LOCUS       sequence-443764-       12163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express WDR4 gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 12163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:WDR4_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..12163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    6336..6470
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6491..6625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(6927..7640,7641..7643,7644..7646))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6930..7640,7641..7643,7644..7646))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6972..6993)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7321..7340
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7580..7601
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8827..8843)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8827..8843)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(8898..8916)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8926..8943)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8926..8942)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8935..8957)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(9083..9538)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9220..9241)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9432..9451
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9565..9669
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9670..9738,9739..10530)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10701..11289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11190..11209
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11443..11460
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11577..11598
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11613..11618,11619..11636,11637..11643)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11651..11667
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11656..11678
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11675..11691
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11675..11691
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11710..11730
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11712..11730
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcactcgtgg ttacgggttt ttgttttgca ctcttgcaca cacatctctc ggcctccaaa
       61 acaaacacac aactgcacga gggctcgaag cccccaccga aaaacaaaaa cattatgcta
      121 atgaggaagg cacagctgtt cccttcttca ctctctctct cgttctttca acaacagtcg
      181 cctaatttct ctcgttcctt taaagagccg ccaagcagaa acagttaatg caaatataag
      241 cctgcgggaa ataaggagaa tttttacaaa atccgagctt cccctcctgc caaaatgtga
      301 tgtgtgactc acgcattcga tatttggtgt aatgagagtg aatcattctg ctccacaccg
      361 aacccgatgc cacagaccac tcagatgcac accgaggaga ctattttaac agcacattac
      421 atccagactg tcacagatgc attaccaaga gtgtgatttc agtgtcgaat gcaggtctta
      481 aaggaaaatc tgttgcgatt gcatgtgcgc taactagaca cgaccaacga atgggaacaa
      541 tctgtgaaat attatacaat cacaggcaat taaaagatgt ttaaagttca atttagtttt
      601 aattaatatg ttatataaaa ctaatctgtt ttaaatgtat atttatgatt ttagctttag
      661 ctagcctctt gaccagcagc tgaagtgtcc ggcaagtacc taaaagggag accacatggg
      721 aaagctaggt tgctcccgga agaggtgtta gtaaaccagc aggtctacct gtggtctgtg
      781 tgggtcctaa tgccccagta taaaccagca ggtcaacctg tggtctgtgt gggtcctaat
      841 gccccagtat attgatggag actctatact gctcggtgag ctccatcttt cggataagat
      901 gttaaaacaa ggtcccgact ctctgtggtg gtaaaaaatt cccaggatgt ccttcctacc
      961 ctggcatcct ggacaaattt gctcactgtc ctctgtccat catggcctcc taaccatccc
     1021 catatcataa ttggctttat cactctagct ggggccgaaa tcgcatactt ccattttatg
     1081 aggtacgcta aaatcagtat gcgagccaag taatatgtcc gaatacttcc ggcgagattc
     1141 tagagtgtgc attcgatgca cgctacgcta ttccatgatg ccccgtgaga gaattcttga
     1201 atcggagaga agcaacgcaa ctcatgcggg taggttacgt gacgatgaca tattggcgga
     1261 tgtagtacgt ccaagttcca ttcatactgc tcacattcat attttataga atgttctttt
     1321 cccacagcca agtagtaagt ttaaattcaa atacagtacc tactaagtag tagtagacgg
     1381 tttcggacgc agcccctgtc tcctctccac caatatactg gtgtgtagtg tgcggtctgg
     1441 tgcaatatgg ctgccatcgc gtcatccagg tggatgctgc acactggtgg ttgatgagga
     1501 gattttgccc aaaaaagtgt tttgagtttc cagaaaagcg cgatataaat gtaaggaatt
