LOCUS       sequence-443811-        8847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Opto-T7RNAP(563).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Baumschlager A, Aoki SK, Khammash M
  TITLE     Dynamic Blue Light-Inducible T7 RNA Polymerases (Opto-T7RNAPs) for 
            Precise Spatiotemporal Gene Expression Control.
  JOURNAL   ACS Synth Biol. 2017 Nov 17;6(11):2157-2167. doi: 
            10.1021/acssynbio.7b00169. Epub 2017 Oct 18.
  PUBMED    29045151
REFERENCE   2  (bases 1 to 8847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00169"; journalName: "ACS Synth Biol"; date: 
            "2017-11-17- 17"; volume: "6"; issue: "11"; pages: "2157-2167"
COMMENT     Sequence Label: pAB202
FEATURES             Location/Qualifiers
     source          1..8847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..223
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             271..1221
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(1402..1488)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2478..2927)
                     /codon_start=1
                     /product="""positive Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=pMag
                     /note="rapidly heterodimerizes with nMagHigh1 upon blue 
                     light irradiation to form a complex with slow switch-off 
                     kinetics"
                     /translation="HTLYAPGGYDIMGYLRQIRNRPNPQVELGPVDTSCALILCDLKQK
                     DTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTMRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSMGFQCETE"
     RBS             2968..2988
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(3980..4429)
                     /codon_start=1
                     /product="""positive Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=pMag
                     /note="rapidly heterodimerizes with nMagHigh1 upon blue 
                     light irradiation to form a complex with slow switch-off 
                     kinetics"
                     /translation="HTLYAPGGYDIMGYLRQIRNRPNPQVELGPVDTSCALILCDLKQK
                     DTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTMRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSMGFQCETE"
     RBS             4470..4490
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      complement(5092..5178)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(5194..5643)
                     /codon_start=1
                     /product="""negative Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=nMagHigh1
                     /note="rapidly heterodimerizes with pMag or pMagFast2 upon 
                     blue light irradiation"
                     /translation="HTLYAPGGYDIMGYLDQIGNRPNPQVELGPVDTSCALILCDLKQK
                     DTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTIRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTIIPVRDETGEYRYSMGFQCETE"
     promoter        7479..7581
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             7582..8241
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7648..7667)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      8262..8356
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gagttataca cagggctggg atctattctt tttatctttt tttattcttt ctttattcta
       61 taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc ggaatttaca
      121 gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt acagataccc
      181 acaactcaaa ggaaaaggac tagtaattat cattgactag cccatctcaa ttggtatagt
      241 gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt caaagcaaat
