LOCUS       sequence-443932-        4084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the hybrid protein combining 35 amino acids from Rotavirus 
            SA11 NSP1 protein with the recoded Green Lantern protein carrying an
            ER-localization signal at the N- and C-termini.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4084)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-35aaNSP1SA11-Green Lantern(io)
FEATURES             Location/Qualifiers
     source          1..4084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     38..57
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     267..286
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        576..594
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     576..593
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(662..709)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     705..723
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     promoter        complement(1878..1896)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1981..1998)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2152..2740)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2232..2251)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2911..3702,3703..3771))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3534..3553
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3772..3876)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3944..3962
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4000..4022)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
       61 agagtgcacc attcgacgct ctcccttatg cgactcctgc attaagaagc agcccagtag
      121 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcaaggagat ggcgcccaac
      181 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
      241 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
      301 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatctg gctagcgatg
      361 accctgctga ttggttcgct gaccatttcc gggtgcggga cggcgttacc agaaactcag
      421 aaggttcgtc caaccaaacc gactctgacg gcagtttacg agagagatga tagggtctgc
      481 ttcagtaagc cagatgctac acaattaggc ttgtacatat tgtcgttaga acgcggctac
      541 aattaataca taaccttatg tatcatacac atacgattta ggtgacacta tagaatacaa
      601 gcttgcatgc ctgcaggtcg actctagagg atctcgatcc ggatatagtt cctcctttca
      661 gcaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctagt tattgctcag
      721 cggtggcagc agccaactca gcttcctttc gggctttgtt agcagccgga tccttttttt
      781 tgagctctcc cttagccatc cgagtggacg acgtcctcct tcggatgccc aggtcggacc
      841 gcgaggaggt ggagatgcca tgccgacccg gttcacagta ttttgccagc taggcgctac
      901 tctagtctgg acggtcttgt gatggctaaa tactgtataa ttagatagtg acataatttt
      961 aaagttcgtc ttttttgtat aattcgtcca tgtcgtgtgt tattccggcg gctgttaccc
     1021 gttcttttaa taccatatga tctctttttt cgtttgggtc tttagataat ttactttgat
     1081 gtgaaaggta atggttgtcg ggtaatagta ctggtccgtc tcctatagga gtattttgtt
     1141 gatagtgatc tgcgagttga acacctccgt cttctacatt atgtctagtt ttaaaatttg
     1201 cttttatacc gtttttttgt ttgtcggcag tgatatatac tttatgtgaa ttaaagttat
     1261 attctaattt gtgtcctaaa atgtttccat cttctttaaa atcaatacct tttaaaacaa
     1321 ttcggttaac aagagtatct ccttcaaatt ttacttcggc tctcgtttta taagttccgt
     1381 catctttaaa acttattgtt ctttcttgta catatccttc tggcattgca cttttaaaaa
     1441 aatcgtgttg tttcatgtgg tcggggtaac gtgcaaagca tgctactccg tatcctaatg
     1501 ttgttactaa tgttggccaa ggtacgggta attttcctgt tgtgcaaata aattttaatg
     1561 taagtttacc atttgttgcg tcaccttccc cttcgcctcg tactgagaat ttatgtccgt
     1621 tgacatcacc gtcgagttcg actaatatag gtacgacgcc cgtaaataat tcttctcctt
     1681 tacttaccat gtctgcggca gcgagtccta gtaatcctaa aagtagtggt acgcttaaga
     1741 gcatatcagg aactggcatc caaatagaat ttgcaccaat gttgcataat ctccgattta
     1801 aagcagttaa tctacgataa tgaaagcatg catctttaaa agtagccatg gctaacacaa
     1861 gacttttcaa aaaaaagcct atagtgagtc gtattaaccc gggatcgata tccccctgca
     1921 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     1981 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2041 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     2101 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2161 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2221 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2281 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2341 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2401 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2461 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2521 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2581 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2641 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2701 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2761 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2821 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2881 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2941 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     3001 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     3061 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3121 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3181 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3241 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3301 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3361 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3421 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3481 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3541 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3601 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3661 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3721 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3781 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3841 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3901 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     3961 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     4021 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     4081 gtca
//
