LOCUS       sequence-443892-       11156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Assembled Tn10 with CP25 promoter driving gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11156)
  AUTHORS   Alker AT, Farrell MV, Demko AM, Purdy TN, Adak S, Moore BS, Sneed 
            JM, Paul VJ, Shikuma NJ
  TITLE     Linking bacterial tetrabromopyrrole biosynthesis to coral 
            metamorphosis.
  JOURNAL   ISME Commun. 2023 Sep 19;3(1):98. doi: 10.1038/s43705-023-00309-6.
  PUBMED    37726481
REFERENCE   2  (bases 1 to 11156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ISME 
            Commun."; date: "2023-09-19"; volume: "3(1):98. doi"; pages: " 
            10.1038/s43705-023-00309-6"
COMMENT     Sequence Label: pMMK803
FEATURES             Location/Qualifiers
     source          1..11156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1452..1471)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1463..2278
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1463..2278
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1536..1555)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(2324..2351)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(2367..2438)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(2648..2695)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(6182..6204)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6193..6209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6217..6223,6224..6239,6240..6245))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        6467..6544
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6545..7627
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6564..6583)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        7734..7838
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7839..7907,7908..8699)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8057..8076)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(8756..8775)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8916..8938
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9077..9096
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            9777..9886
                     /note="incP origin of transfer"
     CDS             9919..10290
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      join(10908..11156,1..140)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 aaaaaaaaga atatataagg cttttaaagc ttttaaggtt taacggttgt ggacaacaag
       61 ccagggatgt aacgcactga gaagccctta gagcctctca aagcaatttt gagtgacaca
      121 ggaacactta acggctgaca tgggaattcg gtatacatca ctttatttaa aacgatgccc
      181 attttgttga ttatttattt ttcagcgcaa ttgataggcc aaattcccgc aacggtgtgg
      241 gtgctattta ccgaaaatcg ttttggatgg aatagcatga tggttggctt ttcattagcg
      301 ggtcttggtc ttttacactc agtattccaa gcctttgtgg caggaagaat agccactaaa
      361 tggggcgaaa aaacggcagt actgctcgga tttattgcag atagtagtgc atttgccttt
      421 ttagcgttta tatctgaagg ttggttagtt ttccctgttt taattttatt ggctggtggt
      481 gggatcgctt tacctgcatt acagggagtg atgtctatcc aaacaaagag tcatcagcaa
      541 ggtgctttac agggattatt ggtgagcctt accaatgcaa ccggtgttat tggcccatta
      601 ctgtttgctg ttatttataa tcattcacta ccaatttggg atggctggat ttggattatt
      661 ggtttagcgt tttactgtat tattatcctg ctatcgatga ccttcatgtt aacccctcaa
      721 gctcagggga gtaaacagga gacaagtgct tagttatttc gtcaccaaat gatgttattc
      781 cgcgaaatat aatgaccctc ttgataaccc aagagggcat tttttacgat aaagaagatt
      841 tagcttcaaa taaaacctat ctattttatt tatctttcaa gctcaataaa aagccgcggt
      901 aaatagcaat aaattggcct tttttatcgg caagctcttt taggtttttc gcatgtattg
      961 cgatatgcat aaaccagcca ttgagtaagt ttttaagcac atcatcatca taagctttcc
     1021 tgacggaatg ttaattctcg ttgaccctga gcactgatga atcccctaat gattttggta
     1081 aaaatcatta agttaaggtg gatacacatc ttgtcatatg atcccggatc cggctgtaat
     1141 ccgggcagcg caacggaaca ttcatcagtg taaaaatgga atcaataaag ccctgcgcag
     1201 cgcgcagggt cagcctgaat acgcgtggcc gcctaggccg cggccgccgg gcaagtacga
