LOCUS       sequence-443798-        7736 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human androgen receptor in pCMV5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Lubahn DB, Joseph DR, Sullivan PM, Willard HF, French FS, Wilson EM
  TITLE     Cloning of human androgen receptor complementary DNA and 
            localization to the X chromosome.
  JOURNAL   Science. 1988 Apr 15;240(4850):327-30.
  PUBMED    3353727
REFERENCE   2  (bases 1 to 7736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1988-04-15"; volume: "240(4850)"; pages: "327-30"
COMMENT     Sequence Label: pCMV-hAR
FEATURES             Location/Qualifiers
     source          1..7736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(701..904)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(730..750)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        905..1284
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(1445..1462)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1445..1461)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1454..1476)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(1602..2057)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1739..1760)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1951..1970
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2084..2188
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2189..2257,2258..3049)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2407..2426)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3220..3808
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3709..3728
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3962..3979
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4096..4117
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4132..4137,4138..4155,4156..4162)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4170..4186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4175..4197
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4194..4210
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4194..4210
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4224..4243
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4224..4242
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4281..4610)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4295..4430
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4595..4615
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    4639..5261
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     5043..5062
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 tgctggcagc tgctgcggca gccccttgct ggcggccacg gcggctccag gctctgggac
       61 gcaacctctc tcggggtggc actccagggc cgactgcggc tgtgaaggtt gctgttcctc
      121 atccaggacc aggtagcctg tggggcctct acgatgggct tggggagaac catcctcacc
      181 ctgctgctgc tgctgctgcc tggggctagt ctcttgctgc tgctgctgct gctgctgctg
      241 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgca gcagcagcaa
      301 actggcgccg ggaggtgctg cgctcgcggc ctctgggtgc ctggggcccg ggttctggat
      361 cacttcgcgc acgctctgga acagattctg gaaagctcct cggtaggtct tggacggcgg
      421 ccgagggtag acccttccca gccctaactg cacttccatc cttgagcttg gctgaatctt
      481 ccacctactt cccttacccc gcctcccctt ctcttgctca gaagagttca acaggctgtg
      541 atgatgcggt agtcgctgca gctagctgcc cacctgcggg aagctccaga gaaggaaact
      601 tgcgggagca accacgcaaa aagcgctctg acagcctcaa agtctcgtgc agaagaagac
      661 acacggtgga cagatctgaa ttctgacggt tcactaaacg agctctgctt atatagacct
      721 cccaccgtac acgcctaccg cccatttgcg tcaacggggc ggggttatta cgacattttg
      781 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
      841 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt ggtgtactgc caaaaccgca
      901 tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccgta
      961 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
     1021 gcggacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
     1081 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
     1141 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
     1201 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt
     1261 aataactagt caataatcaa tgtcaacatg gcggtcatat tggacatgag ccaatataaa
     1321 tgtacatatt atgatataga tacaacgtat gcaatggcca atagccaata ttgatttatg
     1381 ctatataacc aatgactaat atggctaatt gccaatattg attcaatgta tagatcagct
     1441 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     1501 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     1561 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gaaattgtaa gcgttaatat
     1621 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     1681 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     1741 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     1801 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     1861 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     1921 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     1981 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     2041 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2101 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2161 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     2221 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     2281 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     2341 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     2401 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     2461 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     2521 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     2581 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     2641 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     2701 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     2761 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     2821 