     1561 atcattagct tattataatg gaactttctg gataacttgt gccagagcta ggggccatta
     1621 aaactttgcc ctggggtacc tcagggagct gcaaggaagt gctatagggc taatgaaatg
     1681 tttgggggaa aaaaaaaaat tttataagta aaaataaaac taaaataatt aataatttca
     1741 ttgttgaaaa ttatagtaaa attgataaac aagataagca gcattagtat aaaaaactaa
     1801 aaacaaacgt aatttaaaat aattaaattg ttttaaataa attatacaaa ataatgttta
     1861 caacgacacg gtggtgcagt aggtagtgct gtcgccagca aagcaagaag gtcgctggtt
     1921 cgagcctcag ctggatcact tgatgtttct gtgtggagca ttcgcgtgcg tttaacccca
     1981 gattaataaa gagactaagc cgaaaagaaa ataaatgtat gaaaatgttt acgtaaaatg
     2041 taaataaatg catgattaat aaaatatatg aattttaaag attgaggtag aattgtttta
     2101 ttattcacac atttggttat ttttaatcag tgacaacttg tgatgctctt tatattgttt
     2161 attacgctac tttgaatatt cagtctcaaa ttgcatgtaa atacgacttc aaagcagaat
     2221 tagtttaatt taattgcctg taaacagtgt tgtactttga tggtgattgt ctgataataa
     2281 tatgatttca ctatttaaaa tttgaaaaaa aaaaatctga aaaatactca ccggccactt
     2341 tattaggtac acctgtctaa ctgcttgtta acgtaaattt ctaatcagcc aatcaccagg
     2401 cagcaactca gtgcatttag gcatgtagac atggtcaaga cgatctgctg cagttcaaag
     2461 cgagcatcag aatggagaag caaggtgatt taagtgactt taaacgtgac gtgtttgttg
     2521 gtgccagacg ggctggtctg agtatttcag aaactgctga tttactgggg ttttcacaca
     2581 caaacatctc tggggtttac agagaatggt ccaaaaaaag ataatatcca gtgagcggca
     2641 gttctgtggg ggcaaatgtc ttgttgatgc cagaggtcag aggagaatgg cctgactggt
     2701 tccagctgat agaaaggcaa cagtaattca aataaccact cgttagaact gaggcatgca
     2761 gaagagcacc tttgaacaca acatgtcgaa ccttgaggcg ggtgggctac agcagctcaa
     2821 gaccacatcg ggtgccactc ctgtcagcta agaacaggaa actgaggcta caattcgcac
     2881 aggctcaaca taagtagaca atagaagatt gaacattatg ttgcctggtc agatggatac
     2941 attctacctt gtatcaacag ttcaggctgg tgatggtggt gtaatggtgt gggggatata
     3001 ttcttggcac actttgggcc cattagtacc aattaaccat cgtgtcaacg ccacagccta
     3061 actgagtatt gttgctgacc atgttcattt ctttttgacc acagtgtacc catcttctga
     3121 tggctacttc ctgcaggata atgcaccatg tcataaagcg tgaatgatct cagactggtt
     3181 tcttgaacat gacaatgagt tcactgtgct cgaatgacct ccacagtcac cagatctcaa
     3241 tccaatagag cgcctttggg gtgtggtaga atggaagatt cgcatcctgg atgtgcagcc
     3301 gacaaatctg catgatgcta gcatgtcaat atggaccaaa atctctgagg aattttccag
     3361 tacctggttg aaatgcaaaa ggaagggaat ctatgctaag gcaaaaagag gtccaaccca
     3421 gtactagtgg ggtgtacata ataaagtggc cggtgagtgt atattgttaa ggatatctag
     3481 ataatccaaa taatttacag cactatttaa aaaaaaaact ttatgtcacc aagtctgcat
     3541 ttatttgatc acaattacag tcgaaaaagc aatatcggga tacattgttt ccatttaaaa
     3601 taactatatt atattttaaa tatttttgac aatgttattt ctttaatgcc aaagctaaat
     3661 tgtattcaat ctgaatttta gtgtcacatg atccttcaga aaatattaac acattgctaa
     3721 aaatgtatat tattatcaat ttggattttt tttttttttt tgtatattaa cctatgctat
     3781 tggttactat acaaaagtat tactgtgaat atatgcatat acagaaccgt atcaagcgcg
     3841 gggggtggtt gggttatgtc cccatttgga aagacctgga aacattcata atgactaaaa
     3901 ttgcacggaa aaatataata tctatgagta ttgctgatca gcagcacaaa accagaactc
     3961 catattaata aacagccaat gatgcagtga gtttgtgcat gttttcattc aaactcgtgt
     4021 gattgtgctc aaacacctgt tgtgagcagc agttgcaaac agtaaacctc aagaggagcc
     4081 agtcaacctt cacaaacact caacctctgg ctcactcact aaacaattcg cgttaatatc
     4141 taaaataaaa atttccagca ccaaacagct ctcagatttc ggagtacact gaccaagagg
     4201 taagaagtaa cgttacatta ctaggacgac tattgtaaga ctagatatgt aatatttgga
     4261 agcgtacgtg ttttaagtga catatagagt agaggaactg atggaaatac gatttgcata
     4321 atgtaaaatg atatatatta aaatataggc tacagtaggt aacgtggttt acatataacg
     4381 ttactgtgtt aatgtagtgc tcagttactc gtgttgacag ttattattag tgcttgctaa
     4441 tttgtttgtt aatgcttctc ttgcgtattt gattaccttc aggcgaatct aaaacatatc
     4501 agatgtagaa acggcagaaa aatgttgaag aaaatacgag aatagtagct tttggagcac
     4561 atagaaaagt acatcagata aagcctaccg tttaaacgca gacgccgctc tcgcactgaa
     4621 tgctgggtac tgtagtcctt atattgtatg cttaatgtgc gtctcaacgg gaaactagag
     4681 ttaggaaaac tacaatttcc tgtggaaaat gtttctgttt tcggggaaac agcgaaagaa
     4741 agatatggtt ccgcgttttt ttcttacttt gaataataaa ataagaccta actacacact
     4801 tcgcttactc ttatgctaat ttgaagcact agcattcttt gatacaatga taatcattta
