LOCUS       sequence-443763-       11919 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express TSFM gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11919)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 11919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:TSFM_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..11919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    6092..6226
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6247..6381
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(6683..7396,7397..7399,7400..7402))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6686..7396,7397..7399,7400..7402))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6728..6749)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7077..7096
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7336..7357
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8583..8599)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8583..8599)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(8654..8672)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8682..8699)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8682..8698)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8691..8713)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(8839..9294)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8976..8997)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9188..9207
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9321..9425
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9426..9494,9495..10286)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9644..9663)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10457..11045
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10946..10965
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11199..11216
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11333..11354
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11369..11374,11375..11392,11393..11399)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11407..11423
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11412..11434
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11431..11447
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11431..11447
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11466..11486
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11468..11486
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcactcgtgg ttacgggttt ttgttttgca ctcttgcaca cacatctctc ggcctccaaa
       61 acaaacacac aactgcacga gggctcgaag cccccaccga aaaacaaaaa cattatgcta
      121 atgaggaagg cacagctgtt cccttcttca ctctctctct cgttctttca acaacagtcg
      181 cctaatttct ctcgttcctt taaagagccg ccaagcagaa acagttaatg caaatataag
      241 cctgcgggaa ataaggagaa tttttacaaa atccgagctt cccctcctgc caaaatgtga
      301 tgtgtgactc acgcattcga tatttggtgt aatgagagtg aatcattctg ctccacaccg
      361 aacccgatgc cacagaccac tcagatgcac accgaggaga ctattttaac agcacattac
      421 atccagactg tcacagatgc attaccaaga gtgtgatttc agtgtcgaat gcaggtctta
      481 aaggaaaatc tgttgcgatt gcatgtgcgc taactagaca cgaccaacga atgggaacaa
      541 tctgtgaaat attatacaat cacaggcaat taaaagatgt ttaaagttca atttagtttt
      601 aattaatatg ttatataaaa ctaatctgtt ttaaatgtat atttatgatt ttagctttag
      661 ctagcctctt gaccagcagc tgaagtgtcc ggcaagtacc taaaagggag accacatggg
      721 aaagctaggt tgctcccgga agaggtgtta gtaaaccagc aggtctacct gtggtctgtg
      781 tgggtcctaa tgccccagta taaaccagca ggtcaacctg tggtctgtgt gggtcctaat
      841 gccccagtat attgatggag actctatact gctcggtgag ctccatcttt cggataagat
      901 gttaaaacaa ggtcccgact ctctgtggtg gtaaaaaatt cccaggatgt ccttcctacc
      961 ctggcatcct ggacaaattt gctcactgtc ctctgtccat catggcctcc taaccatccc
     1021 catatcataa ttggctttat cactctagct ggggccgaaa tcgcatactt ccattttatg
     1081 aggtacgcta aaatcagtat gcgagccaag taatatgtcc gaatacttcc ggcgagattc
     1141 tagagtgtgc attcgatgca cgctacgcta ttccatgatg ccccgtgaga gaattcttga
     1201 atcggagaga agcaacgcaa ctcatgcggg taggttacgt gacgatgaca tattggcgga
     1261 tgtagtacgt ccaagttcca ttcatactgc tcacattcat attttataga atgttctttt
     1321 cccacagcca agtagtaagt ttaaattcaa atacagtacc tactaagtag tagtagacgg