      301 gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat tttatgctgt
      361 gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac ggtatcgttc
      421 acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta tggtgtatta
      481 gctaaagcaa ccagagagct gatgacgaga actgtggaaa tcaggaatcc tttggttaaa
      541 ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa attagaatta
      601 gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat aaaatataat
      661 ctggaacatg ttaagtcttt tgaaaacaaa tactctatga ggatttatga gtggttatta
      721 aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct tgatgaattt
      781 aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa ccaatgggtt
      841 ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt ggttgataag
      901 cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca aatggatctc
      961 gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc aacaaccatt
     1021 acatcagatt cctacctaca taacggacta agaaaaacac tacacgatgc tttaactgca
     1081 aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag taagtatgat
     1141 ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa ccacactaga gaacatactg
     1201 gctaaatacg gaaggatctg aggttcttat ggctcttgta tctatcagtg aagcatcaag
     1261 actaacaaac aaaagtagaa caactgttca ccgttacata tcaaagggaa aactgtccat
     1321 atggatccta gcaacaccag aacagcccgt ttgcgggcag caaaacccgt accctagctg
     1381 cagctaggtc tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     1441 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctctagat
     1501 tacgcgaacg cgaagtccga ctctaagatg tcacggaggt tcaagttacc tttagccgga
     1561 agtgctggca ttttgtccaa ttgagactcg tgcaactggt cagcgaactg gtcgtagaaa
     1621 tcagccagta catcacaaga ctcatatgtg tcaaccatag tttcgcgcac tgctttgaac
     1681 aggttcgcag cgtcagccgg aatggtaccg aaggagtcgt gaatcagtgc aaaagattcg
     1741 attccgtact tctcgtgtgc ccacactaca gtcttacgaa ggtggctacc gtcttggctg
     1801 tgtacaaagt taggagcgat accagactcc tgtttgtgtg catcaatctc gctatctttg
     1861 ttggtgttaa tggtaggctg taagcggaac tgaccgagga acatcaggtt caagcgcgtc
     1921 tgaataggct tcttgtattc ctgccacaca gggaaaccat caggagttac ccaatgcaca
     1981 gcgcaacgct tgcgaagaat ctctccagtc ttcttatctt tgacctcagc agccagcagc
     2041 ttagcagcag acttaagcca gttcattgct tcaaccgcag ctaccaccgt cacgctcaca
     2101 gattcccaaa tcagcttagc catgtatcca gcagcctgat tcggctgagt gaacatcaga
     2161 cccttgccgg aatcaatagc tggctgaatg gtatcttcca gcacttgttg acggaagccg
     2221 aactctttgg acccgtaagc cagcgtcatg actgaacgct tagtcacact gcgagtaaca
     2281 ccgtaagcca gccattgacc agccagtgcc ttagtgccca gcttgacttt ctcagagatt
     2341 tcaccagtgt tctcatcggt cacggtaact acttcgttat cggtcccatt gattgcgtct
     2401 gcttgtagaa tctcgttgac tttcttagca acaatcccgt agatgtcctg aacggtttca
     2461 ctacctccag aaccgccttc ggtttcgcac tggaatccca tggagtatct gtactctccg
     2521 gtttcatccc ggaccggaat catggtcaga aagttcacga accgctggcc gttcttctta
     2581 aagttgacca cctccacctg gacctcggcg ttccggtcga tggccttccg catggtgttg
     2641 atcgtgttcg agtccacata tttgcgagtc gactttggtt tcaccatccc gtcgggggac
     2701 tgcaggaatc tgcagttcct gccgagcacc tctgcattgc tgtatccggt catatacaag
     2761 aaggcttccg aggcgtagac gatcggagtg tccttttgct tcagatcgca caggatcagg
     2821 gcacatgacg tgtccacagg ccccagttcg acctgagggt ttgggcggtt cctaatctgc
     2881 cgcaaatatc ccataatgtc gtatcctcca ggggcgtaaa gagtgtgcat taattaattt
     2941 acctccttcc aaaaaaacgg gtatgacctc cttcttaaag ttaaacaaaa ttatttctag
     3001 attacgcgaa cgcgaagtcc gactctaaga tgtcacggag gttcaagtta cctttagccg
