LOCUS       sequence-443919-        8785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 8785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: MEK1 (S218D,S222D)-pcw107
FEATURES             Location/Qualifiers
     source          1..8785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    115..348
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             533..577
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        808..1018
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        830..1026
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      877..1012
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1058..1660
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(1058..1077)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1554..1574
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1703..1820
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1878..2378
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2297..2315
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    2399..2423
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3624..3648)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3680..4268
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3733..3753)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4151..4162)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4340..4573
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4651..4772
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4688..4707)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4742..4761
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4812..4947
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4967..4986)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4968..4986)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4996..5013)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4996..5012)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5005..5027)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5154..5609
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5241..5260)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5451..5472
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5635..5739
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5740..5808,5809..6600)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5958..5977)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6771..7359
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7260..7279
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7513..7530
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7647..7668
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7683..7688,7689..7706,7707..7713)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7721..7737
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7726..7748
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7745..7761
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7745..7761
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7780..7800
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7782..7800
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7828..8054
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8055..8235
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8282..8407
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
       61 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
      121 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
      181 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
      241 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
      301 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
      361 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
      421 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
      481 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
      541 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
      601 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
      661 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
      721 tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc
      781 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat
      841 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      901 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      961 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     1021 tgcaaaaagc tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt
     1081 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     1141 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     1201 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     1261 cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     1321 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     1381 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     1441 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     1501 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     1561 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     1621 gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac
     1681 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     1741 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     1801 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     1861 ggctcgaggc ctgcaggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg
     1921 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct
     1981 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga
     2041 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt
     2101 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa
     2161 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg
     2221 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc
     2281 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg
     2341 gctccctcgt tgaccgaatc accgacctct ctccccaggg gctagcatcg atggatcaac
     2401 aagtttgtac aaaaaagttg gcatgagcgg cggcggcacc atgcccaaga agaagccgac
     2461 gcccatccag ctgaacccgg cccccgacgg ctctgcagtt aacgggacca gctctgcgga
     2521 gaccaacttg gaggccttgc agaagaagct ggaggagcta gagcttgatg agcagcagcg
     2581 aaagcgcctt gaggcctttc ttacccagaa gcagaaggtg ggagaactga aggatgacga
     2641 ctttgagaag atcagtgagc tgggggctgg caatggcggt gtggtgttca aggtctccca
     2701 caagccttct ggcctggtca tggccagaaa gctaattcat ctggagatca aacccgcaat
     2761 ccggaaccag atcataaggg agctgcaggt tctgcatgag tgcaactctc cgtacatcgt
     2821 gggcttctat ggtgcgttct acagcgatgg cgagatcagt atctgcatgg agcacatgga
     2881 tggaggttct ctggatcaag tcctgaagaa agctggaaga attcctgaac aaattttagg
     2941 aaaagttagc attgctgtaa taaaaggcct gacatatctg agggagaagc acaagatcat
     3001 gcacagagat gtcaagccct ccaacatcct agtcaactcc cgtggggaga tcaagctctg
     3061 tgactttggg gtcagcgggc agctcatcga cgacatggcc aacgacttcg tgggcacaag
     3121 gtcctacatg tcgccagaaa gactccaggg gactcattac tctgtgcagt cagacatctg
     3181 gagcatggga ctgtctctgg tagagatggc ggttgggagg tatcccatcc ctcctccaga
     3241 tgccaaggag ctggagctga tgtttgggtg ccaggtggaa ggagatgcgg ctgagacccc
     3301 acccaggcca aggacccccg ggaggcccct tagctcatac ggaatggaca gccgacctcc
     3361 catggcaatt tttgagttgt tggattacat agtcaacgag cctcctccaa aactgcccag
     3421 tggagtgttc agtctggaat ttcaagattt tgtgaataaa tgcttaataa aaaaccccgc
     3481 agagagagca gatttgaagc aactcatggt tcatgctttt atcaagagat ctgatgctga
     3541 ggaagtggat tttgcaggtt ggctctgctc caccatcggc cttaaccagc ccagcacacc
     3601 aacccatgct gctggcgtct aattgccaac tttcttgtac aaagtggtta ccggttagta
     3661 atgaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3721 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3781 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3841 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3901 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3961 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4021 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     4081 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4141 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4201 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4261 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     4321 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     4381 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     4441 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     4501 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     4561 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     4621 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     4681 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4741 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     4801 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4861 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4921 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     4981 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     5041 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     5101 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     5161 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5221 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5281 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5341 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5401 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5461 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5521 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5581 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     5641 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5701 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5761 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5821 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5881 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5941 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     6001 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6061 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6121 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6181 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6241 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6301 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6361 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6421 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6481 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6541 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6601 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6661 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6721 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6781 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6841 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6901 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6961 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7021 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7081 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7141 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7201 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7261 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7321 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7381 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7441 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7501 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7561 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7621 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7681 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7741 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     7801 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     7861 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     7921 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     7981 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     8041 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8101 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8161 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8221 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     8281 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     8341 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     8401 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     8461 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     8521 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     8581 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     8641 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     8701 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     8761 gacctggagg aggagatatg aggga
//