     1381 tttcggacgc agcccctgtc tcctctccac caatatactg gtgtgtagtg tgcggtctgg
     1441 tgcaatatgg ctgccatcgc gtcatccagg tggatgctgc acactggtgg ttgatgagga
     1501 gattttgccc aaaaaagtgt tttgagtttc cagaaaagcg cgatataaat gtaaggaatt
     1561 atcattagct tattataatg gaactttctg gataacttgt gccagagcta ggggccatta
     1621 aaactttgcc ctggggtacc tcagggagct gcaaggaagt gctatagggc taatgaaatg
     1681 tttgggggaa aaaaaaaaat tttataagta aaaataaaac taaaataatt aataatttca
     1741 ttgttgaaaa ttatagtaaa attgataaac aagataagca gcattagtat aaaaaactaa
     1801 aaacaaacgt aatttaaaat aattaaattg ttttaaataa attatacaaa ataatgttta
     1861 caacgacacg gtggtgcagt aggtagtgct gtcgccagca aagcaagaag gtcgctggtt
     1921 cgagcctcag ctggatcact tgatgtttct gtgtggagca ttcgcgtgcg tttaacccca
     1981 gattaataaa gagactaagc cgaaaagaaa ataaatgtat gaaaatgttt acgtaaaatg
     2041 taaataaatg catgattaat aaaatatatg aattttaaag attgaggtag aattgtttta
     2101 ttattcacac atttggttat ttttaatcag tgacaacttg tgatgctctt tatattgttt
     2161 attacgctac tttgaatatt cagtctcaaa ttgcatgtaa atacgacttc aaagcagaat
     2221 tagtttaatt taattgcctg taaacagtgt tgtactttga tggtgattgt ctgataataa
     2281 tatgatttca ctatttaaaa tttgaaaaaa aaaaatctga aaaatactca ccggccactt
     2341 tattaggtac acctgtctaa ctgcttgtta acgtaaattt ctaatcagcc aatcaccagg
     2401 cagcaactca gtgcatttag gcatgtagac atggtcaaga cgatctgctg cagttcaaag
     2461 cgagcatcag aatggagaag caaggtgatt taagtgactt taaacgtgac gtgtttgttg
     2521 gtgccagacg ggctggtctg agtatttcag aaactgctga tttactgggg ttttcacaca
     2581 caaacatctc tggggtttac agagaatggt ccaaaaaaag ataatatcca gtgagcggca
     2641 gttctgtggg ggcaaatgtc ttgttgatgc cagaggtcag aggagaatgg cctgactggt
     2701 tccagctgat agaaaggcaa cagtaattca aataaccact cgttagaact gaggcatgca
     2761 gaagagcacc tttgaacaca acatgtcgaa ccttgaggcg ggtgggctac agcagctcaa
     2821 gaccacatcg ggtgccactc ctgtcagcta agaacaggaa actgaggcta caattcgcac
     2881 aggctcaaca taagtagaca atagaagatt gaacattatg ttgcctggtc agatggatac
     2941 attctacctt gtatcaacag ttcaggctgg tgatggtggt gtaatggtgt gggggatata
     3001 ttcttggcac actttgggcc cattagtacc aattaaccat cgtgtcaacg ccacagccta
     3061 actgagtatt gttgctgacc atgttcattt ctttttgacc acagtgtacc catcttctga
     3121 tggctacttc ctgcaggata atgcaccatg tcataaagcg tgaatgatct cagactggtt
     3181 tcttgaacat gacaatgagt tcactgtgct cgaatgacct ccacagtcac cagatctcaa
     3241 tccaatagag cgcctttggg gtgtggtaga atggaagatt cgcatcctgg atgtgcagcc
     3301 gacaaatctg catgatgcta gcatgtcaat atggaccaaa atctctgagg aattttccag
     3361 tacctggttg aaatgcaaaa ggaagggaat ctatgctaag gcaaaaagag gtccaaccca
     3421 gtactagtgg ggtgtacata ataaagtggc cggtgagtgt atattgttaa ggatatctag
     3481 ataatccaaa taatttacag cactatttaa aaaaaaaact ttatgtcacc aagtctgcat
     3541 ttatttgatc acaattacag tcgaaaaagc aatatcggga tacattgttt ccatttaaaa
     3601 taactatatt atattttaaa tatttttgac aatgttattt ctttaatgcc aaagctaaat
     3661 tgtattcaat ctgaatttta gtgtcacatg atccttcaga aaatattaac acattgctaa
     3721 aaatgtatat tattatcaat ttggattttt tttttttttt tgtatattaa cctatgctat
     3781 tggttactat acaaaagtat tactgtgaat atatgcatat acagaaccgt atcaagcgcg
     3841 gggggtggtt gggttatgtc cccatttgga aagacctgga aacattcata atgactaaaa
     3901 ttgcacggaa aaatataata tctatgagta ttgctgatca gcagcacaaa accagaactc
     3961 catattaata aacagccaat gatgcagtga gtttgtgcat gttttcattc aaactcgtgt
     4021 gattgtgctc aaacacctgt tgtgagcagc agttgcaaac agtaaacctc aagaggagcc
     4081 agtcaacctt cacaaacact caacctctgg ctcactcact aaacaattcg cgttaatatc
     4141 taaaataaaa atttccagca ccaaacagct ctcagatttc ggagtacact gaccaagagg
     4201 taagaagtaa cgttacatta ctaggacgac tattgtaaga ctagatatgt aatatttgga
     4261 agcgtacgtg ttttaagtga catatagagt agaggaactg atggaaatac gatttgcata
     4321 atgtaaaatg atatatatta aaatataggc tacagtaggt aacgtggttt acatataacg
     4381 ttactgtgtt aatgtagtgc tcagttactc gtgttgacag ttattattag tgcttgctaa
     4441 tttgtttgtt aatgcttctc ttgcgtattt gattaccttc aggcgaatct aaaacatatc
     4501 agatgtagaa acggcagaaa aatgttgaag aaaatacgag aatagtagct tttggagcac
     4561 atagaaaagt acatcagata aagcctaccg tttaaacgca gacgccgctc tcgcactgaa
     4621 tgctgggtac tgtagtcctt atattgtatg cttaatgtgc gtctcaacgg gaaactagag