     4861 aacatcacag ttatactaca aatactatgg ttaaactatg tctagtgtag tgtaacacta
     4921 tggttgccat tgttatcttt aagtttaagt attatctgtg gtaaagccat ggttggtttt
     4981 tataagaggt aataaaatta acattgtaca attaaatgac tataaattaa tgtttttttt
     5041 tatttttttt acacagctac cttcaaaaag ggatccatgg cgggctctgt gggactggcg
     5101 ttgtgcgggc agacgttggt ggtgcggggc ggcagccgat tcctggccac ctccatagca
     5161 agcagtgatg atgacagcct cttcatctat gactgcagtg ctgcagaaaa gaagtcacaa
     5221 gaaaataaag gggaggacgc gcccttggac caggggagcg gtgcgattct ggcgtccacc
     5281 ttctccaagt ctggcagcta ttttgcttta accgatgaca gtaagcgtct gattcttttc
     5341 cgtacaaaac catggcaatg tctgagtgtc aggaccgtgg caaggaggtg tacagccctg
     5401 actttcatag cctcggagga gaaggtcttg gtggccgaca agtctggaga cgtctactcc
     5461 ttttcggtgc tggagccaca cgggtgtggc cgtctagagc tggggcacct gtctatgctg
     5521 ttagatgtgg ctgtgagtcc tgatgaccgc ttcatcctca ctgccgaccg ggacgagaag
     5581 atccgagtca gctgggccgc ggcgccccat agcatcgagt ccttctgctt ggggcacaca
     5641 gagtttgtga gccgtatctc cgtggtgcca actcagcccg ggctgcttct gtcctcctct
     5701 ggggacggca ccctgaggct ctgggagtac aggagcggcc gccagctgca ctgctgtcac
     5761 ctggccagtc tgcaggagct ggtggacccc caggcccccc agaagtttgc cgcgtccagg
     5821 attgcattct ggtgccagga gaactgcgtg gcgctcctgt gcgacggcac tcctgtggtc
     5881 tacatcttcc agctggacgc ccgcagacag cagttggtgt acaggcagca gctggcgttc
     5941 cagcaccaag tgtgggacgt ggctttcgag gagacccagg ggctgtgggt gctccaggac
     6001 tgccaggaag cccccctggt gctctacagg cctgtgggcg accagtggca gtctgttcct
     6061 gagagcaccg tgttaaagaa agtctctggt gttcttcgtg ggaactgggc catgctggaa
     6121 ggctctgccg gcgcagacgc cagcttcagc agtctctaca aggccacgtt cgacaacgtg
     6181 acctcctacc tgaagaagaa agaggagaga ctgcagcagc agctagagaa gaagcagcgg
     6241 cgccggagtc ccccgcctgg gcccgacggg catgccaaga agatgagacc gggggaggcg
     6301 acgctaagtt gctgagtcga ccctgtatcg atgatgatcc agacatgata agatacattg
     6361 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     6421 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     6481 attcgatgat gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     6541 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     6601 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     6661 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     6721 tgattatgat cctctagatc agatctcttg tttattgcag cttataatgg ttacaaataa
     6781 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6841 ttgtccaaac tcatcaatgt atcttatcat gtctggatct acgtaatacg actcactata
     6901 gttctagagg ctcgagaggg gccgctttac ttgtacagct cgtccatgcc gagagtgatc
     6961 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
     7021 ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
     7081 atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg
     7141 cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg
     7201 tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
     7261 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
     7321 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc
     7381 atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc
     7441 acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg
     7501 cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg
     7561 ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg
     7621 aacagctctt cgcccttgct caccatggtc actgtctgct ttgctgttgg tctgggctcc
     7681 tgggtcactg gcttactaat ggagtcttta tgtatgagga ctcttatcaa ttgttcttct
     7741 ataaaggtct gcagtgtttc tgttcgtccc ctacatggac acccagagcc tcctaaatac
     7801 aggagccctg ataactgcac aagtgctcag attccagcag ggtggaaatg agataaagtg
     7861 tgcagatggg gagggggacg tgaatgagag atttgaggga tgaaaaggat ggatgaacgc
     7921 attgaaaata gcccctttca caaaataata ccagtaaatt gtcatataat taatgaccag
     7981 acctttacct gtaaatgatg tgatgttcat atacagaaga atgccaacaa tttacagatg
     8041 attttacaaa ttcttaattt tccttttcta aatggatttg ttcacacatt atctcttcaa
     8101 agcaattgaa ttttctggaa aagattggct gtgtgtgtaa aaggtgctac tgaggaattt
     8161 aatgtgacat ggaatgaagc aaacagcagc ctaagacagg atggcaggaa aaatgtcaca
     8221 tgtttaaaat aagagtgaaa ccaacgacct ggacttatag agactgtgtg tccttaggca
     8281 aagctcttgt acaaacagtg cttgtgactg tgaaattcgg ggtttgcctg gattgtgtta
     8341 aatgtgtgct tggatgtatc ataaaaaaga atcggttctc tgtatttgac attttctgta
