LOCUS       sequence-443799-        8885 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of a lysosomal tag (Tmem192-Flag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8885)
  AUTHORS   Abu-Remaileh M, Wyant GA, Kim C, Laqtom NN, Abbasi M, Chan SH, 
            Freinkman E, Sabatini DM
  TITLE     Lysosomal metabolomics reveals V-ATPase- and mTOR-dependent 
            regulation of amino acid efflux from lysosomes.
  JOURNAL   Science. 2017 Nov 10;358(6364):807-813. doi: 
            10.1126/science.aan6298. Epub 2017 Oct 26.
  PUBMED    29074583
REFERENCE   2  (bases 1 to 8885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aan6298"; journalName: "Science"; date: 
            "2017-11-10- 10"; volume: "358"; issue: "6364"; pages: "807-813"
COMMENT     Sequence Label: pLJC5-Tmem192-2xFlag
FEATURES             Location/Qualifiers
     source          1..8885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    435..560
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             607..787
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(788..1014)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1042..1062)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1042..1060)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1081..1097)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1081..1097)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1094..1116)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1105..1121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1129..1135,1136..1153,1154..1159))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1174..1195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1312..1329)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1483..2071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1563..1582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2242..3033,3034..3102))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2865..2884
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3103..3207)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3233..3688)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3370..3391)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3582..3601
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3815..3837
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3829..3846
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3830..3846
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3856..3875
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3856..3874
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3895..4030
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4070..4191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4081..4100)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4135..4154
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4263..4496
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(4624..5223)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4707..4727)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5204..5223
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5245..5755)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5308..5326)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    5804..5921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5966..6013)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     regulatory      6834..6843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6865..8076)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(7009..7028)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             complement(8365..8409)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8594..8827
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc caattgtccc
       61 tcatatctcc tcctccaggt ctgaagatca gctgctgctt gctgtgcggt ggtcttactt
      121 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
      181 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
      241 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
      301 aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
      361 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
      421 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
      481 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
      541 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
      601 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
      661 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
      721 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
      781 gagacccgtt tatgtatcga gctaggcact taaatacaat atctctgcaa tgcggcaatt
      841 cagtggttcg tccaatccat gtcagacccg tctgttgcct tcctaataag gcacgatcgt
      901 accaccttac ttccaccaat cggcatgcac ggtgcttttt ctctccttgt aaggcatgtt
      961 gctaactcat cgttaccatg ttgcaagact acaagagtat tgcataagac tacattaagc
     1021 ttgcagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     1081 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     1141 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     1201 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1261 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1321 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1381 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1441 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1501 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1561 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1621 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1681 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1741 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1801 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1861 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1921 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1981 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2041 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2101 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2161 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2221 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2281 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2341 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     2401 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2461 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2521 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2581 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     2641 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     2701 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     2761 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     2821 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     2881 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     2941 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3001 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3061 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3121 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3181 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     3241 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     3301 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     3361 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     3421 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     3481 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     3541 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     3601 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     3661 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     3721 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     3781 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     3841 gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacgtc cctaggcctc