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     2881 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     2941 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     3001 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     3061 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     3121 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     3181 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3241 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3301 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3361 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3421 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3481 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3541 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3601 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3661 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3721 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3781 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3841 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3901 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3961 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     4021 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     4081 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     4141 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     4201 cagctatgac atgattacga atttaatacg actcactata gggaattaac tttctggttt
     4261 ttcagttcct cgaggagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     4321 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
     4381 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
     4441 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     4501 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
     4561 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attccacagc
     4621 caagctggcc gcgtacccaa ttcaacaggc atctactgag tggacccaac gcatgagagg
     4681 acagtgccaa gcaagcaact caaatgtccc accggttggg catggccagg tagcctatgc
     4741 tgtgtctgga cgtcctcctg ctggtatagt tattttaaaa tcagaaggac agggaaggga
     4801 gcagtggttc acgcctgtaa tcccagcaat ttgggaggcc aaggtgggta gatcacctga
     4861 gattaggagt tggagaccag cctggccaat atggtgaaac cccgtctcta ccaaaaaaac
     4921 aaaaattagc tgagcctggt catgcatgcc tggaatccca acaactcggg aggctgaggc
     4981 aggagaatcg cttgaaccca ggaggcggag attgcagtga gccaagattg tgccactgca
     5041 ctccagcttg gttcccaata gaccccgcag gccctacagg ttgtcttccc aacttgcccc
     5101 ttgctccata ccacccccct ccaccccata atattataga aggacaccta gtcagacaaa
     5161 atgatgcaac ttaattttat taggacaagg ctggtgggca ctggagtggc aacttccagg
     5221 gccaggagag gcactgggga ggggtcacag ggatgccacc cgggatcctc tagagtcgac
     5281 ctgcaggcat gcaagctcat gacagactgt acatcaatag aggaaattcc ccaaggcact
     5341 gcagaggagt agtgcagagt tataacaggc agaagacatc tgaaaggggg catgagctgg
     5401 ggtggggaaa tagggtttcc aatgcttcac tgggtgtgga aatagatggg cttgactttc
     5461 ccagaaagga tcttgggcac ttgcacagag atgatctctg ccatcatttc cggaaagtcc
     5521 acgctcacca tgtgtgactt gattagcagg tcaaaagtga actgatgcag ctctctcgca
     5581 ataggctgca cggagtccag gagcttggtg agctggtaga agcgtcttga gcaggatgtg
     5641 ggattttttc ttttgcatgc aatgatacga tcgagttcct tgatgtagtt cattcgaagt
     5701 tcatcaaaga atttttgatt tttcagccca tccactggaa taatgctgaa gagtagcagt
     5761 gctttcatgc acaggaattc ctggggggtg atttggagcc atccaaactc ttgagagagg
     5821 tgcctcattc ggacacactg gctgtacatc cgggacttgt gcatgcggta ctcattgaaa
     5881 accagatcag gggcgaagta gagcatcctg gagttgacat tggtgaagga tcgccagccc
     5941 atggcaaaca ccatgagccc catccaggag tactgaatga cagccatctg gtcgtccacg
     6001 tgtaagttgc ggaagccagg caaggccttg gcccacttga ccacgtgtac aagctgtctc
     6061 tctcccagtt cattgaggct agagagcaag gctgcaaagg agtcgggctg gttgttgtcg
     6121 tgtccagcac acactacacc tggctcaatg gcttccagga cattcagaaa gatgggctga
     6181 cattcatagc cttcaatgtg tgacactgtc agcttctggg ttgtctcctc agtggggctg
     6241 gtggtgctgg aagcctctcc ttcctcctgt agtttcagat taccaagttt cttcagcttc
     6301 cgggctccca gagtcatccc tgcttcataa catttccgaa gacgacaaga tggacaattt
     6361 ttccttcgga atttatcaat agtgcaatca tttctgctgg cgcacaggta cttctgtttc
     6421 ccttcagcgg ctcttttgaa gaagaccttg cagcttccac atgtgagagc tccatagtga
     6481 cacccagaag cttcatctcc acagatcagg caggtcttct ggggtggaaa gtaatagtca
     6541 atgggcaaaa catggtccct ggcagtctcc aaacgcatgt ccccgtaagg tccggagtag
     6601 ctatccatcc aggggcccat ttcgcttttg acacaagtgg gactgggata gggcactctg
     6661 ctcaccatgc cgccagggta ccacacatca ggtgcggtga agtcgctttc ctggcccgcc
     6721 agcccctgag ggggccgagt gtagccgtag ggggctacag ctcccgcctc gccgccgccg
     6781 ccgccgccgc cgccgccgcc gccgccgccg ccgccgccgc caccaccccc accaccacca
     6841 cacggtccat acaactggcc ttcttcggct gtgaagagag tgtgccagga tgaggaagcg
     6901 gcggctgagg gtgacccaga accgggtccc gctgcacccg cgccatgcag gctcgccagg
     6961 tccccatagc ggcactgcgc cgccgcagcc gcccaggcgc tgccgtagtc cagcgggttc
     7021 tccagcttga tgcgagcgtg gggatgggga ggcggcggag ggggcggcgg tccggccaga
     7081 gccagtggaa agttgtagta gtcgcgactc tggtacgcag ctgcctcgtc cagtgctccg
     7141 gacttgtaga gagacagggt agacggcagt tcaagtgtcc cggagctccc tgctgcagcg
     7201 ctgccagagc agcctaggct ctcgccttct agccctttgg tgtaacctcc cttgaaaggg
     7261 gaatactcag cagtatcttc agtgctcttg cctgcgctgt cgtctagcag agaacctttg
     7321 cattcggcca atggggcaca aggagtggga cgcacagcgg gtggaactcc caaaagtggg
     7381 gcgtacatgc aatccccccg aagctgttcc cctggactca gatgctccaa cgcctccaca
     7441 cccaggccca tggacaccga cactgcctta cacaactcct tggcgttgtc agaaatggtc
     7501 gaagtgcccc ctaagtaatt gtccttggag gaagtgggag cccccgaggc ctccctcgct
     7561 ctcccgctgc tgctgccttc ggatactgct tcctgctgct gttgctgaag gagttgcatg
     7621 gtgctggcct cgctcaggat gtctttaagg tcagcggagc agctgcttaa gccggggaaa
     7681 gtggggccca gcagggacaa cgtggatggg gcagctgagt catcctcgtc cggagg
//