     1261 catcacccgg cccaaggcgg caggctgact cacgttaagg gattttggtc atgactgatc
     1321 cttcaactca gcaaaagttc gatttattca acaaagccac gttgtgtctc aaaatctctg
     1381 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     1441 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctccaggcc
     1501 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     1561 cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt
     1621 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     1681 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     1741 tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata
     1801 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     1861 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgcctcg ctcaggcgca
     1921 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     1981 gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt
     2041 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     2101 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     2161 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     2221 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     2281 agaattggtt aattggttgt aacacgcggc cgctcggtat aaacgcagaa aggcccaccc
     2341 gaaggtgagc cagtgtgact ctagtagaga gcgttcaccg acaaacaaca gataaaacga
     2401 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggtgta ctgcagtgca
     2461 ctagtggttc gtaacatctc tgtaactgct tgagacgtgg actgttgtga tatgccatat
     2521 atgcagtggt gttcgccggc gctggttccg gctgtcttgc ttagttgtga gtcgccaggc
     2581 agtcatcggt atgatctgta catgattcgt cagtgtgagc accactgacg agcagatttc
     2641 cagccagcaa aaaacccctc aagacccgtt tagaggcccc aaggggttat gctagttagg
     2701 atttatttgt atagttcatc catgccgtgt gtaatcccag cagccgtgac aaactcaaga
     2761 aggaccatgt ggtcgcgctt ttcgttggga tctttcgaaa gggcagattg agtggacagg
     2821 taatggttgt ctggtaacag cacaggccca tcgccaattg gggtgttttg ttggtaatgg
     2881 tcggctagtt gaacgcttcc atcctcaatg ttgtgtctga tcttgaagtt aaccttgata
     2941 ccgttctttt gcttgtcggc cataatgtac acattatggg agttatagtt atattccagt
     3001 ttgtggccta aaatatttcc atcttcctta aagtcaatac cctttaactc gattctatta
     3061 acaagggtat caccctcgaa cttcacttcg gcgcgtgtct tatagttgcc gtcatcctta
     3121 aaaaagatgg ttctctcctg tacatagcct tcgggcattg cactcttgaa aaagtcgtgc
     3181 tgtttcatgt gatctgggta gcgcgcaaag cactgaaccc catacccgaa cgtagtgacc
     3241 agtgttggcc atggaacagg tagtttaccc gtggtgcaaa taaatttaag ggtcagcttg
     3301 ccatatgttg cgtcgccttc accctctcca ctgacagaga acttgtgccc attaacatca
     3361 ccgtctaatt ccacgaggat tgggacaact cctgtgaaaa gctcttctcc tttactcata
     3421 tgtaatatct cctctgtttc tgtataacag tactatgtga ttataccagc cccctcacta
     3481 catgtcaaga ataaactgcc aaagcgttgt attgcgacga attgccacgt tgtcgcagtg
     3541 tctgtactgt gggttaatgt ctgtgatcgc cgtcgtgagt tcgtgtcatc cgcgcatgat
     3601 tcagatggtc ctggagatcg ttgcttgtac cggctgtttg tggctctacc acgtctcatc
     3661 agtctagatg cgaattcagg ggcctgtcag accaagttta ctcatatata ctttagattg
     3721 atttctgaaa gcgaccaggt gctcgcggcc gcacgcgtat tcaggctgac cctgcgcgct
     3781 gcgcagggct ttattgattc catttttaca ctgatgaatg ttccgttgcg ctgcccggat
     3841 tacagccgga tccgggatca tatgacaaga tgtgtatcca ccttaactta atgattttta
     3901 ccaaaatcat taggggattc atcagtgctc agggtcaacg agaattaaca ttccgtcagg
     3961 aaagctttaa gttggttctc ttggatcaat ttgctgacaa tggcgtttac cttaccagta
     4021 atgtattcaa ggctaatttt ttcaagttca ttccaaccaa tgataggcat cacttcttgg
     4081 atagggataa ggtttttatt attatcaata atataatcaa gataatgttc aaatatactt
     4141 tctaaggcag accaaccatt tgttaaatca gtttttgttg tgatgtaggc atcaatcata
     4201 attaattgct gcttataaca ggcactgagt aattgttttt tatttttaaa gtgatgataa
     4261 aaggcacctt tggtcaccaa cgcttttccc gagatcgatc tcatctattg aaacagcttg
     4321 atagcctttt tcaacaaaca atattcgtgc tgagttaacc agtgattgat aggtactctt
     4381 aaaattttct tgttgatgat ttttattttc catgatagat ttaaaataac ataccgtcag
     4441 tatgtttatg gtatcatgat gatgtggtcg tgacaatctt aagaacattt aggttatttt
