LOCUS       sequence-44384-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: TALE5
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(71..220)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(245..265)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caggattctc ctcgacgtca ccgcatgtta gcagacttcc tctgccctct ccactgcctc
       61 tagagttaat cagcatgtcc aggtcgaaat catcaagggc gtcactgccg agcatgtcga
      121 ggtcaaagtc atcaagggca tccgaaccaa gcatgtcaag gtcaaaatca tcgagggcgt
      181 cacttcccag catatccaga tcaaaatcgt ccaatgcgtc agcccgtccg gaaccgctgg
      241 cctccacctt tctcttcttc ttggggctag ctgacgcgcg agtttggtcc ccttcatgca
      301 tgggcgaggg cgcgtccaaa tccctctcca gcgagtctgc gaacgcatga aggctcgcct
      361 ggtcaggtgt ctgagttgac gtaggtgaag gcttcgcgcg tttcataccg ctcgcttgga
      421 gaatcctgtc ccaccgctgg gaggcgggag gcagtgtgcc cgagcgggcc tcgagttctg
      481 tgacaccgac tctacgaaag agctgcagca gtccgtgtct cgacatacca aattgagtca
      541 tggcgtcatc gaacgcttgt gcggggtggg agtgacactg gaagaatccg agcacgcgga
      601 ccacttgcgc gtgatctgcc actcgatgtg atgtcctctc gggaatcctt ctgttggtcc
      661 gcttaatcaa tgcaggcgcg tgcgggagcc ccttcttcac cgcatccaag gcgggtcgtc
      721 cgccgaggca ggccagcgct acaagatgat cattagtgag tgcggccagc gcggggtccg
      781 gcctcgaaag ctgggccacg attgactcca gtgcgggtct gcccccgata ttggatgcaa
      841 tagccactac ctgctcaggc gtgagtccgt gcgcttggca cagc
//
