LOCUS       sequence-443925-        4693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the Rotavirus SA11 delta42C NSP1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4693)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-NSP1delta42CSA11
FEATURES             Location/Qualifiers
     source          1..4693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1321..1339)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1335..1382
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(1450..1468)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1451..1468)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1758..1777)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1987..2006)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2106..2128
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2166..2184)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2252..2356
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2357..2425,2426..3217)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2575..2594)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3388..3976
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3877..3896
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4130..4147
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4232..4250
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcccaattac attaaatgct gctgcattta agtttgatga aaataatctt tattatgttt
       61 ttgggttata tgagaaatca gtcagtgata tatatgctcc atatagaatt gttaacttta
      121 taaatgaatt tgataaatta ttgcttgatc atattaactt tacaagaatg tccaatctac
      181 caatagagtt gagaaaccat tacgcaaaga aatacttcca attatcaaga ctgccatcat
      241 caaaactaaa gcaaatttac ttttcagatt ttactaaaga aactgtgatt tttaatactt
      301 atacaaaaac gccaggaaga tcaatataca gaaatgtaac tgaatttaat tggagagatg
      361 aattggagct ttattctgat ttaaaaaatg ataagaataa attaattgct gcaatgatga
      421 cgagtaagta tactcggttc tatgctcatg ataataattt tggaaggttg aaaatgacaa
      481 tatttgagtt gggacatcat tgtcagccta actacgtggc atctaatcac ccaggcaatg
      541 cttccgatat ccagtactgt aaatggtgta atataaaata ttttcttagt aaaattgatt
      601 ggcggattcg tgatatgtat aatttattga tggaatttat taaggattgt tataaaagta
      661 atgttaacgt tggacattgt agttctgttg aaaacatata tcctttaatt aaaagattaa
      721 tttggagttt gtttactaat cacatggatc aaacaattga agaagtgttt aatcacatgt
      781 cgccagtgtc agttgaaggt acgaatgtca tcatgttgat tcttggattg aatattagtt
      841 tgtataatga aattaagcgc actttgaatg tagatagcat accaatggta cttaatttaa
      901 atgaattcag tagtatagtt aaatcaatta gcagtaaatg gtattgatag taaaatgttg
      961 atgaattgga taaattgcca atgtcaataa aatcaacgga ggaactgatt gaaatgaaga
     1021 attctggaac tttaactgaa gaatttgagc tactgatctc caactcagaa gatgacaatg
     1081 agtgaaatta tgtcactatc taattataca gtatttagcc atcacaagac cgtccagact
     1141 agagtagcgc ctagctggca aaatactgtg aaccgggtcg gcatggcatc tccacctcct
     1201 cgcggtccga cctgggcatc cgaaggagga cgtcgtccac tcggatggct aagggagagc
     1261 tcaaaaaaaa ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
     1321 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     1381 tgctgaaagg aggaactata tccggatcga gatcctctag agtcgacctg caggcatgca
     1441 agcttgtatt ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta
     1501 attgtagccg cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact
     1561 gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac
     1621 cttctgagtt tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag
     1681 ggtcatcgct agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     1741 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     1801 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     1861 actgttgggc gccatctcct tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     1921 ctactactgg gctgcttctt aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
     1981 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     2041 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     2101 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     2161 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     2221 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     2281 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     2341 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     2401 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     2461 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     2521 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     2581 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     2641 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     2701 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     2761 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     2821 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     2881 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     2941 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     3001 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     3061 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     3121 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     3181 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     3241 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     3301 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     3361 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3421 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3481 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3541 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3601 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3661 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3721 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3781 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3841 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3901 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3961 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4021 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     4081 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     4141 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     4201 tcattaatgc agggggatat cgatcccggg ttaatacgac tcactatagg cttttttttg
     4261 aaaagtcttg tgttagccat ggctactttt aaagatgcat gctttcatta tcgtagatta
     4321 actgctttaa atcggagatt atgcaacatt ggtgcaaatt ctatttggat gccagttcct
     4381 gatgcgaaaa ttaaggggtg gtgtttagaa tgttgtcaaa tagctgattt aacccattgt
     4441 tatggttgct cattgccgca tgtttgcaaa tggtgtgttc agaacagaag atgcttcctt
     4501 gacaatgaac ctcatttgct taagcttaga actgtgaaac atccaattac caaagacaaa
     4561 ttacagtgta tcatagactt gtacaatata atatttccaa ttaatgataa agtaattaga
     4621 aaatttgaaa gaatgataaa gcaaagagaa tgtaggaatc aatataaaat tgaatggtat
     4681 aatcatttgc tgc
//
