LOCUS       sequence-443760-       12569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CPSF6 gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12569)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 12569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:CPSF6_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..12569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    6742..6876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6897..7031
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(7333..8046,8047..8049,8050..8052))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7336..8046,8047..8049,8050..8052))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7378..7399)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7727..7746
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7986..8007
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9233..9249)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9233..9249)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(9304..9322)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9332..9349)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9332..9348)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9341..9363)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(9489..9944)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9626..9647)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9838..9857
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9971..10075
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10076..10144,10145..10936)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10294..10313)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11107..11695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11596..11615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11849..11866
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11983..12004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12019..12024,12025..12042,12043..12049)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12057..12073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12062..12084
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12081..12097
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12081..12097
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12116..12136
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12118..12136
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcactcgtgg ttacgggttt ttgttttgca ctcttgcaca cacatctctc ggcctccaaa
       61 acaaacacac aactgcacga gggctcgaag cccccaccga aaaacaaaaa cattatgcta
      121 atgaggaagg cacagctgtt cccttcttca ctctctctct cgttctttca acaacagtcg
      181 cctaatttct ctcgttcctt taaagagccg ccaagcagaa acagttaatg caaatataag
      241 cctgcgggaa ataaggagaa tttttacaaa atccgagctt cccctcctgc caaaatgtga
      301 tgtgtgactc acgcattcga tatttggtgt aatgagagtg aatcattctg ctccacaccg
      361 aacccgatgc cacagaccac tcagatgcac accgaggaga ctattttaac agcacattac
      421 atccagactg tcacagatgc attaccaaga gtgtgatttc agtgtcgaat gcaggtctta
      481 aaggaaaatc tgttgcgatt gcatgtgcgc taactagaca cgaccaacga atgggaacaa
      541 tctgtgaaat attatacaat cacaggcaat taaaagatgt ttaaagttca atttagtttt
      601 aattaatatg ttatataaaa ctaatctgtt ttaaatgtat atttatgatt ttagctttag
      661 ctagcctctt gaccagcagc tgaagtgtcc ggcaagtacc taaaagggag accacatggg
      721 aaagctaggt tgctcccgga agaggtgtta gtaaaccagc aggtctacct gtggtctgtg
      781 tgggtcctaa tgccccagta taaaccagca ggtcaacctg tggtctgtgt gggtcctaat
      841 gccccagtat attgatggag actctatact gctcggtgag ctccatcttt cggataagat
      901 gttaaaacaa ggtcccgact ctctgtggtg gtaaaaaatt cccaggatgt ccttcctacc
      961 ctggcatcct ggacaaattt gctcactgtc ctctgtccat catggcctcc taaccatccc
     1021 catatcataa ttggctttat cactctagct ggggccgaaa tcgcatactt ccattttatg
     1081 aggtacgcta aaatcagtat gcgagccaag taatatgtcc gaatacttcc ggcgagattc
     1141 tagagtgtgc attcgatgca cgctacgcta ttccatgatg ccccgtgaga gaattcttga
     1201 atcggagaga agcaacgcaa ctcatgcggg taggttacgt gacgatgaca tattggcgga
     1261 tgtagtacgt ccaagttcca ttcatactgc tcacattcat attttataga atgttctttt
     1321 cccacagcca agtagtaagt ttaaattcaa atacagtacc tactaagtag tagtagacgg
     1381 tttcggacgc agcccctgtc tcctctccac caatatactg gtgtgtagtg tgcggtctgg
     1441 tgcaatatgg ctgccatcgc gtcatccagg tggatgctgc acactggtgg ttgatgagga
     1501 gattttgccc aaaaaagtgt tttgagtttc cagaaaagcg cgatataaat gtaaggaatt
