LOCUS       sequence-443897-        9919 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 9919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-CMV-Galpha-i1 ONE-GO
FEATURES             Location/Qualifiers
     source          1..9919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             123..143
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             249..764
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    778..1366
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(831..851)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1249..1260)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1573..2206
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2334..2350)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2334..2350)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2347..2369)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2358..2374
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2382..2388,2389..2406,2407..2412))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2427..2448
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2565..2582)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2736..3324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2816..2835)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3495..4286,4287..4355))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4118..4137
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4356..4460)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    4508..4642
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4545..4564)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4599..4618
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4810..5443
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5490..5615
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6112..6345
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6530..6574
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6837..6952
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        7010..7313
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7314..7517
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7468..7488
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7514..7538
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(8013..8015,8016..8018,8019..8729)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8058..8079)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8319..8338)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8666..8687
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(9445..9919,1..100,101..103,104..112)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9612..9629)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9839..9858
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
       61 cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccgtaccaa
      121 agatggggag tagcaagagc aagacgtcga attccggaat caagcttggt ggatcttctc
      181 gaggttacta ccatggtatt tgggtgggtg aagaaggtcg actttctcga ggtggcaccg
      241 gtggatccat ggtcttcaca ctcgaagatt tcgttgggga ctggcgacag acagccggct
      301 acaacctgga ccaagtcctt gaacagggag gtgtgtccag tttgtttcag aatctcgggg
      361 tgtccgtaac tccgatccaa aggattgtcc tgagcggtga aaatgggctg aagatcgaca
      421 tccatgtcat catcccgtat gaaggtctga gcggcgacca aatgggccag atcgaaaaaa
      481 tttttaaggt ggtgtaccct gtggatgatc atcactttaa ggtgatcctg cactatggca
      541 cactggtaat cgacggggtt acgccgaaca tgatcgacta tttcggacgg ccgtatgaag
      601 gcatcgccgt gttcgacggc aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca
      661 aaattatcga cgagcgcctg atcaaccccg acggctccct gctgttccga gtaaccatca
      721 acggagtgac cggctggcgg ctgtgcgaac gcattctggc gtaaacgcgt ctggaacaat
      781 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
      841 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
      901 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
      961 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     1021 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     1081 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     1141 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     1201 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     1261 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     1321 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct
     1381 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     1441 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     1501 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     1561 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     1621 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     1681 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     1741 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     1801 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     1861 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     1921 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     1981 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     2041 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2101 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2161 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     2221 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct
     2281 tgacattgct agcgtttacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     2341 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2401 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     2461 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     2521 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2581 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2641 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2701 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2761 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2821 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2881 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2941 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3001 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3061 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3121 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     3181 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3241 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3301 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3361 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3421 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3481 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3541 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3601 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3661 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3721 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3781 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3841 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3901 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3961 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4021 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4081 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4141 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4201 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4261 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4321 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4381 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4441 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     4501 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4561 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4621 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     4681 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     4741 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     4801 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     4861 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     4921 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     4981 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     5041 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     5101 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     5161 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     5221 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     5281 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5341 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     5401 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     5461 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     5521 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     5581 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     5641 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     5701 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     5761 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     5821 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     5881 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     5941 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     6001 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     6061 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     6121 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     6181 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     6241 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     6301 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     6361 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     6421 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     6481 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     6541 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     6601 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     6661 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     6721 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     6781 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta
     6841 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     6901 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     6961 attacaggga cagcagagat ccagtttatc gataagcttg ggagttccgc gttacataac
     7021 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     7081 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     7141 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     7201 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     7261 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     7321 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     7381 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     7441 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     7501 tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
     7561 gttttgacct ccatagaaga caccgactct actagaggat ctatttccgg tgaattcctc
     7621 gagactagtt ctagagcggc cgcggatcca tgggctgcac actgagcgct gaggacaagg
     7681 cggccgtgga gcgcagcaag atgatcgacc gcaacctccg ggaggacgga gagaaggcag
     7741 cgcgcgaggt caagctgctg ctgctgggtg ctggtgaatc cgggaagagc acaattgtga
     7801 agcagatgaa aattatccac gaggctggct actcagagga agagtgtaag cagtacaaag
     7861 cagtggtcta cagcaacacc atccagtcca tcattgccat cattagagcc atggggagat
     7921 tgaaaatcga ctttggagac gctgctcgtg cggatgatgc tcgccaactc ttcgtgcttg
     7981 ctggggctgc agaggaaggc tttatgaccg cgatggtgag caagggcgag gagctgttca
     8041 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     8101 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     8161 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctga
     8221 tgtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     8281 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     8341 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     8401 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     8461 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     8521 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     8581 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca
     8641 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     8701 tcactctcgg catggacgag ctatacaagg cggagctcgc cggcgtcata aagagactgt
     8761 ggaaggacag cggtgtgcaa gcctgcttca acagatcccg ggagtaccag ctgaacgatt
     8821 cggcggcgta ctacctgaat gacttggaca gaatagcaca accaaattac atcccaaccc
     8881 agcaggatgt tctcagaact agagtgaaaa cgacgggaat tgtggaaacc cactttactt
     8941 tcaaagatct tcattttaaa atgtttgacg tgggaggcca gagatcagag cggaagaagt
     9001 ggattcactg ctttgaaggc gtgactgcca tcatcttctg tgtggccctg agtgactatg
     9061 acctggttct tgctgaggat gaagaaatga accggatgca cgaaagcatg aagctgttcg
     9121 atagcatatg taacaacaag tggtttacgg acacatccat catccttttc ctgaacaaga
     9181 aggacctctt cgaagagaag atcaaaaaga gtcccctcac gatatgctat ccagaatatg
     9241 caggctcaaa cacatatgaa gaggcggctg cgtatatcca gtgtcagttt gaagacctca
     9301 ataaaaggaa ggacacaaag gaaatttaca cccacttcac ttgcgccacg gatacgaaga
     9361 atgtgcagtt tgtgttcgat gctgtaacgg acgtcatcat aaagaataac ctaaaagact
     9421 gtggtctctt ctaagcggcc gccgcccctc tccctccccc ccccctaacg ttactggccg
     9481 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     9541 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     9601 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     9661 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     9721 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     9781 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     9841 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     9901 gggatctgat ctggggcct
//