     4501 atgtatattg aacagcattc tcgctatcaa aataaagcta ataacatcca attaagatat
     4561 gatgataagc agtttcatac aacggttatc aaagatgttc tattatggat tgaacataat
     4621 ttagatcagt ctttactgct tgatgatgtg gcgaataaag cgggttatac caagtggtat
     4681 tttcagcggc tgttcaaaaa gtaacagggg tcacactggc tagctatatt cgtgctcgtc
     4741 gtttgacgaa agcggctgtt gagttgaggt tgacgaaaaa aactatcctt gagatcgcat
     4801 taaaatatca atttgattcc caacaatctt ttacacgtcg atttaagtac atttttaagg
     4861 ttacaccaag ttattatcgg cgtaataaat tatgggaatt ggaggcaatg cactgagaga
     4921 tcccctcata atttccccaa agcgtaacca tgtgtgaata aattttgagc tagtagggtt
     4981 gcagccacga gtaagtcttc ccttgttatt gtgtagccag aatgccgcaa aacttccatg
     5041 cctaagcgaa ctgttgagag tacgtttcga tttctgactg tgttagcctg gaagtgcttg
     5101 tcccaacctt gtttctgagc atgaacgccc gcaagccaac atgttagttg aagcatcagg
     5161 gcgattagca gcatgatatc aaaacgctct gagctgctcg ttcggctatg gcgtaggcct
     5221 agtccgtagg caggactttt caagtctcgg aaggtttctt caatctgcat tcgcttcgaa
     5281 tagatattaa caagttgttt gggtgttcga atttcaacag gtaagttagt tgctagaacc
     5341 catggctcct ttgccgacgc tgagtagatt ttaggtgacg ggtggtgaca atgagtccgt
     5401 gtcgagcgct gattttttcg gcctttagag cgagatttat acaatagaat ttggcatgag
     5461 attggattgc ttttagtcag cctcttatag cctaaagtct ttgagtgact agatgacata
     5521 tcatgtaagt tgctgatagg tttccagttt tccgctccta ggtctgcata ttgtactttt
     5581 cctcttactc gacttaacca gtaccaaccc agcttctcaa cggatttata ccatggcact
     5641 ttaaagccag catcactgac aatgagcggt gtggtgttac tcggtagaat gctcgcaagg
     5701 tcggctagaa attggtcatg agctttcttt gaacattgct ctgaaagcgg gaacgctttc
     5761 tcataaagag taacagaacg accgtgtagt gcgactgaag ctcgcaatac cataagtcgt
     5821 ttttgctcac gaatatcaga ccagtcaaca agtacaatgg gcatcgtatt gcccgaacag
     5881 ataaagctag catgccaacg gtatacagcg agtcgctctt tgtggaggtg acgattacct
     5941 aacaatcggt cgattcgttt gatgttatgt tttgttctcg ctttggttgg caggttacgg
     6001 ccaagttcgg taagagtgag agttttacag tcaagtaatg cgtggcaagc caacgttaag
     6061 ctgttgagtc gttttaagtg taattcgggg cagaattggt aaagagagtc gtgtaaaata
     6121 tcgagttcgc acatcttgtt gtctgattat tgatttttcg cgaaaccatt tgatcctgtt
     6181 tcctgtgtga aattgttatc cgctcacaat tccacacatt atacgagccg atgattaatt
     6241 gtcaacagct catttcagaa tatttgccag aaccgttatg atgtcggcgc aaaaaacatt
     6301 atccagaacg ggagtgcgcc ttgagcgaca cgaattatgc agtgatttac gacctgcaca
     6361 gccataccac agcttccgat ggctgcctga cgccagaagc attggtgcac cgtgcagtcg
     6421 atgataagct gtcaaacatg agaattcccg ggagagctcg gtacccgaca ccatcgaatg
     6481 gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt
     6541 gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac
     6601 cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga
     6661 agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa
     6721 acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat
     6781 tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt
     6841 agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt
     6901 cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc
     6961 ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat
     7021 tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca
     7081 ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc
     7141 tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga
     7201 ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc
     7261 cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga
     7321 gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag
     7381 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
     7441 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
     7501 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
     7561 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     7621 gcagtgagcg caacgcaatt aatgtaagtt agctcactca ttaggcaccc caggctttac
     7681 actttatgct tccgacctgc agatctgcag gtggcacttt tcggggaaat gtgcgcggaa