     1561 atcattagct tattataatg gaactttctg gataacttgt gccagagcta ggggccatta
     1621 aaactttgcc ctggggtacc tcagggagct gcaaggaagt gctatagggc taatgaaatg
     1681 tttgggggaa aaaaaaaaat tttataagta aaaataaaac taaaataatt aataatttca
     1741 ttgttgaaaa ttatagtaaa attgataaac aagataagca gcattagtat aaaaaactaa
     1801 aaacaaacgt aatttaaaat aattaaattg ttttaaataa attatacaaa ataatgttta
     1861 caacgacacg gtggtgcagt aggtagtgct gtcgccagca aagcaagaag gtcgctggtt
     1921 cgagcctcag ctggatcact tgatgtttct gtgtggagca ttcgcgtgcg tttaacccca
     1981 gattaataaa gagactaagc cgaaaagaaa ataaatgtat gaaaatgttt acgtaaaatg
     2041 taaataaatg catgattaat aaaatatatg aattttaaag attgaggtag aattgtttta
     2101 ttattcacac atttggttat ttttaatcag tgacaacttg tgatgctctt tatattgttt
     2161 attacgctac tttgaatatt cagtctcaaa ttgcatgtaa atacgacttc aaagcagaat
     2221 tagtttaatt taattgcctg taaacagtgt tgtactttga tggtgattgt ctgataataa
     2281 tatgatttca ctatttaaaa tttgaaaaaa aaaaatctga aaaatactca ccggccactt
     2341 tattaggtac acctgtctaa ctgcttgtta acgtaaattt ctaatcagcc aatcaccagg
     2401 cagcaactca gtgcatttag gcatgtagac atggtcaaga cgatctgctg cagttcaaag
     2461 cgagcatcag aatggagaag caaggtgatt taagtgactt taaacgtgac gtgtttgttg
     2521 gtgccagacg ggctggtctg agtatttcag aaactgctga tttactgggg ttttcacaca
     2581 caaacatctc tggggtttac agagaatggt ccaaaaaaag ataatatcca gtgagcggca
     2641 gttctgtggg ggcaaatgtc ttgttgatgc cagaggtcag aggagaatgg cctgactggt
     2701 tccagctgat agaaaggcaa cagtaattca aataaccact cgttagaact gaggcatgca
     2761 gaagagcacc tttgaacaca acatgtcgaa ccttgaggcg ggtgggctac agcagctcaa
     2821 gaccacatcg ggtgccactc ctgtcagcta agaacaggaa actgaggcta caattcgcac
     2881 aggctcaaca taagtagaca atagaagatt gaacattatg ttgcctggtc agatggatac
     2941 attctacctt gtatcaacag ttcaggctgg tgatggtggt gtaatggtgt gggggatata
     3001 ttcttggcac actttgggcc cattagtacc aattaaccat cgtgtcaacg ccacagccta
     3061 actgagtatt gttgctgacc atgttcattt ctttttgacc acagtgtacc catcttctga
     3121 tggctacttc ctgcaggata atgcaccatg tcataaagcg tgaatgatct cagactggtt
     3181 tcttgaacat gacaatgagt tcactgtgct cgaatgacct ccacagtcac cagatctcaa
     3241 tccaatagag cgcctttggg gtgtggtaga atggaagatt cgcatcctgg atgtgcagcc
     3301 gacaaatctg catgatgcta gcatgtcaat atggaccaaa atctctgagg aattttccag
     3361 tacctggttg aaatgcaaaa ggaagggaat ctatgctaag gcaaaaagag gtccaaccca
     3421 gtactagtgg ggtgtacata ataaagtggc cggtgagtgt atattgttaa ggatatctag
     3481 ataatccaaa taatttacag cactatttaa aaaaaaaact ttatgtcacc aagtctgcat
     3541 ttatttgatc acaattacag tcgaaaaagc aatatcggga tacattgttt ccatttaaaa
     3601 taactatatt atattttaaa tatttttgac aatgttattt ctttaatgcc aaagctaaat
     3661 tgtattcaat ctgaatttta gtgtcacatg atccttcaga aaatattaac acattgctaa
     3721 aaatgtatat tattatcaat ttggattttt tttttttttt tgtatattaa cctatgctat
     3781 tggttactat acaaaagtat tactgtgaat atatgcatat acagaaccgt atcaagcgcg
     3841 gggggtggtt gggttatgtc cccatttgga aagacctgga aacattcata atgactaaaa
     3901 ttgcacggaa aaatataata tctatgagta ttgctgatca gcagcacaaa accagaactc
     3961 catattaata aacagccaat gatgcagtga gtttgtgcat gttttcattc aaactcgtgt
     4021 gattgtgctc aaacacctgt tgtgagcagc agttgcaaac agtaaacctc aagaggagcc
     4081 agtcaacctt cacaaacact caacctctgg ctcactcact aaacaattcg cgttaatatc
     4141 taaaataaaa atttccagca ccaaacagct ctcagatttc ggagtacact gaccaagagg
     4201 taagaagtaa cgttacatta ctaggacgac tattgtaaga ctagatatgt aatatttgga
     4261 agcgtacgtg ttttaagtga catatagagt agaggaactg atggaaatac gatttgcata
     4321 atgtaaaatg atatatatta aaatataggc tacagtaggt aacgtggttt acatataacg
     4381 ttactgtgtt aatgtagtgc tcagttactc gtgttgacag ttattattag tgcttgctaa
     4441 tttgtttgtt aatgcttctc ttgcgtattt gattaccttc aggcgaatct aaaacatatc
     4501 agatgtagaa acggcagaaa aatgttgaag aaaatacgag aatagtagct tttggagcac
     4561 atagaaaagt acatcagata aagcctaccg tttaaacgca gacgccgctc tcgcactgaa
     4621 tgctgggtac tgtagtcctt atattgtatg cttaatgtgc gtctcaacgg gaaactagag
     4681 ttaggaaaac tacaatttcc tgtggaaaat gtttctgttt tcggggaaac agcgaaagaa
     4741 agatatggtt ccgcgttttt ttcttacttt gaataataaa ataagaccta actacacact
     4801 tcgcttactc ttatgctaat ttgaagcact agcattcttt gatacaatga taatcattta
     4861 aacatcacag ttatactaca aatactatgg ttaaactatg tctagtgtag tgtaacacta
     4921 tggttgccat tgttatcttt aagtttaagt attatctgtg gtaaagccat ggttggtttt
     4981 tataagaggt aataaaatta acattgtaca attaaatgac tataaattaa tgtttttttt
