LOCUS       sequence-443810-        5985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gsdA disruption cassette via integration of pyrG from A. nidulans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Matthias Steiger
  TITLE     Regulation of the Glucose-6-phosphate dehydrogenase encoding gene 
            gsdA and its impact on growth and citric acid production in 
            Aspergillus niger
  JOURNAL   PLOS One
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLOS One"
COMMENT     Sequence Label: pSF2
FEATURES             Location/Qualifiers
     source          1..5985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(526..559)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3222..3255)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(3900..3916)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3900..3916)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      3964..3995
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        3996..4098
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             4099..4914
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4172..4191)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      4938..4965
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(4977..5564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5057..5076)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5604..5621
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5605..5621
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttatattcgt caagctatag cctagctaac atggatgttt tacgtagcac catggagctc
       61 aaagatgaca ctgtcatcat agtactgggt gcctccggag atcttgcaaa gaagaagacc
      121 gtcagtgacg accccctgat tcatgttgac ctgacagaaa gctaaccttt tacagttccc
      181 ggcccttttc ggccttgtat gtcctctccc agatccaatt gcagtttgac tcaccagtat
      241 ggttgctgat ttgcgcttcc agtatcgcaa caagttcctc cccaagggaa tcaagatcgt
      301 cggatatgcc cggacaaaca tggaccatga ggagtacctg aggcgtgtgc gctcatacat
      361 caagacccct accaaggaaa tcgaagagca gctggacagc ttctgccagt tctgcaccta
      421 catttccggt caatatgaca aggatgactc gttcatcaac ctcaacaagc acctcgagga
      481 gattgagaag ggccagaagg agcagaacag aatctactac atatgataac ttcgtatagc
      541 atacattata cgaagttatc ccatgctaat catataacga tctttattat agatatgcca
      601 tcgccgtttc gctaggcgca atccctgtcc tgggattcat ccatggtgtc ctcgtcggct
      661 cttttcgcaa ggccgctggc gtgccgtacc cccacgccta tgccagcatt gagcaatgta
      721 aagctaacgt gcgtgagccc aagaaactaa atacctatag caaaacagat tgtgttccaa
      781 gagagagtac taaatgacgt ttgtgaacag cccaaagcct acaaattcaa ctgcgcacaa
      841 cgcgcccacg gcaacttcct cgagaacgcg ccgcagacaa tgctctctat cctggtggca
      901 ggcgtcaagt acccagaggc agcagcgggc ttaggagcgg cctgggttgt tctccgcacc
      961 ctctacatgc tgggctatat ttatagcgac aagccgaacg gcaccggcag gtacaatggt
     1021 tcgctgtact tgcttgcgca agcgggtctt tggggattga gcgcatttgg tgttgcaaag
     1081 gatttgatgt aaatgtagtc gacatcttag cacagagggg agagttgata aaatgtggtc
     1141 tgtttgaatg atagtcgggt tcgtgaccta tattcgtgat agtggagata ggtctgcgcc
     1201 tatcttatcg ggccggagca aaaattccac cgcagcgggg tgagttttcg ttatacagcc
     1261 atcccacttc cagcttcaaa ttgtcagttt aatccagccc aattcaatca ttggagaacc
     1321 gccatcatgt cgtcgaagtc ccacctcccc tacgcaattc gcgcaaccaa ccatcccaac
     1381 cctttaacat ctaaactctt ctccatcgcc gaggagaaga aaaccaacgt caccgtctcc
     1441 gcagacgtta ctacttccgc cgagctcctc gatcttgctg accgtacatc ctgcaccaat
     1501 gcccctccag gataacaaat agctgatgcg tagtgagtac aggcctaggc ccctatatcg
     1561 cagttctgaa aacccacatc gacatcctca ccgatctcac cccgtcgacc ctttcctcgc
     1621 tccaatccct cgcgacaaag cacaacttcc tcatctttga ggaccgcaag ttcatcgaca
     1681 tcggcaacac cgtgcaaaag cagtaccacg gtggcgctct ccgcatctcc gaatgggcac
     1741 acatcatcaa ctgcgccatc ctgccgggcg aagggatcgt cgaggccctc gcacagacaa
     1801 ccaagtctcc tgactttaaa gacgcgaatc aacgaggcct cctgattctt gccgagatga
     1861 cgagtaaggg atctcttgcg acaggggagt acacggcacg ctcggttgag tacgcgcgga
     1921 agtataaggg gtttgtgatg ggattcgtga gtacaagggc gttgagtgag gtgctgcccg
     1981 aacagaaaga ggagagcgag gattttgtcg tctttacgac tggggtgaat ctgtcggata
     2041 agggggataa gctggggcag cagtatcaga cacctgggtc ggcggttggg cgaggtgcgg
     2101 actttatcat tgcgggtagg ggcatctata aggcggacga tccagtcgag gcggttcaga
     2161 ggtaccggga ggaaggctgg aaagcttacg agaaaagagt tggactttga gtgtgagtgg
     2221 aaatgtgtaa cggtattgac taaaagggat ccatatgttt attgcagcca gcatagtatt