     7741 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7801 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7861 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7921 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7981 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8041 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     8101 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8161 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8221 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8281 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8341 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
     8401 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8461 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8521 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8581 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8641 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8701 tgtcagacca agtttactca tatatacttt agattgattt atggtgcact ctcagtacaa
     8761 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
     8821 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     8881 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     8941 ttcaccgtca tcaccgaaac gcgcgaggca gcaaggagat ggcgcccaac agtcccccgg
     9001 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     9061 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     9121 cggtgatgcc ggccacgatg cgtccggcgt agaggatcct ttttgtccgg tgttgggttg
     9181 aaggtgaagc cggtcggggc cgcagcgggg gccggctttt cagccttgcc cccctgcttc
     9241 ggccgccgtg gctccggcgt cttgggtgcc ggcgcgggtt ccgcagcctt ggcctgcggt
     9301 gcgggcacat cggcgggctt ggccttgatg tgccgcctgg cgtgcgagcg gaacgtctcg
     9361 taggagaact tgaccttccc cgtttcccgc atgtgctccc aaatggtgac gagcgcatag
     9421 ccggacgcta acgccgcctc gacatccgcc ctcaccgcca ggaacgcaac cgcagcctca
     9481 tcacgccggc gcttcttggc cgcgcgggat tcaacccact cggccagctc gtcggtgtag
     9541 ctctttggca tcgtctctcg cctgtcccct cagttcagta atttcctgca tttgcctgtt
     9601 tccagtcggt agatattcca caaaacagca gggaagcagc gcttttccgc tgcataaccc
     9661 tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc acgatataca
     9721 ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg tcagccgggc
     9781 aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt cccttattcg
     9841 cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccggctac cgccggcgta
     9901 acagatgagg gcaagcggat ggctgatgaa accaagccaa ccaggaaggg cagcccacct
     9961 atcaaggtgt actgccttcc agacgaacga agagcgattg aggaaaaggc ggcggcggcc
    10021 ggcatgagcc tgtcggccta cctgctggcc gtcggccagg gctacaaaat cacgggcgtc
    10081 gtggactatg agcacgtccg cgagctggcc cgcatcaatg gcgacctggg ccgcctgggc
    10141 ggcctgctga aactctggct caccgacgac ccgcgcacgg cgcggttcgg tgatgccacg
    10201 atcctcgccc tgctggcgaa gatcgaagag aagcaggacg agcttggcaa ggtcatgatg
    10261 ggcgtggtcc gcccgagggc agagccatga cttttttagc cgctaaaacg gccggggggt
    10321 gcgcgtgatt gccaagcacg tccccatgcg ctccatcaag aagagcgact tcgcggagct
    10381 ggtgaagtac atcaccgacg agcaaggcaa gaccgagcgc ctgggtcacg tgcgcgtcac
    10441 gaactgcgag gcaaacaccc tgcccgctgt catggccgag gtgatggcga cccagcacgg
    10501 caacacccgt tccgaggccg acaagaccta tcacctgctg gttagcttcc gcgcgggaga
    10561 gaagcccgac gcggagacgt tgcgcgcgat tgaggaccgc atctgcgctg ggcttggctt
    10621 cgccgagcat cagcgcgtca gtgccgtgca tcacgacacc gacaacctgc acatccatat
    10681 cgccatcaac aagattcacc cgacccgaaa caccatccat gagccgtatc gggcctaccg
    10741 cgccctcgct gacctctgcg cgacgctcga acgggactac gggcttgagc gtgacaatca
    10801 cgaaacgcgg cagcgcgttt ccgagaaccg cgcgaacgac atggagcggc acgcgggcgt
    10861 ggaaagcctg gtcggctgga tccggccacg atgcgtccgg cgtagaggat ctgaagatca
    10921 gcagttcaac ctgttgatag tacgtactaa gctctcatgt ttcacgtact aagctctcat
    10981 gtttaacgta ctaagctctc atgtttaacg aactaaaccc tcatggctaa cgtactaagc
    11041 tctcatggct aacgtactaa gctctcatgt ttcacgtact aagctctcat gtttgaacaa
    11101 taaaattaat ataaatcagc aacttaaata gcctctaagg ttttaagttt tataag
//
