LOCUS       sequence-443893-        5986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retrovirus-mediated gene transfer into mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Kendall J, Liu Q, Bakleh A, Krasnitz A, Nguyen KC, Lakshmi B, Gerald
            WL, Powers S, Mu D
  TITLE     Oncogenic cooperation and coamplification of developmental 
            transcription factor genes in lung cancer.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16663-8. Epub 2007 Oct
            9.
  PUBMED    17925434
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2007-10-16"; volume: "104(42)"; pages: 
            "16663-8. Epub 2007 Oct 9"
COMMENT     Sequence Label: pMSCV-Zeo
FEATURES             Location/Qualifiers
     source          1..5986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             57..571
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     CDS             complement(618..992)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1013..1512)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1061..1080)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     1454..1477
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(1547..1963)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(1563..1579)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(1595..1617)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    2030..2371
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             2435..2951
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(3151..3170)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3298..3320)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3514..3533)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3633..3655
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3693..3711)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3779..3883
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3884..3952,3953..4744)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4102..4121)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4915..5503
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5404..5423
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5657..5674
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5791..5812
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5827..5832,5833..5850,5851..5857)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5865..5881
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aatattcgac tcctaccctc agaatgttgt tctccaactt caagaaactg ttacccatga
       61 aagacccccg ctgacgggta gtcaatcact cagaggagac cctcccaagg aacagcgaga
      121 ccacaagtcg gatgcaactg caagagggtt tattggatac acgggtaccc gggcgacgca
      181 gtctatcgga ggactggcgc gccgagtgag gggttgtggg ctcttttatt gagctcgggg
      241 agcagaagcg cgcgaacaga agcgagaagc gaactgattg gttagttcaa ataaggcaca
      301 gggtcatttc aggtccttgg ggcaccctgg aaacatctga tggttctcta gaaactgctg
      361 agggcgggac cgcatctggg gaccatctgt tcttggccct gagccggggc aggaactgct
      421 taccacagat atcctgtttg gcccatattc tgctgtctct ctgttcctaa ccttgatctg
      481 aacttctcta ttctcagtta tgtattttcc atgccttgca aaatggcgtt acttaagcta
      541 gcttgccaaa cctacaggtg gggtctttca ttcccccctt tttctggaga ctaaataaaa
      601 tcttttattt tatcgattca gtcctgctcc tcggccacga agtgcacgca gttgccggcc
      661 gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc cgatctcggt catggccggc
      721 ccggaggcgt cccggaagtt cgtggacacg acctccgacc actcggcgta cagctcgtcc
      781 aggccgcgca cccacaccca ggccagggtg ttgtccggca ccacctggtc ctggaccgcg
      841 ctgatgaaca gggtcacgtc gtcccggacc acaccggcga agtcgtcctc cacgaagtcc
      901 cgggagaacc cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc gcgcgcggtg
      961 agcaccggaa cggcactggt caacttggcc atggtaagct tgggctgcag gtcgaaaggc
     1021 ccggagatga ggaagaggag aacagcgcgg cagacgtgcg cttttgaagc gtgcagaatg
     1081 ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct gagcccgccc ctgagcccgc
     1141 ccccggaccc accccttccc agcctctgag cccagaaagc gaaggagcaa agctgctatt
     1201 ggccgctgcc ccaaaggcct acccgcttcc attgctcagc ggtgctgtcc atctgcacga
     1261 gactagtgag acgtgctact tccatttgtc acgtcctgca cgacgcgagc tgcggggcgg
     1321 gggggaactt cctgactagg ggaggagtag aaggtggcgc gaaggggcca ccaaagaacg
     1381 gagccggttg gcgcctaccg gtggatgtgg aatgtgtgcg aggccagagg ccacttgtgt
     1441 agcgccaagt gcccagcggg gctgctaaag cgcatgctcc agactgcctt gggaaaagcg
     1501 cctcccctac ccggtagaat tcgttaacct cgagagatct aattccggcg cctagagaag
     1561 gagtgagggc tggataaagg gaggatcgag gcggggtcga acgaggaggt tcaaggggga
     1621 gagacggggc ggatggagga agaggaggcg gaggcttagg gtgtacaaag ggcttgaccc
     1681 agggaggggg gtcaaaagcc aaggcttccc aggtcacgat gtaggggacc tggtctgggt
     1741 gtccatgcgg gccaggtgaa aagaccttga tcttaacctg ggtgatgagg tctcggttaa
     1801 aggtgccgtc tcgcggccat ccgacgttaa aggttggcca ttctgcagag cagaaggtaa
     1861 cccaacgtct cttcttgaca tctaccgact ggttgtgagc gatccgctcg acatctttcc
     1921 agtgacctaa ggtcaaactt aagggagtgg taacagtctg gccctaattt tcagacaaat
     1981 acagaaacac agtcagacag agacaacaca gaacgatgct gcagcgctgc agcagacaag
     2041 acgcgcggct tcggttccaa accgaaagca aaaattcaga cggaggcggg aactgtttta
     2101 ggttctcgtc tcctaccaga accacatatc ctgacggggt cggattccac atcgactccc
     2161 ttcctcaggt cgggccacaa aaacggcccc caaagtccct gggacgtctc ccagggttgc
     2221 ggccgggtgt tcagaactcg tcagttccac cacgggtccg ccagatacag agctagttag
     2281 ctaactagta cagacgcagg cgcaaacatt agatgccggc acaaacacgc acaaagacag
     2341 agacagacac gaaacgaccg ctggccagct tacctcccgg cgggggggtc ggtggtccct
     2401 gggcaggggt ctccaaatct cggtggaacc tccaaatgaa agacccccga ggtgggcagt
     2461 caatcaatct gaggagaccc tcccaaggat cagcgagtcc acgattcgga tgcaaacagc
     2521 aagaggcttt attgggaata cgggtacccg ggcgacgcag tctatcggag gactggcgcg
     2581 ccgagtgagg ggttgtgggc tcttttattg agctcgggga gcagaagcgc gcgaacagaa
     2641 gcgagaagcg aactgattgg ttagttcaaa taaggcacag ggtcatttca ggtccttggg
     2701 gcaccctgga aacatctgat ggttctctag aaactgctga gggcgggacc gcatctgggg
     2761 accatctgtt cttggccctg agccggggca ggaactgctt accacagata tcctgtttgg
     2821 cccatattct gctgtctctc tgttcctaac cttgatctga acttctctat tctcagttat
     2881 gtattttcca tgccttgcaa aatggcgtta cttaagctag cttgccaaac ctacaggtgg
     2941 ggtctttcat tccccccttt ttctggagac taaataaaat cttttatttt atctatggct
     3001 cgtactctat aggcttcagc tggtgatatt gttgagtcaa aactagagcc tggaccactg
     3061 atatcctgtc tttaacaaat tggactaatc gcctctacgc cggacgcatc gtggccggca
     3121 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     3181 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     3241 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcgccgtcgt
     3301 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     3361 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     3421 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     3481 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     3541 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     3601 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     3661 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     3721 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     3781 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3841 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3901 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3961 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4021 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4081 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     4141 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     4201 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4261 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4321 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4381 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     4441 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4501 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4561 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4621 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4681 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4741 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4801 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4861 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4921 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4981 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5041 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5101 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5161 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5221 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5281 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5341 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5401 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5461 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5521 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5581 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     5641 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     5701 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     5761 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     5821 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     5881 atttcacaat tctccccagc tctttctgcg ctgtccataa tgaactattt caggagtatt
     5941 ggagtatgtg gattcaaaac agtggctaat tgagtcagga ttactt
//