     4681 ttaggaaaac tacaatttcc tgtggaaaat gtttctgttt tcggggaaac agcgaaagaa
     4741 agatatggtt ccgcgttttt ttcttacttt gaataataaa ataagaccta actacacact
     4801 tcgcttactc ttatgctaat ttgaagcact agcattcttt gatacaatga taatcattta
     4861 aacatcacag ttatactaca aatactatgg ttaaactatg tctagtgtag tgtaacacta
     4921 tggttgccat tgttatcttt aagtttaagt attatctgtg gtaaagccat ggttggtttt
     4981 tataagaggt aataaaatta acattgtaca attaaatgac tataaattaa tgtttttttt
     5041 tatttttttt acacagctac cttcaaaaag ggatcaatcg cggctagaga gatgtcgctg
     5101 ctgcggtcgc tgcgcgtgtt tctggtcgcg cggaccggga gctacccggc tgggtctctt
     5161 ctgcgtcagt cgccccagcc aaggcacaca ttttatgctg ggccccgtct gtctgcctcg
     5221 gcctccagca aggagctcct catgaagctg cggcggaaaa caggctactc ctttgtaaat
     5281 tgcaagaaag ctctggagac ttgtggcggg gacctcaaac aggcagagat ctggctccac
     5341 aaggaggccc agaaggaggg ctggagcaaa gctgccaagc tccaagggag gaagaccaaa
     5401 gaaggcctga ttgggctgtt gcaggaagga aacacaactg tattagtaga ggtaaactgt
     5461 gagacagatt ttgtttctag aaatttaaaa tttcaactgt tggtccagca agtagccctt
     5521 ggaaccatga tgcattgtca gaccctaaag gatcaaccct ctgcatacag taaaggtttc
     5581 ttgaattcct ctgagctttc tggacttcca gctgggcctg acagagaagg ctcactcaag
     5641 gatcagttgg ctttagcaat tggaaaactg ggagaaaaca tgattcttaa acgagctgca
     5701 tgggtgaagg tgccatctgg gttctacgtt ggctcttatg tccacggagc aatgcagagt
     5761 ccctcacttc acaagctggt gctggggaag tatggggccc tggtcatctg tgagacgtct
     5821 gaacagaaaa caaaccttga agacgttggc cgccgccttg ggcagcatgt ggtgggcatg
     5881 gcccccctct ctgttggctc cctggacgat gagcctgggg gagaggcaga gactaagatg
     5941 ctgtcccagc cgtatttgct ggatccctcc attaccttgg ggcagtatgt gcagcctcag
     6001 ggggtgtcgg tagtagactt tgtgcggttt gaatgtggag aaggtgaaga ggcagcagaa
     6061 actgaatagg ttccagagac ttatcgatga tgatccagac atgataagat acattgatga
     6121 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     6181 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattc
     6241 gatgatgatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     6301 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     6361 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     6421 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     6481 tatgatcctc tagatcagat ctcttgttta ttgcagctta taatggttac aaataaagca
     6541 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6601 ccaaactcat caatgtatct tatcatgtct ggatctacgt aatacgactc actatagttc
     6661 tagaggctcg agaggggccg ctttacttgt acagctcgtc catgccgaga gtgatcccgg
     6721 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
     6781 gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
     6841 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
     6901 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
     6961 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
     7021 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
     7081 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
     7141 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
     7201 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
     7261 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
     7321 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
     7381 gctcttcgcc cttgctcacc atggtcactg tctgctttgc tgttggtctg ggctcctggg
     7441 tcactggctt actaatggag tctttatgta tgaggactct tatcaattgt tcttctataa
     7501 aggtctgcag tgtttctgtt cgtcccctac atggacaccc agagcctcct aaatacagga
     7561 gccctgataa ctgcacaagt gctcagattc cagcagggtg gaaatgagat aaagtgtgca
     7621 gatggggagg gggacgtgaa tgagagattt gagggatgaa aaggatggat gaacgcattg
     7681 aaaatagccc ctttcacaaa ataataccag taaattgtca tataattaat gaccagacct
     7741 ttacctgtaa atgatgtgat gttcatatac agaagaatgc caacaattta cagatgattt
     7801 tacaaattct taattttcct tttctaaatg gatttgttca cacattatct cttcaaagca
     7861 attgaatttt ctggaaaaga ttggctgtgt gtgtaaaagg tgctactgag gaatttaatg
     7921 tgacatggaa tgaagcaaac agcagcctaa gacaggatgg caggaaaaat gtcacatgtt
     7981 taaaataaga gtgaaaccaa cgacctggac ttatagagac tgtgtgtcct taggcaaagc
     8041 tcttgtacaa acagtgcttg tgactgtgaa attcggggtt tgcctggatt gtgttaaatg
     8101 tgtgcttgga tgtatcataa aaaagaatcg gttctctgta tttgacattt tctgtaattt