     5041 tatttttttt acacagctac cttcaaaaag ggatccatgg cggacggcgt ggaccacata
     5101 gacatttacg cggatgtcgg cgaagagttc aaccaggaag ctgaatatgg tgggcatgat
     5161 cagatagatt tgtatgacga tgtcatatct ccatctgcaa ataatggaga tgccccagaa
     5221 gaccgagatt acatggatac tctcccacca actgttggtg atgatgtggg taaaggagca
     5281 gcaccaaatg ttgtctatac atatactgga aagagaattg cattatatat tggaaatcta
     5341 acatggtgga caacagatga agacttaact gaagcagttc attctttggg agtaaatgat
     5401 attttggaga taaaattttt tgaaaatcgg gcaaatggcc agtcaaaggg gtttgccctt
     5461 gttggtgttg gatctgaagc atcttcaaaa aagttaatgg atctgttacc taaaagagaa
     5521 cttcatggtc agaatcctgt tgtaactcca tgcaataaac agttcctgag tcaatttgaa
     5581 atgcagtcca ggaaaactac acaatcagga caaatgtctg gggaaggtaa agctggtcct
     5641 ccaggaggca gttcccgtgc agcatttcca caaggtggta gaggacgggg ccgttttcca
     5701 ggggctgttc ctggtgggga cagatttcct gggccagcag gaccaggagg gccaccccca
     5761 ccttttccag ctggacagac tccaccacgt ccacccttag gtcctccagg cccacctggt
     5821 ccaccaggtc ctccacctcc tggtcaggtt ctgcctcctc ctctagctgg gcctcctaat
     5881 cgaggagatc gccctccacc accagttctt tttcctggac aaccttttgg gcagcctcca
     5941 ttgggtccac ttcctcctgg ccctccacct ccagttccag gctacggccc ccctcctggc
     6001 ccaccacctc cacaacaggg accacctcca cctccaggcc cctttccacc tcgtccaccc
     6061 ggtccacttg ggccacccct tacactagct cctcctccgc atcttcctgg accacctcca
     6121 ggtgccccac cgccagctcc gcatgtgaac ccagctttct ttcctccacc aactaacagt
     6181 ggcatgccta catcagatag ccgaggtcca ccaccaacag atccatatgg gcgacctcca
     6241 ccatatgata ggggtgacta tggcccccct ggaagggaaa tggatactgc aagaacgcca
     6301 ttgagtgaag ctgaatttga agaaatcatg aatagaaata gggcaatctc aagcagtgct
     6361 atttcgagag ctgtgtctga tgccagtgct ggtgattatg ggagtgctat tgagacactg
     6421 gtaactgcaa tttctttaat taaacaatcc aaagtatctg ctgatgatcg ttgcaaagtt
     6481 cttattagtt ctttgcaaga ttgccttcat ggaattgagt ccaagtctta tggttctgga
     6541 tcaagacgtg aacgatcaag agagagggac catagtagat cacgagaaaa gagtcgacgt
     6601 cataaatccc gtagtagaga ccgtcatgac gattattaca gagagagaag cagagaacga
     6661 gagaggcacc gggatcgtga ccgagaccgt gaccgagagc gtgaccgaga gcgcgaatat
     6721 cgtcatcgtt agatcgatga tgatccagac atgataagat acattgatga gtttggacaa
     6781 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     6841 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattc gatgatgatc
     6901 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     6961 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     7021 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     7081 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc
     7141 tagatcagat ctcttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7201 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7261 caatgtatct tatcatgtct ggatctacgt aatacgactc actatagttc tagaggctcg
     7321 agaggggccg ctttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
     7381 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
     7441 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
     7501 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
     7561 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
     7621 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
     7681 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
     7741 gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
     7801 gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag
     7861 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag
     7921 ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc
     7981 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcttcgcc
     8041 cttgctcacc atggtcactg tctgctttgc tgttggtctg ggctcctggg tcactggctt
     8101 actaatggag tctttatgta tgaggactct tatcaattgt tcttctataa aggtctgcag
     8161 tgtttctgtt cgtcccctac atggacaccc agagcctcct aaatacagga gccctgataa
     8221 ctgcacaagt gctcagattc cagcagggtg gaaatgagat aaagtgtgca gatggggagg
     8281 gggacgtgaa tgagagattt gagggatgaa aaggatggat gaacgcattg aaaatagccc
     8341 ctttcacaaa ataataccag taaattgtca tataattaat gaccagacct ttacctgtaa
     8401 atgatgtgat gttcatatac agaagaatgc caacaattta cagatgattt tacaaattct
     8461 taattttcct tttctaaatg gatttgttca cacattatct cttcaaagca attgaatttt
     8521 ctggaaaaga ttggctgtgt gtgtaaaagg tgctactgag gaatttaatg tgacatggaa
     8581 tgaagcaaac agcagcctaa gacaggatgg caggaaaaat gtcacatgtt taaaataaga
