LOCUS       sequence-443926-        4654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the Rotavirus RF NSP1 recoded protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4654)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-NSP1RF(ioSA11)
FEATURES             Location/Qualifiers
     source          1..4654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        291..309
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     291..308
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(377..424)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     420..438
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     promoter        complement(2163..2181)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2266..2283)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2437..3025)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2517..2536)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3196..3987,3988..4056))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3819..3838
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4057..4161)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4229..4247
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4285..4307)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4407..4426
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(4636..4654,1)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg
       61 atctggctag cgatgaccct gctgattggt tcgctgacca tttccgggtg cgggacggcg
      121 ttaccagaaa ctcagaaggt tcgtccaacc aaaccgactc tgacggcagt ttacgagaga
      181 gatgataggg tctgcttcag taagccagat gctacacaat taggcttgta catattgtcg
      241 ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga
      301 cactatagaa tacaagcttg catgcctgca ggtcgactct agaggatctc gatccggata
      361 tagttcctcc tttcagcaaa aaacccctca agacccgttt agaggcccca aggggttatg
      421 ctagttattg ctcagcggtg gcagcagcca actcagcttc ctttcgggct ttgttagcag
      481 ccggatcctt ttttttgagc tctcccttag ccatccgagt ggacgacgtc ctccttcgga
      541 tgcccaggtc ggaccgcgag gaggtggaga tgccatgccg acccggggtc acattttatg
      601 ctgcctaggc gctactctag tgcagacggt cttgtgctgg cgtagttgtg accatattta
      661 ctcaacatct gaaagttcca gatcatactc gtcgattagc tcgtttcgtt tatttaaagt
      721 tctcagttga tttgttgttg aggttgtcat tggggtttct cgcatatacc ttgtgtcaaa
      781 ccagtcaaat atcattgcgc tgagaatttt tttagtatcg ttcatattca gaatccgtgg
      841 tacttttcca tccatattct gcattactaa tcctcgtact tcccaattta cctcatagtc
      901 taggagaacg tattcagtgc cattgatatc aacgggctca agataattaa ataacatttc
      961 cacggcctca ttccattttt ctgttttgca gattaggaat aaatcaggta tatatttata
     1021 aactgattct tgtgaagaac aatgtccaac attgccattt gatttcataa gtgctcttat
     1081 aaagtccatt acattattgt agatcttgcg cagtctaaag tcttcccaga ccttgtaatg
     1141 attattgatt tgacaccact tgcagttatg cacattgagt gcacaggatt cgtggctact
     1201 aaagatgtag ttgggcttca ccagtcgact tacaatgccg aatttatatt tggtaaataa
     1261 cttgcagcgt tggcttactg aatagtgttc ggtgtatgag ctattcatga tcgaaatata
     1321 gttgcgtgcg tctacgatca atgtgcaagc cttattccac tcgcagtcgt tgatatgtat
     1381 acttatacaa tttcttacta cttgcataag tgaggttggg gaatgtctat cttccattaa
     1441 attatcacta aaatcacttc ttgacaaccg ctttttaccc ttgctcagaa atctactctt
     1501 gctgaaatat cttaaagcat attcctgctg taatatcacc ggtaaatgtg acattctgtg
     1561 gaaatttcta tcatctagta gcaatttatc atatttttct aataaattaa tgaattgaaa
     1621 aggcgtttgg ttttcatgct ccatgcaatc gtaatacccg aatatataat aaacattatc
     1681 ctccaagttg atggtcagtg cctgcagggt gattggcata agcagatgat tgtaccattc
     1741 caacaaatat tcgttgcgac atttgcgctg ttttactgag tttataaatt tatttactaa
     1801 tttttcgttt ataggaaata aggtttcgta cattgtgagt aatccttcga tatcttcttt
     1861 agttaccacg tctttgaacg tccgcattct taataaatgg ggttcttcat ctaaaaagca
     1921 ccgattatat tgagaacacc actgacacac atggtagaga gcacatccgc ggcagtaagt
     1981 aagatttgtg tattggcaac aatctagaca ccatcccttg tactttgtca ttggtgctgg
     2041 cctccattca tcatttgctc ctagtttgag cactagtgaa ttcagtttat tcagccgttt
     2101 gtagtgataa caggcatcct tgaaagttgc catggctcac acaagacttt tcataaataa
     2161 agcctatagt gagtcgtatt aacccgggat cgatatcccc ctgcattaat gaatcggcca
     2221 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     2281 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2341 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2401 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2461 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2521 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2581 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2641 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2701 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2761 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2821 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     2881 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2941 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3001 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3061 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3121 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3181 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3241 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3301 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3361 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3421 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3481 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3541 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3601 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3661 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3721 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3781 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3841 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3901 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3961 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4021 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4081 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4141 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     4201 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     4261 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     4321 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     4381 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     4441 ttcgacgctc tcccttatgc gactcctgca ttaagaagca gcccagtagt aggttgaggc
     4501 cgttgagcac cgccgccgca aggaatggtg caaggagatg gcgcccaaca gtcccccggc
     4561 cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc
     4621 ccgatcttcc ccatcggtga tgtcggcgat atag
//