     8161 atctaaagtt acaactggct atgcctgatt tttatcacta ttgaatcaat cattttaaag
     8221 aatgttaagt ctgctgattt ggagagctca tttacattta ttttgaatgt ctcttattta
     8281 acacaactga tttaagcttt agatctgcga agatacggcc acgggtgctc ttgatcctgt
     8341 ggctgatttt ggactgtgct gctcgcagct gctgatgaat cacatacttc ctccattttc
     8401 ttccactgat tgactgttat aatttcccta atttccaggt caaggtgctg tgcattgtgg
     8461 taatagatgt gacatgattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     8521 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagat
     8581 cagatccact agttctagag cggccattac cctgttatcc ctaggccgcc accgcggtgg
     8641 agctccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     8701 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     8761 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     8821 agcctgaatg gcgaatggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     8881 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     8941 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     9001 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     9061 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     9121 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     9181 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     9241 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     9301 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     9361 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     9421 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     9481 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     9541 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9601 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     9661 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     9721 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     9781 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9841 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9901 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9961 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    10021 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    10081 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    10141 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    10201 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    10261 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    10321 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    10381 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    10441 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    10501 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    10561 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    10621 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    10681 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    10741 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    10801 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    10861 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    10921 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10981 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    11041 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    11101 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    11161 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    11221 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    11281 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    11341 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    11401 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    11461 agcgcgcaat taaccctcac taaagggaac aaaagctggg taccgggccc cccctcgaat
    11521 taccctgtta tccctatcga gcaaaatcaa tcctaatgta aaatagtata cgttcatcat
    11581 tataatgcaa acactgtaga actgttgtgt tgtgccttgc atttcattct cagtttctta
    11641 tcgtctgtct ctttttacga cagagctaga aaaaactagc cttcatttgc catcgccgta
    11701 tgcaaatcca ctccagaact caacaggttt gttctgcggc gggtgtaagt gtgaacaccg
    11761 gcatgtaagt gcgcttgagc aggagaataa agcgctcaga gacaccggag gaatccttta
    11821 ttttgcgttg cgcctcaggg gattcgctga gccttctctt catggccctg ctcttcagac
    11881 acaggtgctc cgctgtttgt gtttttcact gttcttcag
//