     3901 caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg
     3961 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
     4021 ggggcgggat gggcggagtt aggggcggga tagctagagc cagacatgat aagatacatt
     4081 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     4141 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt tcctctcact
     4201 ctctgatatt catttctttg caagttataa atactgaata ataagatgac atgaactact
     4261 actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc
     4321 acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag
     4381 cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga
     4441 cccagtacaa gcaaaaagca gatcttgtct tcgttgggag tgaattagcc cttccagtcc
     4501 ccccttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa
     4561 aggtaccgat gcatggggtc gtgcgctcct ttcggtcggg cgctgcgggt cgtggggcgg
     4621 gcgtcaggca ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac
     4681 gtcggcggtg acggtgaagc cgagccgctc gtagaagggg aggttgcggg gcgcggaggt
     4741 ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc actccgggga gcacgacggc
     4801 gctgcccaga cccttgccct ggtggtcggg cgagacgccg acggtggcca ggaaccacgc
     4861 gggctccttg ggccggtgcg gcgccaggag gccttccatc tgttgctgcg cggccagccg
     4921 ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac
     4981 gctctccggc gtggtccaga ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc
     5041 gatgtcgagc ccgacgcgcg tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg
     5101 gcggtccgga tcgacggtgt ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt
     5161 gcgtacggcc ctggggacgt cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt
     5221 catggtaagc tccggtggat ccccctgggg agagaggtcg gtgattcggt caacgaggga
     5281 gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg gaacaccgcg cgggcaggaa
     5341 cagggcccac actaccgccc cacaccccgc ctcccgcacc gccccttccc ggccgctgct
     5401 ctcggcgcgc cctgctgagc agccgctatt ggccacagcc catcgcggtc ggcgcgctgc
     5461 cattgctccc tggcgctgtc cgtctgcgag ggtactagtg agacgtgcgg cttccgtttg
     5521 tcacgtccgg cacgccgcga accgcaagga accttcccga cttaggggcg gagcaggaag
     5581 cgtcgccggg gggcccacaa gggtagcggc gaagatccgg gtgacgctgc gaacggacgt
     5641 gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc ccggaaccac gcccagagca
     5701 gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg cgcaacccca accccctcga
     5761 gccgcggcca aagtggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg
     5821 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
     5881 cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca
     5941 tttgtctcga ggtcgagaat tcttacttgt cgtcatcgtc tttgtagtcc ttgtcgtcat
     6001 cgtctttgta gtcactacct ccacctcccg ttctacttgg ctgacagccc aggtctgagg
     6061 aagtgagagc caacaatcgc ttactcagca gcgcattgtg tcgcttcagg tattcaatgg
     6121 tgtctccttg cttttcaaca atttcttcta ggcttgaaat agttctgaat ccagtctccg
     6181 aggtaatatt gctggggtaa gcatagattt tttcttcttc aagtatatca ggctctggtt
     6241 tagctttatt aaatctccgg attttcactg tgtaaatgag gagacatatc agggaacaga
     6301 tgagttccag tgccaagatg gccagaatga ggtcaagata caatctgcca ggctctggga
     6361 aggagtgctg catgcacagt atgaggagaa gcactgtgtt gccagaggac tgtatcatca
     6421 acgcaagtct cttgagatgc cttgttgatc ggtagatcaa gttatagcct cggtttctga
     6481 ttttgctgtg gtgatactgg atgtagcatt caaggagtaa atggagaatc cacaaaataa
     6541 ctttcccaag gattataacc gtctgaactt tcaatgggtt tgtgtaattt cctgggcact
     6601 tgtcctcatt tggattagga taagaacaaa gcacacctgt taaaaatgct aaaacaacaa
     6661 acacgagatg aataaaccac agaagattca ctatgatgac tgtaggaaga ggatggaatc
     6721 ggggtctaaa gtgagcttgt aatgagtggt gtgggagaag ctgggcatcc agaagtgggt
     6781 cgtcttcaat actctgggtg atatccaagg aaccgtcctc catcctgccc cccgccgcca
     6841 tggtaccggt agcgctagcg cttcgtctaa caaaaaagcc aaaaacggcc agaatttagc
     6901 ggacaattta ctagtctaac actgaaaatt acatattgac ccaaatgatt acatttcaaa
     6961 aggtgcctaa aaaacttcac aaaacacact cgccaacccc gagcgcatag ttcaaaaccg
     7021 gagcttcagc tacttaagaa gataggtaca taaaaccgac caaagaaact gacgcctcac
     7081 ttatccctcc cctcaccaga ggtccggcgc ctgtcgattc aggagagcct accctaggcc
     7141 cgaaccctgc gtcctgcgac ggagaaaagc ctaccgcaca cctaccggca ggtggcccca
     7201 ccctgcatta taagccaaca gaacgggtga cgtcacgaca cgacgagggc gcgcgctccc
     7261 aaaggtacgg gtgcactgcc caacggcacc gccataactg ccgcccccgc aacagacgac
     7321 aaaccgagtt ctccagtcag tgacaaactt cacgtcaggg tccccagatg gtgccccagc
     7381 ccatctcacc cgaataagag ctttcccgca ttagcgaagg cctcaagacc ttgggttctt
     7441 gccgcccacc atgcccccca ccttgtttca acgacctcac agcccgcctc acaagcgtct
     7501 tccattcaag actcgggaac agccgccatt ttgctgcgct ccccccaacc cccagttcag
     7561 ggcaaccttg ctcgcggacc cagactacag cccttggcgg tctctccaca cgcttccgtc
     7621 ccaccgagcg gcccggcggc cacgaaagcc ccggccagcc cagcagcccg ctactcacca
     7681 agtgacgatc acagcgatcc acaaacaaga accgcgaccc aaatcccggc tgcgacggaa
     7741 ctagctgtgc cacacccggc gcgtccttat ataatcatcg gcgttcaccg ccccacggag
     7801 atccctccgc agaatcgccg agaagggact acttttcctc gcctgttccg ctctctggaa
     7861 agaaaaccag tgccctagag tcacccaagt cccgtcctaa aatgtccttc tgctgatact
     7921 ggggttctaa ggccgagtct tatgagcagc gggccgctgt cctgagcgtc cgggcggaag
     7981 gatcaggacg ctcgctgcgc ccttcgtctg acgtggcagc gctcgccgtg aggagggggg
     8041 cgcccgcggg aggcgccaaa acccggcgcg gaggccagat cccgctcgag gctagtctcg
     8101 tgatcgatac cgtcgagatc cgttcactaa tcgaatggat ctgtctctgt ctctctctcc
     8161 accttcttct tctattcctt cgggcctgtc gggtcccctc ggggttggga ggtgggtctg
     8221 aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac
     8281 tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat accacagcca
     8341 atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg
     8401 ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta
     8461 attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga
     8521 tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca
     8581 accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc
     8641 ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc
     8701 cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt
     8761 cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa
     8821 agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg
     8881 tgcta
//
