LOCUS       sequence-443930-        4640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the Rotavirus DS-1 NSP1 recoded protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4640)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-NSP1DS1(ioSA11)
FEATURES             Location/Qualifiers
     source          1..4640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(276..1067,1068..1136))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     899..918
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1137..1241)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1309..1327
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1365..1387)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1487..1506
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1716..1735
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2025..2043
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2025..2042
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(2111..2158)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     2154..2172
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     promoter        complement(3883..3901)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3986..4003)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(4157..4640,1..105))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4237..4256)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
       61 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      121 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      181 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      241 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      301 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
      361 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
      421 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
      481 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
      541 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
      601 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
      661 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
      721 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
      781 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
      841 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
      901 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
      961 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1021 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1081 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1141 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1201 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1261 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     1321 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     1381 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     1441 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     1501 attgtactga gagtgcacca ttcgacgctc tcccttatgc gactcctgca ttaagaagca
     1561 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg caaggagatg
     1621 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc
     1681 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca
     1741 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgg
     1801 ctagcgatga ccctgctgat tggttcgctg accatttccg ggtgcgggac ggcgttacca
     1861 gaaactcaga aggttcgtcc aaccaaaccg actctgacgg cagtttacga gagagatgat
     1921 agggtctgct tcagtaagcc agatgctaca caattaggct tgtacatatt gtcgttagaa
     1981 cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat
     2041 agaatacaag cttgcatgcc tgcaggtcga ctctagagga tctcgatccg gatatagttc
     2101 ctcctttcag caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctagtt
     2161 attgctcagc ggtggcagca gccaactcag cttcctttcg ggctttgtta gcagccggat
     2221 cctttttttt gagctctccc ttagccatcc gagtggacga cgtcctcctt cggatgccca
     2281 ggtcggaccg cgaggaggtg gagatgccat gccgacccgg ggtcacactt ttaatgctgc
     2341 ctaggcgcta ctctagtgca gagagtctca tggtggcgag aatttcttta aaatcattca
     2401 atatcggata tacctgaatc gtgttctgct gttctatctt tctcgtataa ttgttttagt
     2461 ttgttgacag taaacgtagt cattggtgta tttctcataa atcttatgtc aaaccaatca
     2521 tagattatta ttcttaatat tagtatgacg tcgcttaacg tgagtatttt agggattttt
     2581 ccgatactat taattaatac attcataacg ttccaattga tctcgtaatt gaggagtatg
     2641 taattgttct cgttcagctt gatgggctca agctgattaa acatttcagt cactaacata
     2701 ttccactcat tttccttaca aacatttaac acatctttta tggattcata gattttttct
     2761 tcgctactgc aatgacctac gtataaatta ctttttacga gtgctcttat aaaattgaag
     2821 atgtcgttat acacattctt gatacggaag tcattccaaa cagtactgtt gttgttaatg
     2881 gaacaccatt tacagttgtg gacttctgtt gctaacgagt tgtggttcga cgcgacgtag
     2941 tttggcttga tcagtttcga agcaattttc agcttatatt gggtaaacat ccgacatctg
     3001 ggtgaaatgt tgtagaattc tgtgtggcta cttttcagaa tgtcgaaata gtcgcttata
     3061 ttccgaatta tattgcacgc tttgttccat tctacggaca tattactaca attccttatc
     3121 acctgtaaag taaagttggg gttatgtaaa ttatctaata tattatctga aaaatgtaaa
     3181 atttcaatac atttgcggcg ttctgttatg aagcgagatt tggaaaaata tctaagtgca
     3241 tactcttgtt gtaatgttaa tggtaaaaat gccattctgt caaaatttat actatcaagt
     3301 agtaacatat cgtatttact aattaaattc gtgaatgaga agggagtctg attaatttta
     3361 tgcatatcgt cataataacc aaatatataa taaatatctc catccaactc tatggacaat
     3421 gactgcaacg taatcggcat caggaaatga ttgtaccatt gggttaaata ttcgttgcga
     3481 catttgtgct gctttatggt attggcaaat ttatgaacta tttttttatt tataggaaat
     3541 aaagtgttat acatattaat gaggttttct aaatcactct tagtgatttc gttccgaaaa
     3601 gttctcatgc gtaataaatg cgggtcgtca tctaaaaagc accgattata ttgactacac
     3661 cattcgcaca cgtggtatat caggcagccc tgacaatatg ttaaatccgt gtgttgacaa
     3721 cagtctagac accatccttt tctcttggtc agggtcgatg gtctccagac gtcattagcg
     3781 cctagtttta gtactgcatt atttaatttg tttaattttt tatattgata gcaagcatct
     3841 ttaaaagtag ccatggcttc cacaagactt ttcaaaaaaa agcctatagt gagtcgtatt
     3901 aacccgggat cgatatcccc ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3961 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4021 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4081 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4141 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4201 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4261 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4321 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4381 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4441 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4501 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4561 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     4621 tgctgaagcc agttaccttc
//