     2281 accagaaaga gcctcactga cggctctagt agtattcgaa cagatattat tgtgaccagc
     2341 tctgaacgat atgctcccta atctggtaga caagcactga tctacccctt ggaacgcagc
     2401 atctaggctc tggctgtgct ctaaccctaa ctagacgatt gatcgcagac catccaatac
     2461 tgaaaagtct ctatcagagg aaatccccaa cattgtagta gtcaggttcc tttgtggctg
     2521 ggagagaatt ggttcgctcc actgattcca gttgagaaag tgggctagaa aaaagtcttg
     2581 aagattggag ttgggctgtg gttaagccgg cttttattga ccttatcatt tagcaaaata
     2641 tgggcagttg ctatcaggac cacatactct acccgaagct taaaggcaaa aagaaattct
     2701 gtatgtcctg cgaatcaaca ttcctcgtgt tatatgagcc caaggcgctg aaccaggaat
     2761 attagctacg cttgtggctc gcgaagcaat gatactccct tctgaagtgt gtattgagct
     2821 agttacatta gtggcacatc ttaacaccag cacattggca tatttaggat actattgata
     2881 atggaattca actatcttgc tttatagccg actacagctt cggaacgcaa tccttcttta
     2941 cgtaaatgtg aaaatgctct tagacagctt gaaaggccaa aaaatctccc agaaaaaaaa
     3001 aagagaatta gagaaaatcc agtgggtata tagctatgga tgccctcaat tatcctgtat
     3061 cttcagatgt tccacgagat ccacttagaa cataaggcaa ttcctatcct caccatctca
     3121 tctgttttgc ttctctttag gaaacacatg tttctactga cctcgcccct ttccttgatc
     3181 atttccactg tccagtgatt gtctctagaa ttagagctct gataacttcg tatagcatac
     3241 attatacgaa gttatgagga gatcatcccc atggaatacc cctacggtac gtgcacttct
     3301 tgcaatttgt ctaaatcgct tacatactga ccaacgcgca ggctcccgcg gacccgccgt
     3361 ccttgatgac ttcaccgcgt ccttcggcta caagttcagc gatgctgctg gctaccagtg
     3421 gcccttgact tccaccccca accgtctgta aataagggcg gtcggcaggt tatgacggat
     3481 gaggatgaaa aaaaaattat tgccaaaaaa ggctaaaaaa aagatgttaa tgcgattgat
     3541 tttcggtcga gaatcatggt atgacggggc atctgggatg atatgacaga aatgaagcac
     3601 tcgggactat ttatcggtcg gctgggcaat aactggagtt atctattcgc aacccctttt
     3661 tagaaacgaa tgcagagaac gtaacgaacc acccccggtc ggttgggacc gccgctgtct
     3721 tcaccggaag ctggaagtag tcgcgtgacc gtagccagac ggtacctgta ttattggaga
     3781 tacccttgcc ttgagcctta gtgtctttgg tctcatggcg tcttcgaatt cgcgcgtgat
     3841 cttacggcat tatacgtatg atcggtccac gatcagctag attatctagt cagcttgatg
     3901 tcatagctgt ttcctgaggc tcaatactga ccatttaaat catacctgac ctccatagca
     3961 gaaagtcaaa agcctccgac cggaggcttt tgacttgatc ggcacgtaag aggttccaac
     4021 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca
     4081 ggagctaagg aagctaaaat gagccatatt caacgggaaa cgtcttgctt gaagccgcga
     4141 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     4201 caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg
     4261 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag gctaaactgg
     4321 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     4381 tggttactca ccactgcgat cccagggaaa acagcattcc aggtattaga agaatatcct
     4441 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     4501 cctgtttgta attgtccttt taacggcgat cgcgtatttc gtctcgctca ggcgcaatca
     4561 cgaatgaata acggtttggt tggtgcgagt gattttgatg acgagcgtaa tggctggcct
     4621 gttgaacaag tctggaaaga aatgcataaa ctcttgccat tctcaccgga ttcagtcgtc
     4681 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     4741 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     4801 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     4861 aatcctgata tgaataaatt gcagtttcac ttgatgctcg atgagttttt ctaatgagga
     4921 cctaaatgta atcacctggc tcaccttcgg gtgggccttt ctgcgttgct ggcgtttttc
     4981 cataggctcc gcccccctga cgagcatcac aaaaatcgat gctcaagtca gaggtggcga
     5041 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5101 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5161 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5221 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5281 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5341 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5401 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttacctcg
     5461 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5521 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgattt
     5581 tctaccgaag aaaggcccac ccgtgtaaaa cgacggccag tttatctagt cagcttgatt
     5641 ctagctgatc gtggaccgga aggtgagcca gtgagttgat tgcagtccag ttacgctgga
     5701 gtctgaggct cgtcctgaat gatatgcggc ctcgaattcg aagacgcgga gagacgtgag
     5761 gattcaccac ccacgactct ccataacaag ccccgtcgcc caaacattca ctggcaggct
     5821 tcccgctttc cattattctt caattcgtca ccaggattac tccttcgggc ttaacgaaag
     5881 gactatcctt ctgactcacc accaaccctc actgcccctc ctgcatgctg tagcggactg
     5941 cgggcaccga cctgcatcat cgatctacac ccgatccctg tgaca
//