     8401 atttatctaa agttacaact ggctatgcct gatttttatc actattgaat caatcatttt
     8461 aaagaatgtt aagtctgctg atttggagag ctcatttaca tttattttga atgtctctta
     8521 tttaacacaa ctgatttaag ctttagatct gcgaagatac ggccacgggt gctcttgatc
     8581 ctgtggctga ttttggactg tgctgctcgc agctgctgat gaatcacata cttcctccat
     8641 tttcttccac tgattgactg ttataatttc cctaatttcc aggtcaaggt gctgtgcatt
     8701 gtggtaatag atgtgacatg attgcattca ttttatgttt caggttcagg gggaggtgtg
     8761 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct
     8821 agatcagatc cactagttct agagcggcca ttaccctgtt atccctaggc cgccaccgcg
     8881 gtggagctcc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     8941 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     9001 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     9061 gcgcagcctg aatggcgaat ggaaattgta agcgttaata ttttgttaaa attcgcgtta
     9121 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     9181 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     9241 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     9301 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     9361 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     9421 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     9481 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca
     9541 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     9601 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     9661 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     9721 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     9781 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     9841 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     9901 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     9961 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
    10021 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
    10081 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
    10141 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
    10201 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
    10261 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
    10321 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
    10381 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
    10441 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    10501 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
    10561 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    10621 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    10681 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    10741 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    10801 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
    10861 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    10921 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    10981 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    11041 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    11101 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    11161 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    11221 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    11281 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    11341 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    11401 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    11461 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    11521 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
    11581 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
    11641 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
    11701 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tgggtaccgg gccccccctc
    11761 gaattaccct gttatcccta tcgagcaaaa tcaatcctaa tgtaaaatag tatacgttca
    11821 tcattataat gcaaacactg tagaactgtt gtgttgtgcc ttgcatttca ttctcagttt
    11881 cttatcgtct gtctcttttt acgacagagc tagaaaaaac tagccttcat ttgccatcgc
    11941 cgtatgcaaa tccactccag aactcaacag gtttgttctg cggcgggtgt aagtgtgaac
    12001 accggcatgt aagtgcgctt gagcaggaga ataaagcgct cagagacacc ggaggaatcc
    12061 tttattttgc gttgcgcctc aggggattcg ctgagccttc tcttcatggc cctgctcttc
    12121 agacacaggt gctccgctgt ttgtgttttt cactgttctt cag
//
