgggtcatcc
     5401 tcatccttga acaccacgct gccttcaact agtgcctggt catccattcg tgcctccaac
     5461 tacaacgttc ccctcagcag tacagcacaa agcacttcag ccagaaatag tgattccaaa
     5521 ttgacatggt ctcctggttc agttacaaac acctctctgg ctcatgagct gtggaaggtc
     5581 cctttgccac ctaaaaacat cactgctccg tcccgcccac ctccgggact gactggtcag
     5641 aagccaccct tgtctacgtg ggataattct ccccttcgta taggtggagg atggggaaat
     5701 tctgacgcca gatatacccc aggttccagc tggggtgaga gcagctcagg gagaataaca
     5761 aattggcttg ttctaaaaaa ccttacacct cagatcgatg gctcaactct gcgcactctg
     5821 tgcatgcagc acggcccgct gatcacattc cacctgaacc tccctcacgg aaatgctctg
     5881 gtccgctaca gttcaaaaga agaggtagtg aaggcacaaa agtctctgca catgtgtgta
     5941 ctggggaaca ctactattct tgctgagttt gccagtgaag aggagatcag tcgtttcttt
     6001 gcacaaagcc agtctctgac cccttctccc ggctggcagt ctctcgggtc cagccagagc
     6061 cggctgggct ccctcgactg ttcccactca ttctccagcc ggaccgatct caatcactgg
     6121 aatggtgctg ggctgtcggg aactaactgt ggagaccttc acggcacttc actctggggg
     6181 accccgcatt attccacaag cctgtggggt cccccaagca gcagcgaccc ccgaggaatt
     6241 agcagcccat ctcccattaa cgcttttctt tctgttgacc acctgggtgg gggtggagag
     6301 tccatgtaat ctaggatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta
     6361 ccttaattaa ttaaggtacc aggtaagtgt acccaattcg ccctatagtg agtcgtatta
     6421 caattcactc gaaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     6481 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     6541 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     6601 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     6661 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     6721 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6781 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6841 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6901 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtaccta
     6961 gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcct tggccaaaaa
     7021 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
     7081 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
     7141 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
     7201 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt
     7261 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
     7321 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
     7381 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
     7441 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
     7501 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
     7561 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
     7621 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
     7681 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
     7741 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
     7801 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
     7861 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
     7921 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
     7981 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagca
     8041 cgtactacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     8101 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     8161 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8221 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8281 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     8341 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8401 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8461 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8521 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8581 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8641 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8701 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8761 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8821 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8881 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8941 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9001 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9061 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9121 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9181 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9241 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9301 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9361 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9421 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9481 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9541 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9601 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9661 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9721 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9781 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9841 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9901 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9961 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10021 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10081 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10141 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10201 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10261 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10321 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10381 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10441 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcg
//
