LOCUS       sequence-443921-       10693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector expressing rice E3 ligase Tir1 in mammalian cells 
            with selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10693)
  AUTHORS   Xie L, Dong P, Chen X, Hsieh TS, Banala S, De Marzio M, English BP, 
            Qi Y, Jung SK, Kieffer-Kwon KR, Legant WR, Hansen AS, Schulmann A, 
            Casellas R, Zhang B, Betzig E, Lavis LD, Chang HY, Tjian R, Liu Z
  TITLE     3D ATAC-PALM: super-resolution imaging of the accessible genome.
  JOURNAL   Nat Methods. 2020 Apr;17(4):430-436. doi: 10.1038/s41592-020-0775-2.
            Epub 2020 Mar 16.
  PUBMED    32203384
REFERENCE   2  (bases 1 to 10693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0775-2"; journalName: "Nat Methods"; date: 
            "2020-04"; volume: "17"; issue: "4"; pages: "430-436"
COMMENT     Sequence Label: pLX_lenti_osTir1_9Myc_P2A_Bsd
FEATURES             Location/Qualifiers
     source          1..10693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2650..2861
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2877..2886
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2931..4652
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="TYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVG
                     NCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEEL
                     RMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVE
                     DRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLA
                     KILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPL
                     CAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRV
                     FPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLC
                     ILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIA
                     FAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGC
                     QVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             4680..4709
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4716..4745
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4752..4781
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4800..4829
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4836..4865
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4872..4901
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4926..4982
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4983..5381
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    5406..5994
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5459..5479)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5877..5888)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5997..6013)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5998..6014)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6519..6699
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6725..6742)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6731..6955
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7001..7429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7088..7107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7298..7319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7438..7458)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7603..7737
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7664..7683
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7799..7846
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7865..8239
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8369..8490
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8406..8425)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8460..8479
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8539..8555)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8539..8555)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8552..8574)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8563..8579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8587..8593,8594..8611,8612..8617))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8632..8653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8770..8787)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8941..9529)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9021..9040)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9700..10491,10492..10560))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10323..10342
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10561..10665)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctagg tcttgaaagg agtgggaatt ggctccggtg
     2641 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     2701 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     2761 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     2821 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggttc tagaccgcca
     2881 ccatggcatt acaaaaaaaa ttagaagaat tagaattaga tgaaggcggc acgtacttcc
     2941 cggaggaggt ggtggagcac atcttcagct tcctgccggc gcagcgcgac cgcaacacgg
     3001 tctcgctcgt ctgcaaggtg tggtacgaga tcgagaggct gagccgccgc ggcgtcttcg
     3061 tgggcaactg ctacgccgtg cgcgccggcc gcgtcgccgc gcggttcccc aacgtgcggg
     3121 cgctcacggt gaaggggaag ccccacttcg ccgacttcaa cctcgtgccc cccgactggg
     3181 gcggctacgc ggggccgtgg atcgaggcgg ccgcgagggg atgccacggc ctggaggagc
     3241 tcaggatgaa gcggatggtg gtgtccgacg agagcctcga gctgctggct cgctcgttcc
     3301 cgcggttcag ggctcttgtt cttatcagct gcgaggggtt cagcactgac gggctagccg
     3361 ccgtcgcgag ccattgcaag cttctgaggg agttggattt gcaggaaaat gaagtggagg
     3421 atcgagggcc taggtggctt tcctgcttcc ctgattcctg cacatcactt gtctcattga
     3481 attttgcctg catcaaaggg gaggttaatg ctggttcact ggagagactt gttagcaggt
     3541 ccccaaacct gcggagtttg aggctgaatc gatctgtatc ggtagataca cttgcaaaga
     3601 tactactgcg tacccctaac ttggaggatt tggggacagg gaatttgaca gatgacttcc
     3661 aaactgagtc ctactttaag cttaccagtg ctctggagaa atgcaagatg ttgaggagtt
     3721 tgtctggatt ctgggatgct tctcctgttt gcctgtcatt tatctacccc ctgtgtgctc
     3781 aactgacagg attgaacttg agctatgcac ccacacttga tgcttctgac cttacaaaaa
     3841 tgattagccg ctgtgtgaag ctccaacgcc tttgggtact ggattgtatc tcggacaaag
     3901 gcttgcaagt ggtggcctcc agttgcaaag acttgcaaga actcagggta tttccatcag
     3961 atttctacgt agctggttat tctgcagtga cagaggaggg acttgttgca gtatccttgg
     4021 gctgtccaaa actgaactca ctactgtact tctgtcacca aatgactaat gctgcactag
     4081 ttactgtcgc caagaactgt ccaaatttca cacgattcag actttgtatt cttgagccag
     4141 ggaagcctga tgttgtgaca agccaaccat tagatgaagg ctttggagct attgttcgtg
     4201 agtgcaaggg attacaacgt ttgtcaatat ctggtcttct cacagacaaa gttttcatgt
     4261 atattgggaa atatgcaaaa caacttgaga tgctttctat agcatttgct ggtgacagtg
     4321 ataagggtat gatgcatgtt atgaatggat gcaagaattt aaggaaactg gagataagag
     4381 atagcccgtt tggtgatgct gcactcttgg ggaattttgc taggtacgag acaatgcgat
     4441 ccctttggat gtcatcttgc aatgtcacgt taaaggggtg ccaagtcctt gcgtcaaaga
     4501 tgccgatgct caatgttgag gtcataaatg agcgggatgg tagcaatgaa atggaggaaa
     4561 accatggaga tctgcctaaa gtggagaaat tatatgtgta ccgcacaact gctggggcga
     4621 gggatgatgc accaaatttt gttaaaatcc tagtcgactc cggttctgct gctagtggtg
     4681 aacaaaagtt gatttctgaa gaagatttga acggtgaaca aaagctaatc tccgaggaag
     4741 acttgaacgg tgaacaaaaa ttaatctcag aagaagactt gaacggatcc tctagaggtg
     4801 aacaaaagtt gatttctgaa gaagatttga acggtgaaca aaagctaatc tccgaggaag
     4861 acttgaacgg tgaacaaaaa ttaatctcag aagaagactt gaacggatcc actagcggat
     4921 ccggcgcaac aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac
     4981 cgatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa
     5041 tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg
     5101 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg
     5161 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg
     5221 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc
     5281 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga
     5341 ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agaattcgat atcaagctta
     5401 tcggtaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5461 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5521 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5581 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5641 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5701 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5761 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     5821 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5881 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5941 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     6001 accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     6061 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     6121 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6181 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     6241 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     6301 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     6361 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     6421 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     6481 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     6541 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6601 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6661 ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga
     6721 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     6781 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     6841 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     6901 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     6961 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     7021 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     7081 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7141 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7201 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     7261 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     7321 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     7381 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     7441 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7501 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     7561 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     7621 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7681 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7741 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     7801 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     7861 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     7921 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     7981 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     8041 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     8101 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     8161 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     8221 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
     8281 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     8341 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     8401 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8461 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     8521 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8581 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8641 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8701 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8761 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8821 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8881 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8941 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9001 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9061 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9121 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9181 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9241 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9301 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9361 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     9421 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9481 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9541 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9601 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9661 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9721 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9781 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9841 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9901 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9961 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10021 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10081 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10141 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10201 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10261 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10321 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10381 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10441 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10501 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10561 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10621 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10681 agtgccacct gac
//
