LOCUS       sequence-443883-        5934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This binary vector expresses mushroom luciferase (nnLuz) with the 
            truncated 35sCAMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5934)
  TITLE     Curtis lab plasmids
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLSU1/t35s[]:nnLuz:NosT
FEATURES             Location/Qualifiers
     source          1..5934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..195
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     658..678
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        667..712
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             complement(1205..1216)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      1568..1820
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1996..2015)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2007..2822
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2007..2822
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2080..2099)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2908..3496
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3397..3416
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3738..4367
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4796..5869
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELVDWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatgatat cgcatgcgga aacctcctcg gattccattg
      421 cccagctatc tgtcacttta ttgagaagat agtggaaaag gaaggtggct cctacaaatg
      481 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
      541 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      601 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
      661 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggcctacgcg
      721 tgctcgaggg taccatgagg ataaatatct ctttgtctag tctctttgag agactgagca
      781 aattgtcatc ccgatccatc gcaattacct gcggcgtagt cttggcaagt gcaatagcat
      841 tcccaattat ccgaagagac tatcaaacgt tccttgaagt cggtccgagc tatgccccac
      901 agaacttccg aggctatatc atcgtttgtg tcttgtcact ttttaggcaa gagcagaaag
      961 ggttggcaat ctatgacagg cttccagaaa agaggcgttg gcttgccgac ttgccgtttc
     1021 gtgaagggac gaggccatcc ataacctccc acattataca gcgtcagcgt actcagctgg
     1081 tagatcaaga gtttgcaacc agagaactta tcgataaggt gatcccacgt gtgcaagcaa
     1141 gacacacaga caaaactttt ctcagtacgt caaaatttga gtttcatgca aaggccatct
     1201 tccttctccc ctctatccct attaatgatc ctcttaacat tccctcacac gatacggtaa
     1261 gaagaaccaa gcgtgagatc gctcacatgc atgattacca tgattgcact ctgcacttgg
     1321 ctcttgctgc tcaggatggt aaggaagttt tgaagaaggg gtggggccag cgtcacccac
     1381 tggccggacc aggagtccca ggccctccta ctgagtggac cttcctttac gcaccaagga
     1441 acgaagagga ggccagagtt gtcgagatga tagtcgaagc tagtattggc tacatgacta
     1501 acgatcctgc tggtaagatt gtcgaaaatg ccaagtcagg ttgagaattc ggatcgtata
     1561 cctgcaggat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     1621 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     1681 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     1741 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     1801 gtcatctatg ttactagatc ggggagctct ggcaggatat atgcggttgt aattcatttt
     1861 tattgtctaa atttctgtat ttgtttgttt gcaattagat ccacgttgtg tctcaaaatc
     1921 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
     1981 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgttcga
     2041 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     2101 atgtcgggca atcaggtgcg acaatctacc gattgtatgg gaagcccgat gcgccagagt
     2161 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     2221 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     2281 atgatgcatg gttactcacc actgcgatcc cagggaaaac agcattccag gtattagaag
     2341 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     2401 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     2461 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     2521 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     2581 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     2641 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     2701 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     2761 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     2821 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     2881 gcggctttgt tgaatcaatt gatcttcttg aaatcctttt tttctgcgcg taatctgctg
     2941 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3001 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3061 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3121 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3181 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3241 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3301 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3361 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3421 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3481 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3541 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3601 cgcctttgag tgatctctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
     3661 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
     3721 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
     3781 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
     3841 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
     3901 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
     3961 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
     4021 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
     4081 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
     4141 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
     4201 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
     4261 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
     4321 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
     4381 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
     4441 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
     4501 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
     4561 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
     4621 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
     4681 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
     4741 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag
     4801 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
     4861 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
     4921 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
     4981 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
     5041 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
     5101 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
     5161 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
     5221 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
     5281 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
     5341 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
     5401 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
     5461 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
     5521 gatcgagcta gttgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
     5581 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
     5641 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
     5701 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     5761 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     5821 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     5881 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaagatctct ttcc
//
