LOCUS       sequence-443884-        7205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This binary vector expresses mNeonGreen with a modified IR pepper 
            golden mosaic virus (PepGMV) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7205)
  TITLE     Curtis lab plasmids
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLSU2/mIR{PepGMV}:mNeonGreen:NosT
FEATURES             Location/Qualifiers
     source          1..7205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..195
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             687..1481
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(741..760)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1351..1370
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      2839..3091
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3267..3286)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3278..4093
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3278..4093
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3351..3370)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4179..4767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4668..4687
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             5009..5638
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6067..7140
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELVDWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatgatat cgcatgctat gcagcatttt tttggtgtat
      421 gtgggcccca aatgaagtgc aggtcaaacc ttgacagtga cgacaaatcg ttgggcgggt
      481 ccagggcgaa ttttgcgaca acatgtcgag gctcagcagc tgaaaattca aacgcgctag
      541 tcaatgctat caatctgtcg tgttcacacg gcatcaaacg gtcaccaatg acgtcaatgg
      601 gcttccttag tcaatataaa aaccaacggc tcagacttac cagcggcagg tatttgtagt
      661 acatccaaca ctgccctgat cttcgcatga ttgaacaaga tggattgcac gcaggttctc
      721 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
      781 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
      841 acctgtccgg tgccctgaat gaactccagg acgaggcagc gcggctatcg tggctggcca
      901 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
      961 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     1021 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     1081 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     1141 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     1201 ccaggctcaa ggcgcggatg cccgacggcg aggatctcgt cgtgacacat ggcgatgcct
     1261 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     1321 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     1381 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     1441 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc cagatcaccg
     1501 tcgccgacac ctaataaagt cggctaatct atgtgattga gtgtgtcttg actttgttat
     1561 tttgcatgtt tccaatgtca tttagtaacg aaataaacgt tatcctcttc taaaagcagg
     1621 ctgtgttttc ggcaaacatc gccacccatc gctagttttt ctaaaagtgt tctaagcggg
     1681 cctggtaata atctatacga gcttatattt ctaatcattg ccgaaaaatc ctgtttcgaa
     1741 ataattttgt aactctcttt aatatcacca cgagcaagct tggaattctc tgcctgacgc
     1801 acagtgatgc tctccccggt gcgtgaatcc attatttgcg cagttgatgt ggaggaagat
     1861 agagcagccg cagtttaggt ctattggatc ccggatggcc gcgatttttc ggagtccttg
     1921 ggggaccact cagaatagag ggctgagatt aaatagataa agagagggga ccaccaatta
     1981 tttgattttg actgaccaat cacatcccgc ctgtcaagtc tagatatttg tgccttggtg
     2041 accaaggtgt ggtcctataa atgcacgtgc atgtgcattt agacctttaa ttcaaaacgc
     2101 gtgctcgagg gtaccgaatt catggtttcc aaaggtgagg aagataatat ggctagttta
     2161 cctgccacac atgagctaca catattcggc agcataaacg gtgtagattt tgatatggtt
     2221 ggacaaggga caggtaatcc taatgatgga tatgaggagt tgaacctcaa atctaccaag
     2281 ggggaccttc aattctcccc atggatccta gtccctcaca ttggatacgg cttccaccag
     2341 tacttaccat acccagatgg aatgtcccct ttccaagcag ccatggttga cggttccggg
     2401 taccaggtac ataggacaat gcaatttgag gacggggctt ctctgactgt gaattaccga
     2461 tatacctatg aaggcagtca cataaagggc gaagcacaag tgaagggcac cggatttcct
     2521 gccgacgggc ctgtaatgac taatagcttg accgccgcag attggtgcag gtcaaaaaag
     2581 acctacccaa atgacaagac aataatctct acatttaagt ggagctacac tacagggaat
     2641 ggtaaacgat atagatcaac tgctagaacc acatatacct tcgctaaacc tatggcagcc
     2701 aattatctga agaaccagcc catgtatgtg ttccgaaaga ctgaactcaa gcatagcaaa
     2761 accgaactca acttcaaaga gtggcaaaag gctttcactg acgtgatggg aatggacgag
     2821 ttatataagt aactgcagga tcgttcaaac atttggcaat aaagtttctt aagattgaat
     2881 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
     2941 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
     3001 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
     3061 tcgcgcgcgg tgtcatctat gttactagat cggggagctc tggcaggata tatgcggttg
     3121 taattcattt ttattgtcta aatttctgta tttgtttgtt tgcaattaga tccacgttgt
     3181 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     3241 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
     3301 gtcttgttcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     3361 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctac cgattgtatg ggaagcccga
     3421 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     3481 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     3541 ccgtactcct gatgatgcat ggttactcac cactgcgatc ccagggaaaa cagcattcca
     3601 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     3661 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     3721 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     3781 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     3841 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     3901 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     3961 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     4021 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     4081 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     4141 ttgacgggac ggcggctttg ttgaatcaat tgatcttctt gaaatccttt ttttctgcgc
     4201 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4261 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4321 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4381 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4441 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4501 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4561 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4621 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4681 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4741 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4801 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4861 aaccgtatta ccgcctttga gtgatctctg aagaaaccga gcgccgccgt ctaaaaaggt
     4921 gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag
     4981 taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg
     5041 cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc
     5101 cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg
     5161 ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa
     5221 ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc
     5281 tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga
     5341 catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg
     5401 aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga
     5461 ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg
     5521 cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg
     5581 cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt
     5641 taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc
     5701 gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg
     5761 gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa
     5821 ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc
     5881 aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga
     5941 acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg
     6001 cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga
     6061 atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat
     6121 gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa
     6181 gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa
     6241 ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat
     6301 tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg
     6361 gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt
     6421 ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac
     6481 gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg
     6541 aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc
     6601 cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac
     6661 accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta
     6721 tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg
     6781 gagtacatcg agatcgagct agttgattgg atgtaccgcg agatcacaga aggcaagaac
     6841 ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt
     6901 ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg
     6961 atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag
     7021 ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc
     7081 ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa
     7141 tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaagatctc
     7201 tttcc
//