     3061 gaagtgctgg cattttgtcc aattgagact cgtgcaactg gtcagcgaac tggtcgtaga
     3121 aatcagccag tacatcacaa gactcatatg tgtcaaccat agtttcgcgc actgctttga
     3181 acaggttcgc agcgtcagcc ggaatggtac cgaaggagtc gtgaatcagt gcaaaagatt
     3241 cgattccgta cttctcgtgt gcccacacta cagtcttacg aaggtggcta ccgtcttggc
     3301 tgtgtacaaa gttaggagcg ataccagact cctgtttgtg tgcatcaatc tcgctatctt
     3361 tgttggtgtt aatggtaggc tgtaagcgga actgaccgag gaacatcagg ttcaagcgcg
     3421 tctgaatagg cttcttgtat tcctgccaca cagggaaacc atcaggagtt acccaatgca
     3481 cagcgcaacg cttgcgaaga atctctccag tcttcttatc tttgacctca gcagccagca
     3541 gcttagcagc agacttaagc cagttcattg cttcaaccgc agctaccacc gtcacgctca
     3601 cagattccca aatcagctta gccatgtatc cagcagcctg attcggctga gtgaacatca
     3661 gacccttgcc ggaatcaata gctggctgaa tggtatcttc cagcacttgt tgacggaagc
     3721 cgaactcttt ggacccgtaa gccagcgtca tgactgaacg cttagtcaca ctgcgagtaa
     3781 caccgtaagc cagccattga ccagccagtg ccttagtgcc cagcttgact ttctcagaga
     3841 tttcaccagt gttctcatcg gtcacggtaa ctacttcgtt atcggtccca ttgattgcgt
     3901 ctgcttgtag aatctcgttg actttcttag caacaatccc gtagatgtcc tgaacggttt
     3961 cactacctcc agaaccgcct tcggtttcgc actggaatcc catggagtat ctgtactctc
     4021 cggtttcatc ccggaccgga atcatggtca gaaagttcac gaaccgctgg ccgttcttct
     4081 taaagttgac cacctccacc tggacctcgg cgttccggtc gatggccttc cgcatggtgt
     4141 tgatcgtgtt cgagtccaca tatttgcgag tcgactttgg tttcaccatc ccgtcggggg
     4201 actgcaggaa tctgcagttc ctgccgagca cctctgcatt gctgtatccg gtcatataca
     4261 agaaggcttc cgaggcgtag acgatcggag tgtccttttg cttcagatcg cacaggatca
     4321 gggcacatga cgtgtccaca ggccccagtt cgacctgagg gtttgggcgg ttcctaatct
     4381 gccgcaaata tcccataatg tcgtatcctc caggggcgta aagagtgtgc attaattaat
     4441 ttacctcctt ccaaaaaaac gggtatgacc tccttcttaa agttaaacaa aattatttct
     4501 agagggaaac cgttgtggtc tcccatggag aaacagtaga gagttgcgat aaaaagcgtc
     4561 aggtaggatc cgctaatctt atggataaaa atgctacgaa gcggcgcgcc gatatctggc
     4621 gaaaatgaga cgttgatcgg cgcatgcaca gatgaaaacg gtgtaaaaaa gatagataca
     4681 tcagagcttt tacgagtttt tggtgcattc aaagctgttc accatgaaca gatcgacaat
     4741 gtaacagatg aacagcatgt aacacctaat agaacaggtg aaaccagtaa aacaaagcaa
     4801 ctagaacatg aaattgaaca cctgagacaa cttgttacag ctcaacagtc acacatagac
     4861 agcctgaaac aggcgatgct gcttatcgaa tcaaagctgc cgacaacacg ggagccagtg
     4921 acgcctcccg tggggaaaaa atcatggcaa ttctggaaga aatagcgctt tcagccggca
     4981 aaccggctga agccggatct gcgattctga taacaaacta gcaacaccag aacagcccgt
     5041 ttgcgggcag caaaacccgt accctagctg cagctaggtc tagggcggcg gatttgtcct
     5101 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg
     5161 agcctttcgt tttatttgat gcctcctagg ttattcggtt tcgcactgga atcccatgga
     5221 gtatctgtac tctccggttt catcccggac cggaatgatg gtcagaaagt tcacgaaccg
     5281 ctggccgttc ttcttaaagt tgaccacctc cacctggacc tcggcgttcc ggtcgatggc
     5341 cttccggatg gtgttgatcg tgttcgagtc cacatatttg cgagtcgact ttggtttcac
     5401 catcccgtcg ggggactgca ggaatctgca gttcctgccg agcacctctg cattgctgta
     5461 tccggtcata tacaagaagg cttccgaggc gtagacgatc ggagtgtcct tttgcttcag
     5521 atcgcacagg atcagggcac atgacgtgtc cacaggcccc agttcgacct gagggtttgg
     5581 gcggttccca atctgatcca aatatcccat aatgtcgtat cctccagggg cgtaaagagt
     5641 gtgacctcca gaaccgccag gaagcaagtt aaccgcgcga ccacctacct catctcggag
     5701 catcgcggag aagtgctgga tgccagagca agacccgtca aacgccagcg gaagggagca
     5761 gttatagctc aggccgtggt gctgtacccc agcgtactca aagcagaacg caaggaagca
     5821 gaacggagaa tcttgctcag cccaccaagt gttctccagt ggagacttag cgcaagccat
