LOCUS       sequence-44376-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: pLenti-EF1a-KLF4
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(76..225)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(250..270)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgggccagga ttctcctcga cgtcaccgca tgttagcana cttcctctgc cctctccact
       61 gcctctagag ttaatcagca tgtccaggtc gaaatcatca agggcgtcac tgccgagcat
      121 gtcgaggtca aagtcatcaa gggcatccga accaagcatg tcaaggtcaa aatcatcgag
      181 ggcgtcactt cccagcatat ccagatcaaa atcgtccaat gcgtcagccc gtccggaacc
      241 gctggcctcc acctttctct tcttcttggg gctagctgac gcgcgagttt ggtccccttc
      301 atgcatgggc gagggcgcgt ccaaatccct ctccagcgag tctgcgaacg catgaaggct
      361 cgcctggtca ggtgtctgag ttgacgtagg tgaaggcttc gcgcgtttca taccgctcgc
      421 ttggagaatc ctgtcccacc gctgggaggc gggaggcagt gtgcccgagc gggcctcgag
      481 ttctgtgaca ccgactctac gaaagagctg cagcagtccg tgtctcgaca taccaaattg
      541 agtcatggcg tcatcgaacg cttgtgcggg gtgggagtga cactggaaga atccgagcac
      601 gcggaccact tgcgcgtgat ctgccactcg atgtgatgtc ctctcgggaa tccttctgtt
      661 ggtccgctta atcaatgcag gcgcgtgcgg gagccccttc ttcaccgcat ccaaggcggg
      721 tcgtccgccg aggcaggcca gcgctacaag atgat
//
