LOCUS       sequence-443803-        4180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral packaging plasmid; Contains Rev; also 
            requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid 
            (Addgene#12259).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L
  TITLE     A third-generation lentivirus vector with a conditional packaging 
            system.
  JOURNAL   J Virol. 1998 Nov . 72(11):8463-71.
  PUBMED    9765382
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1998 Nov . 72(11):8463-71."
COMMENT     Sequence Label: pRSV-Rev
FEATURES             Location/Qualifiers
     source          1..4180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    32..53
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(170..187)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(341..929)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(421..440)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1100..1891,1892..1960))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1723..1742
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1961..2065)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2133..2151
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2189..2211)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2311..2330
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2347..2802)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2484..2505)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2696..2715
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3133..3150
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3134..3150
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3234..3584)
                     /codon_start=1
                     /gene="rev"
                     /product="human immunodeficiency virus 1 (HIV-1) Rev 
                     protein"
                     /label=Rev
                     /note="enhances nuclear export of unspliced and 
                     incompletely spliced viral mRNAs"
                     /translation="MAGRSGDSDEDLLKAVRLIKFLYQSNPPPNPEGTRQARRNRRRRW
                     RERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILV
                     ESPTILESGAKE"
     primer_bind     complement(3678..3702)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(3713..3974)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4119..4135)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4119..4135)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4132..4154)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4143..4159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4167..4173,4174..4180,1..11,12..17))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
       61 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      121 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      181 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      241 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      301 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      361 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      421 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      481 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      541 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      601 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      661 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      721 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      781 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      841 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      901 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      961 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     1021 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1081 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1141 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1201 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     1261 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1321 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1381 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1441 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1501 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1561 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1621 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1681 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1741 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1801 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1861 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1921 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1981 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     2041 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     2101 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     2161 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     2221 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     2281 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     2341 accataaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2401 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaatagc
     2461 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2521 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2581 cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     2641 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     2701 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     2761 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtactatggt tgctttgacg
     2821 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     2881 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2941 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     3001 ctgatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
     3061 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagatc tcccaggctc
     3121 agatcacgac gttgtaaaac gacggccagt gccaagcttc ttgtactact tctataaccc
     3181 tatctgtccc ctcagctact gctatagctg tggcattgag caagctaaca gcactattct
     3241 ttagctcctg actccaatat tgtaggagat tccaccaata tttgagggct tcccaccccc
     3301 tgcgtcccag aagttccaca atcctcgtta caatcaagag taagtctctc aagcggtggt
     3361 agctgaagag gcacaggctc cgcagatcgt cccagataag tgctaaggat ccgttcacta
     3421 atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct tcgggcctgt
     3481 cgggtcccct cgggattggg aggtgggttg ctttgataga gaaacttgat gagtctgact
     3541 gccttgagga ggtcttcgtc gctgtctccg cttcttcctg ccataggaga tgcctaatcg
     3601 actagcttcg aggggaggct ggatcggtcc cggtgtcttc tatggaggtc aaaacagcgt
     3661 ggatggcgtc tccaggcgat ctgacggttc actaaacgag ctcgagcttg gaggtgcaca
     3721 ccaatgtggt gaatggtcaa atggcgttta ttgtatcgag ctaggcactt aaatacaata
     3781 tctctgcaat gcggaattca gtggttcgtc caatccatgt cagacccgtc tgttgccttc
     3841 ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg tgctttttct
     3901 ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac aagagtattg
     3961 cataagacta catttccccc tccctatgca aaagcgaaac tactatatcc tgaggggact
     4021 cctaaccgcg tacaaccgaa gccccgcttt tcgcctaaac acaccctagt cccctcagat
     4081 acgcgtatat ctggcccgta catcgaattc gtaatcatgt catagctgtt tcctgtgtga
     4141 aattgttatc cgctcacaat tccacacaac atacgagccg
//