     5881 gatgttctcg tggttttcct caatgaactt gatgcgctca gggaacggaa ccttatcgac
     5941 acccgcacag tttgcaccgt ggattttcag ccagtagtaa ccttccttac cgattggttt
     6001 acctttcgcc agcgtaagca gtcctttggt catatcgtta ccttgcgggt tgaacattga
     6061 cacagcgtaa acacgaccgc gccagtccat gttgtaaggg aaccagatgg ccttatggtt
     6121 agcaaactta ttggcttgct caagcatgaa ctcaaggctg atacggcgag acttgcgagc
     6181 cttgtccttg cggtacacag cagcggcagc acgtttccac gcggtgagag cctcaggatt
     6241 catgtcgatg tcttccggtt tcatcgggag ttcttcacgc tcaatcgcag ggatgtcctc
     6301 gaccggacaa tgcttccact tggtgattac gttggcgacc gctaggactt tcttgttgat
     6361 tttccatgcg gtgttttgcg caatgttaat cgctttgtac acctcaggca tgtaaacgtc
     6421 ttcgtagcgc atcagtgctt tcttactgtg agtacgcacc agcgccagag gacgacgacc
     6481 gttagcccaa tagccaccac cagtaatgcc agtccacggc ttaggaggaa ctacgcaagg
     6541 ttggaacatc ggagagatgc cagccagcgc acctgcacgg gttgcgatag cctcagcgta
     6601 ttcaggtgcg agttcgatag tctcagagtc ttgacctact acgccagcat tttggcggtg
     6661 taagctaacc attccggttg actcaatgag catctcgatg cagcgtactc ctacatgaat
     6721 agagtcttcc ttatgccacg aagaccacgc ctcgccaccg agtagaccct tagagagcat
     6781 gtcagcctcg acaacttgca taaatgcttt cttgtagacg tgccctacgc gcttgttgag
     6841 ttgttcctca acgtttttct tgaagtgctt agcttcaagg tcacggatac gaccgaagcg
     6901 agcctcgtcc tcaatggccc gaccgattgc gcttgctaca gcctgaacgg ttgtattgtc
     6961 agcactggtt aggcaagcca gagtggtctt aatggtgatg tacgctacgg cttccggctt
     7021 gatttcttgc aggaactgga aggctgtcgg gcgcttgccg cgcttagctt tcacttcctc
     7081 aaaccagtcg ttgatgcgtg caatcatctt agggagtagg gtagtgatga gaggcttggc
     7141 ggcagcgtta tccgcaacct caccagcttt aagttgacgc tcaaacatct tgcggaagcg
     7201 tgcttcaccc atctcgtaag actcatgctc aagggccaac tgttcgcgag ctaaacgctc
     7261 accgtaatgg tcagccagag tgttgaacgg gatagcagcc agttcgatgt cagagaagtc
     7321 gttcttagcg atgttaatcg tgttcataga tctttacctc cttccaaaaa aacgggtatg
     7381 gagaaacagt agagagttgc gataaaaagc gtcaggtagg atccgctaat cttatggata
     7441 aaaatgctag acgtcgatat ctggcgaaaa tgagacgttg atcggcacgt aagaggttcc
     7501 aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt
     7561 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     7621 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     7681 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     7741 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattt
     7801 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     7861 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     7921 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     7981 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     8041 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     8101 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     8161 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     8221 gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt tgatagatcc
     8281 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
     8341 ttttattggt gagaatccaa gcactaggga cagtaagacg ggtaagcctg ttgatgatac
     8401 cgctgcctta ctgggtgcat tagccagtct gaatgacctg tcacgggata atccgaagtg
     8461 gtcagactgg aaaatcagag ggcaggaact gctgaacagc aaaaagtcag atagcaccac
     8521 atagcagacc cgccataaaa cgccctgaga agcccgtgac gggcttttct tgtattatgg
     8581 gtagtttcct tgcatgaatc cataaaaggc gcctgtagtg ccatttaccc ccattcactg
     8641 ccagagccgt gagcgcagcg aactgaatgt cacgaaaaag acagcgactc aggtgcctga
     8701 tggtcggaga caaaaggaat attcagcgat ttgcccgagc ttgcgagggt gctacttaag
     8761 cctttagggt tttaaggtct gttttgtaga ggagcaaaca gcgtttgcga catccttttg
     8821 taatactgcg gaactgacta aagtagt
//