     8641 gtgaaaccaa cgacctggac ttatagagac tgtgtgtcct taggcaaagc tcttgtacaa
     8701 acagtgcttg tgactgtgaa attcggggtt tgcctggatt gtgttaaatg tgtgcttgga
     8761 tgtatcataa aaaagaatcg gttctctgta tttgacattt tctgtaattt atctaaagtt
     8821 acaactggct atgcctgatt tttatcacta ttgaatcaat cattttaaag aatgttaagt
     8881 ctgctgattt ggagagctca tttacattta ttttgaatgt ctcttattta acacaactga
     8941 tttaagcttt agatctgcga agatacggcc acgggtgctc ttgatcctgt ggctgatttt
     9001 ggactgtgct gctcgcagct gctgatgaat cacatacttc ctccattttc ttccactgat
     9061 tgactgttat aatttcccta atttccaggt caaggtgctg tgcattgtgg taatagatgt
     9121 gacatgattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     9181 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagat cagatccact
     9241 agttctagag cggccattac cctgttatcc ctaggccgcc accgcggtgg agctccaatt
     9301 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     9361 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     9421 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     9481 gcgaatggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     9541 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     9601 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     9661 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     9721 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     9781 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     9841 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     9901 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     9961 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    10021 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
    10081 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    10141 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    10201 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    10261 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
    10321 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    10381 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    10441 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    10501 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    10561 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    10621 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    10681 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    10741 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    10801 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    10861 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    10921 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    10981 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    11041 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    11101 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    11161 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    11221 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
    11281 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    11341 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    11401 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    11461 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
    11521 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    11581 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    11641 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    11701 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
    11761 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
    11821 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
    11881 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
    11941 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    12001 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
    12061 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
    12121 taaccctcac taaagggaac aaaagctggg taccgggccc cccctcgaat taccctgtta
    12181 tccctatcga gcaaaatcaa tcctaatgta aaatagtata cgttcatcat tataatgcaa
    12241 acactgtaga actgttgtgt tgtgccttgc atttcattct cagtttctta tcgtctgtct
    12301 ctttttacga cagagctaga aaaaactagc cttcatttgc catcgccgta tgcaaatcca
    12361 ctccagaact caacaggttt gttctgcggc gggtgtaagt gtgaacaccg gcatgtaagt
    12421 gcgcttgagc aggagaataa agcgctcaga gacaccggag gaatccttta ttttgcgttg
    12481 cgcctcaggg gattcgctga gccttctctt catggccctg ctcttcagac acaggtgctc
    12541 cgctgtttgt gtttttcact gttcttcag
//
